Guinea pig model of asthma was established with the sensitization of the animals with aerosolized ovalbumin. A group of asthmatic guinea pigs were treated with tripholide. It was found that the airway responsiveness t...Guinea pig model of asthma was established with the sensitization of the animals with aerosolized ovalbumin. A group of asthmatic guinea pigs were treated with tripholide. It was found that the airway responsiveness to histamine was markedly higher in the asthmatic group than in the control. The total cell count, absolute eosinophil count, percentage of hypodense eosinophils and the level of eosinophil viability enhancing activity in the bronchoalveolar lavage fluid (BALF) were significantly higher in the asthmatic group than in the control. The percentage of hypodense eosinophils and absolute eosinophil count were in close correlation with airway responsiveness (r= -0. 76 and - 0. 88, P <0. 05 and 0. 01 respectively). Treatment of the asthmatic guinea pigs with tripholide (8 μg/kg, given orally for 7 d) significantly reduced the airway responsiveness and decreased the percentage of hypodense eosinophils and the level of eosinophil viability enhancing activity but eosinophilia in BALF was not affected. It was concluded that tripholide exerts its effects to decrease airway responsiveness in asthma through its action to reduce active hypodense eosinophils.展开更多
文摘Guinea pig model of asthma was established with the sensitization of the animals with aerosolized ovalbumin. A group of asthmatic guinea pigs were treated with tripholide. It was found that the airway responsiveness to histamine was markedly higher in the asthmatic group than in the control. The total cell count, absolute eosinophil count, percentage of hypodense eosinophils and the level of eosinophil viability enhancing activity in the bronchoalveolar lavage fluid (BALF) were significantly higher in the asthmatic group than in the control. The percentage of hypodense eosinophils and absolute eosinophil count were in close correlation with airway responsiveness (r= -0. 76 and - 0. 88, P <0. 05 and 0. 01 respectively). Treatment of the asthmatic guinea pigs with tripholide (8 μg/kg, given orally for 7 d) significantly reduced the airway responsiveness and decreased the percentage of hypodense eosinophils and the level of eosinophil viability enhancing activity but eosinophilia in BALF was not affected. It was concluded that tripholide exerts its effects to decrease airway responsiveness in asthma through its action to reduce active hypodense eosinophils.
