AIM: To determine whether intra-arterial infusion of triolein emulsion has biochemical and histopathologic effect on rabbit liver.METHODS: An emulsion of 0.2 mL triolein in 20 mL of saline was infused into either the ...AIM: To determine whether intra-arterial infusion of triolein emulsion has biochemical and histopathologic effect on rabbit liver.METHODS: An emulsion of 0.2 mL triolein in 20 mL of saline was infused into either the hepatic arteries of nine rabbits(group 1) or the superior mesenteric arteries of 12 rabbits(group 2). Five rabbits infused with 20 mL of normal saline were used as a control group(group 3). The serum aspartate aminotransferase(AST) and alanine aminotransferase(ALT) levels were measured to evaluate liver function in each group just before the infusion, at 2 h, day 1, day 4, and day 7 following infusion. Each rabbit in all of the groups was infused with Evans blue on day 7 to evaluate changes in vascular permeability, and obtain the stained area of the hepatic surface. If the stained area was not available, the anteroinferior portion of the right hepatic lobe was selected. The obtained tissues were examined by light, electron and confocal microscopy. The changes in AST and ALT levels at each time point were calculated and statistically analyzed using a mixed linear model. A P value < 0.05 was regarded as statistically significant. RESULTS: In group 1(hepatic artery group), both the AST and ALT serum levels increased significantly on day 1(P = 0.0016 and P < 0.0001, respectively) compared with the control group, followed by a decrease thereafter. In group 2(portal vein group), the AST level increased on day 4(P = 0.0095), while the ALT level increased significantly on day 1(P < 0.0001), and decreased thereafter, as compared with the control. For the remainder of the examination days, there were no significant changes in the AST and ALT levels(P > 0.05). Only three rabbits in each group showed hepatic surface staining with the Evans blue dye. Light and electron microscopic findings showed no specific changes in the selected hepatic tissues. Confocal microscopic examination with transferase-mediated dUTP nick-end labeling stain revealed lack of hepatocyte apoptosis in any of the groups. There were no differences in the results between group 1 and group 2.CONCLUSION: Infusion of triolein emulsion into rabbit livers revealed a minimal transient decrease of liverfunction,and no specific histopathologic changes.展开更多
BACKGROUND The infusion of triolein emulsion(TE)induced increased vascular permeability and a negligible and temporary decrease in liver function without specific histopathological damage.AIM To assess changes in doxo...BACKGROUND The infusion of triolein emulsion(TE)induced increased vascular permeability and a negligible and temporary decrease in liver function without specific histopathological damage.AIM To assess changes in doxorubicin concentration according to the percentage of TE infused via a hepatic artery to study the vascular permeability in the rabbit liver.METHODS Thirty-nine healthy rabbits were divided into five groups according to the concentration of emulsified triolein infused into the hepatic arteries:Group 0,saline infusion(control group,n=5);group 1,0.3%TE(n=13);group 2,0.6%TE(n=6);group 3,0.9%TE(n=8);and group 4,1.5%TE(n=6).Doxorubicin(2.4 mg/kg)was infused immediately after TE injection via the hepatic arteries.After 2 h,the livers were harvested,and doxorubicin concentrations were calculated fluorometrically.The doxorubicin concentrations were compared between TE groups and the control group,and the optimal concentrations within the TE groups were calculated.Statistical analysis was performed using the nonparametric Mann-Whitney U test and Kruskal-Wallis test.P<0.05 were considered statistically significant.RESULTS In the liver,doxorubicin concentrations were 2.06,2.07,2.16 and 1.66 times higher in groups 1 through 4,respectively,and significantly higher in the TE groups than in the control group(all P<0.05).However,there were no significant differences in the mean doxorubicin concentrations between the four TE groups(P=0.642).In the lungs,the mean doxorubicin concentrations were not significantly different between the control and TE groups(P>0.05).CONCLUSION TE infusion into the hepatic arteries significantly increased the doxorubicin concentration approximately twofold but was not different between the TE groups.These findings suggest that TE infusion might be a useful adjuvant treatment of liver cancers.展开更多
A novel triolein-embedded adsorbent for re-moval of persistent organic pollutants (POPs) from water was developed. In this paper, taking dieldrin and endrin as the target pollutants, different factors (temperature, io...A novel triolein-embedded adsorbent for re-moval of persistent organic pollutants (POPs) from water was developed. In this paper, taking dieldrin and endrin as the target pollutants, different factors (temperature, ion strength, pH, and concentration of humic acid) that affect the adsorption rate were investigated. Adsorption isotherms of these two pollutants fitted to Freundlich model well identi-fied the partition mechanism between the adsorbent and water. However, the equilibrium constant of dieldrin was larger than that of endrin due to larger octanol-water coeffi-cient of dieldrin. Adsorption rate of dieldrin increased with the increase of temperature, the decrease of ion strength, pH and concentration of humic acid, whereas adsorption rate of endrin decreased with the increase of temperature and pH. Humic acid firstly increased, and then decreased the adsorp-tion rate of endrin. Ion strength hardly affected the adsorp-tion rate of endrin.展开更多
