目的 探讨颅脑外伤患者围手术期硫氧还蛋白1(Trx1)、神经生长因子(NGF)、高敏C反应蛋白(hs-CRP)动态变化对预后的预测效能。方法 选取2019年3月—2021年5月空军军医大学第二附属医院收治的105例颅脑外伤患者,根据预后不同分为预后不良组...目的 探讨颅脑外伤患者围手术期硫氧还蛋白1(Trx1)、神经生长因子(NGF)、高敏C反应蛋白(hs-CRP)动态变化对预后的预测效能。方法 选取2019年3月—2021年5月空军军医大学第二附属医院收治的105例颅脑外伤患者,根据预后不同分为预后不良组(28例)、良好组(77例),比较两组临床资料、术前、术后3 d、术后7 d Trx1、NGF、hs-CRP水平,应用Pearson及偏相关性分析各指标与格拉斯哥预后量表(GOS)评分关系,采用受试者工作特征曲线(ROC)及ROC下面积(AUC)分析术后7 d Trx1、NGF、hs-CRP预测预后效能。结果 不良组入院时APACHEⅡ评分与良好组比较,差异有统计学意义(P<0.05);不良组术后3 d、术后7 d Trx1、NGF低于良好组,hs-CRP高于良好组(P<0.05);术后3 d、术后7 d Trx1、NGF与GOS评分呈正相关,术后3 d、术后7 d hs-CRP与GOS评分呈负相关(P<0.05);将混杂因素控制后,术后7 d Trx1、NGF、hs-CRP仍与GOS评分相关(P<0.05);术后7 d Trx1、NGF联合hs-CRP预测预后的AUC为0.890,大于Trx1的0.771、NGF的0.753,hs-CRP的0.858,预测敏感度为71.43%,特异度为90.91%。结论 颅脑外伤患者术后Trx1、NGF降低及hs-CRP升高与预后不良有关,联合检测三者水平有望成为预测预后的一个可靠方案,为临床干预、决策、监护等提供参考。展开更多
BACKGROUND Identifying biomarkers for the risk of developing degenerative processes linked to aging and colorectal cancer(CRC) onset that could improve clinical strategies.AIM To determine valid targets and a predicti...BACKGROUND Identifying biomarkers for the risk of developing degenerative processes linked to aging and colorectal cancer(CRC) onset that could improve clinical strategies.AIM To determine valid targets and a predictive biomarker's system of chronicization of inflammation for cancer treatment.METHODS A group of 147 CRC patients was studied. Clinical diagnosis was confirmed histopathologically, and patients were sub-typed using the pathological tumornode-metastasis classification. Thirteen colon adenoma patients and 219 healthy subjects were also studied. A system biology study on Thioredoxin1/CD30 redox-immune systems(Trx1/CD30), T helper cytokines and polymorphisms of killer immunoglobulin-like receptors, FcγRIIa-131 H/R and FcγRIIIa-158 V/F was carried out. Enzyme-linked immunosorbent assay was performed to analyze sera.Genetic study was executed by polymerase chain reaction sequence-specific primers and sequence-based typing method. Statistical analysis was performed by using the "Statgraphics software systems".RESULTS We found a positive increase between Trx1/RTrx1 levels and sCD30 level and increased age. With respect to the gender relationships, there were distinct differences. Females showed a primary relationship between transforming growth factor beta(TGFβ) with Trx1, whereas males had one with TGFβ and RTrx1. Trx1/CD30 controls the redox immune homeostasis, and an imbalance in the relationship between the Trx1/RTrx1 and sCD30 levels is linked to the onset and progression of tumor. This event happens through different gender-specific cytokine pathways. Our study demonstrated that the serum levels ofTrx1/RTrx1, TGFβ/interleukin(IL)6 and TGFβ/IL4 combinations and the sCD30,IFNγ and IL2 combination constitute a predictive gender specific biomarker system. This is relevant for clinical screening to detect the risk of the potential development or progression of a tumor.CONCLUSION Oxidative stress on Trx1/CD30 is a trigger of cancer disease, and the selected oxidation and immune products are a biomarker system for aging and cancer.展开更多
Heficobacterpylori infection is related to the development of gastric diseases. Our previous studies showed that high thioredoxin-1 （Trxl） expression in H. pylori can promote gastric carcinogenesis. To explore the u...Heficobacterpylori infection is related to the development of gastric diseases. Our previous studies showed that high thioredoxin-1 （Trxl） expression in H. pylori can promote gastric carcinogenesis. To explore the underlying molecular mechanisms, we performed an isobaric tags for relative and absolute quantitation （iTRAQ）-based quantitative proteomic analysis of stomach tissues from Mongolian gerbil infected with H. pylori expressing high and low Trxl Differences in the profiles of the expressed proteins were analyzed by bioinformatics and verified using Western blot analysis. We found three candidate proteins, 14-3-3α/β, glutathione-S-transferase （GST）, and heat shock protein 70 （HSP70）, in high Trxl tissues compared with low Trxl tissues and concluded that cellular stress and redox activity- related proteins were involved in the pathogenesis of gastric cancer associated with H. pylori Trxl.展开更多
