Extracellular Enzymatic Activity of <i>Tuber maculatum</i>and <i>Tuber aestivum</i>Mycelia

Truffle mycelia exhibit a complex interaction pattern with host plants and have been extensively studied over the last years as a source of new bioactive compounds. Fungal enzymes possess a wide use in food industry, confectionaries, textiles and leather industries in order to simplify the processing of raw materials. They are often more stable than enzymes derived from other sources. Tuber maculatum and Tuber aestivum mycelia were tested for enzymes production in Petri dishes solid medium conditions. The results showed that Tuber maculatum produced seven extracellular enzymes (amylase, xylanase, laccase, lipase, peroxidase, cellulase and catalase) while Tuber aestivum produced only three enzymes (amylase, peroxidase and catalase).

印度块菌、夏块菌与化香树合成菌根苗技术初探

印度块菌(Tuber indicum Vittadini)、夏块菌(T.aestivum Vittadin)i是经济价值较高的菌根食用菌。本文对印度块菌、夏块菌与化香树(Platycarya strobilacea Sieb.et Zucc).合成菌根苗的技术进行了研究,结果表明用印度块菌、夏块菌孢子液接种化香树苗后,第三个月发现已形成根尖淡乳色,基部淡黄色、黄褐色的外生菌根。外生菌根为单轴状、羽状和不规则分支状,单根菌根长300μm^4 000μm,直径为200μm^500μm;平坦或从菌根延伸出外延菌丝。印度块菌与化香树形成的外延菌丝为透明、半透明、絮状。夏块菌与化香树形成的外延菌丝为黄绿色、羊毛状。菌根老化后变暗褐或萎缩;两种块菌与化香树形成的菌根在显微镜下有明显的差别。

黑孢块菌、夏块菌菌根苗培育及菌根检测技术研究

本文研究了黑孢块菌和夏块菌的云南松菌根苗培育技术和检测技术,分别介绍了黑孢块菌和夏块菌菌根苗的培育方法及其宏观和显微特征。

西南地区夏块菌子实体内的细菌多样性分析

以夏块菌(Tuber aestivum)为研究对象,采用传统室内分离培养的方式对其子实体内细菌菌株分离培养,并对比筛选,对云南省保山市和四川省会东县的夏块菌子实体内细菌多样性进行研究。结果表明,所分离出的细菌属于5门11属19种,其中,γ-变形菌门所占比例最大,为67%,假单胞菌属的Pseudomonas fuscovaginae为优势类群;厚壁菌门(Firmicutes)所占比例其次,为第二大群,以葡萄球菌属的Staphylococcus simiae、Staphylococcus warneri为优势类群。此外还分离得到拟杆菌门(Bacteroidetes)细菌22株,占9%;α-变形菌门细菌14株,占6%;β-变形菌门6株,占3%。

块菌与板栗培育菌根苗技术探讨

印度块菌(Tuber indicum Vittadini)、夏块菌(Tuber aestivum Vittadini)和白块菌(Tuber panzhihuanense Deng&Wang)是经济价值较高的菌根食用菌。通过对印度块菌、夏块菌和白块菌与板栗(Castanea mollissima Blume)合成菌根苗的技术进行探讨,结果表明使用印度块菌、夏块菌和白块菌孢子液接种板栗树苗,10个月后均能形成大量外生菌根。菌根苗移栽后,接种夏块菌的板栗苗苗高最高,与接种白块菌和印度块菌的板栗苗相比高差异显著,但接种白块菌的板栗苗与接种印度块菌的板栗苗相比苗高差异不显著。接种夏块菌的板栗苗与接种白块菌板栗苗间地径差异不显著,二者与接种印度块菌的板栗苗间地径差异均显著。