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Progress and Challenges of Reference Standard and Its New Form: Digital Reference Standard 被引量:8
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作者 Qingjun Wang Lei Sun +4 位作者 Feng Liu Hongyu Jin Jiandong Yu Zhong Dai Shuangcheng Ma 《Chinese Medicine》 2016年第2期77-91,共15页
Number and quantities of reference standards (RS) needed for the quality control of drugs are increasing, bringing great pressure to the calibration and using company. This manuscript summarized the four generations f... Number and quantities of reference standards (RS) needed for the quality control of drugs are increasing, bringing great pressure to the calibration and using company. This manuscript summarized the four generations for the development of RS including physical RS, paper atlas, substitute RS and electronic databases. The advantages and disadvantages of each generation were summarized. The concept of digital RS (DRS) was proposed based on this, and summed up the definition, advantages, and technical architecture of DRS. The 10 characteristics of five aspects of the DRS were discussed including digital, multi-dimension, big data, cloud computing, internet, internet of things, sharing, multi-terminal, intelligence, and compliance certification. Then, the necessity of its formation and application in the medicine holistic quality control of internet plus era was discussed in this manuscript. 展开更多
关键词 digital reference Standards Herbal reference Standards Extractive reference Standards Substitute reference Standards Holistic Quality Control Internet Plus Big Data DATABASE
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Equalization Reconstruction Algorithm Based on Reference Signal Frequency Domain Block Joint for DTMB-Based Passive Radar
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作者 Shuai Ma Zeqi Yang +2 位作者 Hua Zhang Yiheng Liu Xiaode Lyu 《Journal of Beijing Institute of Technology》 EI CAS 2024年第1期41-53,共13页
Channel equalization plays a pivotal role within the reconstruction phase of passive radar reference signals.In the context of reconstructing digital terrestrial multimedia broadcasting(DTMB)signals for low-slow-small... Channel equalization plays a pivotal role within the reconstruction phase of passive radar reference signals.In the context of reconstructing digital terrestrial multimedia broadcasting(DTMB)signals for low-slow-small(LSS)target detection,a novel frequency domain block joint equalization algorithm is presented in this article.From the DTMB signal frame structure and channel multipath transmission characteristics,this article adopts a unconventional approach where the delay and frame structure of each DTMB signal frame are reconfigured to create a circular convolution block,facilitating concurrent fast Fourier transform(FFT)calculations.Following equalization,an inverse fast Fourier transform(IFFT)-based joint output and subsequent data reordering are executed to finalize the equalization process for the DTMB signal.Simulation and measured data confirm that this algorithm outperforms conventional techniques by reducing signal errors rate and enhancing real-time processing.In passive radar LSS detection,it effectively suppresses multipath and noise through frequency domain equalization,reducing false alarms and improving the capabilities of weak target detection. 展开更多
