基于UHPLC-MS技术的NSCLC血浆生物学标志物的筛选及鉴定研究

目的研究UHPLC-MS技术的NSCLC血浆生物学标志物的筛选及鉴定。方法本研究纳入符合非小细胞肺癌临床诊断标准的60例NSCLC患者,随机分为两组,接受靶向治疗(TACE)的非小细胞肺癌患者为鉴别诊断组,未接受靶向治疗的NSCLC患者为正常对照组。结果在60例NSCLC患者中,通过LC-MS/MS技术共检测到552个生物标志物。在其中17个生物标志物中,有8个出现在已知的EGFR突变患者中,另7个出现在ALK融合患者中,6个出现在HER2突变患者中。此外,NSCLC患者血浆中含有丰富的非编码RNA(miRNA),包括58个miRNA和23个mRNA。结论本研究中,采用LC-MS/MS技术对NSCLC患者血浆进行分析,可快速、高效地获得血浆样品中的生物学标志物。这些标志物可以作为NSCLC诊断的潜在生物学标志物,并且可以用于预测肿瘤进展和不良预后。

UHPLC-MS/MS同时测定清感童饮中6种有效成分在大鼠体内血药浓度及药代动力学研究

[目的]本实验旨在研究清感童饮活性成分在大鼠血浆中的药代动力学特征。[方法]实验建立大鼠血浆中甘草次酸、木犀草素、芹菜素、芍药苷、连翘酯苷A、连翘苷等6种活性成分的超高效液相色谱-串联质谱(UHPLC-MS/MS)检测方法,考察大鼠灌胃给药清感童饮后,6种成分在大鼠体内的药代动力学特征。采用WELCN Ultimate XB-C_(18)色谱柱(2.1 mm×100 mm,3μm)分离;流动相为0.1%甲酸水(A)-乙腈(B);梯度洗脱;进样量3μL;流速为0.2 mL/min;柱温40℃;在多反应监测模式(MRM)下正、负离子同时测定。[结果]结果显示,大鼠血浆样品中各成分的线性关系均良好(r>0.99),其日内、日间精密度、准确度、稳定性、基质效应以及提取回收率均符合生物样品的分析要求。药动学参数结果显示,各成分的t_(1/2z)为1.18~34.12 h,T_(max)为0.44~19.33 h,C_(max)为9.00~318.09μg/L,AUC_(0~t)为12.40~15 969.32 h·μg/L,MRT_(0~t)为2.23~23.19 h。[结论]本实验所建立的检测方法快速、准确、重复性好,可适用于清感童饮中甘草次酸、木犀草素、芹菜素、芍药苷、连翘酯苷A、连翘苷在大鼠血浆中的药动学研究。

基于网络药理学结合UHPLC-MS/MS探讨清感童饮的抗炎作用

目的 基于血清药理学和网络药理学探究清感童饮的体外抗炎作用。方法 采用血清药理学评价方法,验证清感童饮含药血清对脂多糖(LPS)诱导的小鼠巨噬细胞(RAW264.7)释放一氧化氮(NO)、细胞坏死因子α(TNF-α)、白细胞介素6(IL-6)的影响。利用液质联用(UHPLC-MS/MS)技术对清感童饮指标性成分进行含量测定,并根据这些成分进行网络药理学分析,对清感童饮有效成分发挥抗炎作用的潜在靶点及作用通路进行预测。结果 细胞实验证明清感童饮可明显降低NO、TNF-α、IL-6水平(P<0.05,P<0.01,P<0.001)。清感童饮中含量较高的成分为连翘酯苷A、牛蒡苷、绿原酸、野黄芩苷、没食子酸、迷迭香酸、芍药内酯苷、连翘苷。清感童饮中17个活性成分与炎症的交集靶点共215个,主要涉及ALB、VEGFA、IL-6、TNF-α等31个核心靶点,调节AGE-RAGE、PI3K-Akt、MAPK信号通路等多种通路发挥抗炎作用。结论 清感童饮具有多成分-多靶点发挥抗炎作用的特点。

