The Expression and Change of the β_3 Integrin Subunit and Fibronectin in Normal Human Oviductal Tissue and Tubal Ectopic Pregnant Tissue

Objective To investigate the expression and change of the β3 integrin subunit and fibronectin in normal human oviductal tissue during various phases of the menstrual cycle and tubal ectopic pregnant tissueMethods Samples of normal ( n=29 ) and pregnant fallopian tube ( n=22 ) tissues were obtained from women who had normal cycle and history of normal pregnancy. Normal oviductal tissue samples were divided into 4 groups based on their menstrual cycle. Both expression and distribution of the β3 subunit and fibronectin were determined with the immunohistochemical method and the image analysis.Results The β3 subunit was expressed in the cytoplasm of ciliated cells. The expression level of the β3 subunit was higher after ovulation than that before ovulation in isthmus epithelium (P<0.001), and declined significantly after ovulation in ampullae epithelium (P<0.001). In umbrella epithelium within 4 days after ovulation, the expression level of the β3 subunit was observed at rather higher level among other phases (P <0.001). The ciliated and secretory cells of the epithelium except for where the pregnancy occurred in tubal pregnancy expressed the β3 subunit, and no significant relationship was found between the normal tubal tissue of the secretory phase and tubal ectopic pregnant tissue (P>0.05). Fibronectin was expressed in the basement membrane of human oviductal epithelium and matrix. The expression level of fibronectin was higher in the hyperplastic phase than that in the secretory one (P<0.001). And it was lower in normal tubal tissue of the secretory phase than that in tubal ectopic pregnant tissue (P<0.001).Conclusion Theβ3 integrin subunit was expressed in the ciliated cells of human oviductal epithelium, and fibronectin was expressed in the basement membrane of human oviductal epithelium and matrix. Their expression and change in oviductal tissue is based on different phases of menstrual cycle. The β3 subunit could not related to the occurrence of tubal ectopic pregnancy. Fibronectin could be the potential molecular basis for the tubal ectopic pregnancy.

腹腔镜手术治疗输卵管妊娠30例临床观察

目的:探讨腹腔镜手术治疗输卵管妊娠的临床应用价值。方法:纳入输卵管妊娠患者60例,随机分为腹腔镜组和开腹组,各30例。腹腔镜组予腹腔镜手术治疗,开腹组按常规开腹手术治疗。观察两组患者的手术时间、术中出血量、术后肛门排气时间、尿HCG转阴时间、术后平均住院时间及手术并发症情况。结果:与开腹组比较,腹腔镜组术中出血量较少、术后肛门排气时间明显减短、平均住院时间明显缩短,经统计学检验,差异有明显统计学意义(P<0.05);腹腔镜组和开腹组在手术时间、尿HCG转阴时间比较无显著性差异(P>0.05)。结论:腹腔镜手术治疗输卵管妊娠,其创伤小、出血量少、术后恢复快、并发症少、疗效确切,可以保留患者的生育功能,提高其生存质量。