关键词 passive radar frequency domain equalization reference signal reconstruction digital terrestrial multimedia broadcasting(DTMB)
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Applicahon of a Simplified Model Reference Adaptive Algorithm 被引量:1
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作者 王丽艳 宫小东 +1 位作者 金志立 曹泛 《Journal of Beijing Institute of Technology》 EI CAS 1994年第1期90+82-90,共10页
The application of a simplifed model reference adaptive control(SMRAC) on a typical Pump controlled motor electrohydraulic servo system is studied here. The algorithm of first-order scalar SMRAC ac second-order vector... The application of a simplifed model reference adaptive control(SMRAC) on a typical Pump controlled motor electrohydraulic servo system is studied here. The algorithm of first-order scalar SMRAC ac second-order vector SMRAC are derived. Computer simulations of the algorithms are presented. Experimental results prove that the method of control adopted here perform satisfactorily over a wide range of operating conditions. 展开更多
关键词 model reference adaptive control digital control electrohydraulic servo system
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Special Issue on Future Digital Design and Manufacturing:Embracing Industry 4.0 and Beyond 被引量:6
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作者 QIN Shengfeng CHENG Kai 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2016年第6期1045-1045,共1页
Industry 4.0 as referred to the fourth industrial revolution has endorsed in several national manufacturing initiatives or development plans such as in Germany, the UK, USA and China. A set of important pervasive and ... Industry 4.0 as referred to the fourth industrial revolution has endorsed in several national manufacturing initiatives or development plans such as in Germany, the UK, USA and China. A set of important pervasive and secondary technologies for future manufacturing activities have been identified such as additive manufacturing, sensor technology, 展开更多
关键词 DESIGN In usa Special Issue on Future digital Design and Manufacturing
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Synchronization of Digital Chaos in Secure Communication Systems 被引量:3
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作者 Geng Zhao, Deling Zheng, ShaojunHuang 1)College of Electric Science and Technology Beijing. Beijing 100070, China 2)Information Engineering School, University of Science and Technology Beijing. Beijing 100083, China 《Journal of University of Science and Technology Beijing》 CSCD 2001年第4期299-305,共7页
The theories of synchronization based on secure communications using digital chaos are presented. A new synchronous method-cycles-interval Pulse drive is developed and realized. Experimental results show it is availab... The theories of synchronization based on secure communications using digital chaos are presented. A new synchronous method-cycles-interval Pulse drive is developed and realized. Experimental results show it is available, and in order to reduce synchronous noise, a method using model references solves the ratio of signal power to noise power, so the secure communication system can be realized. 展开更多
