Objective:To develop an interference-free and rapid method to elucidate GuanxinⅡ(GXⅡ)'s representative vasodilator absorbed bioactive compounds(ABCs)among enormous phytochemicals.Methods:The contents of ferulic ...Objective:To develop an interference-free and rapid method to elucidate GuanxinⅡ(GXⅡ)'s representative vasodilator absorbed bioactive compounds(ABCs)among enormous phytochemicals.Methods:The contents of ferulic acid,tanshinol,and hydroxysafflor yellow A(FTA)in GXⅡ/rat serum after the oral administration of GXⅡ(30 g/kg)were detected using ultra-performance liquid chromatography-mass spectrometry.Totally 18 rats were randomly assigned to the control group(0.9%normal saline),GXⅡ(30 g/kg)and FTA(5,28 and 77 mg/kg)by random number table method.Diastolic coronary flow velocity-time integral(VTI),i.e.,coronary flow or coronary flow-mediated dilation(CFMD),and endothelium-intact vascular tension of isolated aortic rings were measured.After 12 h of exposure to blank medium or 0.5 mmol/L H_(2)O_(2),endothelial cells(ECs)were treated with post-dose GXⅡof supernatant from deproteinized serum(PGSDS,300μL PGSDS per 1 m L of culture medium)or FTA(237,1539,and 1510 mg/m L)for 10 min as control,H_(2)O_(2),PGSDS and FTA groups.Nitric oxide(NO),vascular endothelial growth factor(VEGF),endothelin-1(ET-1),superoxide dismutase(SOD),malondialdehyde(MDA)and phosphorylated phosphoinositide 3 kinase(p-PI3K),phosphorylated protein kinase B(p-AKT),phosphorylated endothelial nitric oxide synthase(p-e NOS)were analyzed.PGSDS was developed as a GXⅡproxy of ex vivo herbal crude extracts.Results:PGSDS effectively eliminates false responses caused by crude GXⅡpreparations.When doses equaled the contents in GXⅡ/its post-dose serum,FTA accounted for 98.17%of GXⅡ-added CFMD and 92.99%of PGSDS-reduced vascular tension.In ECs,FTA/PGSDS was found to have significant antioxidant(lower MDA and higher SOD,P<0.01)and endothelial function-protective(lower VEGF,ET-1,P<0.01)effects.The increases in aortic relaxation,endothelial NO levels and phosphorylated PI3K/Akt/e NOS protein induced by FTA/PGSDS were markedly abolished by NG-nitro-L-arginine methyl ester(L-NA,e NOS inhibitor)and wortmannin(PI3K/AKT inhibitor),respectively,indicating an endothelium-dependent vasodilation via the PI3K/AKT-e NOS pathway(P<0.01).Conclusion:This study provides a strategy for rapidly and precisely elucidating GXⅡ's representative in/ex vivo cardioprotective absorbed bioactive compounds(ABCs)-FTA,suggesting its potential in advancing precision ethnomedicine.展开更多
Volatile organic compounds(VOCs)are difficult to be eliminated safely and effectively because of their large concentration fluctuations.Thus,maintaining a stable concentration of VOCs is a significant study.In this re...Volatile organic compounds(VOCs)are difficult to be eliminated safely and effectively because of their large concentration fluctuations.Thus,maintaining a stable concentration of VOCs is a significant study.In this research,H2O,Tween-80,[Emim]BF4,[Emim]PF6,and[Hnmp]HSO4 were applied to absorb and desorb simulated VOCs.The ionic liquid[Emim]BF4 demonstrated the best performance and was thus selected for further experiments.As the ionic liquid acted as a buffer,the toluene concentration with a fluctuation of 2000–20000 mg·m-3 was stabilized at 6000–12000 mg·m-3.Heating distillation(90°C)was highly efficient to recover[Emim]BF4 from toluene.The regenerated[Emim]BF4 could retain its initial absorption capacity even after multiple cycles.Moreover,[Emim]BF4 had the same buffer function on various aromatic hydrocarbons.展开更多
Intermetallic compounds of AB 5 type are promising materials for M-H batteries. In this report we present the results about the influence of quality of intermetallic compounds prepared by a new method of synthesis on...Intermetallic compounds of AB 5 type are promising materials for M-H batteries. In this report we present the results about the influence of quality of intermetallic compounds prepared by a new method of synthesis on their electrochemical properties. The well-known intermetallic Ln 1-xM xNi 5-yMe y (Ln=La, Mm; M=Zr; Me=Mn, Ge, Sn, Al, Co+Sn, Co+Ge) compounds were synthesized by using mixtures of oxides, chlorides and carbonates of metals by interaction with lithium hydride at 700～1000 ℃. Prepared samples have the uniform microstructure with average dimension of particles about 20～30 μm. Electrochemical tests show that kinetic behaviour of compositions are satisfactory under current up to 200 mA·g -1. These compositions require practically no activation and limiting values of the discharge capacity were reached at 2～3 cycle up to 300 mAh·g -1, which can be considered as most promising for practice.展开更多
