The Effects of UV-B Radiation on Maize at the Seedling Stage

In this study, maize plant seedlings were grown in soilless cultures, with and without ^-B radiation at a close of 5.4 kJt（m2-d）. Three clays later, it was found that the treatment of UV-B radiation significantly reduced such characteristics as biomass, plant height, leaf area, and so on. Also, the photosynthesis of the maize was inhabited, the anti-oxid activity decreased, and the MDA concentration increased with the UV-B radiation exposure. The maize presented visible hybrid vigor under the control condition, while the hybrid vigor under UV-B radiation barely emerged in biomass, plant height, leaf area, and physiological metabolic index.

UV-B增强后秸秆还田分解对土壤氮转化微生物及酶活性的影响

为明确UV-B辐射增强对水稻秸秆化学成分的影响,阐释UV-B辐射增强后秸秆还田分解特征及其对稻田土壤氮素转化的间接效应,本研究在元阳梯田(海拔1600 m)开展大田试验,以当地水稻品种白脚老粳为研究对象,研究UV-B辐射增强(5.00kJ·m^(-2))对水稻秸秆化学成分及其还田后秸秆降解、土壤氮素转化的影响。结果表明:UV-B辐射增强显著降低水稻秸秆纤维素含量,增加木质素含量,提高秸秆木质素/氮;并导致秸秆纤维素、木质素、总氮的降解速率总体降低,最大降幅分别达38.7%、18.1%、25.8%。与自然光照秸秆相比,UV-B辐射后的秸秆还田显著降低土壤固氮细菌、氨化细菌、硝化细菌和反硝化细菌数量,增加土壤蛋白酶、氨单加氧酶、硝酸还原酶活性,提高土壤硝化和反硝化速率。相关性分析表明,秸秆木质素/氮与秸秆降解速率呈极显著负相关;秸秆纤维素、木质素、总氮降解速率与硝酸还原酶活性呈显著正相关,后者又与N_(2)O排放通量呈显著正相关;硝化细菌数量与NO_(3)^(-)-N含量呈负相关。研究表明,UV-B辐射增强通过提高秸秆木质素/氮,抑制秸秆纤维素、木质素、总氮降解,减少土壤氨化细菌数量,增加氨单加氧酶和硝酸还原酶活性,从而促进土壤NH_(4)^(+)-N向NO_(3)^(-)-N转化,导致N2O排放通量增加。

彩色马铃薯响应UV-B辐射的转录组分析

[目的]探究彩色马铃薯对UV-B辐射具有较强耐受性的机理,通过转录组分析马铃薯对UV-B辐射响应的分子机制,为筛选马铃薯耐UV-B辐射相关基因提供理论依据。[方法]以彩色马铃薯‘花心洋芋’为试验材料,对全生育期进行UV-B辐射处理,选取成熟期的块茎,采用Illumina HiseQ2500平台进行测序分析。[结果]共得到232 285 320个有效测序读长数,且与参考基因组比对率超过78.5%,GC含量均超过42.1%。通过分析筛选到843个差异表达基因(DEGs),包括544个上调基因和299个下调基因。GO功能分析表明,‘花心洋芋’的DEGs主要富集在代谢过程、细胞组分和催化活性。KEGG富集分析发现,‘花心洋芋’主要通过代谢途径中的碳水化合物代谢通路来响应UV-B辐射,转录因子主要是ARR-B、AP2-EREBP、MADS、bHLH和NAC等,与响应光信号和环境胁迫相关。[结论]‘花心洋芋’响应UV-B辐射的主要通路为代谢途径中的碳水化合物代谢通路,转录因子ARR-B、AP2-EREBP、MADS、bHLH和NAC均起到一定的调控作用。

Increasing Surface UV Radiation in the Tropics and Northern Mid-Latitudes due to Ozone Depletion after 2010

Excessive exposure to ultraviolet(UV)radiation harms humans and ecosystems.The level of surface UV radiation had increased due to declines in stratospheric ozone in the late 1970s in response to emissions of chlorofluorocarbons.Following the implementation of the Montreal Protocol,the stratospheric loading of chlorine/bromine peaked in the late 1990s and then decreased;subsequently,stratospheric ozone and surface UV radiation would be expected to recover and decrease,respectively.Here,we show,based on multiple data sources,that the May–September surface UV radiation in the tropics and Northern Hemisphere mid-latitudes has undergone a statistically significant increasing trend[about 60.0 J m^(–2)(10 yr)^(–1)]at the 2σlevel for the period 2010–20,due to the onset of total column ozone(TCO)depletion[about−3.5 DU(10 yr)^(–1)].Further analysis shows that the declines in stratospheric ozone after 2010 could be related to an increase in stratospheric nitrogen oxides due to increasing emissions of the source gas nitrous oxide(N_(2)O).

