The objective of the present research work is to develop a gradient, reversed-phase liquid chromatographic (RP-UPLC) method for the determination of Finasteride in pharmaceutical bulk drugs for assay and its related i...The objective of the present research work is to develop a gradient, reversed-phase liquid chromatographic (RP-UPLC) method for the determination of Finasteride in pharmaceutical bulk drugs for assay and its related impurities. The chromatographic separation was achieved on a Waters ACQUITY UPLC BEH Phenyl Column (150 mm × 2.1 mm, 1.7 μm), The gradient LC method employs solutions A and B as mobile phase. The solution A Contains 2.5 mM ortho phosphoric acid (Buffer) and solution B contains a mixture of acetonitrile and water in the ratio of (90:10 v/v). The flow rate was 0.22 ml/min and the detection wavelength was 210 nm. In the developed UPLC method, the resolution between Finasteride and its potential impurities, namely Imp-1, Imp-2, Imp-3 and Imp-4 was found to be greater than 2.0. The drug was subjected to stress conditions of hydrolysis, oxidation, photolysis and thermal degradation. Considerable degradation was found to occur in alkaline medium and oxidative stress conditions. Degradation product formed during oxidative hydrolysis was found to be Imp-1. The stress samples were assayed against a qualified reference standard and the mass balance was found close to 99.5%. The developed RP-UPLC method was validated with respect to linearity, accuracy, precision and robustness. The limit of quantification of Imp-1, Imp-2, Imp-3 and Imp-4 were 0.06, 0.06, 0.05 and 0.036% (of analyte concentration, i.e. 0.5 mg/ml) with 1μl injection volume. The developed method was found to be linear in the range of 2.5 - 15 μg/mL with correlation coefficient of 0.999 for assay procedures and found to be linear in the range of 0.05 - 3 μg/mL with correlation coefficient of 0.999 for related impurities.展开更多
目的:采用UPLC-Q-TOF-MS法测定扶正膏药渣中特女贞苷、丹皮酚、芒柄花素、藁本内酯的含量。方法:采用安捷伦ZORBAX Eclipse Plus C 18柱(3.0 mm×100 mm,1.8μm),以乙腈-0.05 mmol·L^(-1)甲酸铵溶液为流动相进行梯度洗脱,流速...目的:采用UPLC-Q-TOF-MS法测定扶正膏药渣中特女贞苷、丹皮酚、芒柄花素、藁本内酯的含量。方法:采用安捷伦ZORBAX Eclipse Plus C 18柱(3.0 mm×100 mm,1.8μm),以乙腈-0.05 mmol·L^(-1)甲酸铵溶液为流动相进行梯度洗脱,流速为0.4 mL/min,进样量为2.0μL,柱温为30℃;质谱条件如下:离子源为ESI,正、负模式下采集数据,喷雾电压4000 V;离子化温度325℃,外标法定量。结果:特女贞苷、丹皮酚、芒柄花素、藁本内酯的线性范围分别为1.0~100.0,1.0~100.0,1.1~112.0,0.5~50.0μg/L,且线性关系均良好(R≥0.9990);精密度、稳定性、重复性试验结果显示4个中药成分的峰面积RSD均≤5.0%;4个中药成分的平均加样回收率分别为94.90%~109.14%(RSD<4.0%,n=6)。结论:建立的UPLC-Q-TOF-MS法检测扶正膏药渣中的特女贞苷、丹皮酚、芒柄花素、藁本内酯的含量结果准确可靠,证实了药渣中还残留一定量的活性成分,具有一定的营养价值,为药渣的综合开发利用提供依据。展开更多
文摘The objective of the present research work is to develop a gradient, reversed-phase liquid chromatographic (RP-UPLC) method for the determination of Finasteride in pharmaceutical bulk drugs for assay and its related impurities. The chromatographic separation was achieved on a Waters ACQUITY UPLC BEH Phenyl Column (150 mm × 2.1 mm, 1.7 μm), The gradient LC method employs solutions A and B as mobile phase. The solution A Contains 2.5 mM ortho phosphoric acid (Buffer) and solution B contains a mixture of acetonitrile and water in the ratio of (90:10 v/v). The flow rate was 0.22 ml/min and the detection wavelength was 210 nm. In the developed UPLC method, the resolution between Finasteride and its potential impurities, namely Imp-1, Imp-2, Imp-3 and Imp-4 was found to be greater than 2.0. The drug was subjected to stress conditions of hydrolysis, oxidation, photolysis and thermal degradation. Considerable degradation was found to occur in alkaline medium and oxidative stress conditions. Degradation product formed during oxidative hydrolysis was found to be Imp-1. The stress samples were assayed against a qualified reference standard and the mass balance was found close to 99.5%. The developed RP-UPLC method was validated with respect to linearity, accuracy, precision and robustness. The limit of quantification of Imp-1, Imp-2, Imp-3 and Imp-4 were 0.06, 0.06, 0.05 and 0.036% (of analyte concentration, i.e. 0.5 mg/ml) with 1μl injection volume. The developed method was found to be linear in the range of 2.5 - 15 μg/mL with correlation coefficient of 0.999 for assay procedures and found to be linear in the range of 0.05 - 3 μg/mL with correlation coefficient of 0.999 for related impurities.
文摘目的:采用UPLC-Q-TOF-MS法测定扶正膏药渣中特女贞苷、丹皮酚、芒柄花素、藁本内酯的含量。方法:采用安捷伦ZORBAX Eclipse Plus C 18柱(3.0 mm×100 mm,1.8μm),以乙腈-0.05 mmol·L^(-1)甲酸铵溶液为流动相进行梯度洗脱,流速为0.4 mL/min,进样量为2.0μL,柱温为30℃;质谱条件如下:离子源为ESI,正、负模式下采集数据,喷雾电压4000 V;离子化温度325℃,外标法定量。结果:特女贞苷、丹皮酚、芒柄花素、藁本内酯的线性范围分别为1.0~100.0,1.0~100.0,1.1~112.0,0.5~50.0μg/L,且线性关系均良好(R≥0.9990);精密度、稳定性、重复性试验结果显示4个中药成分的峰面积RSD均≤5.0%;4个中药成分的平均加样回收率分别为94.90%~109.14%(RSD<4.0%,n=6)。结论:建立的UPLC-Q-TOF-MS法检测扶正膏药渣中的特女贞苷、丹皮酚、芒柄花素、藁本内酯的含量结果准确可靠,证实了药渣中还残留一定量的活性成分,具有一定的营养价值,为药渣的综合开发利用提供依据。