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基于16SrDNA分析的浒苔外生细菌多样性研究 被引量:1
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作者 周新倩 杨锐 +1 位作者 吴小凯 黄艳花 《水产学报》 CAS CSCD 北大核心 2016年第1期110-118,共9页
为研究浙江近海浒苔Ulva spp.(Enteromorpha spp.)外生细菌多样性,采用传统的形态学和16S rDNA测序分析的方法,从舟山朱家尖、宁海国华电厂和奉化南沙3个地区分离到可培养的浒苔外生细菌及其周围海水细菌65株。根据细菌菌落特征和革兰... 为研究浙江近海浒苔Ulva spp.(Enteromorpha spp.)外生细菌多样性,采用传统的形态学和16S rDNA测序分析的方法,从舟山朱家尖、宁海国华电厂和奉化南沙3个地区分离到可培养的浒苔外生细菌及其周围海水细菌65株。根据细菌菌落特征和革兰氏染色结果等将分离到的细菌分为26种表型。16S rDNA序列测序比对发现:菌株与不动杆菌属(Acinetobacter)、交替单胞菌属(Alteromonas)、弧菌属(Vibrio)、假交替单胞菌属(Pseudoalteromonas)、微小杆菌属(Exiguobacterium)、芽孢杆菌属(Bacillus)、赤细菌属(Erythrobacter)、微球菌属(Micrococcus)、假单胞菌属(Pseudomonas)、深海杆菌属(Idiomarina)、Phaeobacter、Roseivirgaj和Silicibacter等23个属相应菌株具有较高同源性。对不同地区浒苔外生细菌进行了Shannon-Wiener多样性指数分析。研究表明:(1)宁海国华电厂的浒苔外生细菌多样性最丰富,多样性指数为93.98%;(2)浒苔外生细菌与其生活地区密切相关,其群落组成具有地域差异,其优势类群也不尽相同,但均归属于变形菌门。 展开更多
关键词 浒苔 16S RDNA 外生细菌 多样性
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A Method for Determining <i>Batillaria attramentaria</i>Distribution Using Aerial Balloon Photography and a Vegetation Index Camera: Demonstration at the Yatsu Tidal Flat, Chiba Prefecture
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作者 Yohei Shiraki Toshiyuki Kitazawa +1 位作者 Seongwon Lee Yukiko Senga 《Advances in Remote Sensing》 2018年第1期15-24,共10页
Located in Chiba Prefecture, the Yatsu Tidal Flat is an important stopover for birds migrating between cold regions such as Siberia and warm regions such as Southeast Asia and Australia. Its importance led to its sele... Located in Chiba Prefecture, the Yatsu Tidal Flat is an important stopover for birds migrating between cold regions such as Siberia and warm regions such as Southeast Asia and Australia. Its importance led to its selection in 1993 as the first tidal flat in Japan to be registered under the Convention on Wetlands of International Importance especially as Waterfowl Habitat (the Ramsar Convention). However, the Yatsu Tidal Flat has in more recent years witnessed blooms of Ulva spp. (sea lettuce) and an increase in exotic species such as Batillaria attramentaria (Japanese mud snail) and Mercenaria mercenaria (hard clam), fueling concerns that the increasing spatial domination of the tidal flat by such species and competition with other species for food may drive a decline in the habitat’s self-cleaning capabilities. For this study, we focused on Batillaria attramentaria, which is now so widely distributed in the Yatsu Tidal Flat as to preclude reliable monitoring via aerial photographs or satellite imagery. Accordingly, we tested the utility of a simplified method for obtaining data on the distribution of Batillaria attramentaria by using aerial balloon photography and a vegetation index camera capable of generating NDVI data. Our results show that under certain conditions, this method can indeed be used to determine Batillaria attramentaria distribution. 展开更多
关键词 Batillaria attramentaria ulva spp. NDVI Yatsu Tidal Flat Japan AERIAL BALLOON PHOTOGRAPHY
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