Although gas-filled microbubbles with high echogenicity are widely applied in clinical ultrasonography, the micron scale particle size impedes their use in the treatment of solid tumors, which are accessible to object...Although gas-filled microbubbles with high echogenicity are widely applied in clinical ultrasonography, the micron scale particle size impedes their use in the treatment of solid tumors, which are accessible to objects less than several hundred nanometers. We herein propose an unusual approach involving a pH-induced core-shell micelle-to-vesicle transition to prepare ultrasound-sensitive polymeric nanospheres （polymersomes in structure） possessing multiple features, including nanosize, monodispersity, and incorporation of a phase- transitional imaging agent into the aqueous lumen. These features are not achievable via the conventional double-emulsion method for polymersome preparation. The nanospheres were constructed based on a novel triblock copolymer with dual pH sensitivity. The liquid-to-gas phase transition of the imaging agent induced by external low-frequency ultrasound may destroy the nanospheres for a rapid drug release, with simultaneous tissue-penetrating drug delivery inside a tumor. These effects may provide new opportunities for the development of an effective cancer therapy with few adverse effects.展开更多
Recent advancements in biomedical research have underscored the importance of noninvasive cellular manipulation techniques.Sonogenetics,a method that uses genetic engineering to produce ultrasound-sensitive proteins i...Recent advancements in biomedical research have underscored the importance of noninvasive cellular manipulation techniques.Sonogenetics,a method that uses genetic engineering to produce ultrasound-sensitive proteins in target cells,is gaining prominence along with optogenetics,electrogenetics,and magnetogenetics.Upon stimulation with ultrasound,these proteins trigger a cascade of cellular activities and functions.Unlike traditional ultrasound modalities,sonogenetics offers enhanced spatial selectivity,improving precision and safety in disease treatment.This technology broadens the scope of non-surgical interventions across a wide range of clinical research and therapeutic applications,including neuromodulation,oncologic treatments,stem cell therapy,and beyond.Although current literature predominantly emphasizes ultrasonic neuromodulation,this review offers a comprehensive exploration of sonogenetics.We discuss ultrasound properties,the specific ultrasound-sensitive proteins employed in sonogenetics,and the technique’s potential in managing conditions such as neurological disorders,cancer,and ophthalmic diseases,and in stem cell therapies.Our objective is to stimulate fresh perspectives for further research in this promising field.展开更多
基金This work was supported by the National Basic Research Program of China （No. 2015CB755500）, the National Natural Science Foundation of China （Nos. U1401242, 51225305, and 81430038）, the Natural Science Foundation of the Guangdong Province （No. 2014A030312018）, the Guangdong Innovative and Entrepreneurial Research Team Program （No. 2013S086）, and the Macao Science and Technology Development Fund （No. 096/2015/A3）.
文摘Although gas-filled microbubbles with high echogenicity are widely applied in clinical ultrasonography, the micron scale particle size impedes their use in the treatment of solid tumors, which are accessible to objects less than several hundred nanometers. We herein propose an unusual approach involving a pH-induced core-shell micelle-to-vesicle transition to prepare ultrasound-sensitive polymeric nanospheres （polymersomes in structure） possessing multiple features, including nanosize, monodispersity, and incorporation of a phase- transitional imaging agent into the aqueous lumen. These features are not achievable via the conventional double-emulsion method for polymersome preparation. The nanospheres were constructed based on a novel triblock copolymer with dual pH sensitivity. The liquid-to-gas phase transition of the imaging agent induced by external low-frequency ultrasound may destroy the nanospheres for a rapid drug release, with simultaneous tissue-penetrating drug delivery inside a tumor. These effects may provide new opportunities for the development of an effective cancer therapy with few adverse effects.
基金the National Natural Science Foundation of China(No.81771845)the Chongqing Science and Technology Committee,Chongqing,China(No.CSTB2022NSCQ-MSX0812).
文摘Recent advancements in biomedical research have underscored the importance of noninvasive cellular manipulation techniques.Sonogenetics,a method that uses genetic engineering to produce ultrasound-sensitive proteins in target cells,is gaining prominence along with optogenetics,electrogenetics,and magnetogenetics.Upon stimulation with ultrasound,these proteins trigger a cascade of cellular activities and functions.Unlike traditional ultrasound modalities,sonogenetics offers enhanced spatial selectivity,improving precision and safety in disease treatment.This technology broadens the scope of non-surgical interventions across a wide range of clinical research and therapeutic applications,including neuromodulation,oncologic treatments,stem cell therapy,and beyond.Although current literature predominantly emphasizes ultrasonic neuromodulation,this review offers a comprehensive exploration of sonogenetics.We discuss ultrasound properties,the specific ultrasound-sensitive proteins employed in sonogenetics,and the technique’s potential in managing conditions such as neurological disorders,cancer,and ophthalmic diseases,and in stem cell therapies.Our objective is to stimulate fresh perspectives for further research in this promising field.
