Objective:To study the correlation between the ultrasonic elastic strain ratio of thyroid cancer and the malignant biological characteristics of cancer cells in the lesions.Methods:A total of 90 patients with thyroid ...Objective:To study the correlation between the ultrasonic elastic strain ratio of thyroid cancer and the malignant biological characteristics of cancer cells in the lesions.Methods:A total of 90 patients with thyroid cancer who accepted surgical treatment in our hospital between March 2015 and September 2016 were selected as the observation group, and 50 patients who received surgery in our hospital during the same period and were with clear pathological diagnosis of thyroid adenoma were selected as the control group. According to the median of ultrasonic elastic strain ratio, the observing group were further divided into high elastic strain ratio group and low elastic strain ratio group (n=45). RT-PCR was used to determine the mRNA expression of proliferation genes, invasion genes and autophagy genes in lesions of all groups of patients.Results: The mean elastic strain ratio of observation group was significantly lower than that of control group. Proliferation gene FOXA1 mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were higher than those of control group while PP4R1 and Smac mRNA expression were lower than those of control group, and as the elastic strain ratio decreased, the FOXA1 mRNA expression increased while PP4R1 and Smac mRNA expression decreased;invasion genes BCORL1, SATB1, Twist1 and CXCR4 mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were higher than those of control group, and as the elastic strain ratio decreased, the BCORL1, SATB1, Twist1 and CXCR4 mRNA expression increased;autophagy genes ARHI, Beclin1, LC3 and PTEN mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were lower than those of control group, and as the elastic strain ratio decreased, the ARHI, Beclin1, LC3 and PTEN mRNA expression decreased.Conclusion:The ultrasonic elastic strain ratio of thyroid cancer is negatively correlated with tumor cell proliferation and invasion activity, and positively correlated with autophagy activity.展开更多
The measurement of surface stresses in surrounding rocks with the use of a relief method of annular hole-drilling was studied by numerical analysis. The stress relief process by hole-drilling was then simulated with t...The measurement of surface stresses in surrounding rocks with the use of a relief method of annular hole-drilling was studied by numerical analysis. The stress relief process by hole-drilling was then simulated with the use of finite element method. The influences of the borehole diameter(d), the initial stresses and the ratio of the initial principle stresses on the variations of the remained stress and the released stress in function of the relief depth(h) were discussed. The relation between the non-dimensional ratio of the released principle strains and that of the initial principle stresses, and the effect of the elastic modulus and the Poisson ratio of the rock mass on the stress relief curves were studied. The results show that the stress relief behavior formulated with the non-dimensional ratio of the released stress and the ratio of h/d is only sensitive to the ratio of the initial principle stresses and the Poisson ratio. The stresses are completely released when h equals 1.6d, and the tensile stresses take place on the bore core surface in the relief measurement process. Finally, a non-complete relief method of annular hole-drilling for measuring surface stress in surrounding rocks is proposed and the procedure is presented.展开更多
目的探究浆细胞性乳腺炎(PCM)患者超声弹性应变率(SR)值与血清雌二醇(E2)、催乳素(PRL)水平、Ki-67阳性表达率相关性及在预后评估应用价值。方法选择PCM女性患者108例,年龄21~44岁,平均年龄31.03岁。均给予抗生素、微波治疗,持续治疗4...目的探究浆细胞性乳腺炎(PCM)患者超声弹性应变率(SR)值与血清雌二醇(E2)、催乳素(PRL)水平、Ki-67阳性表达率相关性及在预后评估应用价值。方法选择PCM女性患者108例,年龄21~44岁,平均年龄31.03岁。均给予抗生素、微波治疗,持续治疗4周。治疗后统计患者出现并发症、复发等不良事件发生例数,并根据预后情况进行分组。均于入院时、治疗4周后行超声弹性图像检查,检测血清E2、PRL水平和Ki-67阳性表达率。对比两组SR、血清E2、PRL水平、Ki-67阳性表达率,应用Pearson分析SR与血清E2、PRL水平、Ki-67阳性表达率相关性,应用Logistic回归分析探究预后影响因素,应用受试者工作特性(ROC)曲线评价SR对PCM患者预后的评估价值。结果根据治疗后出现并发症、复发等预后情况将患者分为预后良好组(n=85)、预后不良组(n=23)。预后良好组入院时、治疗4周后SR低于预后不良组(3.95±0.53 vs 4.21±0.46;t=2.143,P=0.034。2.67±0.38 vs 2.96±0.42;t=3.175,P=0.002);预后良好组入院时、治疗4周后血清E2、PRL水平、Ki-67阳性表达率低于预后不良组(t=3.854、3.303、4.438、3.484,χ^2=4.644、4.849,P<0.05)。经Pearson相关性分析,PCM患者入院时、治疗4周后SR与血清E2、PRL水平、Ki-67阳性表达率呈正相关(P<0.05);Logistic回归分析可知,乳头状态、分期、SR、血清E2、PRL水平、Ki-67阳性表达率是PCM患者预后重要影响因素(P<0.05)。ROC曲线分析显示,治疗4周后SR AUC为0.751,大于入院时SR(0.727);当截断值>2.90时,灵敏度为69.57%,特异度为74.12%。结论PCM患者SR与血清E2、PRL水平、Ki-67阳性表达率呈正相关,在预后评估中具有一定应用价值。展开更多
