Tu-Xian decoction(TXD),a traditional Chinese medicine(TCM)formula,has been frequently administered to manage diabetic cognitive impairment(DCI).Despite its widespread use,the mechanisms underlying TXD’s protective ef...Tu-Xian decoction(TXD),a traditional Chinese medicine(TCM)formula,has been frequently administered to manage diabetic cognitive impairment(DCI).Despite its widespread use,the mechanisms underlying TXD’s protective effects on DCI have yet to be fully elucidated.As a significant regulator in neurodegenerative conditions,death-associated protein kinase-1(DAPK-1)serves as a focus for understanding the action of TXD.This study was designed to whether TXD mediates its beneficial outcomes by inhibiting DAPK-1.To this end,a diabetic model was established using Sprague-Dawley(SD)rats through a high-fat,high-sugar(HFHS)diet regimen,followed by streptozotocin(STZ)injection.The experimental cohort was stratified into six groups:Control,Diabetic,TC-DAPK6,high-dose TXD,medium-dose TXD,and low-dose TXD groups.Following a 12-week treatment period,various assessments—including blood glucose levels,body weight measurements,Morris water maze(MWM)testing for cognitive function,brain magnetic resonance imaging(MRI),and histological analyses using hematoxylin-eosin(H&E),and Nissl staining—were conducted.Protein expression in the hippocampus was quantified through Western blotting analysis.The results revealed that TXD significantly improved spatial learning and memory abilities,and preserved hippocampal structure in diabetic rats.Importantly,TXD administration led to a down-regulation of proteins indicative of neurological damage and suppressed DAPK-1 activity within the hippocampal region.These results underscore TXD's potential in mitigating DCI via DAPK-1 inhibition,positioning it as a viable therapeutic candidate for addressing this condition.Further investigation into TXD's molecular mechanisms may elucidate new pathways for the treatment of DCI.展开更多
甲午中日海战以筹备近十年的北洋水师全军覆没而告终,同时也标志着三十余年洋务运动的最终失败。面对危急的时局,时人纷纷思考“器物”之外,中国还缺少什么?在与之直接关联的海防领域,除了坚船利炮,海防思想也不可忽视。晚清著名翻译家...甲午中日海战以筹备近十年的北洋水师全军覆没而告终,同时也标志着三十余年洋务运动的最终失败。面对危急的时局,时人纷纷思考“器物”之外,中国还缺少什么?在与之直接关联的海防领域,除了坚船利炮,海防思想也不可忽视。晚清著名翻译家陈寿彭选取英国海道测量局①所编纂《中国海指南》②(The China Sea Directory)第三版第三卷中有关中国沿海的部分内容,将其翻译成《新译中国江海险要图志》(下文简称《图志》)。展开更多
基金This work was supported by the National Natural Science Foundation of China(No.82074404).
文摘Tu-Xian decoction(TXD),a traditional Chinese medicine(TCM)formula,has been frequently administered to manage diabetic cognitive impairment(DCI).Despite its widespread use,the mechanisms underlying TXD’s protective effects on DCI have yet to be fully elucidated.As a significant regulator in neurodegenerative conditions,death-associated protein kinase-1(DAPK-1)serves as a focus for understanding the action of TXD.This study was designed to whether TXD mediates its beneficial outcomes by inhibiting DAPK-1.To this end,a diabetic model was established using Sprague-Dawley(SD)rats through a high-fat,high-sugar(HFHS)diet regimen,followed by streptozotocin(STZ)injection.The experimental cohort was stratified into six groups:Control,Diabetic,TC-DAPK6,high-dose TXD,medium-dose TXD,and low-dose TXD groups.Following a 12-week treatment period,various assessments—including blood glucose levels,body weight measurements,Morris water maze(MWM)testing for cognitive function,brain magnetic resonance imaging(MRI),and histological analyses using hematoxylin-eosin(H&E),and Nissl staining—were conducted.Protein expression in the hippocampus was quantified through Western blotting analysis.The results revealed that TXD significantly improved spatial learning and memory abilities,and preserved hippocampal structure in diabetic rats.Importantly,TXD administration led to a down-regulation of proteins indicative of neurological damage and suppressed DAPK-1 activity within the hippocampal region.These results underscore TXD's potential in mitigating DCI via DAPK-1 inhibition,positioning it as a viable therapeutic candidate for addressing this condition.Further investigation into TXD's molecular mechanisms may elucidate new pathways for the treatment of DCI.
文摘甲午中日海战以筹备近十年的北洋水师全军覆没而告终,同时也标志着三十余年洋务运动的最终失败。面对危急的时局,时人纷纷思考“器物”之外,中国还缺少什么?在与之直接关联的海防领域,除了坚船利炮,海防思想也不可忽视。晚清著名翻译家陈寿彭选取英国海道测量局①所编纂《中国海指南》②(The China Sea Directory)第三版第三卷中有关中国沿海的部分内容,将其翻译成《新译中国江海险要图志》(下文简称《图志》)。
