Upper Tract Treatment of Urogenital Fistulas at the National Fistula Treatment Center (CNTF)

Introduction: Urogenital fistula is the existence of an abnormal pathway between a urinary organ and a genital organ. It is a public health problem because of its frequency and social aspect. The aim of this study was to analyse the management of urogenital fistulas by the upper route at the National Fistula Treatment Centre in N’Djamena. Material and Methods: This was a 10-year retrospective descriptive and analytical study from May 2011 to April 2021. The records of all patients who had received fistula treatment during this period were identified and analysed. Results: During the study period 2369 patients were managed for cure of urogenital fistula including 84 by the upper route, i.e. 3.5%. The mean age was 28.5 ± 8.13 years. Loss of urine was the most common reason for consultation (71.4%). Primigravida were represented in 50% (n = 42). The average gestational age was 3.2 ± 2.8 with extremes of 0 to 9 pregnancies. Obstetric aetiology was the most common (92.8%). Ureterovaginal fistulas were the most common anatomoclinical type (36.9%). Uretero-vesical reimplantation was the main surgical procedure (41.7%). Late postoperative follow-up was successful in 85.7% of cases. Conclusion: Urogenital fistulas are common in our practice. The only way to combat this scourge is through prevention through information, education and communication.

Epidemiologic and Therapeutic Aspect of Urogenital Fistula Following Obstetric and Gynecologic Surgeries Repaired at the Nkwen Baptist Hospital

Introduction: Urogenital fistula is an abnormal communication between the urinary and the genital systems. It may occur following vaginal deliveries or following pelvic surgeries. Data concerning urogenital fistula post-surgery is limited and we have noticed in recent years in Sub-Saharan Africa, an increase in the burden of iatrogenic urogenital fistula. Objective: The aim of this study was to assess the epidemiology, clinical profiles and therapeutic aspects of urogenital fistula following obstetric and gynaecologic surgeries repaired at the Nkwen Baptist Hospital Bamenda. Materials/Methods: This was a longitudinal descriptive study with a retrospective and prospective phase carried out at the Nkwen Baptist Hospital for 5 months. However the complete duration of the study was about 17 months. Data was collected using a pretested questionnaire containing socio-demographic information, clinical features, and therapeutic methods used. We used the statistical software SPSS (Social Package for Statistical Sciences) V 26.0 for data analysis. Results: The number of participants in the study was 40. The mean age was 43.5 years (+/- 13.3) ranging from 16 - 74 years. The prevalence of urogenital fistula post obstetric and gynecologic surgeries at the Nkwen Baptist hospital was 64.6%. The most common symptom was urine leakage and the most common surgery that exposed the patient to the urogenital fistula was total abdominal hysterectomy (60%) followed by caesarean section (35%). The different indications for these surgeries were mostly symptomatic leiomyoma (70.8 %) and prolonged labor (64.2%) respectively. The different types of fistula encountered were Vesicovaginal fistula (55%), Ureterovaginal fistula 40% and Vesicoutetrine fistula 5%. The different treatment modalities used were trans abdominal (77.5%) and transvaginal repair (22.5%). The overall repair success rate after one month was 85%. Conclusion: There is high burden of urogenital fistula post surgeries in our setting. Having more specialists trained in obstetric and gynaecologic procedures may help in the prevention of such an event.

HFACS-MI改进模型在危险化学品仓储事故人因分析中的应用

为了研究危险化学品仓储事故的关键人因,通过人因分析与分类系统-煤矿(Human Factors Analysis and Classification System-Mine,HFACS-MI)改进模型从员工的不安全行为、影响不安全行为的前提条件、监督行为、组织影响和企业外部因素5个层次系统地分析65起危险化学品仓储事故,并运用卡方检验和让步比分析了产生不安全行为的原因和各层次间的内在关系。结果表明,外部管理、组织过程、监督不充分、个人因素、技能差错和违规是导致危险化学品仓储事故发生的重要因素。HFACS-MI改进模型上下层级的人为因素间存在显著的因果关系,且主要的危险化学品仓储事故致因路径共有5条,其中“外部管理因素—组织过程漏洞—监督不充分—个人因素—违规”是最关键的致因路径。改进的HFACS-MI模型可以有效分析危险化学品仓储事故人因因素。

