Expression Profile Changes of Genes Involved in Lipid Metabolism Pathway During Liver Regeneration in Mice

[ Objective ] The aim of the research was to study the expression profile changes of genes involved in lipid metabolism pathway during liver regeneration in mice. [ Method] The CCI4 induced mouse model of liver regeneration was established and the total RNA was isolated from liver tissue of mouse. Then the changes of genes involved in lipid metabolism pathway during different stages of liver regeneration were detected through micro-array chip gene technique and their specific functions were also analyzed. [ Result] Dudng the process of liver regeneration, the expression level of 98 genes involved in lipid metabolism pathway changed, which were divided into eight groups according to change trend. In the mass, the expression of genes was inhibited in the early stage and up-regulated in the late phase. And the gene expression associated with fatty acid synthesis pathway was mainly up-regulated while the catabolic pathway did not change significantly. Most of genes involved in bile acid synthesis pathway were suppressed before 4.5 d and up-regulated after 4.5 d or 7 d. [ Conclusion] During the process of liver regeneration, the genes associated with lipid metabolism are expressed in different trends, and this data should provide a specific range of genes for further studying the regulation effect of lipid metabolism related pathway on liver regeneration.

生姜提取物对X射线损伤小鼠肝脏脂质过氧化的影响

[目的]研究生姜提取物对X射线损伤小鼠肝脏脂质过氧化的影响。[方法]分别以高、中、低剂量生姜提取物给小鼠灌胃,30min后给以2Gy的X射线全身照射。观察不同组小鼠肝脏组织抗氧化酶(超氧化物歧化酶SOD、谷胱甘肽过氧化物酶GSH-PX)活性和脂质过氧化产物丙二醛(MDA)含量的变化。同时设阴性对照组和阳性对照组。[结果]高剂量姜液组抗氧化酶活性降低,MDA含量升高;低剂量姜液组抗氧化酶活性升高,MDA含量降低。[结论]中、低剂量的生姜提取物对X射线照射造成的小鼠抗氧化系统损伤具有保护作用。

血浆脂质氢过氧化物含量测定研究

本文介绍一种用FOX法测定血浆中脂质过氧化的初级产物——脂质氢过氧化物(ROOH)的分光光度法。ROOH在稀酸溶液中可氧化Fe^(2+)变为Fe^(3+),后者与二甲酚橙高度结合产生一种蓝紫色的复合物,在分光光度计上于560 nm测定光密度,根据H_2O_2标准曲线可获得血浆中 ROOH值。该法灵敏度高,重复性好,所需设备简单,可望成为研究自由基和脂质过氧化反应的重要方法之一。

临床数据中心的构建与应用

目的建立以患者为中心、实时的、可扩展的、物理的临床数据中心(CDR),支持医院对临床数据的多元化应用。方法根据HL7和电子病历共享文档规范,基于企业总线型集成平台(ESB),通过中心字典对所需求数据进行标准化处理,实现在香港大学深圳医院的临床数据中心(CDR)的构建。结果实现了全院级的临床数据中心,支持对异构的16个系统,105电子病历文档的数据集成,方便了临床人员对患者数据的查询。结论临床数据中心的构建为医院的医疗质量,临床多元化的数据应用提供了有效的支持。