Objective:To evaluate the antioxidant activities and total phenolic contents of brown seaweeds belonging to Turbinaria spp.[Turbinaria conoides(T.conoides)and Turbinaria ornata(T.ornata)collected from Gulf of Mannar o...Objective:To evaluate the antioxidant activities and total phenolic contents of brown seaweeds belonging to Turbinaria spp.[Turbinaria conoides(T.conoides)and Turbinaria ornata(T.ornata)collected from Gulf of Mannar of southeastern coast of India in various in vitro systems.Methods:The antioxidant activity was evaluated using different in vitro systems,viz.,I,I-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis-3 ethylbenzothiozoline-6-sulfonic acid diammonium salt(ABTS),H_20_2/H0 radical scavenging,Fe^(2+)ion chelating ability,and reducing potential.Folin-Ciocalteu method was used to determine the total phenolic content of the extracts,and the results were expressed as mg of gallic acid equivalents(GE)/g of the seaweed extracts.Thiobarbituric acid-reactive substances assay was employed to assess the ability of the seaweed extracts to inhibit lipid oxidation.Results:Ethyl acetate(EtOAc)fraction of T.conoides registered significantly higher phenolic content(105.97 mg GE/g)than that of T.ornata(69.63 mg GE/g).Significantly higher antioxidant potential as determined by DPPH(64.14%)radical scavenging activity was registered in EtOAc fraction of T.ornata.A higher ABTS(?)radical scavenging(IC_(50)3.16μg/mL),Fe^(2+)chelating(IC_(50)0.46 mg/mL),H_2O_2 scavenging(IC_(50)4.25 mg/mL),lipid peroxidation inhibitory(TBARS,IC_(50)0.21 mg/mL),and reducing abilities(IC_(50)52.67 mg/mL)(P<0.05)were realized in EtOAc fraction of T.ornata than other fractions.Conclusions:This study indicated the potential use of T.conoides and T.ornata as candidate species to be used as food supplements/functional foods to increase shelf-life of food items for human consumption,and nutraceuticals to deter deleterious free radical-induced life-threatening diseases.展开更多
In this study,the biosorption of Malachite green(MG)onto Turbinaria conoides,brown marine algae,was studied with respect to initial pH,temperature,initial dye concentration,and sorbent dosage.The optimum initial pH an...In this study,the biosorption of Malachite green(MG)onto Turbinaria conoides,brown marine algae,was studied with respect to initial pH,temperature,initial dye concentration,and sorbent dosage.The optimum initial pH and temperature values for MG removal were found to be 8.0 and 30℃,respectively.Sorbent dosage was found to strongly influence the removal of MG.Equilibrium studies were carried out to test the validity of the Langmuir(q_(max)=66.6 mg/g and b=0.526 mL mol/L)and the Freundlich(n=1.826 and K=3.751 mg/g)isotherms.The kinetic studies indicated the validity of the pseudo first-order and second-order equation.展开更多
Objective:To evaluate the in vivo acute toxicity and antioxidant activity of the marine endophytic fungus extract S2 isolated from Turbinaria conoides.Methods:Two doses(100 mg/kg and 400 mg/kg)of the S2 extract were a...Objective:To evaluate the in vivo acute toxicity and antioxidant activity of the marine endophytic fungus extract S2 isolated from Turbinaria conoides.Methods:Two doses(100 mg/kg and 400 mg/kg)of the S2 extract were administered to rats orally for acute toxicity and antioxidant test.The body weight,relative weight of six organs,haematological,biochemical and antioxidant properties were investigated on Day 14.Results:A single oral dose treatment did not cause any mortality or observable adverse effects in rats.No significant variations in the body and organ weights between the control and the treated groups were observed.Heamatological analysis and clinical blood chemistry also did not reveal any toxic effects of the extract.The total white blood cell count and haemoglobin levels were increased.The levels of total serum cholesterol in males treated with 100 and 400 mg/kg were significantly(P<0.05)decreased(1.28 and 1.34 mmol/L respectively)compared to control(1.55 mmol/L)rats.Pathologically,neither gross abnormalities nor histopathological changes were observed.This study showed strong evidence of the non-toxic effects of S2 extract.Furthermore the extract exhibited significant(P<0.05)antioxidant activity through increased levels of superoxide dismutase and glutathione peroxidase enzy mes in serum,liver and kidney.Conclusions:The research findings from the present study showed the potential of marine natural products particularly in Malaysia as a source of bioactive compounds.Marine endophytic fungi as a potential source of anticancer drugs with great potential as they are potent yet safe,thus deserving further extensive investigation.展开更多
