目的：利用UHPLC-DAD建立黔产苗药百尾参的指纹图谱,为评价其质量提供理论依据。方法：以Agilent Eclipse ZORBAX Plus C18（100 mm×2.1 mm,1.8μm）为色谱柱,0.1%甲酸乙腈（A）-0.1%甲酸水（B）为流动相系统梯度洗脱,流速为0.3 m ...目的：利用UHPLC-DAD建立黔产苗药百尾参的指纹图谱,为评价其质量提供理论依据。方法：以Agilent Eclipse ZORBAX Plus C18（100 mm×2.1 mm,1.8μm）为色谱柱,0.1%甲酸乙腈（A）-0.1%甲酸水（B）为流动相系统梯度洗脱,流速为0.3 m L/min,检测波长290 nm,柱温40℃,进样量3μL。指纹图谱进行了相似度评价和主成分分析。结果：建立了黔产苗药百尾参的UHPLC-DAD指纹图谱,标定了12个共有峰,确认了其中3个成分。30批药材相似度结果为0.766-0.994,主成分分析提示1号峰和12号峰在指纹图谱中具有代表意义。结论：该方法准确、可靠,可用于百尾参药材的鉴别和质量控制。展开更多
Caffeine is present in products marketed for weight loss, with the purpose of increasing thermogenesis and lipid metabolism. The dosage declared by the product manufacturer, or even its presence, is not always correct...Caffeine is present in products marketed for weight loss, with the purpose of increasing thermogenesis and lipid metabolism. The dosage declared by the product manufacturer, or even its presence, is not always correctly described on the label. This work aimed to investigate the undeclared synthetic caffeine in weight loss formulations by a high-performance liquid chromatography with diode array detection(HPLC-DAD) method. From one hundred products purchased through Brazilian e-commerce, seventeen contained caffeine, either naturally or synthetically added to formulation. The caffeine-containing samples were confirmed by an ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS) method, and adulteration was clearly proven in five products. The content highest caffeine contained 448.8 mg per dose. Other irregularities were also found; nevertheless, the most serious was the addition of synthetic drugs without asking the consumers. Additional drugs expose the consumer to more possible side-effects as well as deleterious drug interactions. Intentional adulteration with any unlabeled substance is typically motivated by a desire to increase or alter the claimed effect of the marketed product to gain a commercial advantage.展开更多
文摘目的：利用UHPLC-DAD建立黔产苗药百尾参的指纹图谱,为评价其质量提供理论依据。方法：以Agilent Eclipse ZORBAX Plus C18（100 mm×2.1 mm,1.8μm）为色谱柱,0.1%甲酸乙腈（A）-0.1%甲酸水（B）为流动相系统梯度洗脱,流速为0.3 m L/min,检测波长290 nm,柱温40℃,进样量3μL。指纹图谱进行了相似度评价和主成分分析。结果：建立了黔产苗药百尾参的UHPLC-DAD指纹图谱,标定了12个共有峰,确认了其中3个成分。30批药材相似度结果为0.766-0.994,主成分分析提示1号峰和12号峰在指纹图谱中具有代表意义。结论：该方法准确、可靠,可用于百尾参药材的鉴别和质量控制。
文摘Caffeine is present in products marketed for weight loss, with the purpose of increasing thermogenesis and lipid metabolism. The dosage declared by the product manufacturer, or even its presence, is not always correctly described on the label. This work aimed to investigate the undeclared synthetic caffeine in weight loss formulations by a high-performance liquid chromatography with diode array detection(HPLC-DAD) method. From one hundred products purchased through Brazilian e-commerce, seventeen contained caffeine, either naturally or synthetically added to formulation. The caffeine-containing samples were confirmed by an ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS) method, and adulteration was clearly proven in five products. The content highest caffeine contained 448.8 mg per dose. Other irregularities were also found; nevertheless, the most serious was the addition of synthetic drugs without asking the consumers. Additional drugs expose the consumer to more possible side-effects as well as deleterious drug interactions. Intentional adulteration with any unlabeled substance is typically motivated by a desire to increase or alter the claimed effect of the marketed product to gain a commercial advantage.
