一种改进的β淀粉样蛋白的电泳分离方法

目的 :建立一种简便、快捷的β淀粉样蛋白的电泳分离方法。 方法 :比较以往报道的各种低分子量蛋白的电泳分离方法 ,对电泳缓冲液系统、分离胶和浓缩胶的浓度、电泳程序等进行了优化。结果 :用改进的TwophasesTris -Trincine -SDS -PAGE可以简单、快速、清地分离 β淀粉样蛋白。结论 :新建立的TwophasesTris-Trincine

An explicit finite volume element method for solving characteristic level set equation on triangular grids

Level set methods are widely used for predicting evolutions of complex free surface topologies,such as the crystal and crack growth,bubbles and droplets deformation,spilling and breaking waves,and two-phase flow phenomena.This paper presents a characteristic level set equation which is derived from the two-dimensional level set equation by using the characteristic-based scheme.An explicit finite volume element method is developed to discretize the equation on triangular grids.Several examples are presented to demonstrate the performance of the proposed method for calculating interface evolutions in time.The proposed level set method is also coupled with the Navier-Stokes equations for two-phase immiscible incompressible flow analysis with surface tension.The Rayleigh-Taylor instability problem is used to test and evaluate the effectiveness of the proposed scheme.

Discharge Characteristics of Bubbles at Interface Between AIN Ceramic and FC-72 Liquid

In order to evaluate the insulation of two-phase immersion cooling in the HV power electronic package,the insulation degradation of the dielectric interface induced by bubbles is investigated.In this paper,a test strategy with 50 Hz unipolar DC and AC combined voltage for partial discharge(PD)at boiling interface of AlN ceramic is proposed.The insulation threshold of an AlN ceramic surface is acquired in several dielectric environments,such as air,FC-72 liquid(FC-72,a Fluorinert^(TM) from 3^(TM)),FC-72 vapor,and boiling state of FC72.This reveals the deterioration of boiling on the insulation of the surface immersed in the dielectric refrigerant.To investigate the mechanism of the PD feature at the boiling interface,the PD patterns of the unrestricted bubble and the accumulated bubble are acquired and contrastively analyzed.Combined with the feature of the back discharge and the bubble behavior,the charged vapor-ceramic interface is relatively stable due to the accumulated vapor layer.This stability of the charged vaporceramic interface is broken if the bubble is unrestricted.Besides,it is discovered that the vapor-liquid interface inside the bubble may be another charged interface,which can also trigger a back discharge.

Experimental study on dynamic behavior of mechanically pumped two-phase loop with a novel accumulator in simulated space environment

Mechanically pumped two-phase loop(MPTL)which is a prominent two-phase heat transfer technology presents a promising prospect in thermal control for space payload.However,transient behavior of MPTL caused by phase-change and heat sources load-on/off in simulated space environment is rarely reported.In the present study,one MPTL setup was designed and constructed,and experimentally studied.Particularly,a novel two-phase thermally-controlled accumulator integrated with passive cooling measure and three capillary structures was designed as the temperature-control device.Dynamic behavior of the start-up,temperature control,and temperature adjustment were monitored;meanwhile,thermodynamic behavior within the proposed accumulator,the operating behavior as well as the heat and mass transfer behavior between the main loop and the accumulator were revealed.The results show that the fluid management function of the capillary structures for the novel accumulator is verified.The working point of the MPTL system can be adjusted by changing the temperature control point of the accumulator and it is little influenced by external heat flux and heat sources on/off.Pressure-drop oscillations which are manifested as fluctuations of temperature and pressure can be observed after phase changing due to the compressible volume within the accumulator and the negative-slope portion of the internal pressure.