ISSR Analysis on Genetic Diversity of Local Varieties of Chinese Hu mulberry(Morus L.)

[Objective] The aim was to study on the genetic diversity of local varieties of Chinese Hu mulberry (Morus L.). [Method] The genetic diversity of 141 copies of Hu mulberry varieties was analyzed by ISSR molecular markers. [Result] 12 ISSR primers had amplified a total of 90 amplified,of which 57 bands were polymorphic,and the polymorphic rate was 63.33%. The genetic similarity coefficients of 141 Hu mulberry germplasm resources varied from 0.633 3 to 1.000 0 with the average of 0.483 35,indicating that there was difference on genetic diversity among different varieties of Hu mulberries. A dendrogram of all 141 Hu mulberry varieties based on the genetic similarity coefficients using ISSR molecular markers was generated by UPGMA cluster method. Clustering of the 141 Hu mulberry varieties did not correspond with the conventional classification involving differences in style,leaf,branch,fruit and other morphological or agronomical characters. [Conclusion] Four subgroups clearly represented the genetic relationships in the 141 accessions which were benefit for the variety improvement and germplasm resource conservation.

新型联合片段:nrDNA ITS+nad 1-intron 2在枫斗类石斛鉴定中的意义

本文从线粒体基因片段入手,利用PCR技术扩增出17种枫斗类石斛39个个体的线粒体基因co I、nad 5部分序列及nad 1-intron 2序列,nr DNA ITS及叶绿体基因序列rbc L、mat K、psb A-trn H,同时筛选出合适的基因片段来研究多片段联合在枫斗类石斛植物鉴定方面的应用。结果表明:在7个基因片段中,nr DNA ITS、nad 1-intron 2和psb A-trn H的序列变异度较高,可用于枫斗类石斛的鉴定。但是,仅利用单一序列无法对细茎石斛及霍山石斛进行鉴别。而nr DNA ITS+nad 1-intron 2新型联合片段构建的UPGMA树与其他联合片段相比,更能有效的对枫斗类石斛基原植物进行鉴别并可区分细茎石斛及霍山石斛。此外,借助nr DNA ITS+nad 1-intron 2新型联合片段构建的UPGMA树对3个待检种进行了成功鉴别,分别为霍山石斛、细茎石斛和铁皮石斛。本研究首次将nr DNA ITS与线粒体基因片段联合用于枫斗类石斛的鉴定,为石斛药材的鉴别提供了可靠的科学依据,同时也将对规范枫斗类石斛市场提供理论指导。