Objective:To evaluate the antimicrobial capacity of fucoidan from the brown seaweed Turbinaria ornata against the marine ornamental fish bacterial pathogens.Methods:Fucoidan was isolated by the ethanol extraction meth...Objective:To evaluate the antimicrobial capacity of fucoidan from the brown seaweed Turbinaria ornata against the marine ornamental fish bacterial pathogens.Methods:Fucoidan was isolated by the ethanol extraction method and the functional groups were identified by Fourier Transform Infrared Spectroscopy analysis.Subsequently,structural characterization was done by 1H NMR analysis.In vitro antibacterial activity of fucoidan was performed by the agar plate diffusion method and Minimum Inhibitory Concentration.Results:The characteristic C-0-S bending vibration of sulfate substituent the axial C-4 was observed at 839 cm^(-1).Characterisitic signal of the fucoidan was detected in a different ppm of 1H NMR analysis.The maximum antibacterial activity(16.23±0.11)mm was obtained for Vibrio parahaemolyticus and the minimum activity(5.1±0.24)mm was recorded for Yersinia enterocolitica.The Minimum Inhibitory Concentration value was recorded between 2.5 to 10 mg/mL to the respective pathogens.Conclusions:The present study proved that fucoidan possessed the significant antibacterial activity against the tested fish bacterial pathogens.It could be further used as a natural antibiotic in an aquaculture system to control the bacterial diseases.However,the present study suggested that the further in vivo study is required to get the better understanding of the mode of action of fucoidan.展开更多
Objective:To study ecological parameters and biochemical composition of brown seaweed,Turbinaria ornata(T.ornata)collected from Hurghada shores,Red Sea coast of Egypt during September,October and November,2015.Methods...Objective:To study ecological parameters and biochemical composition of brown seaweed,Turbinaria ornata(T.ornata)collected from Hurghada shores,Red Sea coast of Egypt during September,October and November,2015.Methods:T.ornata and its associated seaweeds were collected,identified and their abundances were estimated.Water of collection site was analyzed physicochemically as well as qualitative and quantitative analyses of phytoplankton.T.ornata was analyzed for protein,total carbohydrate,lipids,alginic acid,agar,pigments,minerals and heavy metals.Results:The results showed that macroalgal species recorded along Hurghada shores belong to Phaeophyta,Rhodophyta and Chlorophyta.At collection site,the moderate temperature,slight alkaline pH,low turbidity,high dissolved oxygen and valuable nutrient content of saline water exerted the massive growth of T.ornata with maximum abundance(24%)during October.The phytoplankton community was quite diverse with a maximum numbers of taxa(104.2×10^(8) cell/L)recorded during October.Analysis of T.ornata alga powder showed that high soluble carbohydrate(2.80±0.10 mg/g dry/weight)and chlorophyll c(0.0017±0.0001 mg/g fresh weight)contents were recorded during September;while high contents of protein(37.70±0.60 mg/g dry weight),lipids(3.10±0.06 mg/g dry weight),polysaccharides(agar and alginates),carotenoids(0.0160±0.0004 mg/g fresh weight),minerals and heavy metals were recorded during November.Conclusions:The study revealed that physicochemical analyses of water were varied slightly during the three months and suitable for the growth of T.ornata.It contains high amount of most biochemical constituents during October.展开更多
