Penicillium simplicissimum was cultured and preserved on the potato dextrose agar(PDA)medium.PDA-RBBR(Remazal Brilliant Blue R)medium was used for the screening of the strains,which is able to produce enzymes.After th...Penicillium simplicissimum was cultured and preserved on the potato dextrose agar(PDA)medium.PDA-RBBR(Remazal Brilliant Blue R)medium was used for the screening of the strains,which is able to produce enzymes.After the mutation process in Penicillium simplicissimum induced by chemical reagent and ultraviolet radiation,a high laccase-producing strains Penicillium simplicissimum was obtained.When 5 m L diethyl sulfate(2%)was mixed along with 5 m L spore suspension for 30 min,chemical mutagenesis reached its best condition.And the optimum conditions of UV mutagenesis were that spore suspension was irradiated for 4 min under 15 W UV lamp at a distance of 30 cm.The highest activity of C_5E_4 strains was 4.80 U/g over 18%higher than the maximum laccase activity of original microorganism.Five generations of the mutant strains were cultured,and the laccase activity of the strains was measured.The result showed that C_5E_4 strains can product laccase of the five subcultures stably.展开更多
基金Projects(51178172,51308076,51408206,51578222)supported by the National Natural Science Foundation of ChinaProject(113049A)supported by the Ministry of Education of China
文摘Penicillium simplicissimum was cultured and preserved on the potato dextrose agar(PDA)medium.PDA-RBBR(Remazal Brilliant Blue R)medium was used for the screening of the strains,which is able to produce enzymes.After the mutation process in Penicillium simplicissimum induced by chemical reagent and ultraviolet radiation,a high laccase-producing strains Penicillium simplicissimum was obtained.When 5 m L diethyl sulfate(2%)was mixed along with 5 m L spore suspension for 30 min,chemical mutagenesis reached its best condition.And the optimum conditions of UV mutagenesis were that spore suspension was irradiated for 4 min under 15 W UV lamp at a distance of 30 cm.The highest activity of C_5E_4 strains was 4.80 U/g over 18%higher than the maximum laccase activity of original microorganism.Five generations of the mutant strains were cultured,and the laccase activity of the strains was measured.The result showed that C_5E_4 strains can product laccase of the five subcultures stably.
