Objective: To investigate U parvum (previously Ureaplasmaurealyticum biovar 1) and U urealyticum (previouslyUreaplasma urealyticum biovar 2) and their subtypes andserovars in urine specimens of pregnant women. Methods...Objective: To investigate U parvum (previously Ureaplasmaurealyticum biovar 1) and U urealyticum (previouslyUreaplasma urealyticum biovar 2) and their subtypes andserovars in urine specimens of pregnant women. Methods: After collecting 151 specimens and inoculatingbroth, all broth culture positive (urease positive) specimenswere amplified, species were identified and subtyped by usinggeneral primers, species-specific, and type-specific primerstargeting the multiple banded antigen (MBA) gene sequence. Results: U parvum was identified in 58 of 151 specimens andU. urealyticum in 18 (both were present in 5, and neither werefound in 6). Serovars 3, 1, and 6 were the most commonamong U parvum isolates and subtypes l and 3 were the mostcommon among U urealyticum. Multiple serovars amongclinical isolates were found. Conclusion: This PCR-based typing system could facilitatestudies of the relationship between individual ureaplasmaspecies or subtypes and human diseases.展开更多
文摘Objective: To investigate U parvum (previously Ureaplasmaurealyticum biovar 1) and U urealyticum (previouslyUreaplasma urealyticum biovar 2) and their subtypes andserovars in urine specimens of pregnant women. Methods: After collecting 151 specimens and inoculatingbroth, all broth culture positive (urease positive) specimenswere amplified, species were identified and subtyped by usinggeneral primers, species-specific, and type-specific primerstargeting the multiple banded antigen (MBA) gene sequence. Results: U parvum was identified in 58 of 151 specimens andU. urealyticum in 18 (both were present in 5, and neither werefound in 6). Serovars 3, 1, and 6 were the most commonamong U parvum isolates and subtypes l and 3 were the mostcommon among U urealyticum. Multiple serovars amongclinical isolates were found. Conclusion: This PCR-based typing system could facilitatestudies of the relationship between individual ureaplasmaspecies or subtypes and human diseases.