Objective:To investigate the potential antibacterial activities of ethanol extracts of Turbinaria ornata(T.ornata),Oleic acid(OA)and palmitic acid(PA)extracted from T.ornata as well as mixtures of OA and PA(1:1)agains...Objective:To investigate the potential antibacterial activities of ethanol extracts of Turbinaria ornata(T.ornata),Oleic acid(OA)and palmitic acid(PA)extracted from T.ornata as well as mixtures of OA and PA(1:1)against some bacterial species.Methods:Brown seaweed T.ornata was collected from Hurghada shores,Red Sea coast of Egypt.OA and PA were extracted from T.ornata.Ethanol extracts of T.ornata,OA,PA and mixtures of these two fatty acids(1:1)were tested for their antibacterial activities against Escherichia coli,Bacillus cereus,Bacillus subtilis,Klebsiella pneumonia and Staphylococcus aureus by the disc diffusion method.Results:Ethanol extracts of T.ornata,OA,PA and mixtures of OA and PA(1:1)showed antibacterial activities that increased significantly(least significant difference at 0.05 level)with increasing concentrations against all tested bacteria.Different concentrations of ethanol extracts of T.ornata and extracted OA showed its highest activity against Bacillus subtilis,while PA and mixtures of PA and OA(1:1)showed its highest activity against Bacillus cereus.The maximum inhibition activities were shown for mixtures of OA and PA(1:1).Scanning electron microscope showed that mixtures of OA and PA(1:1)caused plasmolysis and reduction in cell size of Escherichia coli.Conclusions:Different concentrations of T.ornata and its fatty acids showed activities against all tested bacteria.Therefore,it is a potential source of natural antimicrobial compounds.展开更多
Objective:To evaluate the antimicrobial activity of Turbinaria conoides(T.conoides).Padina gymnospora(P.gymnospora) and Sargassum tenerrimum against human bacterial and fungal pathogens.Methods:The antimicrobial activ...Objective:To evaluate the antimicrobial activity of Turbinaria conoides(T.conoides).Padina gymnospora(P.gymnospora) and Sargassum tenerrimum against human bacterial and fungal pathogens.Methods:The antimicrobial activities of the extracts against various organisms were tested by using disc diffusion method.Results:The methanol extract showed the better result than the other extracts.Whereas,the strong antibacterial inhibition was noted in methanol extracts of P.gymnospora against Bacillus subtilus(26.33±1.86) and the mild inhibition of ethanol extracts from T.conoides against Klebsiella pneumoniae(2.33±0.51).Acetone extraction of P. gymnospora had strong antifungal inhibition against Cryptococcus neoformans(23.00±1.78), and acetone extract of T.conoides had mild inhibition against Aspergillus niger(3.00±0.89). Conclusions:The seven different solvent extracts of seaweeds used in the present study have shown significant bacterial action.Further,a detailed study on the principle compound in the seaweeds which is responsible for antimicrobial activity is still needed and it can be achieved by using advanced separation techniques.展开更多
Introduction: Intestinal bacteria are exposed many external influences, including drugs, causing the emergence of strains resistant to the effects of antibiotics. Consequently, the discovery of new antibiotics that af...Introduction: Intestinal bacteria are exposed many external influences, including drugs, causing the emergence of strains resistant to the effects of antibiotics. Consequently, the discovery of new antibiotics that affect resistant strains is required. Marine algae offer a source of renewable natural compounds with antimicrobial effects. Therefore, the aim of this study was to detect some of these compounds and examine their impact on enteric bacteria. Methodology: Escherichia coli, Salmonella typhi, Shigella dysenteriae, Klebsiella pneumoniae, and Enterobacter aerogenes were tested with extracts of Turbinaria triquetra and Halimeda opuntia extracted with methanol, ethanol, petroleum ether, or dimethyl formamide solvents. We measured bacterial growth inhibition, the minimal inhibitory concentrations (MICs), and potassium leakage, and analyzed the bacterial cells with scanning electron microscopy and energy-dispersive X-ray spectroscopy. Results: The T. triquetra extract produced with methanol strongly affected the bacteria tested. When the results for T. triquetra and H. opuntia were