[ Objective ] This study was to investigate the preventive effect of cecropin and lysozyme on skin fester disease in Trionyx sinensis. [ Method ] By employing intramuscular injection method, the preventive effect of m...[ Objective ] This study was to investigate the preventive effect of cecropin and lysozyme on skin fester disease in Trionyx sinensis. [ Method ] By employing intramuscular injection method, the preventive effect of mixtures of cecropin （10 mg/kg） and lysozyme（ 10 mg/kg） and of cecropin（6 mg/kg） and lysozyme（ 5 mg/kg） were challenged on Aeromonas encheleia-caused skin fester disease in T. sinensis. The mortality and relative percent survival were used evaluate the preventive effect of different drugs. The serum agglutination titer, cytophagocytosis and bactericidal activity were detected to compare the effect of drugs on the immunity and disease resistance of T. sinensis. [ Result] The residue concentrations of gentamicin and lysozyme in serum were compared to evaluate the side effect of drugs used challenged. The results showed that combined use of cecropin and lysozyme gave the best preventive effect and immunity in T. sinensis, with an extremely significant difference from other treatments （ P 〈 0.01 ）. Analysis of drug residue in serum indicated that lysozyme was completely metabolized 6 days after medication, while gentamicin residue was still observed 12 days later. [ Conclusion] The combined application of cecropin and lysozyme showed better preventive effect than antibiotics in fester disease of T. sinensis. Moreover, no drug residue and side effect were observed in the combined use treatment. This method avoids the side effect of antibiotics, worthy of extension at commercial scale.展开更多
A synthetic biological pond composed of aquatic plants, a microorganism and aquatic animals was applied in the purification of the wastewater discharged from a Tnonyx sinensis breeding greenhouse, and the removal effe...A synthetic biological pond composed of aquatic plants, a microorganism and aquatic animals was applied in the purification of the wastewater discharged from a Tnonyx sinensis breeding greenhouse, and the removal effects of TN, NH4＋ -N, TP and COD in the wastewater were analyzed. The results show that after the breeding wastewater was purified by the synthetic biological pond for about five months, TN, NH~ -N, TP and COD concentration in the breeding wastewater were reduced by 78%, 92%, 81% and 60% respectively, while DO content in the breeding wastewater increased by about four times, and there was no obvious change in water pH; previous yellow or brown and turbid sewage became yel- lowish and slightly turbid, and it had no smell. It is obviously seen that the purification effect of T. sinensis breeding wastewater by the synthetic biological pond composed of aquatic plants, a microorganism and aquatic animals is obvious, and the synthetic biological pond has good promotion and application prospects.展开更多
Based on the analysis of the current situation and existing problems of traditional Chinese soft shelled turtle(Trionyx sinensis)breeding industry,combined with the fact that selenium enriched functional agriculture i...Based on the analysis of the current situation and existing problems of traditional Chinese soft shelled turtle(Trionyx sinensis)breeding industry,combined with the fact that selenium enriched functional agriculture is the future demand of mankind,this paper put forward the necessity of developing selenium enriched Chinese soft shelled turtles and promoting the sustainable development of modern agriculture:the development of selenium-enriched Chinese soft-shelled turtle is the need of the consumer market for food safety,the need to reduce the production cost of soft-shelled turtles and improve the production efficiency of soft-shelled turtles,the need to enrich the Chinese soft-shelled turtle market,and the need to promote the sustainable development of modern agriculture(fishery).展开更多