UHPLC-MS/MS法测定酱油中的焦谷氨酸

建立了测定酱油中焦谷氨酸的超高效液相色谱-串联质谱(UHPLC-MS/MS)分析方法。采用水直接提取样品中的焦谷氨酸,并适当稀释,将供试液用安捷伦InfinityLab Poroshell 120 Aq-C18(Φ4.6 mm×50 mm, 2.7μm)进行色谱分离,使用0.1%甲酸水溶液-0.1%甲酸乙腈溶液流动相体系梯度洗脱。电喷雾离子源正离子(ESI+)模式,多反应监测(MRM)模式进行测定。实验结果显示,焦谷氨酸的线性范围为0.025~2.5μg/mL,线性关系良好,相关系数(R^(2))为0.9992,检出限(LOD)和定量限(LOQ)为0.45 mg/kg和1.5 mg/kg。加标样品的回收率在80.7%~101.1%之间,精密度(RSD)(n=6)为2.4%~8.3%。该方法简单高效,灵敏准确,能应用于酱油中焦谷氨酸含量的定量检测。

基于UHPLC-MS/MS、分子对接及细胞实验探讨健脾益气方对肝癌PD-L1和PD-1表达的影响

目的采用超高效液相色谱-串联质谱(UHPLC-MS/MS)、分子对接和体外细胞实验探讨健脾益气方对肝癌细胞的影响。方法制备健脾益气方甲醇水溶液并采用UHPLC-MS/MS法分析鉴定中药活性成分。利用Schrodinger软件对鉴定出的所有活性成分及信号通路核心靶点白介素6(IL-6)、糖蛋白130(gp130)、酪氨酸激酶1(JAK1)、酪氨酸激酶2(JAK2)、信号转导蛋白及转录激活子3(STAT3)、程序性细胞死亡蛋白-1(PD-1)和程序性细胞死亡配体1(PD-L1)分别进行分子对接;并用Pymol软件将各靶标蛋白结合能最低的活性成分对接模式进行可视化。采用健脾益气方含药血清干预人肝癌细胞株HepG2进行体外实验验证,将HepG2细胞分为空白组,模型组,健脾益气方低、中、高剂量组(5.25、10.5、21 g/kg),STAT3抑制组(C188-9,4μmol/L)和gp130抑制组(SC144,10μmol/L)。除空白组外,模型组加入IL-6(50 ng/mL),各给药组加入IL-6和相应药物进行干预。采用CCK-8法、Transwell培养小室法、Annexin V+PI双染色法分别检测各组细胞存活率、侵袭数和凋亡率,ELISA法检测各组细胞培养液中IL-6水平,Western blot法检测各组细胞中gp130、磷酸化JAK1(p-JAK1)、磷酸化JAK2(p-JAK2)、磷酸化STAT3(p-STAT3)、PD-1和PD-L1蛋白表达。结果UHPLC-MS/MS鉴定出健脾益气方113个活性成分。分子对接结果显示,健脾益气方中有多个活性成分与IL-6、gp130、JAK1、JAK2、STAT3、PD-1和PD-L1靶点蛋白均分别存在分子结合位点且有较低结合能,均小于-5.0 kcal/mol。体外细胞实验显示,与空白组比较,模型组细胞侵袭数升高(P<0.01),细胞凋亡率则降低(P<0.01);培养液中IL-6水平升高(P<0.01);gp130、p-JAK1、p-JAK2、p-STAT3、PD-1和PD-L1蛋白表达均升高(P<0.01)。与模型组比较,除低剂量健脾益气方含药血清对细胞侵袭数的抑制和对细胞凋亡率的影响无明显变化外(P>0.05),其它各药物干预组均可抑制模型细胞的增殖与侵袭能力,促进细胞凋亡(P<0.01);各药物干预组均可降低模型细胞培养液中IL-6水平(P<0.01);并降低细胞中gp130、p-JAK1、p-JAK2、p-STAT3、PD-1和PD-L1蛋白表达(P<0.01)。结论健脾益气方可通过多成分调控肝癌细胞IL-6/STAT3炎症信号通路及其介导的PD-L1和固有PD-1的表达,抑制肝癌细胞增殖侵袭,促进细胞凋亡。

Development and Validation of UHPLC-MS/MS Method for Quantifying of Agarotriose:An Application for Pharmacokinetic,Tissue Distribution,and Excretion Studies in Rats