关键词 digital chaotic synchronization cycles-interval pulses drive synchronous noise model reference
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makeTwin:A reference architecture for digital twin software platform 被引量:4
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作者 Fei TAO Xuemin SUN +12 位作者 Jiangfeng CHENG Yonghuai ZHU Weiran LIU Yong WANG Hui XU Tianliang HU Xiaojun LIU Tingyu LIU Zheng SUN Jun XU Jinsong BAO Feng XIANG Xiaohui JIN 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2024年第1期1-18,共18页
Digital twin is currently undergoing a significant transformation from the conceptual and theoretical research phase to the implementation and application phase.However,a universally adaptable research and development... Digital twin is currently undergoing a significant transformation from the conceptual and theoretical research phase to the implementation and application phase.However,a universally adaptable research and development platform for digital twin is critically needed to meet the development requirements.Specifically,a publicly accessible simulation,testing,and validation platform which can support digital twin model building,data processing,algorithm design,configuration,etc.,is urgently required for researchers.Furthermore,for developers from the industry,a lowcode development platform that can offer a customizable suite of functions such as model creation,data management,protocol configuration,and visualization is urgently needed.Meanwhile,for enterprise users,there is a lack of an application management platform that can be configured and migrated for various application scenarios,functions,and modes.Therefore,based on the system research of digital twin theories and key technologies by the authors(such as the five-dimension digital twin model,digital twin modeling and digital twin data theory,digital twin standards,and so on),a digital twin software platform reference architecture,namely make Twin,is proposed and designed,as well as its ten core functions.The workflow of the make Twin and the interaction mechanisms among its core functions are described.Finally,a digital twin application system for a chemical fiber textile shop floor(CFTS)which was developed according to make Twin,is introduced,which validates the proposed reference architecture. 展开更多
关键词 digital twin digital twin software platform Industrial software makeTwin reference architecture
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Research on the Digital Communication Strategy of Intangible Cultural Heritage under the New Media Environment 被引量:1
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作者 Chunhui ZHENG Longtao GAI 《International Journal of Technology Management》 2013年第9期134-137,共4页
