基于板蓝根及其混伪品叶绿体基因组的mini-barcode开发及其定性能力的研究

[目的]基于板蓝根及其混伪品的叶绿体基因组序列开发mini-barcode并验证其鉴定能力,为板蓝根的质量控制和科学评价提供方法。[方法]从GenBank数据库中下载板蓝根及其混伪品所有已发表的叶绿体基因组序列,导入PhyloSuite v1.2.1软件提取其共有蛋白编码基因并分别对齐,后续使用DnaSP 6软件进行核苷酸多态性分析以筛选出候选DNA mini-barcode区域。通过Geneious软件多序列比对,筛选高变区用于mini-barcode开发。针对候选条形码区域设计引物。采用试剂盒法提取板蓝根药材总DNA分别利用通用ITS2引物和该实验设计引物进行聚合酶链式反应(PCR)和测序。[结果]基于叶绿体基因组的核苷酸多态性分析结果,选择accD基因作为候选mini-barcode开发区域并设计了扩增引物。通过遗传距离和测序数据分析,该分子标记能被成功扩增并实现鉴定。[结论]开发了用于鉴定板蓝根及其混伪品的mini-barcode,为实现DNA降解的板蓝根药材及含板蓝根的中药制剂等混合样本的真伪鉴别奠定基础。

First record of abnormal body coloration in a rockfish Sebastes koreanus(Scorpaenoidei:Sebastidae)from coastal water of China based on morphological characteristics and DNA barcoding

The first record of abnormal body coloration in Sebastes koreanus Kim and Lee,1994,from the Yellow Sea of China,was documented based on morphological characteristics and DNA barcoding.The two rockfish specimens were collected from the coastal waters of Qingdao,China,and the whole body and all fins of them were red.Of the two red-colored rockfish,there were tiny deep red spots on each fin,2 red radial stripes behind and below the eyes and 1 large deep red blotch on the opercula,while the similar stripe and spot patterns are also present in the S.koreanus specimens with normal body coloration.The countable characteristics of the two specimens are in the range of the morphometry of S.koreanus.To further clarify the species identity and taxonomic status of the two specimens,DNA barcode analysis was carried out.The genetic distance between the red-colored rockfish and S.koreanus was 0,and the minimum net genetic distances between the red-colored rockfish and other Sebastes species except for S.koreanus were 3.0%,which exceeds the threshold of species delimitation.The phylogenetic analysis showed that the DNA barcoding sequences of the two red-colored rockfish clustered with the S.koreanus sequences.The above results of DNA barcode analysis also support that the two red-colored rockfish could be identified as the species of S.koreanus.The mechanism of color variation in S.koreanus is desirable for further research and the species could be an ideal model to study the color-driven speciation of the rockfishes.

关于Barcode自助借还机在高校图书馆应用满意度的实证研究——以江苏海洋大学图书馆为例

随着智慧图书馆概念的提出,Barcode(条形码)识别模式自助借还机作为一项新型的、日趋成熟的服务方式正广泛应用于图书馆。基于此,以Barcode自助借还机的使用情况为调研对象,了解读者和管理员使用后的满意度,以便发现自助借还机在使用过程中的各种利弊,并提出解决问题的有效途径。

DNA Barcoding of Insects and Its Direct Application for Plant Protection

The introduction of invasive insect pests across national borders has become a major concern in crop production. Accordingly, national plant protection organizations are challenge to reinforce their monitoring strategies, which are hampered by the weight and size of inspection equipment, as well as the taxonomic extensiveness of interrupted species. Moreover, some insect pests that impede farmer productivity and profitability are difficult for researchers to address on time due to a lack of appropriate plant protection measures. Farmers’ reliance on synthetic pesticides and biocontrol agents has resulted in major economic and environmental ramifications. DNA barcoding is a novel technology that has the potential to improve Integrated Pest Management decision-making, which is dependent on the ability to correctly identify pest and beneficial organisms. This is due to some natural traits such as phenology or pesticide susceptibility browbeaten by IPM strategies to avert pest establishment. Specifically, Deoxyribonucleic acid (DNA) sequence information was applied effectively for the identification of some micro-organisms. This technology, DNA barcoding, allows for the identification of insect species by using short, standardized gene sequences. DNA barcoding is basically based on repeatable and accessible technique that allows for the mechanisation or automation of species discrimination. This technique bridges the taxonomic bio-security gap and meets the International Plant Protection Convention diagnostic standards for insect identification. This review therefore discusses DNA barcoding as a technique for insect pests’ identification and its potential application for crop protection.