A Y-type hexaferrite rod with the composition of Ba2COl.8Cuo.2Fe12022 was presented as an absorbing material with high absorb- anee. Its high absorbance and wide absorption band result from ferromagnetic resonance （...A Y-type hexaferrite rod with the composition of Ba2COl.8Cuo.2Fe12022 was presented as an absorbing material with high absorb- anee. Its high absorbance and wide absorption band result from ferromagnetic resonance （FMR） that is self-biased by strong shape and mag- netocrystaUine anisotropy fields. Around the FMR frequency the specimen of the ferrite rods exhibits very high absorbance and the FMR frequency can be tuned by the rod dimension. In addition to the high absorbance and the wide tunable absorption band, the microwave ab- sorber has another advantage of light weight due to the use of the ferrite rods instead of ferrite slabs.展开更多
Objective： To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment. Methods： Anthraquinones in water extract of rhubarb ...Objective： To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment. Methods： Anthraquinones in water extract of rhubarb （WER）, in cerebrospinal fluid （CSF） of patients with traumatic brain injury （TBI） and in ipsilateral cortex of TBI rats following oral WER were respectively explored by ultra performance liquid chromatography with photodiode array detector （UPLC-PDA） method developed in the present study. The effects of anthraquinones absorbed into injured cortex on superoxidase dismutase （SOD） activity in TBI rats were detected. The antioxidative anthraquinones absorbed into target organ were evaluated for quality control of WER. Results： Anthraquinones in WER were aloe-emodin, rhein, emodin, chrysophanol, and physcion. Only the last anthraquinone was found in CSF and in ipsilateral cortex under this chromatographic condition. Physcion increased SOD activity in TBI rats significantly. Conclusions： Physcion was the main active compound of rhubarb against craniocerebral injury via antioxidant pathway. According to our strategy, the exploration of physcion suggested the possibility of a novel quality control of WER in treating TBI injury.展开更多
基金Supported by the National Natural Science Foundation of China (No.81973589)。
文摘Objective:To develop an interference-free and rapid method to elucidate GuanxinⅡ(GXⅡ)'s representative vasodilator absorbed bioactive compounds(ABCs)among enormous phytochemicals.Methods:The contents of ferulic acid,tanshinol,and hydroxysafflor yellow A(FTA)in GXⅡ/rat serum after the oral administration of GXⅡ(30 g/kg)were detected using ultra-performance liquid chromatography-mass spectrometry.Totally 18 rats were randomly assigned to the control group(0.9%normal saline),GXⅡ(30 g/kg)and FTA(5,28 and 77 mg/kg)by random number table method.Diastolic coronary flow velocity-time integral(VTI),i.e.,coronary flow or coronary flow-mediated dilation(CFMD),and endothelium-intact vascular tension of isolated aortic rings were measured.After 12 h of exposure to blank medium or 0.5 mmol/L H_(2)O_(2),endothelial cells(ECs)were treated with post-dose GXⅡof supernatant from deproteinized serum(PGSDS,300μL PGSDS per 1 m L of culture medium)or FTA(237,1539,and 1510 mg/m L)for 10 min as control,H_(2)O_(2),PGSDS and FTA groups.Nitric oxide(NO),vascular endothelial growth factor(VEGF),endothelin-1(ET-1),superoxide dismutase(SOD),malondialdehyde(MDA)and phosphorylated phosphoinositide 3 kinase(p-PI3K),phosphorylated protein kinase B(p-AKT),phosphorylated endothelial nitric oxide synthase(p-e NOS)were analyzed.PGSDS was developed as a GXⅡproxy of ex vivo herbal crude extracts.Results:PGSDS effectively eliminates false responses caused by crude GXⅡpreparations.When doses equaled the contents in GXⅡ/its post-dose serum,FTA accounted for 98.17%of GXⅡ-added CFMD and 92.99%of PGSDS-reduced vascular tension.In ECs,FTA/PGSDS was found to have significant antioxidant(lower MDA and higher SOD,P<0.01)and endothelial function-protective(lower VEGF,ET-1,P<0.01)effects.The increases in aortic relaxation,endothelial NO levels and phosphorylated PI3K/Akt/e NOS protein induced by FTA/PGSDS were markedly abolished by NG-nitro-L-arginine methyl ester(L-NA,e NOS inhibitor)and wortmannin(PI3K/AKT inhibitor),respectively,indicating an endothelium-dependent vasodilation via the PI3K/AKT-e NOS pathway(P<0.01).Conclusion:This study provides a strategy for rapidly and precisely elucidating GXⅡ's representative in/ex vivo cardioprotective absorbed bioactive compounds(ABCs)-FTA,suggesting its potential in advancing precision ethnomedicine.
