11.4.Lung infection

920313 Re-estimation on the role of artifi-cial pneumoperitoneum in treating pulmon-ary tuberculosis heamoptysis.SHEN Zhuangfu(沈庄夫),et al.Zhejiang Ningbo No.1 Hosp,315000.Chin J Tuberc & Respir Dis 1991;14(6):

11.4.Lung infection

930301 Evaluation of detection of M.tubercu-losis in clinical specimens of tuberculosis byDNA amplification.ZHUANG Yuhui (庄玉辉),et al.309th Hosp,PLA.Chin J Tuberc & RespirDis 1993;16(1):26—29.The sensitivity of detection of M tuberculosisgenomic DNA were lpg～100fg or 10～100 bac-terial cell—by PCR.Only M,tuberculosis,M,bovis and BCG were positive with 165 b.pband,but all other 14 mycobacterium and 10bacteria of nonmycobacterial tested were nega-tive.Of 75 sputum specimens of pulmonary tu-berculosis,the positive rate of PCR was 53.3%,while culture method was 21.3% and

Prevention

950429 Implementation of national tuberculosis con-trol programme(focus on registration,case-manage-ment and treatment).QIAN Yuanfu(钱元福),et al.Nation Tuberc Contr Center.Public Health Ministr,Beijing,101149.Chin J Tuberc & Respir Dis 1995;18(1):16-18.The results of three nationwide epidemiological sur-veys on tuberculosis showed that the declination in theincidence of tuberculosis from 1979 to 1990 was slowThe rates of case registration and new case registrationrates within ten years(1982～1991) were graduallyincreasing year by year and came to a climax in 1988.

Lung infection

950304 The diagnosis and treatment of pulmonaryaspergilloma in the aged—a report of 17 cases.LI Di-anqin(李殿清),et al.Henan Provincial Pulmon DisHosp,Zhengzhou,450003.Chin J Geriatr 1994;13(6):338-339.Seventeen cases of pulmonary aspergilloma in theaged were reported.The primary diseases were pul-monary tuberculosis in 14 cases and pulmonary cyst,cancer of lung and pulmonary abscess in one each.In14 cases,the clinical manifestation was frequenthemoptysis;the occurrence rate was 82.4%.

Others

930453 The diagnosis of tuberculous meningitisby using polymerase chain reaction.PAN Yuxu-an (潘毓宣),et al.Beijing Tuberc & ThoracicTumor Res Instit,Beijing,101149.Chin J Tuberc&.Respir Dis 1993;16(2):77—78.

Lung infection

930120 A clinical study of 50 cases of legion-naires disease.WANG Baofa(王保法),et al.Dept Intern Med,2nd Affili Hosp,Hehei MedColl,Shijiazhuang,050000.Chin J Tuberc &Respir Dis 1992;15(5):266-268.The clinical features and X-ray manifesta-tions of 50 cases of legionnaires disease wereanalysed.8 cases might be due to nosocomial in-fection through breathing in flying particles ofthe saliva or phlegm.According to the mainclinical features,this disease could be dividedinto common pneumonia type,acute gastroen-teritis type,encephalopathy type,shock type,and acute renal insufficiency type.The differen-

大肠杆菌-分枝杆菌分泌型穿梭表达质粒pBCG-SP-HSP65的构建及人结核杆菌HSP65的表达

用PCR技术从BacillusCalmette gu啨ｒｉｎ(BCG)基因组中扩增出抗原 85B(Ag85B)的信号肽 (SP)DNA序列 ,从pCMVMTHSP65质粒中扩增出人结核杆菌HSP65全长基因。利用DNA重组技术将以上两个片段插入质粒pBCG 2 10 0的人结核杆菌HSP70启动子下游 ,构建成分泌型原核穿梭表达质粒 (pBCG SP HSP65)。酶切鉴定、PCR和测序分析结果均表明分泌型原核穿梭表达质粒pBCG SP HSP65构建成功。利用电穿孔将该质粒转入耻垢分枝杆菌(Mycobacterialsmegmatis,MS)中 ,用卡那霉素筛选出阳性重组子。经热诱导后用SDS PAGE观察到在耻垢分枝杆菌中 65kD蛋白占总蛋白的 2 0 % ,而在重组耻垢分枝杆菌表达的 65kD蛋白占菌体总蛋白的 3 4 46% ,占裂解物上清总蛋白的 68 56% ,表明重组的HSP65基因能在耻垢分枝杆菌中高效表达 ,表达的蛋白大部分以可溶状态存在。通过Western blot证实分泌的该蛋白能与结核杆菌HSP65的抗体特异性结合 ,说明该重组蛋白具有HSP65的生物学活性。

噬菌体扩增技术快速检测结核分枝杆菌的临床应用

结核病细菌学检查主要靠痰涂片和培养的方法，阳性率只有30％左右。结核菌培养则需1-2mo。结核抗体检测假阳性率、假阴性率都很高。FAST-plaqueTB噬菌体扩增技术检测结核杆菌，具有特异性和灵敏度高的优点，而且快速、简便。

10.4.Cor pulmonale

920074 Influence of atmospheric pressurefluctuation on condition of patients with corpulmonale.LI Jmgzhou (李景周),et al.1st Hosp,Fangshan District,Beijing,102400.Chin J Tuberc& Respir Dis 1991; 14 (4): 231-232.Based on investigation of 632 patients with corpulmonale,we found that there were a closecorresponding relationships of attack,exacerba-