青稞BBX基因家族鉴定及其对UV-B的响应

B-box(BBX)蛋白是植物中一类重要的转录因子,在控制植物生长发育、响应环境胁迫中起着重要作用。本研究从青稞(Hordeum vulgare L.var.nudum Hook.f.)全基因组中鉴定出18个青稞BBX基因家族成员,分析了它们的理化性质、基因结构、进化关系及UV-B照射幼苗后的表达特性等。结果表明:青稞BBX基因的编码序列(CDS)长度为636~2 223 bp;分子质量为22.07~54.29 kDa。系统发育分析将18个HvnBBX分为4个亚类。共线性分析表明青稞种内共产生4对片段复制形成的基因对,种间与玉米进化关系更近;HvnBBX启动子区域包含与逆境胁迫、激素、生长发育等相关顺式作用元件。转录组测序结果显示UV-B照射后青稞叶片中有5个BBX基因(HvnBBX2/4/5/6/17)的表达量显著下调,qRT-PCR验证结果与转录组测序一致。上述结果为深入研究HvnBBX基因的抗紫外功能提供参考。

UV-B辐射对滇黄精幼苗生长、抗性生理及代谢物的影响

为探究滇黄精幼苗对UV-B辐射的生理响应机制,以两年生药用植物滇黄精为材料,在540 mw/cm^(2)的UV-B辐射强度下,以5 d为一个周期,分别测定处理了0~25 d后的滇黄精幼苗的生物量、抗氧化酶活性、MDA、类黄酮的含量.结果表明:在UV-B辐射下,滇黄精幼苗的株高与叶绿素含量呈下降趋势,并随着辐射时间延长,影响效果更明显,UV-B辐射对POD、SOD活性的影响呈升高趋势,在UV-B辐射中期,相较于CK显著升高.对MDA含量的影响呈现先升高后降低的趋势.滇黄精叶片中类胡萝卜素含量及叶片和块茎中类黄酮含量也随着辐射时间的延长而增加.因此,UV-B辐射处理可抑制滇黄精幼苗的生长,使其抗氧化酶活性以及类黄酮含量显著增加,从而增强滇黄精对UV-B辐射的抗性,对植株起到保护作用.

过表达AtAUR1对UV-B胁迫下根尖细胞周期调控的影响

随着南北两极臭氧层空洞的持续增大,UV-B辐射对整个地球的生态系统造成的危机不断增强.植物受其特殊生活方式的影响,更容易遭受增强UV-B辐射的影响.UV-B辐射作为一种非生物压力信号会造成植物细胞DNA的损伤从而显著抑制植物生长,严重时甚至会导致植物的死亡.Aurora激酶作为调控有丝分裂的重要蛋白,在有丝分裂过程中参与了染色体的定位、分离以及胞质分裂等过程,实验室之前的研究结果表明过表达AUR1可以缓解UV-B辐射导致的损伤.研究以模式植物拟南芥为材料,通过有性杂交的方法获得含CYCB1;1-GUS标签的拟南芥AUR1过表达株系.通过GUS染色和RT-qPCR的方法对获得的转基因株系在UV-B处理后CYCB1;1的组织表达模式进行了分析,实验结果证明了过表达AUR1可以上调植物根尖细胞中CYCB1;1的基因表达,UV-B辐射处理后的染色结果进一步证明了AUR1参与了UV-B辐射后根尖有丝分裂周期的调控,为揭示过表达AUR1对根尖有丝分裂周期调控的影响提供了一定的理论基础.