A sensitive,rapid,and robust ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)method was established for the first time to quantify agarotriose(A3)in rat plasma,tissues,urine,and feces.A3 and stachyose(internal standard)were separated by a BEH amide column at 65℃under the mobile phase of 10 mmol L^(-1)ammonium ace-tate-acetonitrile(42:58,v/v)with 350µLmin-1.The acquisition of transitions was carried out in multiple reaction monitoring(MRM)pattern operating with positive ionization at m/z 509.16>329.15 for A3 and m/z 689.15>527.11 for stachyose.The linearity ranges of A3 were 10 to 5000nmolL^(-1)for plasma,20 to 10000nmolL^(-1)for tissues,and 40 to 20000nmolL^(-1)for urine and feces.The accuracy and precision ranged from 90.9%to 111.6%and 0.7%to 10.1%,respectively.The stability was between 86.1%and 102.5%.The extraction recovery was consistent and reproducible.The matrix effect ranged from 1.5%to 11.4%.The pharmacokinetic,tissue dis-tribution,and excretion studies were successfully conducted with the validated method.Results showed that A3 could be absorbed by rats,and the absolute bioavailability was 6.7%.Furthermore,it was rapidly distributed in rat tissues and mainly eliminated via feces excretion(67.0%)after oral administration.For intravenous bolus,85.5%was recovered,and renal excretion was the primary path-way(77.6%)for cumulative recovery.

UHPLC-MS/MS法检测化妆品中禁用原料非那西丁

建立了灵敏检测化妆品中禁用原料非那西丁的超高效液相色谱-三重四极杆串联质谱(UHPLC-MS/MS)法。针对染发剂、烫发剂、膏霜、乳液和液态水基样品,用饱和氯化钠分散,乙腈作为提取溶剂进行超声辅助液液萃取。采用Poroshell 120 EC-C_(18)(100 mm×2.1 mm×2.7μm)色谱柱,水和乙腈分别作为流动相。串联质谱检测器采用ESI源,在多反应监测(MRM)模式下进行检测,并进行了方法评价。该方法线性关系良好,在5种基质中的检出限为0.002~0.006 mg/kg,定量限为0.006~0.02 mg/kg,加标回收率为93.7%~113.7%,相对标准偏差(RSD)为1.0%~5.6%。对60批化妆品进行检测,其中3批染发剂样品和1批烫发剂样品中检出非那西丁。该方法操作简便、灵敏度高,适用于化妆品中禁用原料非那西丁的准确检测。

UHPLC-MS/MS法同时测定三七中16个皂苷类成分在大鼠体内的含量及药代动力学研究

目的:建立UHPLC-MS/MS法同时测定三七中16个皂苷类成分(人参皂苷Rf、Rg_(1)、Rg_(2)、Re、Rd、Rb_(1)、Rb_(2)、Rb_(3)、Rc、Fa、Rk_(1)、Rg_(5)、Rg_(3)、F_(2)及三七皂苷R_(1)、Fc)在大鼠血浆中的含量,并探讨其药代动力学特征。方法:血浆样品经甲醇-乙腈(4∶1)沉淀蛋白处理后,采用ACQUITY UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)色谱柱;以0.1%甲酸溶液-乙腈为流动相,梯度洗脱;采用电喷雾离子源(ESI);以多反应监测(MRM)方式进行负离子检测,利用DAS 3.0软件计算药动学参数。结果:16个皂苷类成分在相应的浓度范围内与峰面积的线性关系良好(r>0.9900);日内、日间精密度的RSD为0.88%~14.41%,准确度为89.60%~114.00%;提取回收率为84.67%~111.87%,基质效应为77.04%~117.64%。结论:该研究建立的UHPLC-MS/MS分析方法准确、灵敏,可用于三七中16个皂苷类成分在大鼠体内的含量测定及药代动力学研究。

UHPLC-MS/MS法同时测定壮骨关节胶囊中19种成分

目的 建立UHPLC-MS/MS法同时测定壮骨关节胶囊中毛蕊花糖苷、淫羊藿苷、朝藿定A、朝藿定B、朝藿定C、柚皮苷、川续断皂苷Ⅵ、槲皮素、槲皮苷、异槲皮苷、补骨脂素、异补骨脂素、补骨脂酚、补骨脂甲素、补骨脂乙素、新补骨脂异黄酮、刺芒柄花素、二氢欧山芹醇当归酸酯、蛇床子素的含量。方法 该药物甲醇提取液的分析采用Hypersil GOLD Selectivity C18色谱柱(2.1 mm×100 mm, 1.9μm);流动相0.1%甲酸-乙腈,梯度洗脱;体积流量0.2 mL/min;柱温40℃;电喷雾离子源;正负离子扫描;多反应监测模式。结果 19种成分在各自范围内线性关系良好(r>0.990),平均加样回收率98.55%~102.01%,RSD 0.98%~2.89%。结论 该方法简便、灵敏、稳定,可用于壮骨关节胶囊的质量控制。