基金Supported by the Zhejiang University Students Science and Technology Innovation Activity Plan Funding(No.2018R403078).
文摘Volatile organic compounds(VOCs)are difficult to be eliminated safely and effectively because of their large concentration fluctuations.Thus,maintaining a stable concentration of VOCs is a significant study.In this research,H2O,Tween-80,[Emim]BF4,[Emim]PF6,and[Hnmp]HSO4 were applied to absorb and desorb simulated VOCs.The ionic liquid[Emim]BF4 demonstrated the best performance and was thus selected for further experiments.As the ionic liquid acted as a buffer,the toluene concentration with a fluctuation of 2000–20000 mg·m-3 was stabilized at 6000–12000 mg·m-3.Heating distillation(90°C)was highly efficient to recover[Emim]BF4 from toluene.The regenerated[Emim]BF4 could retain its initial absorption capacity even after multiple cycles.Moreover,[Emim]BF4 had the same buffer function on various aromatic hydrocarbons.
文摘Intermetallic compounds of AB 5 type are promising materials for M-H batteries. In this report we present the results about the influence of quality of intermetallic compounds prepared by a new method of synthesis on their electrochemical properties. The well-known intermetallic Ln 1-xM xNi 5-yMe y (Ln=La, Mm; M=Zr; Me=Mn, Ge, Sn, Al, Co+Sn, Co+Ge) compounds were synthesized by using mixtures of oxides, chlorides and carbonates of metals by interaction with lithium hydride at 700～1000 ℃. Prepared samples have the uniform microstructure with average dimension of particles about 20～30 μm. Electrochemical tests show that kinetic behaviour of compositions are satisfactory under current up to 200 mA·g -1. These compositions require practically no activation and limiting values of the discharge capacity were reached at 2～3 cycle up to 300 mAh·g -1, which can be considered as most promising for practice.
基金supported by the National Natural Science Foundation of China (No.50702005)the Natural Science Foundation of the Education Department of Henan Province,China (No.2010A430012)
文摘A Y-type hexaferrite rod with the composition of Ba2COl.8Cuo.2Fe12022 was presented as an absorbing material with high absorb- anee. Its high absorbance and wide absorption band result from ferromagnetic resonance （FMR） that is self-biased by strong shape and mag- netocrystaUine anisotropy fields. Around the FMR frequency the specimen of the ferrite rods exhibits very high absorbance and the FMR frequency can be tuned by the rod dimension. In addition to the high absorbance and the wide tunable absorption band, the microwave ab- sorber has another advantage of light weight due to the use of the ferrite rods instead of ferrite slabs.
基金Supported by the Fund for Key Labo ratory of Traditional Chinese Medicine Gan of State Administration of Traditional Chinese Medicinethe Major Research Plan of the National Natural Science Foundation of China(No.90409010)was partly supported by the Huge Project to Boost Chinese Drug Development(No.2009ZX09304-003)
文摘Objective： To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment. Methods： Anthraquinones in water extract of rhubarb （WER）, in cerebrospinal fluid （CSF） of patients with traumatic brain injury （TBI） and in ipsilateral cortex of TBI rats following oral WER were respectively explored by ultra performance liquid chromatography with photodiode array detector （UPLC-PDA） method developed in the present study. The effects of anthraquinones absorbed into injured cortex on superoxidase dismutase （SOD） activity in TBI rats were detected. The antioxidative anthraquinones absorbed into target organ were evaluated for quality control of WER. Results： Anthraquinones in WER were aloe-emodin, rhein, emodin, chrysophanol, and physcion. Only the last anthraquinone was found in CSF and in ipsilateral cortex under this chromatographic condition. Physcion increased SOD activity in TBI rats significantly. Conclusions： Physcion was the main active compound of rhubarb against craniocerebral injury via antioxidant pathway. According to our strategy, the exploration of physcion suggested the possibility of a novel quality control of WER in treating TBI injury.
