With rutin standard sample as the comparison, flavonoid extraction rate as an indicator, UV-2450 ultraviolet visible light spectrophotometer to measure flavonoid content in planted Trollius chinensis through orthogona...With rutin standard sample as the comparison, flavonoid extraction rate as an indicator, UV-2450 ultraviolet visible light spectrophotometer to measure flavonoid content in planted Trollius chinensis through orthogonal experiment, this experiment optimizes process condition of flavonoid in Trollius chinensis through water extraction and ethanol extraction. The result shows that flavonoid extraction rate of Trollius chinensis through ethanol extraction method is obviously higher than water extraction, and the optimal extraction process condition is： ethanol concentration is 75%, extraction time is 90min, extraction times are 3, and fluid material ratio is 20：1.展开更多
It takes flavonoid extraction rate as indicator and adopts the method of orthogonal experiment to study process of total flavonoid in Trollius chinensis through ultrasonic wave assisted extraction, microwave assisted ...It takes flavonoid extraction rate as indicator and adopts the method of orthogonal experiment to study process of total flavonoid in Trollius chinensis through ultrasonic wave assisted extraction, microwave assisted extraction and backflow extraction, as well as optimization of process parameter. The result indicates that in terms of extraction efficiency, microwave extraction method is better than ultrasonic radiation extraction method, which is better than backflow extraction method. Optimal process parameter through backflow extraction is： backflow temperature 60℃, fluid material ratio 1：20, ethanol density 65%, backflow time 60min; optimal process parameter through ultrasonic wave assisted extraction is： ultrasonic radiation temperature 70℃, fluid material ratio 1：20, ethanol density 65%, ultrasonic wave radiation time 45rain; optimal process parameter of microwave assisted extraction is： microwave radiation temperature 60℃, fluid material ratio 1：20, ethanol density 75%, microwave radiation time 45min.展开更多
Objective To determine the optimum extraction method and condition of poly-saccharides fromTrollius chinensis (TCP).Methods With the yield of TCP as index and water as solvent,reflux extraction,ultrasonic extraction a...Objective To determine the optimum extraction method and condition of poly-saccharides fromTrollius chinensis (TCP).Methods With the yield of TCP as index and water as solvent,reflux extraction,ultrasonic extraction and supercritical CO_2 extraction were employed to determine the efficient extraction method of TCP.Moreover,the conditions of reflux extraction were optimized by single factor test and orthogonal test.The content of polysaccharide was measured with phenol-sulfuric acid method.Results The data displayed that the crude polysaccharide(CPS)yield of regular reflux extraction was(11.7±0.12)%,superior to ultrasonic extraction and supercritical CO_2 extraction.The optimum conditions of reflux extraction were as follows:extraction temperature of 95℃,extraction time of 3hand the liquid-solid ratio of 20∶1.Under these conditions,the polysaccharide yield was (1.45±0.03)%,which was well in close agreement with the predicted value.Conclusion Regular reflux extraction was regarded as the optimal extraction method of TCP.The optimum conditions of reflux extraction were as follows:extraction temperature of 95℃,extraction time of 3hand the liquid-solid ratio of 20∶1.展开更多
文摘With rutin standard sample as the comparison, flavonoid extraction rate as an indicator, UV-2450 ultraviolet visible light spectrophotometer to measure flavonoid content in planted Trollius chinensis through orthogonal experiment, this experiment optimizes process condition of flavonoid in Trollius chinensis through water extraction and ethanol extraction. The result shows that flavonoid extraction rate of Trollius chinensis through ethanol extraction method is obviously higher than water extraction, and the optimal extraction process condition is： ethanol concentration is 75%, extraction time is 90min, extraction times are 3, and fluid material ratio is 20：1.
文摘It takes flavonoid extraction rate as indicator and adopts the method of orthogonal experiment to study process of total flavonoid in Trollius chinensis through ultrasonic wave assisted extraction, microwave assisted extraction and backflow extraction, as well as optimization of process parameter. The result indicates that in terms of extraction efficiency, microwave extraction method is better than ultrasonic radiation extraction method, which is better than backflow extraction method. Optimal process parameter through backflow extraction is： backflow temperature 60℃, fluid material ratio 1：20, ethanol density 65%, backflow time 60min; optimal process parameter through ultrasonic wave assisted extraction is： ultrasonic radiation temperature 70℃, fluid material ratio 1：20, ethanol density 65%, ultrasonic wave radiation time 45rain; optimal process parameter of microwave assisted extraction is： microwave radiation temperature 60℃, fluid material ratio 1：20, ethanol density 75%, microwave radiation time 45min.
基金Major Scientific Projects of Hebei North University(No.ZD1314)Innovation Fund Project of Graduate of Hebei Province(2015-203and 2016-288)
文摘Objective To determine the optimum extraction method and condition of poly-saccharides fromTrollius chinensis (TCP).Methods With the yield of TCP as index and water as solvent,reflux extraction,ultrasonic extraction and supercritical CO_2 extraction were employed to determine the efficient extraction method of TCP.Moreover,the conditions of reflux extraction were optimized by single factor test and orthogonal test.The content of polysaccharide was measured with phenol-sulfuric acid method.Results The data displayed that the crude polysaccharide(CPS)yield of regular reflux extraction was(11.7±0.12)%,superior to ultrasonic extraction and supercritical CO_2 extraction.The optimum conditions of reflux extraction were as follows:extraction temperature of 95℃,extraction time of 3hand the liquid-solid ratio of 20∶1.Under these conditions,the polysaccharide yield was (1.45±0.03)%,which was well in close agreement with the predicted value.Conclusion Regular reflux extraction was regarded as the optimal extraction method of TCP.The optimum conditions of reflux extraction were as follows:extraction temperature of 95℃,extraction time of 3hand the liquid-solid ratio of 20∶1.