compared with those of omacillin, the T. triquetra and H. opuntia extracts in most solvents were more effective than the antibiotic. Differences in the bacterial growth inhibition and MICs depended on the type of alga and the solvent used. At the end of the incubation period, potassium leakage had increased by 62.98% for E. coli, 61.24% for S. typhi, 61.32% for S. dysenteriae, 64.02% for K. pneumoniae, and 63.10% for E. aerogenes when treated T. triquetra. Conclusion: Turbinaria triquetra extracted with methanol strongly affected the growth of the bacteria tested. Therefore, it is a potential source of natural antibacterial compounds.展开更多
Objective:To investigate the antimalarial and larvicidal activities of seaweeds against Plasmodium falciparum strain andAnopheles stephensi mosquito larva. Methods: The three different seaweeds,Jania rubens,Turbinaria...Objective:To investigate the antimalarial and larvicidal activities of seaweeds against Plasmodium falciparum strain andAnopheles stephensi mosquito larva. Methods: The three different seaweeds,Jania rubens,Turbinaria ornata (T. ornata) and Ulva fasciata were collected from Southeast India. The seaweeds were extracted using hexane and ethyl acetate solvents and evaluated for their mosquito-larvicidal and antiplasmodial property. The bioactive compounds of the seaweed extract were studied by Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. Results:The hexane extract ofT. ornata (HTO) has shown significant inhibitory effects at the IC50 value 22.98 μg/mL, when compared to the positive control, chloroquine (12.32 μg/mL). The larvicidal activity of HTO on the fourth instar larvae of Anopheles stephensi was found to be effective with the LC50 and LC90 values (μg/mL) of 1.38 and 3.34. The Fourier transform infrared spectroscopy analysis ofHTO revealed the presence of alkenes, aromatics, amides and amines groups, whereas in gas chromatography-mass spectrometry, 11 different bioactive components were recorded. The bioactive component, hentriacontane in the HTO might be responsible for the recorded antiplasmodial and larvicidal activities. Conclusions:The finding of the present study reveals that the constituents of T. ornata have new sources of antimalarial and larvicidal agents in future.展开更多
基金Supported by the funding under the Science and Engineering Research Council(SERC)Scheme(SR/FTP/CS-63/2007)from Department of Science and Technology.New Delhi.India
文摘Objective:To evaluate the antioxidant activities and total phenolic contents of brown seaweeds belonging to Turbinaria spp.[Turbinaria conoides(T.conoides)and Turbinaria ornata(T.ornata)collected from Gulf of Mannar of southeastern coast of India in various in vitro systems.Methods:The antioxidant activity was evaluated using different in vitro systems,viz.,I,I-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis-3 ethylbenzothiozoline-6-sulfonic acid diammonium salt(ABTS),H_20_2/H0 radical scavenging,Fe^(2+)ion chelating ability,and reducing potential.Folin-Ciocalteu method was used to determine the total phenolic content of the extracts,and the results were expressed as mg of gallic acid equivalents(GE)/g of the seaweed extracts.Thiobarbituric acid-reactive substances assay was employed to assess the ability of the seaweed extracts to inhibit lipid oxidation.Results:Ethyl acetate(EtOAc)fraction of T.conoides registered significantly higher phenolic content(105.97 mg GE/g)than that of T.ornata(69.63 mg GE/g).Significantly higher antioxidant potential as determined by DPPH(64.14%)radical scavenging activity was registered in EtOAc fraction of T.ornata.A higher ABTS(?)radical scavenging(IC_(50)3.16μg/mL),Fe^(2+)chelating(IC_(50)0.46 mg/mL),H_2O_2 scavenging(IC_(50)4.25 mg/mL),lipid peroxidation inhibitory(TBARS,IC_(50)0.21 mg/mL),and reducing abilities(IC_(50)52.67 mg/mL)(P<0.05)were realized in EtOAc fraction of T.ornata than other fractions.Conclusions:This study indicated the potential use of T.conoides and T.ornata as candidate species to be used as food supplements/functional foods to increase shelf-life of food items for human consumption,and nutraceuticals to deter deleterious free radical-induced life-threatening diseases.
