【目的】检测两个绵羊群体中解偶联蛋白1(uncoupling protein 1,UCP1)基因的单核苷酸多态性(single nucleotide polymorphism,SNP),预测和分析碱基改变对蛋白结构和功能的影响。【方法】利用PCR-SSCP结合测序的方法检测UCP1基因编码区的...【目的】检测两个绵羊群体中解偶联蛋白1(uncoupling protein 1,UCP1)基因的单核苷酸多态性(single nucleotide polymorphism,SNP),预测和分析碱基改变对蛋白结构和功能的影响。【方法】利用PCR-SSCP结合测序的方法检测UCP1基因编码区的SNPs,用生物信息学方法分析UCP1蛋白的理化性质和结构。【结果】UCP1基因编码区存在4个SNPs,其中c.214G>A(Val72Met)和c.273C>T位于外显子2,c.624C>T和c.757G>A(Ala253Thr)位于外显子5。进一步分析导致氨基酸改变的c.214G>A和c.757 G>A突变发现,此两个突变只对UCP1蛋白的理化性质和转录因子结合位点有细微改变,未引起UCP1蛋白空间构象的变化,对蛋白表达的影响不大。【结论】基于对人类中两个与肥胖相关的外显子突变型蛋白结构的比较说明,UCP1蛋白结构的改变可能影响其功能。展开更多
为了解兔肉质性状特征,给兔的品种选种选育提供理论依据,选择UCP1基因设计1对引物扩增其第3外显子及第2内含子部分序列,采用DNA池PCR反应,直接测序法快速检测比利时兔、新西兰兔和加利福尼亚兔3个兔品种UCP基因多态性。结果在新西兰兔...为了解兔肉质性状特征,给兔的品种选种选育提供理论依据,选择UCP1基因设计1对引物扩增其第3外显子及第2内含子部分序列,采用DNA池PCR反应,直接测序法快速检测比利时兔、新西兰兔和加利福尼亚兔3个兔品种UCP基因多态性。结果在新西兰兔中发现1个多态性位点(T387C),多态位点对UCP1基因的RNA结构产生影响,其最小自由能由-917.51 k J/mol变为-927.12 k J/mol,而其他2个品种没有检测到该突变。展开更多
解耦联蛋白1(uncoupling protein 1,UCP1)是线粒体内膜的核编码蛋白,主要在棕色脂肪组织(brown adipose tissue,BAT)中表达,在调节能量代谢和线粒体稳态等方面发挥重要作用。UCP1调节BAT线粒体生物合成、线粒体动力学稳态和线粒体自噬,...解耦联蛋白1(uncoupling protein 1,UCP1)是线粒体内膜的核编码蛋白,主要在棕色脂肪组织(brown adipose tissue,BAT)中表达,在调节能量代谢和线粒体稳态等方面发挥重要作用。UCP1调节BAT线粒体生物合成、线粒体动力学稳态和线粒体自噬,而三者的动态平衡有助于线粒体正常功能的发挥。本文主要对UCP1的结构与分布、UCP1调控BAT能量代谢相关机制、UCP1调控BAT线粒体稳态等方面进行综述。展开更多
Uncoupling protein 1(UCP1)is a proton transporter/channel residing on the inner mitochondrial membrane and is involved in cellular heat production.Using immunohistochemistry,we investigated the expression of UCP1 and ...Uncoupling protein 1(UCP1)is a proton transporter/channel residing on the inner mitochondrial membrane and is involved in cellular heat production.Using immunohistochemistry,we investigated the expression of UCP1 and UCP3 in a series of 98 patients with non-small cell lung cancer(NSCLC)treated with surgery.Expression patterns were correlated with histopathological variables,prognosis,and the expression of enzymes/proteins related to cell metabolism.Bronchial epithelium did not express UCP1 or UCP3,while alveolar cells strongly expressed UCP1.In tumors,strong expression of UCP1 and UCP3 was recorded in43/98(43.8%)and 27/98(27.6%)cases,respectively.UCP1 was significantly associated with squamous cell histology(P=0.05),whilst UCP3 was more frequently overexpressed in large cell carcinomas(P=0.08),and was inversely related to necrosis(P=0.009).In linear regression analysis,UCP1 was directly related to markers of glycolysis[hexokinase(HXKII)and phosphofructokinase(PFK1)]and anaerobic glucose metabolism[pyruvate dehydrogenase kinase(PDK1)and lactate dehydrogenase(LDH5)].UCP3 was directly linked with a glucose transporter(GLUT2),monocarboxylate transporter(MCT2),glycolysis markers(PFK1 and aldolase),and with the phosphorylation of pyruvate dehydrogenase(p PDH).Kaplan-Meier survival analysis showed that UCP3 was significantly related to poor prognosis in squamous cell carcinomas(P=0.04).UCP1 and UCP3 are overexpressed in a large subgroup of non-small cell lung tumors and their expression coincides with increased glucose absorption,intensified glycolysis,and anaerobic glucose usage.Whether UCPs are targets for therapeutic interventions in lung cancer is a hypothesis that demands further investigation.展开更多