In the field of intangible cultural heritage' s digital protection, a lot of research work has been made by all countries in the world, while the research on the digital commuuication of intangible cultural heritage ... In the field of intangible cultural heritage' s digital protection, a lot of research work has been made by all countries in the world, while the research on the digital commuuication of intangible cultural heritage is few and especially rarely seen in China. Under the premise, in this paper, the factors influencing intangible cultural heritage in the communication process are analyzed based on the information characteristics of intangible cultural heritage and the advantages of I-Space framework model in researching information transfer. Also, combined with the birch bark making technology in Heilongjiang province, the knowledge-based and signified communication strategy of intangible cultural heritage is proposed, aiming at providing new ideas for the researches on the digital communication of intangible cultural heritage. 展开更多
关键词 Intangible Cultural Heritage digital communication I-Space Conceptual reference Model
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Real-Time Discrete Adaptive Control of Robot Arm Based on Digital Signal Processing
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作者 龙绪明 《Journal of Southwest Jiaotong University(English Edition)》 2008年第1期24-29,共6页
A discrete model reference adaptive controller of robot arm is obtained by integrating the reduced dynamic model of robot, model reference adaptive control (MRAC) and digital signal processing (DSP) computer syste... A discrete model reference adaptive controller of robot arm is obtained by integrating the reduced dynamic model of robot, model reference adaptive control (MRAC) and digital signal processing (DSP) computer system into an electromechanical system. With the DSP computer system, the control signal of each joint of the robot arm can be processed in real time and independently. The simulation and experiment results show that with the control strategy, the robot achieved a good trajectory following precision, a good decoupling performance and a high real-time adaptivity. 展开更多
关键词 ROBOT Model reference adaptive control (MRAC) digital signal processing (DSP) Real-time control
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Forensics: Collection of Sound Digital Evidence
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作者 Richard Kabanda Harihar Thapa Luis Rivera 《Journal of Information Security》 2023年第4期454-463,共10页
This summary paper will discuss the concept of forensic evidence and evidence collection methods. Emphasis will be placed on the techniques used to collect forensically sound digital evidence for the purpose of introd... This summary paper will discuss the concept of forensic evidence and evidence collection methods. Emphasis will be placed on the techniques used to collect forensically sound digital evidence for the purpose of introduction to digital forensics. This discussion will thereafter result in identifying and categorizing the different types of digital forensics evidence and a clear procedure for how to collect forensically sound digital evidence. This paper will further discuss the creation of awareness and promote the idea that competent practice of computer forensics collection is important for admissibility in court. 展开更多