基于UHPLC-MS/MS技术研究蒺藜炒制前后7个甾体皂苷在大鼠体内的组织分布

目的:考察生、炒蒺藜提取物中polianthoside D、蒺藜皂苷K、tribuluside A、terrestrinin D、蒺藜呋甾皂苷B、蒺藜皂苷D和海柯皂苷元在大鼠体内的组织分布情况,探讨蒺藜的炒制原理。方法:分别灌胃给予SD大鼠生、炒蒺藜提取物,采用UHPLC-MS/MS法同时测定大鼠组织(心、肝、脾、肺、肾、脑、肠)中7个甾体皂苷的含量,研究炒制对蒺藜中7个成分在大鼠体内组织分布的影响。结果:建立的方法专属性、精密度、准确度、提取回收率、基质效应和稳定性等均符合生物样品分析要求;各组织样本在检测浓度范围内线性关系良好;蒺藜炒制前后7个甾体皂苷主要分布的组织、组织分布的最高浓度及最高浓度出现的时间点等存在一定差异。结论:炒制对蒺藜甾体皂苷类成分在大鼠体内的组织分布具有较大影响,该研究结果为阐明蒺藜炮制原理奠定了基础。

IC-MS和UHPLC-MS/MS方法测定地表水中丁基黄原酸和苦味酸

建立了直接进样离子色谱-质谱(IC-MS)和超高效液相色谱-串联质谱(UHPLC-MS/MS)测定地表水中丁基黄原酸和苦味酸的方法,2种方法简便易行,且灵敏度高。比较了2种方法测定丁基黄原酸和苦味酸的检出限、准确度和精密度,并用这2种方法同时分析了22份实际水样品中丁基黄原酸和苦味酸的质量浓度,结果均未检出。2种方法均能满足检测的要求,通过试验结果发现UHPLC-MS/MS法的检出限低于IC-MS法,但不能同时检测丁基黄原酸和苦味酸,而IC-MS法在符合方法要求的同时,可一次性检测这2种物质。因此,在要求同时检定水样中丁基黄原酸和苦味酸2种有机物时,推荐优先使用IC-MS法。

A Review on Sources,Extractions and Analysis Methods of a Sustainable Biomaterial:Tannins

Condensed and hydrolysable tannins are non-toxic natural polyphenols that are a commercial commodity industrialized for tanning hides to obtain leather and for a growing number of other industrial applications mainly to substitute petroleum-based products.They are a definite class of sustainable materials of the forestry industry.They have been in operation for hundreds of years to manufacture leather and now for a growing number of applications in a variety of other industries,such as wood adhesives,metal coating,pharmaceutical/medical applications and several others.This review presents the main sources,either already or potentially commercial of this forestry by-materials,their industrial and laboratory extraction systems,their systems of analysis with their advantages and drawbacks,be these methods so simple to even appear primitive but nonetheless of proven effectiveness,or very modern and instrumental.It constitutes a basic but essential summary of what is necessary to know of these sustainable materials.In doing so,the review highlights some of the main challenges that remain to be addressed to deliver the quality and economics of tannin supply necessary to fulfill the industrial production requirements for some materials-based uses.

Comparative permeability of three saikosaponins and corresponding saikogenins in Caco-2 model by a validated UHPLC-MS/MS method

Saikosaponins(SSs)are the main active components extracted from Bupleuri Radix(BR)which has been used as an important herbal drug in Asian countries for thousands of years.It has been reported that the intestinal bacteria plays an important role in the in vivo disposal of oral SSs.Although the deglycosylated derivatives(saikogenins,SGs)of SSs metabolized by the intestinal bacteria are speculated to be the main components absorbed into the blood after oral administration of SSs,no studies have been reported on the characteristics of SGs for their intestinal absorption,and those for SSs are also limited.Therefore,a rapid UHPLC-MS/MS method was developed to investigate and compare the apparent permeability of three common SSs(SSa,SSd,SSb2)and their corresponding SGs(SGF,SGG,SGD)through a bidirectional transport experiment on Caco-2 cell monolayer model.The method was validated according to the latest FDA guidelines and applied to quantify the six analytes in transport medium samples extracted via liquid-liquid extraction(LLE).The apparent permeability coefficient(Papp)determined in this study indicated that the permeability of SGs improved to the moderate class compared to the corresponding parent compounds,predicting a higher in vivo absorption.Moreover,the efflux ratio(ER)value demonstrated an active uptake of SSd and the three SGs,while a passive diffusion of SSa and SSb2.

