Background:The role of TROVE domain family member 2(TROVE2)has been well-demonstrated in autoimmune diseases;however,its involvement in liver cancer remains unclear.Therefore,this study aimed to explore the biological...Background:The role of TROVE domain family member 2(TROVE2)has been well-demonstrated in autoimmune diseases;however,its involvement in liver cancer remains unclear.Therefore,this study aimed to explore the biological function and clinical significance of TROVE2 in hepatocellular carcinoma(HCC).Methods:The expression level of TROVE2 was analyzed in HCC and paired adjacent tissue samples using real-time reverse transcription-quantitative polymerase chain reaction.The impact of TROVE2 on migration and invasion in HCC cells was analyzed through Transwell assays and Western blotting.High-throughput transcriptome sequencing and bioinformatics analyses were performed to identify downstream target genes.Back-complementation experiments were employed to verify the influence of downstream proteins on TROVE2-induced invasion and migration of HCC cells.Results:TROVE2 exhibited significant overexpression in liver cancer tissue,correlating with shorter overall survival.Overexpression of TROVE2 facilitated the invasion,metastasis,and epithelial-mesenchymal transition(EMT)process of HCC cells,whereas TROVE2 knockdown restrained migration,invasion,and EMT in these cells.Transcriptome sequencing and bioinformatics analysis identified heparanase(HPSE)as a downstreamtarget protein of TROVE2.Subsequent back-complementation experiments provided evidence that HPSE overexpression promoted TROVE2-mediated prometastasis effects.Moreover,the study revealed that TROVE2 was capable of regulating the EMT pathway through GSK-3βphosphorylation.Conclusions:TROVE2 facilitated the invasion,migration,and EMT process ofHCC cells through phosphorylation of the HPSE/GSK-3βaxis,indicating its significance as an important protein in tumor progression.展开更多
MAOTIAN is a cap-shaped mountain 52 km southeast of Kunming, the capital of Yunnan Province. It came to public attention in the 1980s after a big trove of fossils dating back 530 million years was discovered there. Th...MAOTIAN is a cap-shaped mountain 52 km southeast of Kunming, the capital of Yunnan Province. It came to public attention in the 1980s after a big trove of fossils dating back 530 million years was discovered there. This was called one of the biggest scientific discoveries of the 20th century. As Maotian is located in Chengjiang County, the fossil site is named after the county.展开更多
Beijing steps up efforts to build green lungs with mini parks Zhu Jianguo showed up at the community park unfailingly at 6 a.m., accompanied by his little dog Zhongqiu. He is a regular at the park and these little jau...Beijing steps up efforts to build green lungs with mini parks Zhu Jianguo showed up at the community park unfailingly at 6 a.m., accompanied by his little dog Zhongqiu. He is a regular at the park and these little jaunts are his lifeline.Ten years ago, Zhu moved to Beijing from Tianshui, northwest China’s Gansu Province. He lived with his daughter but felt lonely, because she was often away. This lasted until a mini park opened nearby. The 870-square-meter Xishiku Pocket Park opened in 2018.展开更多
The Palace Museum inventories its collection For the first time in history, curators of the Palace Museum have finally inven- toried the museum’s vast collection. After seven years of painstaking work, the museum fin...The Palace Museum inventories its collection For the first time in history, curators of the Palace Museum have finally inven- toried the museum’s vast collection. After seven years of painstaking work, the museum finally released the results of its first comprehensive inventory on January 26, showing that the museum houses 1,807,558 items.展开更多
The Phase II Project of the National Library of China features not only hi-tech but also reader-friendly services September 9, 2008 marked a mile-stone for the 99-year-old
基金the Natural Science Foundation of Fujian Province(2021 J01539,2023 J011467)Scientific Foundation of the Fuzhou Health Commission(2021-S-wq21,2021-S-wp1).
文摘Background:The role of TROVE domain family member 2(TROVE2)has been well-demonstrated in autoimmune diseases;however,its involvement in liver cancer remains unclear.Therefore,this study aimed to explore the biological function and clinical significance of TROVE2 in hepatocellular carcinoma(HCC).Methods:The expression level of TROVE2 was analyzed in HCC and paired adjacent tissue samples using real-time reverse transcription-quantitative polymerase chain reaction.The impact of TROVE2 on migration and invasion in HCC cells was analyzed through Transwell assays and Western blotting.High-throughput transcriptome sequencing and bioinformatics analyses were performed to identify downstream target genes.Back-complementation experiments were employed to verify the influence of downstream proteins on TROVE2-induced invasion and migration of HCC cells.Results:TROVE2 exhibited significant overexpression in liver cancer tissue,correlating with shorter overall survival.Overexpression of TROVE2 facilitated the invasion,metastasis,and epithelial-mesenchymal transition(EMT)process of HCC cells,whereas TROVE2 knockdown restrained migration,invasion,and EMT in these cells.Transcriptome sequencing and bioinformatics analysis identified heparanase(HPSE)as a downstreamtarget protein of TROVE2.Subsequent back-complementation experiments provided evidence that HPSE overexpression promoted TROVE2-mediated prometastasis effects.Moreover,the study revealed that TROVE2 was capable of regulating the EMT pathway through GSK-3βphosphorylation.Conclusions:TROVE2 facilitated the invasion,migration,and EMT process ofHCC cells through phosphorylation of the HPSE/GSK-3βaxis,indicating its significance as an important protein in tumor progression.
文摘MAOTIAN is a cap-shaped mountain 52 km southeast of Kunming, the capital of Yunnan Province. It came to public attention in the 1980s after a big trove of fossils dating back 530 million years was discovered there. This was called one of the biggest scientific discoveries of the 20th century. As Maotian is located in Chengjiang County, the fossil site is named after the county.
文摘Beijing steps up efforts to build green lungs with mini parks Zhu Jianguo showed up at the community park unfailingly at 6 a.m., accompanied by his little dog Zhongqiu. He is a regular at the park and these little jaunts are his lifeline.Ten years ago, Zhu moved to Beijing from Tianshui, northwest China’s Gansu Province. He lived with his daughter but felt lonely, because she was often away. This lasted until a mini park opened nearby. The 870-square-meter Xishiku Pocket Park opened in 2018.
文摘The Palace Museum inventories its collection For the first time in history, curators of the Palace Museum have finally inven- toried the museum’s vast collection. After seven years of painstaking work, the museum finally released the results of its first comprehensive inventory on January 26, showing that the museum houses 1,807,558 items.
文摘The Phase II Project of the National Library of China features not only hi-tech but also reader-friendly services September 9, 2008 marked a mile-stone for the 99-year-old