关键词 Electronic Discovery reference Model National Institute of Standards and Technology (NIST) Message-Digest Algorithm Secure Hash Algorithm Forensically Sound digital Evidence
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Pro-Environmental Civic Participation in the USA: The Effects of Social Media, Pro-Environmental Lifestyle and Climate Experiences
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作者 Rita Mano 《American Journal of Climate Change》 2024年第1期31-46,共16页
This study addresses the link between social media use and pro-environmental civic participation considering the moderating effect of social media affordances (public realm) on one hand, and lifestyle behaviors and cl... This study addresses the link between social media use and pro-environmental civic participation considering the moderating effect of social media affordances (public realm) on one hand, and lifestyle behaviors and climate change experiences (personal realm) on the other. We combine communication theory and behavioral models and using a sample of USA individuals (N = 7225) based on the American Trends Panel to predict variations in pro-environmental behavior. We show that social networks rather than information are more effective in predicting pro-environmental behavior. Moreover, a pro-environmental lifestyle as well as climate change experiences at the community level increase the likelihood for pro-environmental participation. However, affordances related to socioeconomic variations generate variations to pro-environmental civic participation. We conclude that in order to capture the depth of pro-environmental civic participation, it is necessary to theoretically and empirically bridge between private and public expressions of pro-environmental awareness. 展开更多
关键词 Pro-Environmental Behavior SOCIO-ECONOMIC digital Information digital Networks Private Expressions Public Expressions Climate Changes usa
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国际数字化转型框架研究:比较与镜鉴 被引量:3
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作者 钟志贤 杨佳 +1 位作者 张义 蒋云良 《中国电化教育》 CSSCI 北大核心 2024年第2期79-88,共10页
数字化转型框架是转型实践中的理论模型和方法工具,有利于引导组织机构明确战略愿景、核心使命,以及实现转型目标的核心场景、动力机制和关键能力,最终帮助组织机构制定转型行动计划。以国际上75个有关企业(37个)、政府(15个)和教育(23... 数字化转型框架是转型实践中的理论模型和方法工具,有利于引导组织机构明确战略愿景、核心使命,以及实现转型目标的核心场景、动力机制和关键能力,最终帮助组织机构制定转型行动计划。以国际上75个有关企业(37个)、政府(15个)和教育(23个)三类数字化转型框架为研究样本,采用NVivo12质性分析软件分别对三类数字化转型框架进行编码。在词频和聚类编码分析、比较的基础上,探讨了三类数字化转型框架的共性和个性特征。研究发现,三类数字化转型框架的共同关键行动域是“数字战略和规划、数字技术和数据、数字人才和文化、数字信息安全与伦理、数字生态系统”;在个性特征方面,企业数字化转型框架关注客户需求和创新商业模式;政府数字化转型框架注重利用数据革新公共服务模式及其流程;教育数字化转型框架聚焦在培养师生数字素养方面。镜鉴国际经验,基于数字文明时代、转型内涵、框架特征、行动要素、实践逻辑五大视域构建了教育数字化转型框架,框架应用的成效和转型智慧的涌现,必须彰显“使用者设计”,遵循“自发秩序原理”和教育数字化转型的底层逻辑。 展开更多
关键词 教育数字化转型 国际镜鉴 比较 框架
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甲型流感病毒H1N1型灭活病毒标准物质研究
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作者 许丽 乐丽欢 +6 位作者 杨雪 王炤坤 李兰英 闻艳丽 陶晴 胡轶红 刘刚 《计量学报》 CSCD 北大核心 2024年第2期294-299,共6页