Effect of UV-B radiation on the feeding behavior of the rotifer Brachionus plicatilis

Effect of UV - B radiation on the feeding behaviour of marine zooplankton is important to assessing the health harm of marine ecosystem due to the gradually enhanced UV - B radiation in air. However, there are a few studies on this topic. The feeding behavior of the rotifer, Brachionus plicatilis, under the treatment of UV - B radiation on five species of microalgae, i. e. , Chlorella sp. , Tetraselmis chuii, Isochrysis galbana Park 8701, Chaetoceros muelleri Lermumerman, and Nitzschia clostertum, was studied. The results showed that the filtering and feeding rates of the rotifer decreased significantly with the dose increase of UV - B radiation when fed with five species of microalgae respectively （ P 〈 0.05 ） which indicates UV - B radiation inhibits the feeding activities of the rotifer on microalage. The mixed culture experiments shows the rotifer preferred to feed Chlorella sp. , then C. muelleri, L galbana, N. clostertum and T. chuii in turn if without UV - B radiation. Under the highest dose of UV - B radiation treatment （2.70 kJ/m^2）, the rotifer preferred to feed C. muelleri, then Chlorella sp. , N. clostertum, L galbana and T. chuii in turn. Chlorella sp. , L galbana and C. muelleri became the more favorite foods of the rotifer while T. chuii and N. clostertum became less favorite foods. The change of feeding rate and feeding selectivity of zooplankton driven by the enhanced UV - B radiation will lead to the change in the structure of phytoplankton community.

Effects of lanthanum(Ⅲ) on nitrogen metabolism of soybean seedlings under elevated UV-B radiation

The hydroponic culture experiments of soybean bean seedlings were conducted to investigate the effect of lanthanum （La） on nitrogen metabolism under two different levels of elevated UV-B radiation （UV-B, 280-320 nm）. The whole process of nitrogen metabolism involves uptake and transport of nitrate, nitrate assimilation, ammonium assimilation, amino acid biosynthesis, and protein synthesis. Compared with the control, UV-B radiation with the intensity of low level 0.15 W/m^2 and high level 0.45 W/m^2 significantly affected the whole nitrogen metabolism in soybean seedlings （p 〈 0.05）. It restricted uptake and transport of NO3^-, inhibited activity of some key nitrogen-metabolism-related enzymes, such as： nitrate reductase （NR） to the nitrate reduction, glutamine systhetase （GS） and glutamine synthase （GOGAT） to the ammonia assimilation, while it increased the content of free amino acids and decreased that of soluble protein as well. The damage effect of high level of UV-B radiation on nitrogen metabolism was greater than that of low level. And UV-B radiation promoted the activity of the anti-adversity enzyme glutamate dehydrogenase （GDH）, which reduced the toxicity of excess ammonia in plant. After pretreatment with the optimum concentration of La （20 mg/L）, La could increase the activity of NR, GS, GOGAT, and GDH, and ammonia assimilation, but decrease nitrate and ammonia accumulation. In conclusion, La could relieve the damage effect of UV-B radiation on plant by regulating nitrogen metabolism process, and its alleviating effect under low level was better than that under the high one.

The effect of UV-B radiation enhancement on the interspecific competition between Skeletonema costatum and Heterosigma akashiwo

The responses of the interspecific competition between Skeletonema costatum and Heterosigma akashiwo to UV-B radiation enhancement were studied by the co-culture method. The results showed that Heterosigma akashiwo exhibited inhibition on the growth of Skeletonema costatum, and with the increase of initial inoculation density of Heterosigma akashiwo, heavier inhibition on Skeletonema costatum appeared. Under different inoculation proportions, Heterosigma akashiwo could always be in predominance in competition with Skeletonema costatum. The UV-B radiation treatment could change the competition relationship between Skeletonema costatum and Heterosigma akashiwo, which could increase the competitive dominance of Skeletonema costatum and decrease the competitive dominance of Heterosigma akashiwo. When the inoculation proportions of Heterosigma akashiwo and Skeletonema costatum were H∶S=1∶4 and H∶S=1∶1,Skeletonema costatum was in predominance in this competition; however, Heterosigma akashiwo was in predominance when the inoculation proportion was H∶S=4∶1.