Objective We aimed to explore how fermented barley extracts with Lactobacillus plantarum dy-1(LFBE) affected the browning in adipocytes and obese rats.Methods In vitro, 3T3-L1 cells were induced by LFBE, raw barley ex...Objective We aimed to explore how fermented barley extracts with Lactobacillus plantarum dy-1(LFBE) affected the browning in adipocytes and obese rats.Methods In vitro, 3T3-L1 cells were induced by LFBE, raw barley extraction(RBE) and polyphenol compounds(PC) from LFBE to evaluate the adipocyte differentiation.In vivo, obese SD rats induced by high fat diet(HFD) were randomly divided into three groups treated with oral gavage:(a) normal control diet with distilled water,(b) HFD with distilled water,(c) HFD with 800 mg LFBE/kg body weight(bw).Results In vitro, LFBE and the PC in the extraction significantly inhibited adipogenesis and potentiated browning of 3T3-L1 preadipocytes, rather than RBE.In vivo, we observed remarkable decreases in the body weight, serum lipid levels, white adipose tissue(WAT) weights and cell sizes of brown adipose tissues(BAT) in the LFBE group after 10 weeks.LFBE group could gain more mass of interscapular BAT(IBAT) and promote the dehydrogenase activity in the mitochondria.And LFBE may potentiate process of the IBAT thermogenesis and epididymis adipose tissue(EAT) browning via activating the uncoupling protein 1(UCP1)-dependent mechanism to suppress the obesity.Conclusion These results demonstrated that LFBE decreased obesity partly by increasing the BAT mass and the energy expenditure by activating BAT thermogenesis and WAT browning in a UCP1-dependent mechanism.展开更多
目的初步探究孕激素和脂联素受体家族成员9(progestin and adipoQ receptor family member 9,PAQR9)对棕色脂肪产热及解偶联蛋白1(uncoupling protein 1,UCP1)表达的影响。方法选取C57BL/6J小鼠的白色脂肪组织(white adipose tissue,WAT...目的初步探究孕激素和脂联素受体家族成员9(progestin and adipoQ receptor family member 9,PAQR9)对棕色脂肪产热及解偶联蛋白1(uncoupling protein 1,UCP1)表达的影响。方法选取C57BL/6J小鼠的白色脂肪组织(white adipose tissue,WAT)、棕色脂肪组织(brown adipose tissue,BAT)及前棕色脂肪细胞系DE2-3和间充质干细胞C3H10T1/2细胞系进行研究。实时荧光定量PCR技术检测不同条件下PAQR9及UCP1的mRNA水平,蛋白免疫印迹法检测UCP1的蛋白质水平。实时荧光定量PCR技术和油红O染色法检测脂肪细胞的成脂分化情况。结果 C57BL/6J小鼠BAT中PAQR9的mRNA水平显著高于WAT。冷刺激诱导下,BAT和腹股沟皮下WAT中PAQR9的mRNA水平显著升高。DE2-3细胞诱导分化成熟即第6天时,PAQR9的mRNA水平显著高表达。在DE2-3细胞中过表达PAQR9,UCP1的mRNA水平显著上调,细胞耗氧量显著增加,而DE2-3细胞成脂分化功能无显著性差异。在DE2-3细胞中敲低PAQR9,UCP1的mRNA水平显著下调。在C3H10T1/2细胞第4~6天脂肪细胞分化成熟时,PAQR9的mRNA水平显著上调,在敲低PAQR9后UCP1蛋白质水平下调,而C3H10T1/2细胞成脂分化功能无显著性差异。结论 PAQR9能够促进棕色脂肪细胞产热,调控产热基因UCP1的表达。展开更多
文摘为了解兔肉质性状特征,给兔的品种选种选育提供理论依据,选择UCP1基因设计1对引物扩增其第3外显子及第2内含子部分序列,采用DNA池PCR反应,直接测序法快速检测比利时兔、新西兰兔和加利福尼亚兔3个兔品种UCP基因多态性。结果在新西兰兔中发现1个多态性位点(T387C),多态位点对UCP1基因的RNA结构产生影响,其最小自由能由-917.51 k J/mol变为-927.12 k J/mol,而其他2个品种没有检测到该突变。
文摘解耦联蛋白1(uncoupling protein 1,UCP1)是线粒体内膜的核编码蛋白,主要在棕色脂肪组织(brown adipose tissue,BAT)中表达,在调节能量代谢和线粒体稳态等方面发挥重要作用。UCP1调节BAT线粒体生物合成、线粒体动力学稳态和线粒体自噬,而三者的动态平衡有助于线粒体正常功能的发挥。本文主要对UCP1的结构与分布、UCP1调控BAT能量代谢相关机制、UCP1调控BAT线粒体稳态等方面进行综述。
文摘Uncoupling protein 1(UCP1)is a proton transporter/channel residing on the inner mitochondrial membrane and is involved in cellular heat production.Using immunohistochemistry,we investigated the expression of UCP1 and UCP3 in a series of 98 patients with non-small cell lung cancer(NSCLC)treated with surgery.Expression patterns were correlated with histopathological variables,prognosis,and the expression of enzymes/proteins related to cell metabolism.Bronchial epithelium did not express UCP1 or UCP3,while alveolar cells strongly expressed UCP1.In tumors,strong expression of UCP1 and UCP3 was recorded