以人工培养真实病毒为原料,研制了一种甲型流感病毒H1N1型灭活病毒标准物质。该标准物质经化学灭活及灭活效果验证后,联合多家实验室采用数字PCR定量检测方法对拷贝数浓度进行定值,对标准物质的均匀性、稳定性及不确定度进行了评估。结... 以人工培养真实病毒为原料,研制了一种甲型流感病毒H1N1型灭活病毒标准物质。该标准物质经化学灭活及灭活效果验证后,联合多家实验室采用数字PCR定量检测方法对拷贝数浓度进行定值,对标准物质的均匀性、稳定性及不确定度进行了评估。结果显示该标准物质均匀性良好,在-80~-70℃保存条件下稳定保存5个月,标准物质最终定值结果为(1384.5±249.3)copies/μL(k=2)。该标准物质可为H1N1型甲型流感病毒检测的全过程提供计量溯源和质量控制支撑,有助于提升相关核酸检测结果的准确性和可靠性。 展开更多
关键词 生物计量学 甲型流感病毒 H1N1 灭活病毒 标准物质 数字PCR
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基于D-I-M协同模型的企业数字化转型财务管理风险识别研究 被引量:1
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作者 魏延辉 曹文聪 陈娅茹 《甘肃科学学报》 2024年第2期131-137,146,共8页
数字化正在重塑经济发展的轨道,成为赋能经济增长新动力。精准识别财务管理风险中的关键指标及其相互关系,对企业更好地完成数字化转型尤为关键。融合企业人力、运营、财务、环境4个基本维度,从链式传导视角构建转型背景下企业财务管理... 数字化正在重塑经济发展的轨道,成为赋能经济增长新动力。精准识别财务管理风险中的关键指标及其相互关系,对企业更好地完成数字化转型尤为关键。融合企业人力、运营、财务、环境4个基本维度,从链式传导视角构建转型背景下企业财务管理风险指标体系。通过决策试验与评价实验室(DEMATL)法,分析各影响因素之间中心度和原因度关系,并利用解释结构模型(ISM),将矩阵进一步分析得到影响因素的层级划分,归纳影响因素之间的内在指向关系,最后运用交叉影响矩阵相乘(MICMAC)法计算各因素的驱动—依赖关系以判断其属性类别。提出通过D-I-M协同分析的方法,识别财务管理风险路径,为企业进行多链融合数字化转型、规避财务管理风险提供参考。 展开更多
关键词 决策试验与评价实验室法 解释结构模型 交叉影响矩阵相乘法 数字化转型 财务风险
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病原宏基因组高通量测序生物信息学分析质量评价的研究现状与思考
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作者 刘东来 关文达 +3 位作者 吴林寰 王浩 杨子峰 许四宏 《中国食品药品监管》 2024年第5期34-41,共8页
病原宏基因组高通量测序(mNGS)技术已成为感染病原学诊断的新工具,由实验操作(湿实验)和生物信息学分析(干实验)两部分组成。干实验由算法和数据库构成,其功能是对湿实验产生的测序数据进行分析处理后输出检测结果。干实验的性能受到测... 病原宏基因组高通量测序(mNGS)技术已成为感染病原学诊断的新工具,由实验操作(湿实验)和生物信息学分析(干实验)两部分组成。干实验由算法和数据库构成,其功能是对湿实验产生的测序数据进行分析处理后输出检测结果。干实验的性能受到测序数据中复杂多变的干扰因素的影响,包括临床样本中大量的人源核酸、试剂与耗材携带的微生物核酸、采样与湿实验引入的环境微生物核酸、数据库中基因组质量不均一或不同物种基因组之间的相似性过高导致的错误比对与注释,以及算法与参数差异对分类鉴定的影响等。上述干扰因素可能来自mNGS检测各个环节,不仅可能导致干实验输出错误的物种鉴定和微生物检测结果,也给干实验的质量控制与评价带来较大挑战。本文综述了mNGS干实验质量控制的关键问题以及关于质量评价方法的思考。 展开更多
关键词 病原宏基因组高通量测序 生物信息学分析 质量评价 数字参考品
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大语言模型对数字参考咨询服务的影响与应对策略研究
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作者 吴育良 《山西档案》 北大核心 2024年第5期134-137,共4页
随着以ChatGPT为代表的大语言模型的逐渐兴起,其强大的信息整合与生成能力为数字参考咨询服务领域带来颠覆性影响。通过梳理当前研究与发展现状,从技术赋能、资源拓展、服务重塑、治理挑战、用户牵引等5个维度深入剖析了大语言模型对数... 随着以ChatGPT为代表的大语言模型的逐渐兴起,其强大的信息整合与生成能力为数字参考咨询服务领域带来颠覆性影响。通过梳理当前研究与发展现状,从技术赋能、资源拓展、服务重塑、治理挑战、用户牵引等5个维度深入剖析了大语言模型对数字参考咨询服务的影响,并从学科布局优化、服务聚焦用户、业务流程再造、平台生态构建等层面提出了变革性的应对策略,旨在系统探析大语言模型对传统数字参考咨询服务带来的挑战与机遇。这不仅丰富了数字参考咨询服务的理论认知,还为新技术环境下服务优化提供了实践指引,以期能够推动信息资源管理学科适应技术发展浪潮,实现创新发展。 展开更多
关键词 大语言模型 数字参考咨询服务 ChatGPT 学科发展 服务创新
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牛结节性皮肤病病毒(NMG株)基因组DNA标准物质的研制 被引量:5
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作者 苏佳 白洪旭 +5 位作者 赵炜 王德丽 刘伟洁 王芳 薛青红 陈晓春 《中国预防兽医学报》 CAS CSCD 北大核心 2024年第3期320-325,共6页
为提高牛结节性皮肤病病毒(LSDV)核酸检测结果的准确性,本研究通过病毒核酸提取及质量检测、分装,制备LSDV NMG株基因组DNA标准物质。通过荧光定量PCR(qPCR)检测外源病毒评价其纯净性;采用液滴式数字PCR(ddPCR)方法检测LSDV ORF101基因... 为提高牛结节性皮肤病病毒(LSDV)核酸检测结果的准确性,本研究通过病毒核酸提取及质量检测、分装,制备LSDV NMG株基因组DNA标准物质。通过荧光定量PCR(qPCR)检测外源病毒评价其纯净性;采用液滴式数字PCR(ddPCR)方法检测LSDV ORF101基因的拷贝数以评估该标准物质的均匀性、稳定性并与9家实验室协作标定;进一步采用qPCR方法对该标准物质试用。结果显示,本研究制备的标准物质不存在外源病毒污染;组内和组间无显著性差异,均匀性好;标准物质于-20℃稳定保存6个月,4℃稳定保存7d,25℃稳定保存24 h;标准物质ORF101基因最终定值为(8.6±1.3)×10~3拷贝/μL;可以用于qPCR检测。综上,该标准物质定值准确,均匀性好,不仅可以用于LSDV核酸检测相关实验的质量控制、仪器校准、检测方法评价,还可为研制体外诊断试剂提供物质基础。 展开更多
关键词 牛结节性皮肤病病毒 液滴式数字PCR 标准物质 定值
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数字PCR和荧光定量PCR检测转基因番木瓜中外源基因拷贝数方法的建立及其应用 被引量:1
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作者 谢秀菊 夏启玉 +7 位作者 刘帅 麦贤俊 贾瑞宗 郭安平 徐志胜 李峰 孔祥义 赵辉 《热带作物学报》 CSCD 北大核心 2024年第4期663-673,共11页