文摘In this study,the biosorption of Malachite green(MG)onto Turbinaria conoides,brown marine algae,was studied with respect to initial pH,temperature,initial dye concentration,and sorbent dosage.The optimum initial pH and temperature values for MG removal were found to be 8.0 and 30℃,respectively.Sorbent dosage was found to strongly influence the removal of MG.Equilibrium studies were carried out to test the validity of the Langmuir(q_(max)=66.6 mg/g and b=0.526 mL mol/L)and the Freundlich(n=1.826 and K=3.751 mg/g)isotherms.The kinetic studies indicated the validity of the pseudo first-order and second-order equation.
基金Supported by Ministry of Education(MOE)of Malaysia and Research Intensive Faculty(RIF)of University of Technology MARA(UiTM)Malaysia[Grant No.600-RMI/DANA 5/3/RIF(185/2012)].
文摘Objective:To evaluate the in vivo acute toxicity and antioxidant activity of the marine endophytic fungus extract S2 isolated from Turbinaria conoides.Methods:Two doses(100 mg/kg and 400 mg/kg)of the S2 extract were administered to rats orally for acute toxicity and antioxidant test.The body weight,relative weight of six organs,haematological,biochemical and antioxidant properties were investigated on Day 14.Results:A single oral dose treatment did not cause any mortality or observable adverse effects in rats.No significant variations in the body and organ weights between the control and the treated groups were observed.Heamatological analysis and clinical blood chemistry also did not reveal any toxic effects of the extract.The total white blood cell count and haemoglobin levels were increased.The levels of total serum cholesterol in males treated with 100 and 400 mg/kg were significantly(P<0.05)decreased(1.28 and 1.34 mmol/L respectively)compared to control(1.55 mmol/L)rats.Pathologically,neither gross abnormalities nor histopathological changes were observed.This study showed strong evidence of the non-toxic effects of S2 extract.Furthermore the extract exhibited significant(P<0.05)antioxidant activity through increased levels of superoxide dismutase and glutathione peroxidase enzy mes in serum,liver and kidney.Conclusions:The research findings from the present study showed the potential of marine natural products particularly in Malaysia as a source of bioactive compounds.Marine endophytic fungi as a potential source of anticancer drugs with great potential as they are potent yet safe,thus deserving further extensive investigation.
基金Supported by the scheme of Centre with Potential for Excellence in Particular Area(CPEPA)[Grant No:F.No.8-2/2008(NS/PE)December 2011].
文摘Objective:To evaluate the antimicrobial capacity of fucoidan from the brown seaweed Turbinaria ornata against the marine ornamental fish bacterial pathogens.Methods:Fucoidan was isolated by the ethanol extraction method and the functional groups were identified by Fourier Transform Infrared Spectroscopy analysis.Subsequently,structural characterization was done by 1H NMR analysis.In vitro antibacterial activity of fucoidan was performed by the agar plate diffusion method and Minimum Inhibitory Concentration.Results:The characteristic C-0-S bending vibration of sulfate substituent the axial C-4 was observed at 839 cm^(-1).Characterisitic signal of the fucoidan was detected in a different ppm of 1H NMR analysis.The maximum antibacterial activity(16.23±0.11)mm was obtained for Vibrio parahaemolyticus and the minimum activity(5.1±0.24)mm was recorded for Yersinia enterocolitica.The Minimum Inhibitory Concentration value was recorded between 2.5 to 10 mg/mL to the respective pathogens.Conclusions:The present study proved that fucoidan possessed the significant antibacterial activity against the tested fish bacterial pathogens.It could be further used as a natural antibiotic in an aquaculture system to control the bacterial diseases.However,the present study suggested that the further in vivo study is required to get the better understanding of the mode of action of fucoidan.