A novel triolein-embedded activated carbon composite adsorbent was developed. Experiments were car-ried out in areas such as the preparation method, the char-acterization of physicochemical properties, and the adsorp-...A novel triolein-embedded activated carbon composite adsorbent was developed. Experiments were car-ried out in areas such as the preparation method, the char-acterization of physicochemical properties, and the adsorp-tion behavior of the composite adsorbent in removing diel-drin from aqueous solution. Results suggested that the novel composite adsorbent was composed of the supporting acti-vated carbon and the surrounding triolein-embedded cellu-lose acetate membrane. The adsorbent was stable in water, for no triolein leakage was detected after soaking the ad-sorbent for five weeks. The adsorbent had good adsorption capability to dieldrin, which was indicated by a residual di-eldrin concentration of 0.204 μg·L?1. The removal efficiency of the composite adsorbent was higher than the traditional activated carbon adsorbent.展开更多
Passive sampling methods have been widely used for more than 20 years because they can provide information on time-weighted average concentrations(CTWA) of hydrophobic organic contaminants in water.Many factors affect...Passive sampling methods have been widely used for more than 20 years because they can provide information on time-weighted average concentrations(CTWA) of hydrophobic organic contaminants in water.Many factors affect the efficiency and applicability of a passive sampling process.Among others,selection of the sampler design and choice of the supporting membrane material are important factors that determine the performance of passive samplers.In this work,the influences of some critical properties of membranes,such as membrane density,hydrophilicity of the membrane surface,and chemical and mechanical resistances,on the uptake of organic chlorinated pesticides(OCPs) were evaluated.Four membrane materials,namely cellulose acetate(CA),cellulose acetate butyrate(CAB),a cellulose acetate-cellulose triacetate blend(CA-CTA),and cellulose triacetate(CTA),and their triolein-embedded samplers were selected for the study.The four membranes are all hydrophilic;CAB has the highest contact angle and CA has the lowest.CTA is chemically and mechanically resistant,and has better tensile strength than the other membranes have.CAB does not have good chemical resistance and dissolves in hexane/dichloromethane solvent.CA and CTA alone showed lower uptake rates for the selected OCPs than with triolein-embedded passive samplers,indicating that triolein increased the accumulation capacity.Triolein-embedded CA(TECAM),CA-CTA(TECA-CTA),CTA(TECTA),and CAB(TECAB) as passive samplers showed similar uptake patterns;TECTA showed the highest uptake rates and TECAB showed the poorest accumulation.In summary,TECTA gave the best performance among all the tested composite semipermeable membranes.展开更多
文摘AIM: To determine whether intra-arterial infusion of triolein emulsion has biochemical and histopathologic effect on rabbit liver.METHODS: An emulsion of 0.2 mL triolein in 20 mL of saline was infused into either the hepatic arteries of nine rabbits(group 1) or the superior mesenteric arteries of 12 rabbits(group 2). Five rabbits infused with 20 mL of normal saline were used as a control group(group 3). The serum aspartate aminotransferase(AST) and alanine aminotransferase(ALT) levels were measured to evaluate liver function in each group just before the infusion, at 2 h, day 1, day 4, and day 7 following infusion. Each rabbit in all of the groups was infused with Evans blue on day 7 to evaluate changes in vascular permeability, and obtain the stained area of the hepatic surface. If the stained area was not available, the anteroinferior portion of the right hepatic lobe was selected. The obtained tissues were examined by light, electron and confocal microscopy. The changes in AST and ALT levels at each time point were calculated and statistically analyzed using a mixed linear model. A P value < 0.05 was regarded as statistically significant. RESULTS: In group 1(hepatic artery group), both the AST and ALT serum levels increased significantly on day 1(P = 0.0016 and P < 0.0001, respectively) compared with the control group, followed by a decrease thereafter. In group 2(portal vein group), the AST level increased on day 4(P = 0.0095), while the ALT level increased significantly on day 1(P < 0.0001), and decreased thereafter, as compared with the control. For the remainder of the examination days, there were no significant changes in the AST and ALT levels(P > 0.05). Only three rabbits in each group showed hepatic surface staining with the Evans blue dye. Light and electron microscopic findings showed no specific changes in the selected hepatic tissues. Confocal microscopic examination with transferase-mediated dUTP nick-end labeling stain revealed lack of hepatocyte apoptosis in any of the groups. There were no differences in the results between group 1 and group 2.CONCLUSION: Infusion of triolein emulsion into rabbit livers revealed a minimal transient decrease of liverfunction,and no specific histopathologic changes.
