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微量液相色谱的先驱——UltiMate^(TM)系统——第一个集微量、毛细管和Nano于一体的完整的液相色谱系统
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作者 刘勇建 牟世芬 《环境化学》 CAS CSCD 北大核心 2001年第5期523-524,共2页
UltiMate是第一个非常完整的毛细管液相色谱系统,包括:①FAMOSTM微量自动进样器,②SwitchosTM微柱自动切换装置,③UltiMateTM微量泵、溶剂配置器和UV检测器.
关键词 液相色谱系统 UltiMate^TM系统 环境监测
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Determination of metformin in diabetic rat plasma by an improved ion-pair high-performance liquid chromatography: application to a pharmacokinetic study 被引量:1
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作者 陈烨 李汉青 +7 位作者 许娇娇 酒向飞 邓晨辉 李新刚 李良 徐小晴 周田彦 卢炜 《Journal of Chinese Pharmaceutical Sciences》 CAS 2012年第3期211-218,共8页
An efficient and sensitive ion-pair HPLC-UV method using atenolol as internal standard (IS) was developed and validated for the determination of metformin in the plasma of diabetic rats. Plasma samples were deprotei... An efficient and sensitive ion-pair HPLC-UV method using atenolol as internal standard (IS) was developed and validated for the determination of metformin in the plasma of diabetic rats. Plasma samples were deproteinated with 10% (v/v) perchloric acid. Separation was achieved on a UltimateTM AQ-C18 column (250 mm×4.6 mm, 5 μm) with a mobile phase (pH 5.05) composed of acetonitrile-water (31:69, v/v, containing 0.002 M sodium dodecyl sulfate, 0.0125 M potassium dihydrogen phosphate, 0.015 M triethylamine) at a flow rate of 1.0 mL/min. The calibration curve was linear (r〉0.994) between 7.5 and 4000 ng/mL. The lower limit of quantification (LLOQ) was 7.5 ng/mL. The precision was validated and the relative standard deviation was in the range of 1.87% to 15.70%; the accuracy was between 93.98%-106.89%. The mean recoveries were 95.40% and 95.31% for metformin and IS, respectively. The relative error (RE) of stability at different storage conditions was within ±9.00%. This method was used to determine the concentration-time profile of metformin in diabetic rat plasma following an oral administration of metformin at the dose of 10 mg/kg. Our results indicated that ion-pair HPLC-UV method using UltimateTM AQ-C18 column was effective for the pharmacokinetic studies of high polarity compounds like metformin. 展开更多
关键词 METFORMIN Ion-pair HPLC Diabetic rat plasma PHARMACOKINETICS Ultimate^TM AQ-C 18
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