Two alkaloids tylophoridicine D (1) and tylophoridicine E (2) have been isolated from Tylophora atrofolliculata. The structures of the compounds were elucidated on the basis of spect -roscopic analysis.
Objective:To evaluate the antidiabetic potential of leaf extracts of Tylophora hirsuta(T.hirsuta).Methods:The methanolic and ethyl acetate extracts of T.hirsuta leaves were analyzed by high pressure liquid chromatogra...Objective:To evaluate the antidiabetic potential of leaf extracts of Tylophora hirsuta(T.hirsuta).Methods:The methanolic and ethyl acetate extracts of T.hirsuta leaves were analyzed by high pressure liquid chromatography.In vitro antioxidant activity was determined by ferric ion reduction,1,1-diphenyl-2-picrylhydrazyl,and hydrogen peroxide scavenging methods.In vitro alpha amylase(α-amylase)inhibitory activity of the plant extracts was assessed.In vivo antidiabetic potential was determined in alloxan-induced diabetic mice to assess glycated hemoglobin(HbA1c),oral glucose tolerance,serum amylase,lipid profile,fasting blood glucose,and body weight.Histopathological lesions of the pancreas,liver and kidney were observed.Oxidative stress biomarkers such as superoxide dismutase,catalase and peroxidase were also determined.Results:Quercetin,chlorogenic acid,p-coumaric acid,and m-coumaric acid were found in the plant extracts.The methanolic plant extract exhibited higher in vitro antioxidant activities than the ethyl acetate extract.Moreover,methanolic plant extract exhibited(83.90±1.56)%α-amylase inhibitory activity at 3.2 mg/mL concentration.Animal study showed that the methanolic extract of T.hirsuta improved the levels of fasting blood glucose,HbA1c,serumα-amylase,lipid profile,liver function biomarkers,and kidney functions of diabetic mice.Moreover,the methanolic extract ameliorated diabetes-related oxidative stress by increasing superoxide dismutase and catalase activities and decreasing peroxidase and malondialdehyde levels.Histopathological examination showed that the plant extract had improved the integrity of pancreatic islets of Langerhans and reduced the pathological lesions in the liver and kidney of diabetic mice.Conclusions:The methanolic extract of T.hirsuta exhibits pronounced antidiabetic activity in mice through reduction of oxidative stress.The plant extract has several natural antioxidants such as phenolic acids.T.hirsuta extract could serve as a nutraceutical for managing diabetes mellitus.展开更多
Our research work demonstrates the single bead alginate-encapsulation, interim storing and conversion of Tylophora indica(Burm. Fil.) Merrill.Most effective encapsulation of in vitro nodal segments [(4 ± 1) mm lo...Our research work demonstrates the single bead alginate-encapsulation, interim storing and conversion of Tylophora indica(Burm. Fil.) Merrill.Most effective encapsulation of in vitro nodal segments [(4 ± 1) mm long], ensuing in sphere-shaped artificial seeds of similar morphology, was achieved through 75 mmol·L^(-1)calcium chloride(CaCl_2 · 2H_2O) plus 3%(w/v) Na-alginate with 93.3% conversion frequency. The earliest conversion(within 7 days of incubation) of artificial seeds occurred in half-strength liquid Murashige and Skoog medium. Among the three different temperature regimes [(5 ± 1) °C,(15 ± 1) °C, and(25 ± 1) °C], storage of artificial seeds at(15 ± 1) °C executed the highest frequency of conversion(90%) after 15 days of storage. Lengthier storage significantly reduced the conversion frequency of artificial seeds irrespective of storage temperature. Nevertheless, the conversion frequency after 30 days of storage at(15 ± 1) °C was recorded at 70% without further decline even following45 days of storage, which evidently suggests that lower temperature(15 ± 1) °C is apt for storage and subsequent conversion of T. indica artificial seeds. The present protocol could be expedient for short-term storing and swapping of T. indica germplasms between national and international laboratories.展开更多
基金The financial support of the Nationat Key Basic R&D(973)Programe(No.G1998-05l120)are grafefully acknowleged
文摘Two alkaloids tylophoridicine D (1) and tylophoridicine E (2) have been isolated from Tylophora atrofolliculata. The structures of the compounds were elucidated on the basis of spect -roscopic analysis.
文摘Objective:To evaluate the antidiabetic potential of leaf extracts of Tylophora hirsuta(T.hirsuta).Methods:The methanolic and ethyl acetate extracts of T.hirsuta leaves were analyzed by high pressure liquid chromatography.In vitro antioxidant activity was determined by ferric ion reduction,1,1-diphenyl-2-picrylhydrazyl,and hydrogen peroxide scavenging methods.In vitro alpha amylase(α-amylase)inhibitory activity of the plant extracts was assessed.In vivo antidiabetic potential was determined in alloxan-induced diabetic mice to assess glycated hemoglobin(HbA1c),oral glucose tolerance,serum amylase,lipid profile,fasting blood glucose,and body weight.Histopathological lesions of the pancreas,liver and kidney were observed.Oxidative stress biomarkers such as superoxide dismutase,catalase and peroxidase were also determined.Results:Quercetin,chlorogenic acid,p-coumaric acid,and m-coumaric acid were found in the plant extracts.The methanolic plant extract exhibited higher in vitro antioxidant activities than the ethyl acetate extract.Moreover,methanolic plant extract exhibited(83.90±1.56)%α-amylase inhibitory activity at 3.2 mg/mL concentration.Animal study showed that the methanolic extract of T.hirsuta improved the levels of fasting blood glucose,HbA1c,serumα-amylase,lipid profile,liver function biomarkers,and kidney functions of diabetic mice.Moreover,the methanolic extract ameliorated diabetes-related oxidative stress by increasing superoxide dismutase and catalase activities and decreasing peroxidase and malondialdehyde levels.Histopathological examination showed that the plant extract had improved the integrity of pancreatic islets of Langerhans and reduced the pathological lesions in the liver and kidney of diabetic mice.Conclusions:The methanolic extract of T.hirsuta exhibits pronounced antidiabetic activity in mice through reduction of oxidative stress.The plant extract has several natural antioxidants such as phenolic acids.T.hirsuta extract could serve as a nutraceutical for managing diabetes mellitus.
文摘Our research work demonstrates the single bead alginate-encapsulation, interim storing and conversion of Tylophora indica(Burm. Fil.) Merrill.Most effective encapsulation of in vitro nodal segments [(4 ± 1) mm long], ensuing in sphere-shaped artificial seeds of similar morphology, was achieved through 75 mmol·L^(-1)calcium chloride(CaCl_2 · 2H_2O) plus 3%(w/v) Na-alginate with 93.3% conversion frequency. The earliest conversion(within 7 days of incubation) of artificial seeds occurred in half-strength liquid Murashige and Skoog medium. Among the three different temperature regimes [(5 ± 1) °C,(15 ± 1) °C, and(25 ± 1) °C], storage of artificial seeds at(15 ± 1) °C executed the highest frequency of conversion(90%) after 15 days of storage. Lengthier storage significantly reduced the conversion frequency of artificial seeds irrespective of storage temperature. Nevertheless, the conversion frequency after 30 days of storage at(15 ± 1) °C was recorded at 70% without further decline even following45 days of storage, which evidently suggests that lower temperature(15 ± 1) °C is apt for storage and subsequent conversion of T. indica artificial seeds. The present protocol could be expedient for short-term storing and swapping of T. indica germplasms between national and international laboratories.
