Observations on Therapeutic Effects of Huangdan Decoction and Tripterygium Wilfordii Compound Tablet on Membranous Glomerulon

The model of membranous glomerulonephritis(MGN)in rats was successfully established using self-made cationic bovine serum albumin(C-BSA)and treated with Huangdan Decoction (HDD) and Tripterygium Wilfordii Co.tablet(TW).Results indicated that the levels of urinary protein,blood urea nitrogen(BUN)and serum creatinine(Scr) in treated groups(groups A,B and C)were significantly decreased as compared with the control group(group D)(PM0.01).By light and electron microscope and immunofluorescent technique,the damage to kidney in groups A,B and C was found much milder than that in group D with lesion in group A being slightest.These findings suggest that HDD and TW may alleviate the pathological lesions of MGN,prevent or retard its progression,and have remarkable therapeutic effects on MGN.

Effect of Tripterygium Vilfordii on Adrenal Cortex in Rat with Adjuvant Arthritis

This paper reports the effects of Tripterygium Wilfordii (TW) on adrenal cortex in rats with adjuvant artbritis. Forty rats were divided into 5 groups. Adjuvant arthritis (AA) models were made with complete freund's adjuvant (CFA) in groups Ⅰ-Ⅳ. Each of which was treated with sodium carboxyl methyl cellulose, TW, prednisone and cyclophosphamide respecrively.The untreated rats allocated to group,V served as normal. controls. The swelling of AA markedly subsided in groups Ⅱ,Ⅲ and Ⅳ as compasred with group I (P<0.01). whereas no significant differences were noted among groups Ⅱ, Ⅲ and Ⅳ(P>0.05).The obviously increased plasma corticosterone levels and decreased adrenal ascorbic acid levels were observed in group Ⅱ,whereas decreased plasma corticosterone levels and inereased adrenal ascorbic acid levels were noted in group Ⅲ.There was a striking comtrast between groups Ⅱand Ⅲ. The morphological changes of adrenal glands under light microseope revealed hypertrophic adrenal cortices in group Ⅱ,and atrophic adrenal cortices in group Ⅲ.The above findings snggest that that the effect promoting production of corticosterods may be one of mechanism by which TW can effectively treating autoimmune diseases.

雷公藤多苷联合小剂量激素治疗特发性膜性肾病前瞻性对照研究

目的:特发性膜性肾病(IMN)是成人肾病综合征的常见原因之一,但是膜性肾病的治疗仍是临床上的一个难题。本文前瞻性地观察了单用雷公藤多苷(TW)和TW联合小剂量泼尼松(TW+P)治疗IMN的临床疗效和安全性。方法:经肾活检并结合临床诊断为IMN患者,尿蛋白>3.5 g/24h。随机分为2组。TW组:TW120 mg/d口服,至3个月完全缓解者,减量为维持剂量(60 mg/d),持续至12个月。3个月时部分缓解或无效者,TW 120 mg/d可延长至6个月,再减量为60 mg/d维持至12个月。TW+P组:TW用药同上,同时服用泼尼松30mg/d,8周后逐渐减量(每2周隔日减5 mg),10 mg隔/日维持至12个月。结果:共84例患者入组,其中TW+P治疗组43例,TW治疗组41例。治疗3个月,TW+P组3例(6.98%)完全缓解,29例(67.4%)部分缓解,有效率为74.4%;TW组无一例完全缓解,21例(51.2%)患者部分缓解。有效率为51.2%。治疗6个月,TW+P组13例(30.2%)完全缓解,21例(48.8%)部分缓解,有效率为79.1%;TW组仍为21例(51.2%)部分缓解,无一例完全缓解,有效率为51.2%。治疗12个月,TW+P组16例(37.2%)完全缓解,17例(39.5%)部分缓解,有效率为76.7%,而TW组2例(4.88%)达到完全缓解,16例(39%)部分缓解,有效率为43.9%。TW+P组有效率和完全缓解率均明显高于TW组。不良反应的发生率两组之间无明显差异。起病年龄轻和小管间质病变较轻的IMN患者对治疗反应较好。治疗过程中两组患者血清肌酐均保持稳定。结论:TW能有效减少膜型肾病患者蛋白尿。TW+P治疗的疗效明显优于单用TW。患者耐受性好,不良反应少,是治疗IMN的有效方法。

雷公藤多苷对移植肾长期存活率的影响

目的:探讨雷公藤多苷(TW)作为免疫抑制剂对肾移植患者长期存活率的疗效及副作用。方法:104例患者在肾移植术后采用TW+泼尼松(Pred)+环孢霉素(CsA)+硫唑嘌呤(AZa)免疫抑制剂治疗,48例患者在肾移植术后采用Pred+CsA+骁悉(MMF)免疫抑制剂治疗,就以下方面对两组患者进行观察比较:(1)术后5年内发生排斥反应及临界改变情况;(2)外周血白细胞下降、肝功能异常的发生率;(3)严重感染情况;(4)出现尿蛋白情况;(5)术后5年内肾功能变化情况及移植肾5年存活率。结果:5年内AZa+TW组急性排斥反应及临界改变的发生率较MMF组低,分别为11.5%、16.7%和4.8%、6.3%(P>0.05);GPT高于正常的发生率分别为7.7%、16.6%(P>0.05);外周血白细胞低于正常的发生率分别为0.96%、18.8%(P<0.01);严重感染的发生率分别为1.9%、18.8%(P<0.05),5年内出现蛋白尿的患者例数分别为17.3%、29.2%(P>0.05),两组移植肾5年存活率相似,分别为89.6%、85.4%(P>0.05)。结论:在肾移植术后应用CsA+Pred+AZa+TW免疫抑制方案是可行的,既可以使肾移植术后急性排斥反应减少,同时还能减少蛋白尿及慢性排斥反应的发生率下降,为国内提高移植肾长期存活率提供了一条新的途径。

抗生育剂量雷公藤对小鼠腹腔巨噬细胞TNF-α生成的影响

观察两组不同低剂量雷公藤糖浆对雄性小鼠生殖功能和腹腔巨噬细胞TNF-α生成能力的影响,以合笼试验为指标观察到两组剂量雷公藤糖浆均有抗雄性生育作用,同时观察到对小鼠腹腔巨噬细胞TNF-α生成能力无明显影响(P>0.05)。上述结果提示雷公藤在有效抗生育作用剂量下对小鼠腹腔巨噬细胞TNF-α生成能力无明显影响。