Analysis of the Authenticity of Stone Medicinal Plants by DNA Barcoding

The genus Pyrrosia belongs to the family Polypodiaceae and are medium-sized epiphytic ferns,where the dried leaves of Pyrrosia lingua,Pyrrosia sheareri,Pyrrosia lanceolata,and Pyrrosia calvata are commonly used in medicinal practice.In this study,the authenticity of the collected medicinal plant samples of Shiwei was identified with the help of DNA barcoding technology using the internal transcribed spacer 2(ITS2)as the identifying sequence.The experimental samples were analyzed using the basic local alignment search tool(BLAST)and the authenticity of the samples was further verified with the results of similarity comparison.The results proved that the sequences of the experimentally collected samples of Pyrrosia lingua,Pyrrosia sheareri,Pyrrosia lanceolata,and Pyrrosia calvata had a similarity of more than 97%when compared with the corresponding sequences that were uploaded on the Internet.

COI序列:影响动物分类学与生态学的DNA barcode

DNA barcode是一段特殊的、可用于物种鉴定的DNA序列。目前在动物中最常用的DNA barcode是细胞色素C氧化酶1号基因(COI)的部分序列。随着标准数据库的建立,基于COI基因的DNA barcode在动物分类学和生态学中得到了广泛应用。但是,采用COI基因作为DNA barcode所隐含的涉及线粒体的进化历史、遗传特性和物种成种时间的默认前提,并非完全成立,由此引发了许多问题。本文阐述了基于COI基因的DNA barcode对分类学和生态学的影响,目前存在的问题,以及可能的研究方向。

Barcode技术在ERP框架下生产过程管理中的应用研究

针对离散生产企业车间管理的黑箱作业,提出Barcode技术在企业资源计划(ERP)下生产过程管理中的应用框架,总结出Barcode技术在ERP下生产过程管理应用架构的三个主要技术,并介绍一种先进物料清单(BOM)设计和条码组合设计的思路。最后结合实施的多个案例分析此模式在离散制造业的应用。

Three newly recorded species of Acerentomata(Hexapoda:Protura) from China,with analysis of DNA barcodes

Three newly recorded species in the order Acerentomata in Protura from China are described:Filientomon duodecimsetosum Nakamura,2004,Verrucoentomon anatoli Shrubovych & Bernard,2012 and Verrucoentomon louisanne Shrubovych & Bernard,2012.The important morphological characters of Chinese specimens are described in detail.An updated key to Chinese Verrucoentomon species is provided.In addition,their DNA barcodes are sequenced and analyzed.

基于barcode4j的独立学院图书馆资产管理平台——以华南师范大学增城学院为例

文章阐述了Barcode4j的原理和开发方法,对平台的设计与开发进行了分析,提出了在校园网上开发图书馆资产网络管理平台的思路。

Barcode技术:增加输血安全性的作用

背景 错误的血液成分输入是最常见的输血相关的严重事故,这些频繁发生的错误原因包括病人的错误辨识。设计和方法 本研究评估Barcode病人辨识系统,包括手提电脑对采集血样的兼容性试验和血液管理的作用,和应用Barcode前后的作用。结果 基线检查显示尤其是病人辨识的实用性差。

Application of DNA Barcode Technology to the Identification of Exotic Mosquitoes at Ningbo Port

[Objective] The study aimed to solve the problem of morphological identi- fication difficulties and propose solutions for the identification of foreign mosquitoes that are difficult to identify. [Method] Based on the sequencing, alignment and anal- ysis of COl gene fragment, DNA barcode technology was used to identify 7 exotic mosquitoes, and the phylogenetic analysis was performed using MEGA6.0 and DNASTAR software. Then the morphological characteristics of the mosquitoes were reviewed. [Result[ These mosquitoes were Armigeres subalbatus, Culex gefidus, Anopheles gambiae, and Culiseta incidens. [Conclusion] DNA barcode technology is a useful supplement to the morphological classification method of mosquitoes.

Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes

Objective Poisonous plants are a deadly threat to public health in China. The traditional clinical diagnosis of the toxic plants is inefficient, fallible, and dependent upon experts. In this study, we tested the performance of DNA barcodes for identification of the most threatening poisonous plants in China. Methods Seventy-four accessions of 27 toxic plant species in 22 genera and 17 families were sampled and three DNA barcodes （motK, rbcL, and ITS） were amplified, sequenced and tested. Three methods, Blast, pairwise global alignment （PWG） distance, and Tree-Building were tested for discrimination power. Results The primer universality of all the three markers was high. Except in the case of ITS for Hemerocollis minor, the three barcodes were successfully generated from all the selected species. Among the three methods applied, Blast showed the lowest discrimination rate, whereas PWG Distance and Tree-Building methods were equally effective. The ITS barcode showed highest discrimination rates using the PWG Distance and Tree-Building methods. When the barcodes were combined, discrimination rates were increased for the Blast method.

An Improved Barcoded Oligonucleotide Primers-based Next-generation Sequencing Approach for Direct Identification of Viral Pathogens in Clinical Specimens

Objective To provide a feasible and cost-effective next-generation sequencing （NGS） method for accurate identification of viral pathogens in clinical specimens, because enormous limitations impede the clinical use of common NGS, such as high cost, complicated procedures, tremendous data analysis, and high background noise in clinical samples. Methods Viruses from cell culture materials or clinical specimens were identified following an improved NGS procedure： reduction of background noise by sample preprocessing, viral enrichment by barcoded oligonucleotide （random hexamer or non-ribosomal hexanucleotide） primer-based amplification, fragmentation-free library construction and sequencing of one-tube mixtures, as well as rapid data analysis using an in-house pipeline. Results NGS data demonstrated that both barcoded primer sets were useful to simultaneously capture multiple viral pathogens in cell culture materials or clinical specimens and verified that hexanucleotide primers captured as many viral sequences as hexamers did. Moreover, direct testing of clinical specimens using this improved hexanucleotide primer-based NGS approach provided further detailed genotypes of enteroviruses causing hand, foot, and mouth disease （HFMD） and identified other potential viruses or differentiated misdiagnosis events. Conclusion The improved barcoded oligonucleotide primer-based NGS approach is simplified, time saving, cost effective, and appropriate for direct identification of viral pathogens in clinical practice.

Barcode与RFID识别模式的自助借还系统比较分析

在论述Barcode与RFID识别模式图书馆自助借还系统原理的基础上,从成本、使用、管理和安全4方面分析两个模式现阶段应用于图书馆自助系统的优劣,并对上述两种识别模式的未来发展趋势进行了分析与展望。

Application of Evolutionary Encryption 2D Barcode Generation Technology in Agricultural Product Quality and Safety Traceability System

Two-dimensional(2D) barcode technology is an electronic tagging technology based on combination of computer and optical technology. It is an important way of information collection and input. 2D barcode technology has been widely used in various fields of logistics,production automation,and e-commerce,but it also has brought about a series of safety problems. Based on evolutionary encryption technology,this paper improved algorithm of traditional 2D barcode generation,to improve forgery- proof performance of 2D barcode. This algorithm is applied to agricultural products quality and safety traceability system and the results show that it is effective.

