TypeⅥSecretion System(T6SS)plays significant roles in microbial activities via injecting effectors into adjacent cells or environments.T6SS increasingly gained attention due to its important influence on pathogenesis...TypeⅥSecretion System(T6SS)plays significant roles in microbial activities via injecting effectors into adjacent cells or environments.T6SS increasingly gained attention due to its important influence on pathogenesis,microbial competition,etc.T6SS-associated research is explosively expanding on numerous grounds that call for an efficient resource.The SecReT6 version3 provides comprehensive information on T6SS and the interactions between T6SS and T6SS-related proteins such as T6SS regulators and T6SS effectors.To assist T6SS researches like microbial competition and regulatory mechanisms,SecReT6 v3developed online tools for detection and analysis of T6SS and T6SS-related proteins and estimation of T6SS-dependent killing risk.We have identified a novel T6SS regulator and T6SS-dependent killing capacity in Acinetobacter baumannii clinical isolates with the aid of SecReT6 v3.17,212 T6SSs and plentiful T6SS-related proteins in 26,573 bacterial complete genomes were also detected,analyzed and incorporated into the database.The database is freely available at https://bioinfo-mml.sjtu.edu.cn/SecReT6/.展开更多
目的:探讨掌骨嫁接结合软组织重建术在WasselⅥ型复拇畸形整复中的应用效果。方法:以2014年1月-2018年12月笔者医院收治的20例(20拇)WasselⅥ型复拇畸形患儿为研究对象,均施掌骨嫁接结合软组织重建术,术后至少随访12个月。采用体格检查...目的:探讨掌骨嫁接结合软组织重建术在WasselⅥ型复拇畸形整复中的应用效果。方法:以2014年1月-2018年12月笔者医院收治的20例(20拇)WasselⅥ型复拇畸形患儿为研究对象,均施掌骨嫁接结合软组织重建术,术后至少随访12个月。采用体格检查、X线及日本手外科协会(Japanese Society for Surgery of the Hand,JSSH)评分表对患儿重建拇指的外观、功能及稳定性进行评估。结果:20例患儿术后均获得完整随访,随访时间15~42(32.16±8.33)个月,截骨端均在术后6周得到理想愈合;掌骨长度占健侧的比例为(90.54±2.13)%,2例患儿出现10°的指间关节主动伸直功能受限,2例患儿出现17°的掌指关节桡偏;末次随访时的JSSH评分为(20.08±0.40)分,优15例,良5例,优良率为100%。结论:掌骨嫁接结合软组织重建术在WasselⅥ型复拇畸形整复中具有理想的手术效果,可以作为该类复拇畸形的术式选择。展开更多
The type Ⅵ secretion system(T6SS)is a widespread protein secretion apparatus deployed by many Gram-negative bacterial species to interact with competitor bacteria,host organisms,and the environment.Yersinia pseudotub...The type Ⅵ secretion system(T6SS)is a widespread protein secretion apparatus deployed by many Gram-negative bacterial species to interact with competitor bacteria,host organisms,and the environment.Yersinia pseudotuber-culosis T6SS4 was recently reported to be involved in manganese acquisition;however,the underlying regulatory mechanism still remains unclear.In this study,we discovered that T6SS4 is regulated by ferric uptake regulator(Fur)in response to manganese ions(Mn^(2+)),and this negative regulation of Fur was proceeded by specifically recogniz-ing the promoter region of T6SS4 in Y.pseudotuberculosis.Furthermore,T6SS4 is induced by low Mn^(2+)and oxidative stress conditions via Fur,acting as a Mn^(2+)-responsive transcriptional regulator to maintain intracellular manganese homeostasis,which plays important role in the transport of Mn^(2+)for survival under oxidative stress.Our results pro-vide evidence that T6SS4 can enhance the oxidative stress resistance and virulence for Y.pseudotuberculosis.This study provides new insights into the regulation of T6SS4 via the Mn^(2+)-dependent transcriptional regulator Fur,and expands our knowledge of the regulatory mechanisms and functions of T6SS from Y.pseudotuberculosis.展开更多
