Liver cancer,and in particular hepatocellular carcinoma(HCC)is a disease of rising prevalence and incidence.To date,definitive treatment options include either surgical excision or ablation of the affected area.With i...Liver cancer,and in particular hepatocellular carcinoma(HCC)is a disease of rising prevalence and incidence.To date,definitive treatment options include either surgical excision or ablation of the affected area.With increasing research on several pathways that could be involved in the progression of HCC,new elements within these pathways emerge as potential targets for novel therapies.The WNT/β-catenin pathway favors the presence of M2 tumor-associated macrophages which in turn promote tumor growth and metastasis.The inhibition of this pathway is considered a good candidate for such targeted therapeutic interventions.Interestingly,as Huang et al show in their recently published article,Calculus bovis which is used in traditional Chinese medicine can exert an inhibitory effect on theβ-catenin pathway and become a potential candidate for targeted pharmacotherapy against liver cancer.展开更多
BACKGROUNDAdvanced gastric tumors are extremely prone to metastasize the in 20%–30% ofgastric cancer, and patients have a poor prognosis despite systemic chemotherapy.Peritoneal metastases from gastric cancer usually...BACKGROUNDAdvanced gastric tumors are extremely prone to metastasize the in 20%–30% ofgastric cancer, and patients have a poor prognosis despite systemic chemotherapy.Peritoneal metastases from gastric cancer usually indicate the end stageof the disease without curative treatment.AIMTo peritoneal metastasis for facilitating clinical therapy are urgently needed.METHODSImmunohistochemical staining and immunofluorescence staining were used todemonstrate the high expression of cathepsin L (CTSL) in human gastric cancertissues and its localization in cells. Lentivirus transfection was used to construct stable cell lines. Transwell invasion assays, wound healing assays, and animal tests were used to determine therelationships between CTSL and epithelial-mesenchymal transition (EMT) and tumorigenic potential in vivo.RESULTSWe observed that macrophage-derived CTSL promoted gastric cancer cell migration and metastasis via the EMTpathway in vitro and in vivo, which involved macrophage polarization. Our findings suggest that macrophagesimprove extracellular matrix remodeling and hence facilitate tumor metastasis. Ablation of CTSL in macrophageswithin the tumor microenvironment may improve tumor therapy and the prognosis of patients with gastric cancerperitoneal metastasis.CONCLUSIONIn consideration of our findings, tumor-associated macrophage-derived CTSL is an important factor that promotesthe metastasis and invasion of gastric cancer cells, and the targeting of CTSL may potentially improve the prognosisof patients with gastric cancer with peritoneal metastasis.展开更多
文摘Liver cancer,and in particular hepatocellular carcinoma(HCC)is a disease of rising prevalence and incidence.To date,definitive treatment options include either surgical excision or ablation of the affected area.With increasing research on several pathways that could be involved in the progression of HCC,new elements within these pathways emerge as potential targets for novel therapies.The WNT/β-catenin pathway favors the presence of M2 tumor-associated macrophages which in turn promote tumor growth and metastasis.The inhibition of this pathway is considered a good candidate for such targeted therapeutic interventions.Interestingly,as Huang et al show in their recently published article,Calculus bovis which is used in traditional Chinese medicine can exert an inhibitory effect on theβ-catenin pathway and become a potential candidate for targeted pharmacotherapy against liver cancer.
基金Supported by The National Natural Science Foundation of China,No.82272087 and No.82103150The Guangdong Natural Science Foundation Outstanding Youth Project,China,No.2021B1515020055The Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Cancer,China,No.2020B121201004.
文摘BACKGROUNDAdvanced gastric tumors are extremely prone to metastasize the in 20%–30% ofgastric cancer, and patients have a poor prognosis despite systemic chemotherapy.Peritoneal metastases from gastric cancer usually indicate the end stageof the disease without curative treatment.AIMTo peritoneal metastasis for facilitating clinical therapy are urgently needed.METHODSImmunohistochemical staining and immunofluorescence staining were used todemonstrate the high expression of cathepsin L (CTSL) in human gastric cancertissues and its localization in cells. Lentivirus transfection was used to construct stable cell lines. Transwell invasion assays, wound healing assays, and animal tests were used to determine therelationships between CTSL and epithelial-mesenchymal transition (EMT) and tumorigenic potential in vivo.RESULTSWe observed that macrophage-derived CTSL promoted gastric cancer cell migration and metastasis via the EMTpathway in vitro and in vivo, which involved macrophage polarization. Our findings suggest that macrophagesimprove extracellular matrix remodeling and hence facilitate tumor metastasis. Ablation of CTSL in macrophageswithin the tumor microenvironment may improve tumor therapy and the prognosis of patients with gastric cancerperitoneal metastasis.CONCLUSIONIn consideration of our findings, tumor-associated macrophage-derived CTSL is an important factor that promotesthe metastasis and invasion of gastric cancer cells, and the targeting of CTSL may potentially improve the prognosisof patients with gastric cancer with peritoneal metastasis.