Effects of UV-B radiation on the growth interaction of Ulva pertusa and Alexandrium tamarense

Enhanced UV-B(280-320 nm) radiation resulting from ozone depletion is one of global environmental problems. Not only marine organisms but also marine ecosystems can be affected by enhanced UV-B radiation. The effects of UV-B radiation on interaction of macro-algae and micro-algae were investigated using Ulva pertusa Kjellman and Alexandrium tamarense as the materials in this study. The results demonstrated that UV-B radiation could inhibit the growth of Ulva pertusa and Alexandrium tamarense when they were both mono-cultured, and the growth inhibition of algae was more significant with increasing doses of UV-B radiation. Alexandrium tamarense could inhibit the growth of Ulva pertusa in mixed culture, and the growth inhibition was more significant when increasing the initial cell density. However, Ulva pertusa could inhibit the growth of Alexandrium tamarense in early phase and stimulate the growth in latter phase when they were grown in mixed culture. Lower initial cell density(10~2 cell/ml) of Alexandrium tamarense could inhibit the growth of Ulva pertusa under UV-B radiation treatment,however, with the initial cell density increasing(10~3 and 10~4 cell/ml), the growth of Ulva pertusa was stimulated under lower dose of UV-B radiation and inhibited under higher dose of UV-B radiation by Alexandrium tamarense. Compared with that in mixed culture, Ulva pertusa showed more positive inhibition to the growth of Alexandrium tamarense under UV-B radiation treatment.

Effect of UV-B Radiation on Ingesting and Nutritional Selecting Behavior of Rotifer Brachionus urceus

Effect of UV-B radiation on ingesting and nutritional selecting behavior of the roifer Brachionus urceus on 5 species of microlgae were studied under controlled laboratory conditions. Restdts showed that enhanced UV-B radiation significantly inhibited ingesting of the rotifer B. urceus when it was fed with 5 species of micro-algae （p〈0.05）. The ingesting selectivity rate varied with the UV-B radiation enhancement when it was fed with 5 species micro-algal mixture. Results indicated that the enhanced UV-B radiation could affect ingesting and nutritional selectivity of B. urceus.

Saponin synthesis and cotton growth is antagonistically regulated by solar UV-B radiation

Background: Earlier we have reported that the exclusion of solar UV-B increased the growth and yield of cotton plants as compared with ambient UV-B. The UV-B radiation effects on the saponins and the impact of ambient and reduced UV-B on the accumulation of saponins has not been investigated yet. Thus a field experiment was conducted to study the influence of solar UV-B on the growth response and saponin synthesis in cotton(Gossypium hirsutum) var. Vikram plants by the exclusion of UV-B. The cotton plants were grown in specially designed iron chambers, wrapped with filters that excluded UV-B(<315 nm), or transmitted ambient UV-B.Results: Exclusion of ambient UV-B enhanced the growth of cotton plants in terms of plant height and leaf area.Greater plant height in UV-B excluded plants was due to elongated internode and more number of nodes.Enhancement in growth was accompanied by a decrease in the accumulation of saponins, which was quantified by the spectrophotometric, TLC and HPLC methods. Solvent extraction of saponins from the internodes and leaves were used for the bioassay of Amaranthus hypocotyl growth and expansion of cucumber cotyledons. Extracts obtained from the UV-B excluded plants(leaves and internodes) promoted the growth to a larger extent as compared with the extracts obtained from ambient grown plants.Conclusions: The result indicates that solar UV-B may possibly suppress the plant growth by regulating the synthesis of natural growth inhibitor’s like saponin.

Effects of UV-B Radiation and Water Stress on Soybean Yield

Soybean Dongnong 47 was subjected to the experiments of increasing UV-B radiation and water stress on soybean yield components in different growth periods. The results showed that 100-seed weight greatly increased during the early stage of pod filling in the treatment of weak UV-B radiation, seed number per plant as well as seed weight per plant and Dongnong47 yield also increased, while the yield and yield components of Dongnong47 during the blossom to mature period were negatively affected in the treatment of intensive UV-B radiation. 100-seed weight of Dongnong47 all increased in the double factor treatments of UV-B radiation and water stress, with the drought intensified, seed number per plant, seed weight per plant and yield of Dongnong47 decreased, the change of 100-seed weight were various and the antagonistic action of UV-B radiation and water stress were related with their intensity.