In the-comparison between two experimental turtle (Trionyx sinensis) groups fed on the feed containing 1 % or 2% Chinese medicinal herb powder and the contrast group to which no Chinese herb was fed,no obvious differe...In the-comparison between two experimental turtle (Trionyx sinensis) groups fed on the feed containing 1 % or 2% Chinese medicinal herb powder and the contrast group to which no Chinese herb was fed,no obvious difference of γ-globulin level in the turtles' serum was found,but the survival rate of the experimental groups was obviously higher than that of the contrast group,and cell immunity of the experimental groups was significantly higher when tested by subcutaneous injection of PHA.Meanwhile,the Chinese herb promoted the growth of the turtles.展开更多
Trionyx sinensis Hemorrhagic Syndrome Virus(TSHSV)is an arterivirus newly discovered in Chinese softshell turtles.Little is known about the effect of antibodies against the virus or the distribution of the virus in di...Trionyx sinensis Hemorrhagic Syndrome Virus(TSHSV)is an arterivirus newly discovered in Chinese softshell turtles.Little is known about the effect of antibodies against the virus or the distribution of the virus in different organs of infected turtles.In this study,a partial protein of TSHSV-HP4 was produced using a prokaryotic expression system,and its polyclonal antibody was generated.The polyclonal antibody was confirmed by western blot and dot enzyme-linked immunosorbent assay(dot-ELISA).The distribution of TSHSV in different organs of T.sinensis was examined by immunohistochemistry(IHC)and the expression of immune-related genes was analyzed using quantitative real-time polymerase chain reaction(qRT-PCR).The results indicated that the recombinant TSHSV-HP4 protein was successfully expressed,and the generated polyclonal antibody showed specific binding to viral particles in the lung tissues of infected turtles.The IHC assay indicated that the virus was highly localized in various cells,including intestinal lymphocytes,enterocytes,kidney epithelial cells,spleen cells,lung macrophages,and cardiomyocytes.The qRT-PCR analysis revealed that TSHSV was detected in all organs tested,including the lungs,liver,kidneys,spleen,and heart.The numbers of viral mRNA copies in lung and heart tissues were significantly higher in the virus-antibody group than in the virus group.The interferonstimulated genes(ISGs),myxovirus resistance protein 2(MX2)and radical S-adenosyl methionine domain containing 2(RSAD2)were highly upregulated in all groups of infected turtles.Antibody-dependent enhancement(ADE)seemed to occur after stimulation by the polyclonal antibody,because significantly greater expression of the two genes was detected in the virus-antibody group than in the virus group.Overall,these results are important in understanding the cell localization of TSHSV and the immune response of infected turtles.展开更多
文摘[ Objective ] This study was to investigate the preventive effect of cecropin and lysozyme on skin fester disease in Trionyx sinensis. [ Method ] By employing intramuscular injection method, the preventive effect of mixtures of cecropin （10 mg/kg） and lysozyme（ 10 mg/kg） and of cecropin（6 mg/kg） and lysozyme（ 5 mg/kg） were challenged on Aeromonas encheleia-caused skin fester disease in T. sinensis. The mortality and relative percent survival were used evaluate the preventive effect of different drugs. The serum agglutination titer, cytophagocytosis and bactericidal activity were detected to compare the effect of drugs on the immunity and disease resistance of T. sinensis. [ Result] The residue concentrations of gentamicin and lysozyme in serum were compared to evaluate the side effect of drugs used challenged. The results showed that combined use of cecropin and lysozyme gave the best preventive effect and immunity in T. sinensis, with an extremely significant difference from other treatments （ P 〈 0.01 ）. Analysis of drug residue in serum indicated that lysozyme was completely metabolized 6 days after medication, while gentamicin residue was still observed 12 days later. [ Conclusion] The combined application of cecropin and lysozyme showed better preventive effect than antibiotics in fester disease of T. sinensis. Moreover, no drug residue and side effect were observed in the combined use treatment. This method avoids the side effect of antibiotics, worthy of extension at commercial scale.