文摘目的 探讨颅脑外伤患者围手术期硫氧还蛋白1(Trx1)、神经生长因子(NGF)、高敏C反应蛋白(hs-CRP)动态变化对预后的预测效能。方法 选取2019年3月—2021年5月空军军医大学第二附属医院收治的105例颅脑外伤患者,根据预后不同分为预后不良组(28例)、良好组(77例),比较两组临床资料、术前、术后3 d、术后7 d Trx1、NGF、hs-CRP水平,应用Pearson及偏相关性分析各指标与格拉斯哥预后量表(GOS)评分关系,采用受试者工作特征曲线(ROC)及ROC下面积(AUC)分析术后7 d Trx1、NGF、hs-CRP预测预后效能。结果 不良组入院时APACHEⅡ评分与良好组比较,差异有统计学意义(P<0.05);不良组术后3 d、术后7 d Trx1、NGF低于良好组,hs-CRP高于良好组(P<0.05);术后3 d、术后7 d Trx1、NGF与GOS评分呈正相关,术后3 d、术后7 d hs-CRP与GOS评分呈负相关(P<0.05);将混杂因素控制后,术后7 d Trx1、NGF、hs-CRP仍与GOS评分相关(P<0.05);术后7 d Trx1、NGF联合hs-CRP预测预后的AUC为0.890,大于Trx1的0.771、NGF的0.753,hs-CRP的0.858,预测敏感度为71.43%,特异度为90.91%。结论 颅脑外伤患者术后Trx1、NGF降低及hs-CRP升高与预后不良有关,联合检测三者水平有望成为预测预后的一个可靠方案,为临床干预、决策、监护等提供参考。
文摘BACKGROUND Identifying biomarkers for the risk of developing degenerative processes linked to aging and colorectal cancer(CRC) onset that could improve clinical strategies.AIM To determine valid targets and a predictive biomarker's system of chronicization of inflammation for cancer treatment.METHODS A group of 147 CRC patients was studied. Clinical diagnosis was confirmed histopathologically, and patients were sub-typed using the pathological tumornode-metastasis classification. Thirteen colon adenoma patients and 219 healthy subjects were also studied. A system biology study on Thioredoxin1/CD30 redox-immune systems(Trx1/CD30), T helper cytokines and polymorphisms of killer immunoglobulin-like receptors, FcγRIIa-131 H/R and FcγRIIIa-158 V/F was carried out. Enzyme-linked immunosorbent assay was performed to analyze sera.Genetic study was executed by polymerase chain reaction sequence-specific primers and sequence-based typing method. Statistical analysis was performed by using the "Statgraphics software systems".RESULTS We found a positive increase between Trx1/RTrx1 levels and sCD30 level and increased age. With respect to the gender relationships, there were distinct differences. Females showed a primary relationship between transforming growth factor beta(TGFβ) with Trx1, whereas males had one with TGFβ and RTrx1. Trx1/CD30 controls the redox immune homeostasis, and an imbalance in the relationship between the Trx1/RTrx1 and sCD30 levels is linked to the onset and progression of tumor. This event happens through different gender-specific cytokine pathways. Our study demonstrated that the serum levels ofTrx1/RTrx1, TGFβ/interleukin(IL)6 and TGFβ/IL4 combinations and the sCD30,IFNγ and IL2 combination constitute a predictive gender specific biomarker system. This is relevant for clinical screening to detect the risk of the potential development or progression of a tumor.CONCLUSION Oxidative stress on Trx1/CD30 is a trigger of cancer disease, and the selected oxidation and immune products are a biomarker system for aging and cancer.
基金Project supported by the Key Laboratory for Helicobacter pylori Infection and Upper Gastrointestinal Diseases,Beijing Key Laboratory(No.BZ0371)the National Natural Science Foundation of China(Nos.81700496,81270475,and 30770980)
文摘Heficobacterpylori infection is related to the development of gastric diseases. Our previous studies showed that high thioredoxin-1 （Trxl） expression in H. pylori can promote gastric carcinogenesis. To explore the underlying molecular mechanisms, we performed an isobaric tags for relative and absolute quantitation （iTRAQ）-based quantitative proteomic analysis of stomach tissues from Mongolian gerbil infected with H. pylori expressing high and low Trxl Differences in the profiles of the expressed proteins were analyzed by bioinformatics and verified using Western blot analysis. We found three candidate proteins, 14-3-3α/β, glutathione-S-transferase （GST）, and heat shock protein 70 （HSP70）, in high Trxl tissues compared with low Trxl tissues and concluded that cellular stress and redox activity- related proteins were involved in the pathogenesis of gastric cancer associated with H. pylori Trxl.