Drilling-based measuring method for the c-φ parameter of rock and its field application

The technology of drilling tests makes it possible to obtain the strength parameter of rock accurately in situ. In this paper, a new rock cutting analysis model that considers the influence of the rock crushing zone(RCZ) is built. The formula for an ultimate cutting force is established based on the limit equilibrium principle. The relationship between digital drilling parameters(DDP) and the c-φ parameter(DDP-cφ formula, where c refers to the cohesion and φ refers to the internal friction angle) is derived, and the response of drilling parameters and cutting ratio to the strength parameters is analyzed. The drillingbased measuring method for the c-φ parameter of rock is constructed. The laboratory verification test is then completed, and the difference in results between the drilling test and the compression test is less than 6%. On this basis, in-situ rock drilling tests in a traffic tunnel and a coal mine roadway are carried out, and the strength parameters of the surrounding rock are effectively tested. The average difference ratio of the results is less than 11%, which verifies the effectiveness of the proposed method for obtaining the strength parameters based on digital drilling. This study provides methodological support for field testing of rock strength parameters.

A comparison study on structure-function relationship of polysaccharides obtained from sea buckthorn berries using different methods:antioxidant and bile acid-binding capacity

In this study,the structural characters,antioxidant activities and bile acid-binding ability of sea buckthorn polysaccharides(HRPs)obtained by the commonly used hot water(HRP-W),pressurized hot water(HRP-H),ultrasonic(HRP-U),acid(HRP-C)and alkali(HRP-A)assisted extraction methods were investigated.The results demonstrated that extraction methods had significant effects on extraction yield,monosaccharide composition,molecular weight,particle size,triple-helical structure,and surface morphology of HRPs except for the major linkage bands.Thermogravimetric analysis showed that HRP-U with filamentous reticular microstructure exhibited better thermal stability.The HRP-A with the lowest molecular weight and highest arabinose content possessed the best antioxidant activities.Moreover,the rheological analysis indicated that HRPs with higher galacturonic acid content and molecular weight showed higher viscosity and stronger crosslinking network(HRP-C,HRP-W and HRP-U),which exhibited stronger bile acid binding capacity.The present findings provide scientific evidence in the preparation technology of sea buckthorn polysaccharides with good antioxidant and bile acid binding capacity which are related to the structure affected by the extraction methods.

UHPLC-MS/MS法测定鸡蛋中11种头孢菌素的残留量

采用UHPLC-MS/MS技术建立了鸡蛋中11种头孢菌素含量的同步测定方法。鸡蛋样品采用60%乙腈溶液进行超声波提取,离心后以阴离子小柱(PAX)净化除杂后,再以UHPLC-MS/MS进行分析测定。在最优条件下进行方法验证,在0.5~50 ng/mL质量浓度范围具有较好的线性关系,11种头孢菌素的相关系数(R^(2))均高于0.99;鸡蛋中头孢菌素的检出限(LOD)均为0.2μg/kg,定量限(LOQ)均为0.5μg/kg;以阴性鸡蛋样品为基质分别添加1,2,10μg/kg的加标回收试验(n=6),11种头孢菌素的平均回收率在81.7%~97.1%,平行实验的RSD在2.8%~6.8%。建立的方法具有高灵敏度、高准确度和高精密度的特点,可用于鸡蛋中11种头孢菌素含量的测定。