in43/98(43.8%)and 27/98(27.6%)cases,respectively.UCP1 was significantly associated with squamous cell histology(P=0.05),whilst UCP3 was more frequently overexpressed in large cell carcinomas(P=0.08),and was inversely related to necrosis(P=0.009).In linear regression analysis,UCP1 was directly related to markers of glycolysis[hexokinase(HXKII)and phosphofructokinase(PFK1)]and anaerobic glucose metabolism[pyruvate dehydrogenase kinase(PDK1)and lactate dehydrogenase(LDH5)].UCP3 was directly linked with a glucose transporter(GLUT2),monocarboxylate transporter(MCT2),glycolysis markers(PFK1 and aldolase),and with the phosphorylation of pyruvate dehydrogenase(p PDH).Kaplan-Meier survival analysis showed that UCP3 was significantly related to poor prognosis in squamous cell carcinomas(P=0.04).UCP1 and UCP3 are overexpressed in a large subgroup of non-small cell lung tumors and their expression coincides with increased glucose absorption,intensified glycolysis,and anaerobic glucose usage.Whether UCPs are targets for therapeutic interventions in lung cancer is a hypothesis that demands further investigation.
基金supported by the National Natural Science Foundation of China [31701598]the National Natural Science Foundation of China [31371760]the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Objective We aimed to explore how fermented barley extracts with Lactobacillus plantarum dy-1(LFBE) affected the browning in adipocytes and obese rats.Methods In vitro, 3T3-L1 cells were induced by LFBE, raw barley extraction(RBE) and polyphenol compounds(PC) from LFBE to evaluate the adipocyte differentiation.In vivo, obese SD rats induced by high fat diet(HFD) were randomly divided into three groups treated with oral gavage:(a) normal control diet with distilled water,(b) HFD with distilled water,(c) HFD with 800 mg LFBE/kg body weight(bw).Results In vitro, LFBE and the PC in the extraction significantly inhibited adipogenesis and potentiated browning of 3T3-L1 preadipocytes, rather than RBE.In vivo, we observed remarkable decreases in the body weight, serum lipid levels, white adipose tissue(WAT) weights and cell sizes of brown adipose tissues(BAT) in the LFBE group after 10 weeks.LFBE group could gain more mass of interscapular BAT(IBAT) and promote the dehydrogenase activity in the mitochondria.And LFBE may potentiate process of the IBAT thermogenesis and epididymis adipose tissue(EAT) browning via activating the uncoupling protein 1(UCP1)-dependent mechanism to suppress the obesity.Conclusion These results demonstrated that LFBE decreased obesity partly by increasing the BAT mass and the energy expenditure by activating BAT thermogenesis and WAT browning in a UCP1-dependent mechanism.
文摘目的初步探究孕激素和脂联素受体家族成员9(progestin and adipoQ receptor family member 9,PAQR9)对棕色脂肪产热及解偶联蛋白1(uncoupling protein 1,UCP1)表达的影响。方法选取C57BL/6J小鼠的白色脂肪组织(white adipose tissue,WAT)、棕色脂肪组织(brown adipose tissue,BAT)及前棕色脂肪细胞系DE2-3和间充质干细胞C3H10T1/2细胞系进行研究。实时荧光定量PCR技术检测不同条件下PAQR9及UCP1的mRNA水平,蛋白免疫印迹法检测UCP1的蛋白质水平。实时荧光定量PCR技术和油红O染色法检测脂肪细胞的成脂分化情况。结果 C57BL/6J小鼠BAT中PAQR9的mRNA水平显著高于WAT。冷刺激诱导下,BAT和腹股沟皮下WAT中PAQR9的mRNA水平显著升高。DE2-3细胞诱导分化成熟即第6天时,PAQR9的mRNA水平显著高表达。在DE2-3细胞中过表达PAQR9,UCP1的mRNA水平显著上调,细胞耗氧量显著增加,而DE2-3细胞成脂分化功能无显著性差异。在DE2-3细胞中敲低PAQR9,UCP1的mRNA水平显著下调。在C3H10T1/2细胞第4~6天脂肪细胞分化成熟时,PAQR9的mRNA水平显著上调,在敲低PAQR9后UCP1蛋白质水平下调,而C3H10T1/2细胞成脂分化功能无显著性差异。结论 PAQR9能够促进棕色脂肪细胞产热,调控产热基因UCP1的表达。
