Two novel polyhydroxylated steroidal sapogenins, wattigenin B ((25R)-spirost-1beta, 2beta, 3beta, 4beta, 5beta, 6beta, 7alpha-heptol, 1) and wattigenin C ((25S)-spirost-1beta, 2beta, 3beta, 4beta, 5beta, 7alpha-hexalr...Two novel polyhydroxylated steroidal sapogenins, wattigenin B ((25R)-spirost-1beta, 2beta, 3beta, 4beta, 5beta, 6beta, 7alpha-heptol, 1) and wattigenin C ((25S)-spirost-1beta, 2beta, 3beta, 4beta, 5beta, 7alpha-hexalrydroxyl-6-one, 2), together with two known sapogenins, kitigenin (3) and convallagenin B (4), were isolated froth the fresh rhizomes of Tupistra wattii Hook. f. The structures of the compounds were determined on the basis of spectroscopic analysis. The four sapogenins were evaluated for the cytotoxicities on the cancer cell line K562 and A2780a in vitro. Compounds 1 - 4 were obtained from the plant for the first time.展开更多
A pair of diastereoisomeric furostanol saponins was obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Their st...A pair of diastereoisomeric furostanol saponins was obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Their structures were determined, on the basis of chemical and spectroscopic evidences.展开更多
Two furostanol saponins were obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Their structures were determine...Two furostanol saponins were obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Their structures were determined as （25S）-26-O-（β-D-glucopyranosyl）- furost-1β, 3β, 22α, 26-tetrol-3-O-β-D-glucopyranosyl-（1 → 4）-β-D-glucopyranosyl-（1 → 2）-β-D-glucopyranoside （1） and （25R）- 26-O-（β-D-glucopyranosyl）-furost-1β, 3β 22a, 26-tetrol 3-O-β-D-glucopyranosyl-（1 → 4）-β-D-glucopyranosyl-（1 → 2）-β-D-glu- copyranoside （2）, on basis of chemical and spectroscopic evidences. 1 and 2 displayed marked inhibitory action towards COX-2 production in macrophages of the rat abdomen induced by LPS at 20 μg/mL.展开更多
Two new spirostanol saponins, (25S)-spirostane-1β,3β,5β-triol 3-O-β-D-glucopyrano- side 1 and rhodeasapogenin 3-O-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranoside 2, together with a known saponin, rhodeasapogenin...Two new spirostanol saponins, (25S)-spirostane-1β,3β,5β-triol 3-O-β-D-glucopyrano- side 1 and rhodeasapogenin 3-O-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranoside 2, together with a known saponin, rhodeasapogenin 3-O-β-D-glucopyranoside 3, were isolated from the underground parts of Tupistra chinensis Bak.. Their structures were determined by spectroscopic analysis.展开更多
A furostanol saponin was obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Its structure was determined as 3-O...A furostanol saponin was obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Its structure was determined as 3-O-β-D-glucopyranosyl-（25S）-22-O-methyl-5β-furost-1β, 3β, 5β, 22α; 26-pentaol-26-O-β-D-glucopyranoside （1） on the basis of chemical and spectroscopic evidences. The n-butanol fraction displayed marked inhibitory activity in vitro towards HeLa and HL-60 human tumor cell lines by MTT method.展开更多
This study examined the effect of saponins from Tupistra chinensis Bak （STCB） on the growth of sarcoma S-180 cells in vitro and in mouse xenografts as well as the underlying mechanisms. Cell proliferation was assess...This study examined the effect of saponins from Tupistra chinensis Bak （STCB） on the growth of sarcoma S-180 cells in vitro and in mouse xenografts as well as the underlying mechanisms. Cell proliferation was assessed by MTT assay. Cell cycle distribution was determined by flow cytometry. Sarcoma S-180 tumor-bearing mice were treated with different