UHPLC-MS/MS法同时测定金红片中6种活性成分的含量

目的 建立UHPLC-MS/MS法同时测定金红片中川楝素、延胡索甲素、延胡素乙素、木香烃内酯、莽草酸、去氢木香内酯6种活性成分的含量。方法 选用Infinitylab Poroshell 120 EC-C_(18)色谱柱(100 mm×4.6 mm, 2.7μm),以体积分数0.01%甲酸水溶液和体积分数0.01%甲酸乙腈溶液作为流动相进行梯度洗脱,流速为0.5 mL·min~(-1),木香烃内酯、延胡索甲素、去氢木香内酯、延胡索乙素的离子源为ESI+,川楝素和莽草酸的离子源为ESI-,采用多反应监测扫描方式检测。结果 莽草酸在质量浓度10~1 000μg·L~(-1)内呈现良好的线性关系(r=0.999 6),其余5种活性成分的质量浓度在1~100μg·L~(-1)内呈现良好的线性关系(r=0.993 5~0.999 4),平均加样回收率为91.2%~109.0%。结论 本研究可用于金红片中多种活性成分的含量测定,为金红片质量控制的完善提供参考。

Volumetric lattice Boltzmann method for pore-scale mass diffusionadvection process in geopolymer porous structures

Porous materials present significant advantages for absorbing radioactive isotopes in nuclear waste streams.To improve absorption efficiency in nuclear waste treatment,a thorough understanding of the diffusion-advection process within porous structures is essential for material design.In this study,we present advancements in the volumetric lattice Boltzmann method(VLBM)for modeling and simulating pore-scale diffusion-advection of radioactive isotopes within geopolymer porous structures.These structures are created using the phase field method(PFM)to precisely control pore architectures.In our VLBM approach,we introduce a concentration field of an isotope seamlessly coupled with the velocity field and solve it by the time evolution of its particle population function.To address the computational intensity inherent in the coupled lattice Boltzmann equations for velocity and concentration fields,we implement graphics processing unit(GPU)parallelization.Validation of the developed model involves examining the flow and diffusion fields in porous structures.Remarkably,good agreement is observed for both the velocity field from VLBM and multiphysics object-oriented simulation environment(MOOSE),and the concentration field from VLBM and the finite difference method(FDM).Furthermore,we investigate the effects of background flow,species diffusivity,and porosity on the diffusion-advection behavior by varying the background flow velocity,diffusion coefficient,and pore volume fraction,respectively.Notably,all three parameters exert an influence on the diffusion-advection process.Increased background flow and diffusivity markedly accelerate the process due to increased advection intensity and enhanced diffusion capability,respectively.Conversely,increasing the porosity has a less significant effect,causing a slight slowdown of the diffusion-advection process due to the expanded pore volume.This comprehensive parametric study provides valuable insights into the kinetics of isotope uptake in porous structures,facilitating the development of porous materials for nuclear waste treatment applications.

Material point method simulation of hydro-mechanical behaviour in twophase porous geomaterials: A state-of-the-art review

The material point method(MPM)has been gaining increasing popularity as an appropriate approach to the solution of coupled hydro-mechanical problems involving large deformation.In this paper,we survey the current state-of-the-art in the MPM simulation of hydro-mechanical behaviour in two-phase porous geomaterials.The review covers the recent advances and developments in the MPM and their extensions to capture the coupled hydro-mechanical problems involving large deformations.The focus of this review is aiming at providing a clear picture of what has or has not been developed or implemented for simulating two-phase coupled large deformation problems,which will provide some direct reference for both practitioners and researchers.

Sparse Modal Decomposition Method Addressing Underdetermined Vortex-Induced Vibration Reconstruction Problem for Marine Risers

When investigating the vortex-induced vibration(VIV)of marine risers,extrapolating the dynamic response on the entire length based on limited sensor measurements is a crucial step in both laboratory experiments and fatigue monitoring of real risers.The problem is conventionally solved using the modal decomposition method,based on the principle that the response can be approximated by a weighted sum of limited vibration modes.However,the method is not valid when the problem is underdetermined,i.e.,the number of unknown mode weights is more than the number of known measurements.This study proposed a sparse modal decomposition method based on the compressed sensing theory and the Compressive Sampling Matching Pursuit(Co Sa MP)algorithm,exploiting the sparsity of VIV in the modal space.In the validation study based on high-order VIV experiment data,the proposed method successfully reconstructed the response using only seven acceleration measurements when the conventional methods failed.A primary advantage of the proposed method is that it offers a completely data-driven approach for the underdetermined VIV reconstruction problem,which is more favorable than existing model-dependent solutions for many practical applications such as riser structural health monitoring.