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DF-1细胞二维电泳方法的建立 被引量:3
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作者 范忠军 胡序明 +2 位作者 郁川 秦爱建 王欢莉 《河南科技大学学报(自然科学版)》 CAS 北大核心 2015年第2期83-87,7,共5页
为了建立和优化DF-1细胞二维电泳方法,对5种细胞裂解液进行了比较;同时,还对提取的蛋白在不同的等电聚焦条件、水化条件、蛋白上样量、SDS-聚丙烯酰胺凝胶浓度条件下的二维电泳图谱进行分析比较,并用PDQuest软件分析优化选择。建立了DF-... 为了建立和优化DF-1细胞二维电泳方法,对5种细胞裂解液进行了比较;同时,还对提取的蛋白在不同的等电聚焦条件、水化条件、蛋白上样量、SDS-聚丙烯酰胺凝胶浓度条件下的二维电泳图谱进行分析比较,并用PDQuest软件分析优化选择。建立了DF-1细胞的二维电泳方法,获得分辨率和重复性均可满足分析要求的DF-1细胞的二维电泳图谱。 展开更多
关键词 DF-1 细胞 二维电泳 裂解液 蛋白质组学
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Progress in research of GaN-based LEDs fabricated on SiC substrate 被引量:1
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作者 徐化勇 陈秀芳 +4 位作者 彭燕 徐明升 沈燕 胡小波 徐现刚 《Chinese Physics B》 SCIE EI CAS CSCD 2015年第6期31-38,共8页
The influence of buffer layer growth conditions on the crystal quality and residual stress of GaN film grown on silicon carbide substrate is investigated. It is found that the A1GaN nucleation layer with high growth t... The influence of buffer layer growth conditions on the crystal quality and residual stress of GaN film grown on silicon carbide substrate is investigated. It is found that the A1GaN nucleation layer with high growth temperature can efficiently decrease the dislocation density and stress of the GaN film compared with A1N buffer layer. To increase the light extraction efficiency of GaN-based LEDs on SiC substrate, flip-chip structure and thin film flip-chip structure were designed and optimized. The fabricated blue LED had a maximum wall-plug efficiency of 72% at 80 mA. At 350 mA, the output power, the Vf, the dominant wavelength, and the wall-plug efficiency of the blue LED were 644 roW, 2.95 V, 460 nm, and 63%, respectively. 展开更多
关键词 SIC GAN A1GaN buffer light emitting diode flip chip light extraction efficiency
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