Effects of UV-B radiation intensity and timing on epidemiological components of wheat stripe rust

Stripe rust caused by Puccinia striiformis f. sp. tritici is an important wheat disease worldwide that is greatly influenced by environmental conditions. Ultraviolet B(UV-B) radiation is one important environmental factor affecting the occurrence and epidemiology of wheat stripe rust. Investigating UV-B radiation effects on the epidemiology of stripe rust may be conducive to monitoring and predicting this disease. In this study, wheat seedlings were exposed to UV-B radiation during different periods under laboratory conditions and radiation effects on epidemiological components of wheat stripe rust were investigated. Results showed that incubation period was shortened, and the infection efficiency, sporulation quantity and disease index increased when UV-B radiation was performed only pre-inoculation. When the UV-B radiation was performed only postinoculation or both pre-and post-inoculation, the incubation period was prolonged, and the infection efficiency, sporulation quantity and disease index were reduced. When healthy wheat seedlings were inoculated using urediospores collected from wheat leaves irradiated by UV-B only post-inoculation or both pre-and post-inoculation, infection efficiency, sporulation quantity and disease index were also reduced. However, in the latter, the disease incubation period did not differ under varying UV-B radiation intensities compared to that when wheat leaves were not treated with UV-B radiation. Overall, the effects of direct exposure of wheat plants to UV-B radiation with different intensities in different periods on epidemiological components of wheat stripe rust were systematically explored, and the results suggest that the effects of UV-B radiation increased gradually with the increase of UV-B radiation intensity. This information provides a basis for monitoring and predicting this disease as well as for conducting further studies on pathogen virulence variation.

Effect of atmospheric aerosols on UV-B radiation reaching the ground

Solar ultraviolet radiation reaching the ground can be reduced due to light scattering of atmospheric aerosols. Aerosol pollution has led to the decrease in biological active UV-B radiation by about 45% and 10% in city and rural areas, respectively. In populated areas, effect of aerosol scattering on UV-B radiation may offset the increased amount of UV-B caused by ozone depletion, but in clean areas such as two poles, ozone depletion may have great damage effects on ecosystems.

The effects of enriched C0_(2) and enhanced UV-B radiation on ultrastructure of Dunaliella salina, singly and in combination

The effects of ambient CO2/ambient UV-B, enriched CO2/ambient UV-B, ambient CO2/enhanced UV-B, and enriched CO2/enhanced UV-B on the ultrastructure of Dunaliella salina were investigated. （1） The ultrastructure of D..salina cell in the control experiment showed that the arrangement of thylakoid lamellae was regular, and there were many large starch grains among the thylakoid lamellae. A prominent well-developed pyrenoid was found in the middle of the chloroplast. Nucleus envelope and nucleolus were clearly observed. The Golgi apparatus accompanied by numerous vesicles with a compact arrangement of cisternae and the peripheral tips of the cisternae were swollen to a size comparable to that of some of the associated vesicles. （2） The ultrastructure of D. salina cell in enriched CO2 showed that the arrangement of thylakoid was regular and the lamellae were vivid. Developed pyrenoids were found in the low-CO2-grown cells, but not in the high-CO2-grown cells. The mitochondria cristae were vivid. The arrangement of Golgi apparatus was compact. （3） The ultrastructure of D. salina cell in enhanced UV-B showed that the thylakoid was dissolved and the cells had a less developed pyrenoid or no detectable pyrenoid. Part of the nucleus envelope was dissolved. The number ofmitochondria was increased and some mitochondria cristae were disintegrated. The starch grains were broken apart into many small starch grains. The Golgi apparatus with a loose arrangement ofcistemae and the peripheral tips of the Golgi cistemae were not especially swollen, with several large associated vesicles. （4） The ultrastructure of D. salina cell in the enriched CO2/enhanced UV-B showed that part of the thylakoid and nucleus envelopes of some cells were dissolved. The pyrenoid was larger than that of the enhanced UV-B. There were many mitochondria between stroma and chloroplast membrane, but mitochondria cristae were partly dissolved. Many small starch grains were accumulated in cells. The starch sheath was broken into several discontinuous starch grains with different sizes. The arrangement of Golgi apparatus was loose. Above all, although the enriched CO2 can alleviate the damage induced by the UV-B radiation, the effects of experimental UV-B radiation were larger than the effects of actual UV-B radiation, the damage induced by the UV-B radiation was so severe, therefore, CO2 enrichment could not restore the ultrastructure to the control level.

