Objective: To select a target molecule associated with invasive potential in PC-3M cell. Methods: Cell subclones were isolated from PC-3M cell line with the method of limited dilution and their invasive ability charac...Objective: To select a target molecule associated with invasive potential in PC-3M cell. Methods: Cell subclones were isolated from PC-3M cell line with the method of limited dilution and their invasive ability characterized by monolayer invasion assay. The expression of u-PAR in the cell subclones at mRNA and protein levels was assayed respectively by non-competitive quantitative RT-PCR and immunohistochemical assay. Results: The expression level of u-PAR in highly invasive cell subclones was higher than that of lower invasive subclones. Conclusion: The higher expression level of u-PAR is associated with the relative strong invasive ability of PC-3M subclones. It is indicated that the u-PAR might be a promising target molecule for inhibiting invasion of highly invasive PC-3M cell subclones.展开更多
This paper aims to develop a new robust U-type test for high dimensional regression coefficients using the estimated U-statistic of order two and refitted cross-validation error variance estimation. It is proved that ...This paper aims to develop a new robust U-type test for high dimensional regression coefficients using the estimated U-statistic of order two and refitted cross-validation error variance estimation. It is proved that the limiting null distribution of the proposed new test is normal under two kinds of ordinary models.We further study the local power of the proposed test and compare with other competitive tests for high dimensional data. The idea of refitted cross-validation approach is utilized to reduce the bias of sample variance in the estimation of the test statistic. Our theoretical results indicate that the proposed test can have even more substantial power gain than the test by Zhong and Chen(2011) when testing a hypothesis with outlying observations and heavy tailed distributions. We assess the finite-sample performance of the proposed test by examining its size and power via Monte Carlo studies. We also illustrate the application of the proposed test by an empirical analysis of a real data example.展开更多
In the presence of applied magnetic fields H such that |lnε|《H《1/ε2,the author evaluates the minimal Ginzburg-Landau energy with discontinuous constraint.Its expression is analogous to the work of Sandier and Serf...In the presence of applied magnetic fields H such that |lnε|《H《1/ε2,the author evaluates the minimal Ginzburg-Landau energy with discontinuous constraint.Its expression is analogous to the work of Sandier and Serfaty.展开更多
文摘Objective: To select a target molecule associated with invasive potential in PC-3M cell. Methods: Cell subclones were isolated from PC-3M cell line with the method of limited dilution and their invasive ability characterized by monolayer invasion assay. The expression of u-PAR in the cell subclones at mRNA and protein levels was assayed respectively by non-competitive quantitative RT-PCR and immunohistochemical assay. Results: The expression level of u-PAR in highly invasive cell subclones was higher than that of lower invasive subclones. Conclusion: The higher expression level of u-PAR is associated with the relative strong invasive ability of PC-3M subclones. It is indicated that the u-PAR might be a promising target molecule for inhibiting invasion of highly invasive PC-3M cell subclones.
基金supported by National Natural Science Foundation of China (Grant Nos. 11071022, 11231010 and 11471223)Beijing Center for Mathematics and Information Interdisciplinary ScienceKey Project of Beijing Municipal Educational Commission (Grant No. KZ201410028030)
文摘This paper aims to develop a new robust U-type test for high dimensional regression coefficients using the estimated U-statistic of order two and refitted cross-validation error variance estimation. It is proved that the limiting null distribution of the proposed new test is normal under two kinds of ordinary models.We further study the local power of the proposed test and compare with other competitive tests for high dimensional data. The idea of refitted cross-validation approach is utilized to reduce the bias of sample variance in the estimation of the test statistic. Our theoretical results indicate that the proposed test can have even more substantial power gain than the test by Zhong and Chen(2011) when testing a hypothesis with outlying observations and heavy tailed distributions. We assess the finite-sample performance of the proposed test by examining its size and power via Monte Carlo studies. We also illustrate the application of the proposed test by an empirical analysis of a real data example.
文摘In the presence of applied magnetic fields H such that |lnε|《H《1/ε2,the author evaluates the minimal Ginzburg-Landau energy with discontinuous constraint.Its expression is analogous to the work of Sandier and Serfaty.