基金Supported by the Department of Botany,Damietta University,and Damietta,Egypt as a part of MSc programme(Grant No.110/2012).
文摘Objective:To study ecological parameters and biochemical composition of brown seaweed,Turbinaria ornata(T.ornata)collected from Hurghada shores,Red Sea coast of Egypt during September,October and November,2015.Methods:T.ornata and its associated seaweeds were collected,identified and their abundances were estimated.Water of collection site was analyzed physicochemically as well as qualitative and quantitative analyses of phytoplankton.T.ornata was analyzed for protein,total carbohydrate,lipids,alginic acid,agar,pigments,minerals and heavy metals.Results:The results showed that macroalgal species recorded along Hurghada shores belong to Phaeophyta,Rhodophyta and Chlorophyta.At collection site,the moderate temperature,slight alkaline pH,low turbidity,high dissolved oxygen and valuable nutrient content of saline water exerted the massive growth of T.ornata with maximum abundance(24%)during October.The phytoplankton community was quite diverse with a maximum numbers of taxa(104.2×10^(8) cell/L)recorded during October.Analysis of T.ornata alga powder showed that high soluble carbohydrate(2.80±0.10 mg/g dry/weight)and chlorophyll c(0.0017±0.0001 mg/g fresh weight)contents were recorded during September;while high contents of protein(37.70±0.60 mg/g dry weight),lipids(3.10±0.06 mg/g dry weight),polysaccharides(agar and alginates),carotenoids(0.0160±0.0004 mg/g fresh weight),minerals and heavy metals were recorded during November.Conclusions:The study revealed that physicochemical analyses of water were varied slightly during the three months and suitable for the growth of T.ornata.It contains high amount of most biochemical constituents during October.
基金Supported by a grant from the Department of Botany and Microbiology,Damietta University,Damietta,Egypt as a part of MSc programme(Grant No.110/2012).
文摘Objective:To investigate the potential antibacterial activities of ethanol extracts of Turbinaria ornata(T.ornata),Oleic acid(OA)and palmitic acid(PA)extracted from T.ornata as well as mixtures of OA and PA(1:1)against some bacterial species.Methods:Brown seaweed T.ornata was collected from Hurghada shores,Red Sea coast of Egypt.OA and PA were extracted from T.ornata.Ethanol extracts of T.ornata,OA,PA and mixtures of these two fatty acids(1:1)were tested for their antibacterial activities against Escherichia coli,Bacillus cereus,Bacillus subtilis,Klebsiella pneumonia and Staphylococcus aureus by the disc diffusion method.Results:Ethanol extracts of T.ornata,OA,PA and mixtures of OA and PA(1:1)showed antibacterial activities that increased significantly(least significant difference at 0.05 level)with increasing concentrations against all tested bacteria.Different concentrations of ethanol extracts of T.ornata and extracted OA showed its highest activity against Bacillus subtilis,while PA and mixtures of PA and OA(1:1)showed its highest activity against Bacillus cereus.The maximum inhibition activities were shown for mixtures of OA and PA(1:1).Scanning electron microscope showed that mixtures of OA and PA(1:1)caused plasmolysis and reduction in cell size of Escherichia coli.Conclusions:Different concentrations of T.ornata and its fatty acids showed activities against all tested bacteria.Therefore,it is a potential source of natural antimicrobial compounds.
