Objective To investigate the variability of human cytomegalovirus （HCMV） UL138 open reading frame （ORF） in clinical strains. Methods HCMV UL138 ORF was amplified by polymerase chain reaction （PCR） and PCR amplif...Objective To investigate the variability of human cytomegalovirus （HCMV） UL138 open reading frame （ORF） in clinical strains. Methods HCMV UL138 ORF was amplified by polymerase chain reaction （PCR） and PCR amplification products were sequenced directly, and the data were analyzed in 19 clinical strains. Results LIL138 ORF in all 30 clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identifies of LIL138 ORF in all strains were 97.41% to 99.41% and 98.24% to 99.42%, respectively. All of the nucleofide mutations were substitutions. The spatial structure and post-translational modification sites of HL138 encoded proteins were conserved. The result of phylogenetic tree showed that HCMV HL138 sequence variations were not definitely related with different clinical symptoms. Conclusion HCMV UL138 ORF in clinical strains is high conservation, which might be helpful for UL138 encoded protein to play a role in latent infection of HCMV.展开更多
目的:探讨大肠癌患者人巨细胞病毒(human cytomegalovirus,HCMV)感染情况及其临床相关性.方法:采用化学发光(CLIA)法对大肠癌组(n=60)、大肠息肉组(n=60)及健康对照组(n=60)的外周血清进行HCMV免疫球蛋白G(immunoglobulin G,Ig G)、Ig ...目的:探讨大肠癌患者人巨细胞病毒(human cytomegalovirus,HCMV)感染情况及其临床相关性.方法:采用化学发光(CLIA)法对大肠癌组(n=60)、大肠息肉组(n=60)及健康对照组(n=60)的外周血清进行HCMV免疫球蛋白G(immunoglobulin G,Ig G)、Ig M抗体检测;巢式PCR技术结合原位杂交方法检测32例大肠癌组织及癌旁正常肠黏膜组织中HCMV-UL138基因表达;用Fisher确切概率法比较两组间阳性率,均数比较采用t检验或单因素方差分析.P<0.05为差异有统计学意义.结果:大肠癌组、大肠息肉组、健康对照组血清H C M V-I g G阳性率为分别为95.0%(57/60)、98.3%(59/60)、96.7%(58/60);血清HCMV-Ig M阳性率分别为5.0%(3/60)、1.7%(1/60)、1.7%(1/60),3组血清H C M VIg G、Ig M差异无统计学意义(P>0.05).32例大肠癌组织巢式PCR检测HCMV-UL138阳性率为65.6%(19/32),相应癌旁正常肠黏膜组织中为12.5%(4/32),原位杂交法检测癌组织HCMV-UL138阳性率为62.5%(20/32),癌旁正常肠黏膜组织中为9.4%(3/32),大肠癌组织HCMV阳性检出率较癌旁正常组织显著升高(P<0.01).未发现HCMV感染与大肠癌患者的年龄、性别、肿块位置、肿块大小、组织病理分化类型、转移及Dukes分期有显著性关联.结论:大肠癌组织中存在HCMV感染,且H C M V相较于癌旁正常组织更倾向感染癌灶,提示HCMV感染可能参与大肠癌的发生发展过程.展开更多
基金Supported by the National Natural Science Foundation of China (30801254)
文摘Objective To investigate the variability of human cytomegalovirus （HCMV） UL138 open reading frame （ORF） in clinical strains. Methods HCMV UL138 ORF was amplified by polymerase chain reaction （PCR） and PCR amplification products were sequenced directly, and the data were analyzed in 19 clinical strains. Results LIL138 ORF in all 30 clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identifies of LIL138 ORF in all strains were 97.41% to 99.41% and 98.24% to 99.42%, respectively. All of the nucleofide mutations were substitutions. The spatial structure and post-translational modification sites of HL138 encoded proteins were conserved. The result of phylogenetic tree showed that HCMV HL138 sequence variations were not definitely related with different clinical symptoms. Conclusion HCMV UL138 ORF in clinical strains is high conservation, which might be helpful for UL138 encoded protein to play a role in latent infection of HCMV.
文摘目的:探讨大肠癌患者人巨细胞病毒(human cytomegalovirus,HCMV)感染情况及其临床相关性.方法:采用化学发光(CLIA)法对大肠癌组(n=60)、大肠息肉组(n=60)及健康对照组(n=60)的外周血清进行HCMV免疫球蛋白G(immunoglobulin G,Ig G)、Ig M抗体检测;巢式PCR技术结合原位杂交方法检测32例大肠癌组织及癌旁正常肠黏膜组织中HCMV-UL138基因表达;用Fisher确切概率法比较两组间阳性率,均数比较采用t检验或单因素方差分析.P<0.05为差异有统计学意义.结果:大肠癌组、大肠息肉组、健康对照组血清H C M V-I g G阳性率为分别为95.0%(57/60)、98.3%(59/60)、96.7%(58/60);血清HCMV-Ig M阳性率分别为5.0%(3/60)、1.7%(1/60)、1.7%(1/60),3组血清H C M VIg G、Ig M差异无统计学意义(P>0.05).32例大肠癌组织巢式PCR检测HCMV-UL138阳性率为65.6%(19/32),相应癌旁正常肠黏膜组织中为12.5%(4/32),原位杂交法检测癌组织HCMV-UL138阳性率为62.5%(20/32),癌旁正常肠黏膜组织中为9.4%(3/32),大肠癌组织HCMV阳性检出率较癌旁正常组织显著升高(P<0.01).未发现HCMV感染与大肠癌患者的年龄、性别、肿块位置、肿块大小、组织病理分化类型、转移及Dukes分期有显著性关联.结论:大肠癌组织中存在HCMV感染,且H C M V相较于癌旁正常组织更倾向感染癌灶,提示HCMV感染可能参与大肠癌的发生发展过程.