基金Biomedical Research Institute,Pusan National University Hospital,No.2018B008。
文摘BACKGROUND The infusion of triolein emulsion(TE)induced increased vascular permeability and a negligible and temporary decrease in liver function without specific histopathological damage.AIM To assess changes in doxorubicin concentration according to the percentage of TE infused via a hepatic artery to study the vascular permeability in the rabbit liver.METHODS Thirty-nine healthy rabbits were divided into five groups according to the concentration of emulsified triolein infused into the hepatic arteries:Group 0,saline infusion(control group,n=5);group 1,0.3%TE(n=13);group 2,0.6%TE(n=6);group 3,0.9%TE(n=8);and group 4,1.5%TE(n=6).Doxorubicin(2.4 mg/kg)was infused immediately after TE injection via the hepatic arteries.After 2 h,the livers were harvested,and doxorubicin concentrations were calculated fluorometrically.The doxorubicin concentrations were compared between TE groups and the control group,and the optimal concentrations within the TE groups were calculated.Statistical analysis was performed using the nonparametric Mann-Whitney U test and Kruskal-Wallis test.P<0.05 were considered statistically significant.RESULTS In the liver,doxorubicin concentrations were 2.06,2.07,2.16 and 1.66 times higher in groups 1 through 4,respectively,and significantly higher in the TE groups than in the control group(all P<0.05).However,there were no significant differences in the mean doxorubicin concentrations between the four TE groups(P=0.642).In the lungs,the mean doxorubicin concentrations were not significantly different between the control and TE groups(P>0.05).CONCLUSION TE infusion into the hepatic arteries significantly increased the doxorubicin concentration approximately twofold but was not different between the TE groups.These findings suggest that TE infusion might be a useful adjuvant treatment of liver cancers.
基金This work was supported by the National Natural Science Foundation of China(Grant No.20337020)the National High-Tech Research and Development Plan(Grant No.2005AA642020)the China Postdoctoral Science Foundation.
文摘A novel triolein-embedded adsorbent for re-moval of persistent organic pollutants (POPs) from water was developed. In this paper, taking dieldrin and endrin as the target pollutants, different factors (temperature, ion strength, pH, and concentration of humic acid) that affect the adsorption rate were investigated. Adsorption isotherms of these two pollutants fitted to Freundlich model well identi-fied the partition mechanism between the adsorbent and water. However, the equilibrium constant of dieldrin was larger than that of endrin due to larger octanol-water coeffi-cient of dieldrin. Adsorption rate of dieldrin increased with the increase of temperature, the decrease of ion strength, pH and concentration of humic acid, whereas adsorption rate of endrin decreased with the increase of temperature and pH. Humic acid firstly increased, and then decreased the adsorp-tion rate of endrin. Ion strength hardly affected the adsorp-tion rate of endrin.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.50578154,20337020)the Hi-Tech Research and Development Program of China(Grant No.2005AA642020).
文摘A novel triolein-embedded activated carbon composite adsorbent was developed. Experiments were car-ried out in areas such as the preparation method, the char-acterization of physicochemical properties, and the adsorp-tion behavior of the composite adsorbent in removing diel-drin from aqueous solution. Results suggested that the novel composite adsorbent was composed of the supporting acti-vated carbon and the surrounding triolein-embedded cellu-lose acetate membrane. The adsorbent was stable in water, for no triolein leakage was detected after soaking the ad-sorbent for five weeks. The adsorbent had good adsorption capability to dieldrin, which was indicated by a residual di-eldrin concentration of 0.204 μg·L?1. The removal efficiency of the composite adsorbent was higher than the traditional activated carbon adsorbent.
基金supported by the National Science and Technology Major Project of China (2009ZX07527-005)the National Natural Science Foundation of China (40801204)the State Key Laboratory of Environmental Aquatic Chemistry of China (10Y03ESPCR)
文摘Passive sampling methods have been widely used for more than 20 years because they can provide information on time-weighted average concentrations(CTWA) of hydrophobic organic contaminants in water.Many factors affect the efficiency and applicability of a passive sampling process.Among others,selection of the sampler design and choice of the supporting membrane material are important factors that determine the performance of passive samplers.In this work,the influences of some critical properties of membranes,such as membrane density,hydrophilicity of the membrane surface,and chemical and mechanical resistances,on the uptake of organic chlorinated pesticides(OCPs) were evaluated.Four membrane materials,namely cellulose acetate(CA),cellulose acetate butyrate(CAB),a cellulose acetate-cellulose triacetate blend(CA-CTA),and cellulose triacetate(CTA),and their triolein-embedded samplers were selected for the study.The four membranes are all hydrophilic;CAB has the highest contact angle and CA has the lowest.CTA is chemically and mechanically resistant,and has better tensile strength than the other membranes have.CAB does not have good chemical resistance and dissolves in hexane/dichloromethane solvent.CA and CTA alone showed lower uptake rates for the selected OCPs than with triolein-embedded passive samplers,indicating that triolein increased the accumulation capacity.Triolein-embedded CA(TECAM),CA-CTA(TECA-CTA),CTA(TECTA),and CAB(TECAB) as passive samplers showed similar uptake patterns;TECTA showed the highest uptake rates and TECAB showed the poorest accumulation.In summary,TECTA gave the best performance among all the tested composite semipermeable membranes.