doses of STCB with 10 μg/mL 5-fluorouracil （5-Fu） as a positive control. The activity of nuclear factor （NF）-κB was detected by gel mobility shift assay. The mRNA level of NF-κB was determined by real-time quantitative RT-PCR. The results showed that in vitro STCB inhibited the growth of S-180 cells in a concentration-dependent manner, which was accompanied by cell cycle arrest at S-phase. In vivo STCB significantly inhibited the growth of S-180 tumor mouse xenografts in a dose-dependent manner with apparent induction of cell apoptosis. Moreover, STCB inhibited the activity of NF-rd3 p65 and reduced the expression of NF-κB p65 mRNA in mouse xenografts. It was concluded that STCB inhibits the proliferation and cell cycle progression of S- 180 cells by suppressing NF-κB signaling in mouse xenografts. Our findings suggest STCB is a promising agent for the treatment of sarcoma.展开更多
Five new polyhydroxylated furostanol saponins were isolated from the roots and rhizomes of Tupistra chinensis,and their structures were determined as tupistrosides J–N(1–5),together with four known furostanol saponi...Five new polyhydroxylated furostanol saponins were isolated from the roots and rhizomes of Tupistra chinensis,and their structures were determined as tupistrosides J–N(1–5),together with four known furostanol saponins(6–9),on the basis of physico-chemical properties and spectral analysis.Among them,compounds 3 and 5 showed cytotoxicity against human cancer cell lines SW620 with IC50 values of 72.5±2.4 and 77.3±2.5μmol·L^–1,respectively.Compound 4 showed cytotoxicity against human cancer cell line HepG2 with IC50 value of 88.6±2.1μmol·L^–1.展开更多
目的探讨开口箭、筒鞘蛇菰提取物的醒酒作用。方法采用行为学指标,探讨酒精对小鼠的致醉量及开口箭、筒鞘蛇菰提取物对急性酒精中毒小鼠的翻正反射和自发活动的影响。结果39度白酒对小鼠的致醉量为0.34 m l.(20 g)-1;小鼠给酒致醉后,开...目的探讨开口箭、筒鞘蛇菰提取物的醒酒作用。方法采用行为学指标,探讨酒精对小鼠的致醉量及开口箭、筒鞘蛇菰提取物对急性酒精中毒小鼠的翻正反射和自发活动的影响。结果39度白酒对小鼠的致醉量为0.34 m l.(20 g)-1;小鼠给酒致醉后,开口箭、筒鞘蛇菰提取物对其翻正反射维持时间的影响明显,与模型组相比有显著性差异(P<0.05)。自发活动实验显示,开口箭、筒鞘蛇菰醇提取物的醒酒效果均比水提取物的效果要好。结论开口箭、筒鞘蛇菰提取物具有醒酒作用,值得进一步研究。展开更多
文摘Two novel polyhydroxylated steroidal sapogenins, wattigenin B ((25R)-spirost-1beta, 2beta, 3beta, 4beta, 5beta, 6beta, 7alpha-heptol, 1) and wattigenin C ((25S)-spirost-1beta, 2beta, 3beta, 4beta, 5beta, 7alpha-hexalrydroxyl-6-one, 2), together with two known sapogenins, kitigenin (3) and convallagenin B (4), were isolated froth the fresh rhizomes of Tupistra wattii Hook. f. The structures of the compounds were determined on the basis of spectroscopic analysis. The four sapogenins were evaluated for the cytotoxicities on the cancer cell line K562 and A2780a in vitro. Compounds 1 - 4 were obtained from the plant for the first time.
文摘A pair of diastereoisomeric furostanol saponins was obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Their structures were determined, on the basis of chemical and spectroscopic evidences.
基金financially supported by the National Natural Science Foundation of China(No.30670213)Key Scientific Program of China Three Gorges University(No.2005ZD007).
文摘Two furostanol saponins were obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Their structures were determined as （25S）-26-O-（β-D-glucopyranosyl）- furost-1β, 3β, 22α, 26-tetrol-3-O-β-D-glucopyranosyl-（1 → 4）-β-D-glucopyranosyl-（1 → 2）-β-D-glucopyranoside （1） and （25R）- 26-O-（β-D-glucopyranosyl）-furost-1β, 3β 22a, 26-tetrol 3-O-β-D-glucopyranosyl-（1 → 4）-β-D-glucopyranosyl-（1 → 2）-β-D-glu- copyranoside （2）, on basis of chemical and spectroscopic evidences. 1 and 2 displayed marked inhibitory action towards COX-2 production in macrophages of the rat abdomen induced by LPS at 20 μg/mL.