Lung function

950300 The enhancing effects of the supernatant ofpulmonary alveolar macrophages stimulated by BCGon proliferation of pulmonary fibroblasts.ZHANGShangming(张尚明),et al.North-China Calo MedCoil,Tangshan,063000.Chin J Tuberc & Respir Dis1995;18(1):26-28.The supernatant of pulmonary alveolar macrophages(PALM) stimulated by 7.5～60mg/L of BCG could sig-nificantly enhance proliferation of pulmonary fibrob-lasts (PFB),and the supernatant contained high levelof TNF.This result indicated BCG could

Lung function

920669 Examination of maximum trans-diaphragmatic pressure and diaphragm elec-tromyography in patients with obstructiveemphysema. YU Bingxiang(余秉翔), et al.Dept Pul Med, General Hosp, PLA, Beijing,100853 Chin J Tuberc ＆ Respir Dis 1992;15(3): 155-157.

Respiratory tract tumor

920675 Estrogea receptor in female lungcarcinoma. YANG Meihuai(杨梅怀), et al.Dept Pathol. Qingdao Med Coil, 266012. ChinJ Tuberc & Respir Dis 1992; 138-140. Eighty cases of female lung carcinoma wereassayed with avidinbiotin complex method forestrogen receptor(ER)and 73. 8％ cases

6.3.Immunity

920045 The effect of silicon on the tubercu-lous infection and immune system.SHENFuzhen (沈福珍).Shanghai 1st Pulmon Hosp,200433.Chin J Tuberc & Respir Dis 1991; 14 (5):292-294Our study showed that silicon not only injuredChina Medical Abstracts (Internal Medicine)

11.1.Lung function

920305 The effect of nifedipine on pulmon-ary hemodynamics after injection of oleicacid in rabbits.TANG Jifei (唐疾飞),et al.CorPulmon Res Lab,Wenzhou Med Coll,325003.ChinJ Tuberc & Respir Dis 1991;14(6):347-349.

Lung function

930113 A comparative study of the effects ofcontrol ventilation and synchronized intermit-tent mandatory ventilation on hemodynamicsand blood gases in patients with chronic obstruc-tive pulmonary disease.BAI Chunxue（白春学）,et al.Zhongshan Hosp,Shanghai Med Univ,200032.Chin J Tuberc & Respir Dis 1992;15（4）:220-221.Effects of control ventilation（CV）and syn-chronized intermittent mandatory ventilation（SIMV）were comparatively studied in 9 pa-tients with chronic obstructive pulmonary dis-ease.Cardiac index(from 2.99±0.65 to 3.60±0.651·min-1·m-2,P【0.05)and oxygen de-

Cor pulmonale

930475 The study of prethrombotic state in pa-tients with COPD and cor pulmonale.MA Duan（马端）,et al.Thrombosis & HaemostasisLab,Shandong Traffic Hosp,Jinan,250031.ChinJ Tuberc & Respir Dis 1993;16（2）:95—97.We measured PT,TT,KPTT,Fg,vWF,AT—IILAg,AT—ⅢA,a2M,TXB2,6—keto—PGFla,PLg,tPAAg,tPAA and PAl of patients with a-cute onset of chronic bronchitis,pulmonary em-physema,and cor pulmonale.The results

Interstitial lung disease

930124 The effect of glycosaminoglycans inthe genesis of pulmonary interstitial fibrosis.LIBaoyu(李保玉),et al.Dept Pathol,Jilin MedColl,132001.Chin J Tuberc & Respir Dis 1992;15(4):204-205.The pulmonary interstitial fibrosis was causedby injecting Bleomycin into mouse trachea.Afterthe injection,the volume of glycosaminoglycans(GAG)in bronchoalveolar lavage fluid and lungtissues was increased.The observation underhistochemical stain and electron microscopeshowed that the distribution of GAG in lung tis-sues was varied at different time after the injec-tion,and related to the volume of collagen pro-teins and the formation of pulmonary interstitialfibrosis.

Interstitial lung disease

920673 The role of oxygen radicals in BLM-induced pulmonary fibrosis. WANGXiaozhi(王晓芝), et al. Respir Dis Res Lab,Union Hosp, Tongji Med Univ, Wuhan, 430022.Chin J Tuberc & Respir Dis 1992; 15(3): 158-160.

Others

950250 Rapid detection of mycoplasma pneumoniaein clinical samples by the polymerase chain reactiontechnique.LI Ziling(李子玲),et al.General Hosp,Nanjing Command,Nanjing,210002.Chin J Tuberc &Resspir Dis 1995;18(1):41—43.The polymerase chain reaction(PCR) technique wasused to detect Mycoplasma pneumoniae in clinical sam-ples(bronchoalveolar lavage fluids and throat swabs).A specific DNA sequence for M.pneumoniae was se-lected form a genomic library.The amplification targetregion was partial DNA sequence of the 500-bp frag-

11.7.Respiratory tract tumor

920105 Observation on the activity of su-peroxide dismutase and catalase of alveolarmacrophage in patients with Iung cancer.TANG Zuping (唐祖平),et al.1st Hosp。