基金Supported by University Grants Commission,New Delhi.India
文摘Objective:To evaluate the antimicrobial activity of Turbinaria conoides(T.conoides).Padina gymnospora(P.gymnospora) and Sargassum tenerrimum against human bacterial and fungal pathogens.Methods:The antimicrobial activities of the extracts against various organisms were tested by using disc diffusion method.Results:The methanol extract showed the better result than the other extracts.Whereas,the strong antibacterial inhibition was noted in methanol extracts of P.gymnospora against Bacillus subtilus(26.33±1.86) and the mild inhibition of ethanol extracts from T.conoides against Klebsiella pneumoniae(2.33±0.51).Acetone extraction of P. gymnospora had strong antifungal inhibition against Cryptococcus neoformans(23.00±1.78), and acetone extract of T.conoides had mild inhibition against Aspergillus niger(3.00±0.89). Conclusions:The seven different solvent extracts of seaweeds used in the present study have shown significant bacterial action.Further,a detailed study on the principle compound in the seaweeds which is responsible for antimicrobial activity is still needed and it can be achieved by using advanced separation techniques.
文摘Introduction: Intestinal bacteria are exposed many external influences, including drugs, causing the emergence of strains resistant to the effects of antibiotics. Consequently, the discovery of new antibiotics that affect resistant strains is required. Marine algae offer a source of renewable natural compounds with antimicrobial effects. Therefore, the aim of this study was to detect some of these compounds and examine their impact on enteric bacteria. Methodology: Escherichia coli, Salmonella typhi, Shigella dysenteriae, Klebsiella pneumoniae, and Enterobacter aerogenes were tested with extracts of Turbinaria triquetra and Halimeda opuntia extracted with methanol, ethanol, petroleum ether, or dimethyl formamide solvents. We measured bacterial growth inhibition, the minimal inhibitory concentrations (MICs), and potassium leakage, and analyzed the bacterial cells with scanning electron microscopy and energy-dispersive X-ray spectroscopy. Results: The T. triquetra extract produced with methanol strongly affected the bacteria tested. When the results for T. triquetra and H. opuntia were compared with those of omacillin, the T. triquetra and H. opuntia extracts in most solvents were more effective than the antibiotic. Differences in the bacterial growth inhibition and MICs depended on the type of alga and the solvent used. At the end of the incubation period, potassium leakage had increased by 62.98% for E. coli, 61.24% for S. typhi, 61.32% for S. dysenteriae, 64.02% for K. pneumoniae, and 63.10% for E. aerogenes when treated T. triquetra. Conclusion: Turbinaria triquetra extracted with methanol strongly affected the growth of the bacteria tested. Therefore, it is a potential source of natural antibacterial compounds.
文摘Objective:To investigate the antimalarial and larvicidal activities of seaweeds against Plasmodium falciparum strain andAnopheles stephensi mosquito larva. Methods: The three different seaweeds,Jania rubens,Turbinaria ornata (T. ornata) and Ulva fasciata were collected from Southeast India. The seaweeds were extracted using hexane and ethyl acetate solvents and evaluated for their mosquito-larvicidal and antiplasmodial property. The bioactive compounds of the seaweed extract were studied by Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. Results:The hexane extract ofT. ornata (HTO) has shown significant inhibitory effects at the IC50 value 22.98 μg/mL, when compared to the positive control, chloroquine (12.32 μg/mL). The larvicidal activity of HTO on the fourth instar larvae of Anopheles stephensi was found to be effective with the LC50 and LC90 values (μg/mL) of 1.38 and 3.34. The Fourier transform infrared spectroscopy analysis ofHTO revealed the presence of alkenes, aromatics, amides and amines groups, whereas in gas chromatography-mass spectrometry, 11 different bioactive components were recorded. The bioactive component, hentriacontane in the HTO might be responsible for the recorded antiplasmodial and larvicidal activities. Conclusions:The finding of the present study reveals that the constituents of T. ornata have new sources of antimalarial and larvicidal agents in future.