传统的检测转基因植物中外源基因拷贝数的方法是Southern杂交,该方法成本高、周期长,难以满足高通量检测外源基因拷贝数的育种需求,因此,本研究旨在建立快速且高通量检测转基因番木瓜中外源基因拷贝数的方法。本研究从番木瓜基因组中筛... 传统的检测转基因植物中外源基因拷贝数的方法是Southern杂交,该方法成本高、周期长,难以满足高通量检测外源基因拷贝数的育种需求,因此,本研究旨在建立快速且高通量检测转基因番木瓜中外源基因拷贝数的方法。本研究从番木瓜基因组中筛选出2个单拷贝基因Cpa03g018830和Cpa03g018770,以已知外源基因为单拷贝整合的转基因番木瓜为参照,利用数字PCR鉴定其拷贝数,进一步以其为内参基因,以番木瓜转基因育种常用的筛选标记基因NPTⅡ为外源目的基因,建立利用数字PCR和荧光定量PCR技术检测转基因番木瓜中外源基因拷贝数的方法。结果表明:Cpa03g018830和Cpa03g018770均为单拷贝基因;建立的数字PCR方法对转基因番木瓜中的外源基因拷贝数的检测准确可靠,而荧光定量PCR对转基因番木瓜中外源基因单低拷贝整合的检测准确度较高,对外源基因多拷贝整合的检测准确度则较低,因此,荧光定量PCR适合用来在大量转基因植株中初筛出单低拷贝整合的植株。本研究鉴定的单拷贝基因Cpa03g018830和Cpa03g018770可作为转基因番木瓜中外源基因拷贝数检测的内参基因,且建立的利用数字PCR和荧光定量PCR技术检测转基因番木瓜中外源基因拷贝数的方法,操作简单,速度快,适合批量检测,可为番木瓜转基因抗病育种中单低拷贝株系的选育提供新的方法。 展开更多
关键词 转基因番木瓜 拷贝数 内参基因 数字PCR 荧光定量PCR
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羊源性基因组DNA标准物质研制
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作者 纪艺 王凯莉 +5 位作者 余卉茹 赵新 丁霖 彭城 徐俊锋 陈笑芸 《生物技术进展》 2024年第1期125-132,共8页
近年来,羊肉及其肉制品的掺假等食品安全事件层出不穷,为了完善市场执法检查法律依据,利用数字PCR定值羊HELZ基因,研制了羊基因组DNA标准物质。由于标准物质可以用于衡量检测方法的准确性,因此可以判定食品及相关制品中羊肉的掺假情况... 近年来,羊肉及其肉制品的掺假等食品安全事件层出不穷,为了完善市场执法检查法律依据,利用数字PCR定值羊HELZ基因,研制了羊基因组DNA标准物质。由于标准物质可以用于衡量检测方法的准确性,因此可以判定食品及相关制品中羊肉的掺假情况。利用数字PCR对研制的标准物质进行均匀性和稳定性评估,结果表明该批标准物质均匀性良好,在4℃、25℃可以稳定保存14 d,在-20℃可以稳定保存6个月。来自全国9个不同实验室羊源性基因组DNA标准物质(高浓度)和羊源性基因组DNA标准物质(低浓度)的联合定值结果显示,标准值及其扩展不确定度分别为(5.44±0.45)×10^(3)copies·μL^(-1)和(5.68±0.54)×10^(2)copies·μL^(-1)。该羊源性基因组DNA标准物质为动物源性标准物质的市场应用提供了技术基础,完善了羊源性基因组DNA标准物质的制备、定量检测、质量控制和量值溯源技术平台。 展开更多
关键词 标准物质 羊源性HELZ基因 数字PCR
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产气荚膜梭菌α-毒素基因数字PCR方法的建立及其在标准物质研制方面的应用
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作者 张铭洋 张喜悦 +7 位作者 赵格 曲志娜 高宏伟 孙敏 程慧敏 徐佳微 王君玮 邹明 《山东农业科学》 北大核心 2024年第1期156-163,共8页
产气荚膜梭菌(Clostridium perfringens)是一种重要的人兽共患病原菌,α-毒素是其分子生物学检测的主要靶标。试验选取Clper引物及探针建立微滴式数字PCR(droplet digtial PCR,ddPCR)方法,最佳引物浓度0.85μmol/L,探针浓度为0.3μmol/L... 产气荚膜梭菌(Clostridium perfringens)是一种重要的人兽共患病原菌,α-毒素是其分子生物学检测的主要靶标。试验选取Clper引物及探针建立微滴式数字PCR(droplet digtial PCR,ddPCR)方法,最佳引物浓度0.85μmol/L,探针浓度为0.3μmol/L,退火温度为60℃。利用建立的ddPCR方法检测单核细胞增生李斯特菌、沙门氏菌、大肠杆菌、肠球菌、金黄色葡萄球菌、弯曲杆菌的DNA,结果均为阴性,表明该方法具有较好的特异性。合成产气荚膜梭菌α-毒素基因,制成DNA标准品,选取3个稀释梯度的标准品进行重复试验,组内和组间变异系数均小于5%,证明该方法具有较好的重复性。采用7个稀释梯度的标准品进行重复检测,计算出该方法的检测限为12.39 copies/μL,定量限为33.97 copies/μL。通过对68份临床样品进行检测,证实该方法可用于临床,且用于检测质粒DNA时无明显的基质效应。应用ddPCR方法对标准品进行均匀性和稳定性检验,并联合9家实验室进行定值,最终获得了国家市场监督管理总局颁发的标准物质证书(GBW(E)091237)。α-毒素基因ddPCR方法的建立和DNA标准物的研制,为产气荚膜梭菌分子生物学检测试剂的标准化奠定了基础。 展开更多
关键词 产气荚膜梭菌 ddPCR α-毒素基因 标准物质 特异性 灵敏度 可重复性 定值
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转基因定量检测量值转换系数的研究进展
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作者 刘圆圆 李佳丽 +3 位作者 吉帅帅 高运华 刘旦梅 王迪 《计量学报》 CSCD 北大核心 2024年第6期922-928,共7页
基于PCR技术测定的转基因成分定量结果主要是拷贝数比值,而“产品标识”规定检测结果必须以质量分数表示。量值单位的不统一,造成无法根据检测结果直接进行标识与否的判定。因此亟须研究转基因含量2种单位间的量值关系,建立实现量值转... 基于PCR技术测定的转基因成分定量结果主要是拷贝数比值,而“产品标识”规定检测结果必须以质量分数表示。量值单位的不统一,造成无法根据检测结果直接进行标识与否的判定。因此亟须研究转基因含量2种单位间的量值关系,建立实现量值转换的“转换系数”定值方法,从而保障检测结果与标识阈值的直接可比。通过对国际转基因“产品标识”制度进行总结,系统分析了影响“转换系数”量值的关键因素和欧盟建立的定值方法,并就我国开展相关研究的重要性进行了论述,为我国转基因“产品标识”制度的有效执行提供参考。 展开更多
关键词 生物计量 转基因产品 标准物质 转换系数 数字PCR 产品标识制度
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