基金Supported by the Project of Public Welfare Application Research from Huzhou Science and Technology Bureau(2013C102070)
文摘A synthetic biological pond composed of aquatic plants, a microorganism and aquatic animals was applied in the purification of the wastewater discharged from a Tnonyx sinensis breeding greenhouse, and the removal effects of TN, NH4＋ -N, TP and COD in the wastewater were analyzed. The results show that after the breeding wastewater was purified by the synthetic biological pond for about five months, TN, NH~ -N, TP and COD concentration in the breeding wastewater were reduced by 78%, 92%, 81% and 60% respectively, while DO content in the breeding wastewater increased by about four times, and there was no obvious change in water pH; previous yellow or brown and turbid sewage became yel- lowish and slightly turbid, and it had no smell. It is obviously seen that the purification effect of T. sinensis breeding wastewater by the synthetic biological pond composed of aquatic plants, a microorganism and aquatic animals is obvious, and the synthetic biological pond has good promotion and application prospects.
基金Supported by Guangxi Science and Technology Program Project of China(GK AD19245169)Yulin City Scientific Research and Technology Development Plan Project(YSK 20204026,2019Cxpt00A4,YSK 20202001,YSK 20204038).
文摘Based on the analysis of the current situation and existing problems of traditional Chinese soft shelled turtle(Trionyx sinensis)breeding industry,combined with the fact that selenium enriched functional agriculture is the future demand of mankind,this paper put forward the necessity of developing selenium enriched Chinese soft shelled turtles and promoting the sustainable development of modern agriculture:the development of selenium-enriched Chinese soft-shelled turtle is the need of the consumer market for food safety,the need to reduce the production cost of soft-shelled turtles and improve the production efficiency of soft-shelled turtles,the need to enrich the Chinese soft-shelled turtle market,and the need to promote the sustainable development of modern agriculture(fishery).
文摘In the-comparison between two experimental turtle (Trionyx sinensis) groups fed on the feed containing 1 % or 2% Chinese medicinal herb powder and the contrast group to which no Chinese herb was fed,no obvious difference of γ-globulin level in the turtles' serum was found,but the survival rate of the experimental groups was obviously higher than that of the contrast group,and cell immunity of the experimental groups was significantly higher when tested by subcutaneous injection of PHA.Meanwhile,the Chinese herb promoted the growth of the turtles.
基金supported by the Zhejiang Provincial Science and Technology Program(Nos.2020YSZX003 and 2020YSZX010)the Zhejiang Provincial Science Exploratory Program(No.2019TSX01),China。
文摘Trionyx sinensis Hemorrhagic Syndrome Virus(TSHSV)is an arterivirus newly discovered in Chinese softshell turtles.Little is known about the effect of antibodies against the virus or the distribution of the virus in different organs of infected turtles.In this study,a partial protein of TSHSV-HP4 was produced using a prokaryotic expression system,and its polyclonal antibody was generated.The polyclonal antibody was confirmed by western blot and dot enzyme-linked immunosorbent assay(dot-ELISA).The distribution of TSHSV in different organs of T.sinensis was examined by immunohistochemistry(IHC)and the expression of immune-related genes was analyzed using quantitative real-time polymerase chain reaction(qRT-PCR).The results indicated that the recombinant TSHSV-HP4 protein was successfully expressed,and the generated polyclonal antibody showed specific binding to viral particles in the lung tissues of infected turtles.The IHC assay indicated that the virus was highly localized in various cells,including intestinal lymphocytes,enterocytes,kidney epithelial cells,spleen cells,lung macrophages,and cardiomyocytes.The qRT-PCR analysis revealed that TSHSV was detected in all organs tested,including the lungs,liver,kidneys,spleen,and heart.The numbers of viral mRNA copies in lung and heart tissues were significantly higher in the virus-antibody group than in the virus group.The interferonstimulated genes(ISGs),myxovirus resistance protein 2(MX2)and radical S-adenosyl methionine domain containing 2(RSAD2)were highly upregulated in all groups of infected turtles.Antibody-dependent enhancement(ADE)seemed to occur after stimulation by the polyclonal antibody,because significantly greater expression of the two genes was detected in the virus-antibody group than in the virus group.Overall,these results are important in understanding the cell localization of TSHSV and the immune response of infected turtles.