文摘Objective:To study the correlation between the ultrasonic elastic strain ratio of thyroid cancer and the malignant biological characteristics of cancer cells in the lesions.Methods:A total of 90 patients with thyroid cancer who accepted surgical treatment in our hospital between March 2015 and September 2016 were selected as the observation group, and 50 patients who received surgery in our hospital during the same period and were with clear pathological diagnosis of thyroid adenoma were selected as the control group. According to the median of ultrasonic elastic strain ratio, the observing group were further divided into high elastic strain ratio group and low elastic strain ratio group (n=45). RT-PCR was used to determine the mRNA expression of proliferation genes, invasion genes and autophagy genes in lesions of all groups of patients.Results: The mean elastic strain ratio of observation group was significantly lower than that of control group. Proliferation gene FOXA1 mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were higher than those of control group while PP4R1 and Smac mRNA expression were lower than those of control group, and as the elastic strain ratio decreased, the FOXA1 mRNA expression increased while PP4R1 and Smac mRNA expression decreased;invasion genes BCORL1, SATB1, Twist1 and CXCR4 mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were higher than those of control group, and as the elastic strain ratio decreased, the BCORL1, SATB1, Twist1 and CXCR4 mRNA expression increased;autophagy genes ARHI, Beclin1, LC3 and PTEN mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were lower than those of control group, and as the elastic strain ratio decreased, the ARHI, Beclin1, LC3 and PTEN mRNA expression decreased.Conclusion:The ultrasonic elastic strain ratio of thyroid cancer is negatively correlated with tumor cell proliferation and invasion activity, and positively correlated with autophagy activity.
基金Projects(2013BAB02B01,2013BAB02B03)supported by the National Key Technology R&D Program of ChinaProject(N120801002)supported by the Fundamental Research Funds for the Central Universities of ChinaProject(N20130042110010)supported by the Specialized Research Fund for the Doctoral Program of Higher Education,China
文摘The measurement of surface stresses in surrounding rocks with the use of a relief method of annular hole-drilling was studied by numerical analysis. The stress relief process by hole-drilling was then simulated with the use of finite element method. The influences of the borehole diameter(d), the initial stresses and the ratio of the initial principle stresses on the variations of the remained stress and the released stress in function of the relief depth(h) were discussed. The relation between the non-dimensional ratio of the released principle strains and that of the initial principle stresses, and the effect of the elastic modulus and the Poisson ratio of the rock mass on the stress relief curves were studied. The results show that the stress relief behavior formulated with the non-dimensional ratio of the released stress and the ratio of h/d is only sensitive to the ratio of the initial principle stresses and the Poisson ratio. The stresses are completely released when h equals 1.6d, and the tensile stresses take place on the bore core surface in the relief measurement process. Finally, a non-complete relief method of annular hole-drilling for measuring surface stress in surrounding rocks is proposed and the procedure is presented.
基金supported by the Natural Science Foundation of Hunan Province (S2010J5043)the fund from the Department of Public Health of Hunan Province,P.R.China (B2009-082)
文摘目的探究浆细胞性乳腺炎(PCM)患者超声弹性应变率(SR)值与血清雌二醇(E2)、催乳素(PRL)水平、Ki-67阳性表达率相关性及在预后评估应用价值。方法选择PCM女性患者108例,年龄21~44岁,平均年龄31.03岁。均给予抗生素、微波治疗,持续治疗4周。治疗后统计患者出现并发症、复发等不良事件发生例数,并根据预后情况进行分组。均于入院时、治疗4周后行超声弹性图像检查,检测血清E2、PRL水平和Ki-67阳性表达率。对比两组SR、血清E2、PRL水平、Ki-67阳性表达率,应用Pearson分析SR与血清E2、PRL水平、Ki-67阳性表达率相关性,应用Logistic回归分析探究预后影响因素,应用受试者工作特性(ROC)曲线评价SR对PCM患者预后的评估价值。结果根据治疗后出现并发症、复发等预后情况将患者分为预后良好组(n=85)、预后不良组(n=23)。预后良好组入院时、治疗4周后SR低于预后不良组(3.95±0.53 vs 4.21±0.46;t=2.143,P=0.034。2.67±0.38 vs 2.96±0.42;t=3.175,P=0.002);预后良好组入院时、治疗4周后血清E2、PRL水平、Ki-67阳性表达率低于预后不良组(t=3.854、3.303、4.438、3.484,χ^2=4.644、4.849,P<0.05)。经Pearson相关性分析,PCM患者入院时、治疗4周后SR与血清E2、PRL水平、Ki-67阳性表达率呈正相关(P<0.05);Logistic回归分析可知,乳头状态、分期、SR、血清E2、PRL水平、Ki-67阳性表达率是PCM患者预后重要影响因素(P<0.05)。ROC曲线分析显示,治疗4周后SR AUC为0.751,大于入院时SR(0.727);当截断值>2.90时,灵敏度为69.57%,特异度为74.12%。结论PCM患者SR与血清E2、PRL水平、Ki-67阳性表达率呈正相关,在预后评估中具有一定应用价值。