基金supported by the Science and Technology Commission of Shanghai Municipality(19430750600,19JC1413000)the National Natural Science Foundation of China(31670074)+1 种基金the Medical Excellence Award Funded by the Creative Research Development Grant from the First Affiliated Hospital of Guangxi Medical University(XK2019025)the Science Fund of the Republic of Serbia(7750294,q-bio BDS)。
文摘TypeⅥSecretion System(T6SS)plays significant roles in microbial activities via injecting effectors into adjacent cells or environments.T6SS increasingly gained attention due to its important influence on pathogenesis,microbial competition,etc.T6SS-associated research is explosively expanding on numerous grounds that call for an efficient resource.The SecReT6 version3 provides comprehensive information on T6SS and the interactions between T6SS and T6SS-related proteins such as T6SS regulators and T6SS effectors.To assist T6SS researches like microbial competition and regulatory mechanisms,SecReT6 v3developed online tools for detection and analysis of T6SS and T6SS-related proteins and estimation of T6SS-dependent killing risk.We have identified a novel T6SS regulator and T6SS-dependent killing capacity in Acinetobacter baumannii clinical isolates with the aid of SecReT6 v3.17,212 T6SSs and plentiful T6SS-related proteins in 26,573 bacterial complete genomes were also detected,analyzed and incorporated into the database.The database is freely available at https://bioinfo-mml.sjtu.edu.cn/SecReT6/.
文摘目的:探讨掌骨嫁接结合软组织重建术在WasselⅥ型复拇畸形整复中的应用效果。方法:以2014年1月-2018年12月笔者医院收治的20例(20拇)WasselⅥ型复拇畸形患儿为研究对象,均施掌骨嫁接结合软组织重建术,术后至少随访12个月。采用体格检查、X线及日本手外科协会(Japanese Society for Surgery of the Hand,JSSH)评分表对患儿重建拇指的外观、功能及稳定性进行评估。结果:20例患儿术后均获得完整随访,随访时间15~42(32.16±8.33)个月,截骨端均在术后6周得到理想愈合;掌骨长度占健侧的比例为(90.54±2.13)%,2例患儿出现10°的指间关节主动伸直功能受限,2例患儿出现17°的掌指关节桡偏;末次随访时的JSSH评分为(20.08±0.40)分,优15例,良5例,优良率为100%。结论:掌骨嫁接结合软组织重建术在WasselⅥ型复拇畸形整复中具有理想的手术效果,可以作为该类复拇畸形的术式选择。
基金supported by grants of the National Key R&D Program of China(Grants 2018YFA0901200)National Natural Science Foundation of China(Grants 31725003,31970114,32100034,and 32100149)+2 种基金Scientific Startup Foundation for Doctors of Northwest A and F University(Z1090122001 and Z1090122002)the China Postdoctoral Science Foundation(Grant 2020 M673501)Young Talent Support Program of Shaanxi Province University(20220206).
文摘The type Ⅵ secretion system(T6SS)is a widespread protein secretion apparatus deployed by many Gram-negative bacterial species to interact with competitor bacteria,host organisms,and the environment.Yersinia pseudotuber-culosis T6SS4 was recently reported to be involved in manganese acquisition;however,the underlying regulatory mechanism still remains unclear.In this study,we discovered that T6SS4 is regulated by ferric uptake regulator(Fur)in response to manganese ions(Mn^(2+)),and this negative regulation of Fur was proceeded by specifically recogniz-ing the promoter region of T6SS4 in Y.pseudotuberculosis.Furthermore,T6SS4 is induced by low Mn^(2+)and oxidative stress conditions via Fur,acting as a Mn^(2+)-responsive transcriptional regulator to maintain intracellular manganese homeostasis,which plays important role in the transport of Mn^(2+)for survival under oxidative stress.Our results pro-vide evidence that T6SS4 can enhance the oxidative stress resistance and virulence for Y.pseudotuberculosis.This study provides new insights into the regulation of T6SS4 via the Mn^(2+)-dependent transcriptional regulator Fur,and expands our knowledge of the regulatory mechanisms and functions of T6SS from Y.pseudotuberculosis.