Effects of UV-B radiation and different light repair conditions on the early development of the tetraspores of Chondrus ocellatus Holm

The effects of exposure to UV-B radiation, 280-315 nm, and dark or dark repair conditions on the early development of Chondrus ocellatus Holm collected from Qingdao coast were studied under the laboratory condition. After being exposed to different doses [0, 36, 72, 108, 144 and 180 J/（m2.d）] of UV-B radiation, one treatment of tetraspores were put back to normal culture condition （PAR）, the other treatment were put into dark condition to repair for 2 h. During the cultivation, the diameter of the tetraspores were recorded every 4 d till the 50th day when vertical branches formed in all treatments. Then at the 50th day, CPDs, phycoerythrin, chlorophyll a and MAAs （Palythine and asterina-330） were measured. The results showed that low doses UV-B radiation could significantly accelerate the growth rate of the rate reduced gradually with UV-B radiation keep on tetraspores of C. ocellatus and the growth increasing. The variation trend of both the phycoerythrin and chlorophyll a concentration reduced significantly （P 〈0.05） once given the UV- B radiation. Under UV-B stress, the CPDs were induced, and the concentrations of CPDs were significant low in dark repair groups. The data of MAAs analyzed by LC/MS in the tetraspores of C. ocellatus suggested that there were two MAAs formed （palythine and asterina-330） after UV-B radiation induced, and low dose of UV-B irradiation could significantly induced the concentration of MAAs. From the growth and development state of the tetraspores, negative effects of UV-B radiation on the dark repair groups were more serious than PAR.

Changes of proteins in the Antarctic ice microalga Chlamydomonas sp. cultured under UV-B radiation stress

Antarctic ice microalga Chlamydomonas sp. can thrive undisturbed under high UV radiation in the Antarctic ice layer. However, it is unknown that the initial adaptation mechanisms in protein level occurring in response to high UV radiation. Global-expression profiling of proteins in response to stress was analyzed by two-dimensional electrophoresis （2-DE） and image analysis. In the 2-DE analysis, protein preparation is the key step. Three different protein extract methods were compared, and the results showed that the trichloroacetic acid （TCA）-acetone fractional precipitation method was the fittest one. At the same time, the proteins in Chlamydomonas sp. were compared in 2-DE way, and the synthesis of seven protein spots was found disappeared and 18 decreased after exposed to UV-B radiation. In addition, 14 protein spots were enhanced or induced, among which two new peptides （20 and 21 kDa） appeared whose isoelectric point （pI） was 7.05 and 4.60 respectively. These changed proteins might act as key role in the acclimation of Antarctic ice microalga to UV-B radiation

Effect of UV-B radiation on the population dynamics of the rotifer Brachionus urceus

The effect of UV-B radiation enhancement at experimental doses of 0.00,0.24,0.48,0.72,0.96,and 1.20 kJ/m 2 on the population dynamics of the rotifer Brachionus urceus feeding on the alga Chlorella sp.was studied under controlled laboratory conditions using clonal culture and life-table techniques.The results show that UV-B radiation treatment significantly shortened the survival time of the rotifer （P 0.05）： The longest survival time （336 h） occurred in the control （0.00 kJ/m 2 ） while the shortest （222 h） occurred when exposed to the highest UV-B radiation treatment （1.20 kJ/m 2 ）.The fecundity of the rotifer B.urceus was enhanced by the lower UV-B radiation treatment （0.24 and 0.48 kJ/m 2 ） but inhibited by the higher treatments （0.96 and 1.20 kJ/m 2 ）.Life expectancy also was obviously affected by UV-B radiation （P 0.05）.The highest life expectancy occurred in the control （0.00 kJ/m 2 ）,and it became shorter with increasing doses of UV-B radiation.The resutls in the present study indicate that the life expectancy of the rotifer B.urceus can be used as an indicator for UV-B radiation enhancement.