Applications of Three DNA Barcodes in Assorting Intertidal Red Macroalgal Flora in Qingdao, China

This study is part of the endeavor to construct a comprehensive DNA barcoding database for common seaweeds in China. Identifications of red seaweeds, which have simple morphology and anatomy, are sometimes difficult solely depending on morphological characteristics. In recent years, DNA barcode technique has become a more and more effective tool to help solve some of the taxonomic difficulties. Some DNA markers such as COI（cytochrome oxidase subunit I） are proposed as standardized DNA barcodes for all seaweed species. In this study, COI, UPA （universal plastid amplicon, domain V of 23S rRNA）, and ITS （nuclear internal transcribed spacer） were employed to analyze common species of intertidal red seaweeds in Qingdao （119.3°-121°E, 35.35°-37.09°N）. The applicability of using one or a few combined barcodes to identify red seaweed species was tested. The results indicated that COI is a sensitive marker at species level. However, not all the tested species gave PCR amplification products due to lack of the universal primers. The second barcode UPA had effective universal primers but needed to be tested for the effectiveness of resolving closely related species. More than one ITS sequence types were found in some species in this investigation, which might lead to confusion in further analysis. Therefore ITS sequence is not recommended as a universal barcode for seaweeds identification.

A WATERMARKING ALGORITHM BASED ON PERMUTATION AND 2-D BARCODE

This letter presents a method for digital image watermarking for copyright protection. This technique produces a watermarked image that closely retains the quality of the original host image while concurrently surviving various image processing operations such as lowpass/highpass filtering, lossy JPEG compression, and cropping. This image watermarking algorithm takes full advantage of permutation and 2-D barcode (PDF417). The actual watermark embedding in spatial domain is followed using permutated image for improving the resistance to image cropping. Much higher watermark robustness is obtainable via a simple forward error correction technique, which is the main feature of PDF417 codes. Additional features of this technique include the easy determination of the existence of the watermark and that the watermark verification procedure does not need the original host image. The experimental results demonstrate its effectiveness.

DNA Barcodes in Fig Cultivars (Ficus carica L.) Using ITS Regions of Ribosomal DNA, the psbA-trnH Spacer and the matK Coding Sequence

Molecular markers provide a useful method for genotype characterization and allow a high precision determination of the genetic relationship between cultivars and varieties. A system based on DNA sequences—which is known as DNA barcoding—will choose one or several standard loci which can be sequenced and compared to differentiate between species. In this research, the ITS, matK, and trnH-psbA sequences were evaluated for the molecular identification of seven F. carica genotypes, generating complete sequences for the first two loci, but unable to produce bidirectional sequences by using the trnH-psbA sequence. The ITS sequence presented the highest variation rates, while the phylogeny constructed with the matK sequence obtained the highest percentage of solved monophyletic groups. Through Pearson’s correlation analysis, it was possible to determine the existence of a significant correlation between the ITS region and psbA-trnH, and the matK and psbA-trnH sequences, but not between ITS and matK. The phylogenies constructed with the ITS + matK barcodes and ITS + matK + psbA-trnH presented the highest percentage for resolution. However, considering the cost efficiency and the facilitated recovery by using PCR, the matK + ITS combination is recommended.

COMPACTLY SUPPORTED NON-TENSOR PRODUCT FORM TWO-DIMENSION WAVELET FINITE ELEMENT

Some theorems of compactly supported non-tensor product form two-dimension Daubechies wavelet were analysed carefully. Compactly supported non-tensor product form two-dimension wavelet was constructed, then non-tensor product form two dimension wavelet finite element was used to solve the deflection problem of elastic thin plate. The error order was researched. A numerical example was given at last.

Crane flies(Diptera: Tipuloidea: Tipulidae) from Dayaoshan National Nature Reserve, China and analysis of DNA barcodes, with description of one new species in the genus Indotipula

This paper reports six species of crane flies from Dayaoshan National Nature Reserve, Guangxi Zhuang Autonomous Region, China, including one new species, Indotipula jinxiuensis sp. nov. The males of Pselliophora guangxiensis Yang Yang, 1988 and Holorusia basiflava Yang Yang, 1993 and female of Pselliophora xanthopimplina Enderlein, 1921 are redescribed and illustrated with new morphological characters. The females of P. guangxiensis and H. basiflava are described and illustrated for the first time. A key for separating known species of Indotipula Edwards, 1931 from China is provided. DNA barcodes of all species in this study are provided and analyzed.