文摘Two new spirostanol saponins, (25S)-spirostane-1β,3β,5β-triol 3-O-β-D-glucopyrano- side 1 and rhodeasapogenin 3-O-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranoside 2, together with a known saponin, rhodeasapogenin 3-O-β-D-glucopyranoside 3, were isolated from the underground parts of Tupistra chinensis Bak.. Their structures were determined by spectroscopic analysis.
文摘A furostanol saponin was obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Its structure was determined as 3-O-β-D-glucopyranosyl-（25S）-22-O-methyl-5β-furost-1β, 3β, 5β, 22α; 26-pentaol-26-O-β-D-glucopyranoside （1） on the basis of chemical and spectroscopic evidences. The n-butanol fraction displayed marked inhibitory activity in vitro towards HeLa and HL-60 human tumor cell lines by MTT method.
文摘This study examined the effect of saponins from Tupistra chinensis Bak （STCB） on the growth of sarcoma S-180 cells in vitro and in mouse xenografts as well as the underlying mechanisms. Cell proliferation was assessed by MTT assay. Cell cycle distribution was determined by flow cytometry. Sarcoma S-180 tumor-bearing mice were treated with different doses of STCB with 10 μg/mL 5-fluorouracil （5-Fu） as a positive control. The activity of nuclear factor （NF）-κB was detected by gel mobility shift assay. The mRNA level of NF-κB was determined by real-time quantitative RT-PCR. The results showed that in vitro STCB inhibited the growth of S-180 cells in a concentration-dependent manner, which was accompanied by cell cycle arrest at S-phase. In vivo STCB significantly inhibited the growth of S-180 tumor mouse xenografts in a dose-dependent manner with apparent induction of cell apoptosis. Moreover, STCB inhibited the activity of NF-rd3 p65 and reduced the expression of NF-κB p65 mRNA in mouse xenografts. It was concluded that STCB inhibits the proliferation and cell cycle progression of S- 180 cells by suppressing NF-κB signaling in mouse xenografts. Our findings suggest STCB is a promising agent for the treatment of sarcoma.
基金supported by the Open Research Fund of Key Laboratory of Basic and New Herbal Medicament Research,Shaanxi university of Chinese medicine(Nos.17JS030 and 2017KF02)Subject Innovation Team of Shaanxi University of Chinese Medicine(No.2019-YL12)+2 种基金the Special Project of Shaanxi Province Education Department(No.14JF005)the National Natural Sciences Foundation of China(No.81503237)the Key R&D Program of Shaanxi Province(Nos.2018SF-324,2017SF-360 and 2015SF073)
文摘Five new polyhydroxylated furostanol saponins were isolated from the roots and rhizomes of Tupistra chinensis,and their structures were determined as tupistrosides J–N(1–5),together with four known furostanol saponins(6–9),on the basis of physico-chemical properties and spectral analysis.Among them,compounds 3 and 5 showed cytotoxicity against human cancer cell lines SW620 with IC50 values of 72.5±2.4 and 77.3±2.5μmol·L^–1,respectively.Compound 4 showed cytotoxicity against human cancer cell line HepG2 with IC50 value of 88.6±2.1μmol·L^–1.
文摘目的探讨开口箭、筒鞘蛇菰提取物的醒酒作用。方法采用行为学指标,探讨酒精对小鼠的致醉量及开口箭、筒鞘蛇菰提取物对急性酒精中毒小鼠的翻正反射和自发活动的影响。结果39度白酒对小鼠的致醉量为0.34 m l.(20 g)-1;小鼠给酒致醉后,开口箭、筒鞘蛇菰提取物对其翻正反射维持时间的影响明显,与模型组相比有显著性差异(P<0.05)。自发活动实验显示,开口箭、筒鞘蛇菰醇提取物的醒酒效果均比水提取物的效果要好。结论开口箭、筒鞘蛇菰提取物具有醒酒作用,值得进一步研究。