无线紫外光MIMO通信系统信道容量估算方法

无线紫外光通信系统易受大气散射等因素的影响,导致通信信道容量估算准确率较低。为此,提出一种无线紫外光多入多出(Multiple-Input Multiple-Output, MIMO)通信系统信道容量估算方法。根据信道特征,构建无线紫外光MIMO通信系统的信道容量估计模型,获取无线紫外光的单次散射传输特性,利用符号补码式的方法改进空时编码,设定高级检测器中符号的接收能耗,使每个符号保持在标准功率数值范围内,完成通信系统信道检测式编码。根据不同发射角和接收角计算无线紫外光通信的路径损耗比,通过泊松随机分布获得最终通信系统信道容量的估算结果。实验结果表明,所提方法的传输速率可高达8 Mbps,误码率始终低于10-3bit/s,估算准确率在90%以上,估计时间低于4 ms,估算效果好,可为通信行业发展提供理论依据。

STEMI患者外周血单个核细胞中MAPK通路与炎症反应及PCI后无复流的关系

目的 研究ST段抬高型心肌梗死(STEMI)患者外周血单个核细胞(PBMCs)中丝裂原活化蛋白激酶(MAPK)通路与炎症反应及经皮冠状动脉介入术(PCI)后无复流的关系。方法 选取郑州市第七人民医院2021年2月至2022年10月进行PCI治疗的STEMI患者和同期进行体检的健康志愿者,分别作为STEMI组(n=92)和对照组(n=100)。采用荧光定量PCR法检测PBMCs中p38MAPK、JNK的表达水平,根据中位数将STEMI组患者分为p38MAPK、JNK高表达和低表达。检测STEMI患者血清ICAM-1、TNF-α、IL-6含量,评估STEMI患者心肌损伤程度及无复流情况。结果 STEMI组患者PBMCs中p38MAPK、JNK的表达水平高于对照组,差异有统计学意义(t=5.386、5.126,P<0.05);STEMI组中p38MAPK、JNK高表达患者的CK-MB峰值、cTnT峰值及ICAM-1、TNF-α、IL-6含量均高于p38MAPK、JNK低表达患者,差异有统计学意义(t=7.143、4.999、9.528、6.805、6.890、6.226、8.132、7.807、7.162、7.588,P<0.05);STEMI组中无复流患者PBMCs中p38MAPK、JNK的表达水平高于血流正常患者,差异有统计学意义(t=5.208、4.240,P<0.05);经ROC曲线分析,PBMCs中p38MAPK、JNK的表达水平对STEMI患者PCI后无复流具有预测价值(P<0.05)。结论 STEMI患者MAPK通路中p38MAPK、JNK的高表达与炎症反应激活、PCI后无复流有关。

基于互信息和MiniRocket网络的CFST脱空识别

为提高钢管混凝土(concrete filled steel tube,CFST)脱空检测的效率和精度,本文提出了一种基于快速傅里叶变换(fast fourier transform,FFT)、互信息(mutual information,MI)和MiniRocket神经网络的智能识别方法。首先,采用FFT将待测CFST敲击声波时域信号转换为频域信号;其次,采用MI建立频域信号与脱空状态的相关性,提取相关性最大的前30个特征建立数据集,避免了复杂的数学运算和冗余信息;建立MiniRocket深度学习网络,通过使用更少的参数量和更小的特征尺寸,提高分类的速度和精度。最后,考察了模型的噪音鲁棒性,并与其他算法、特征提取方法和识别方法进行对比。结果表明,在不同脱空深度和脱空宽度下,所提的方法在100次重复试验中获得了100%的平均预测精度。在高信噪比下,该方法受影响较小。此外,与其他算法、特征提取方法和识别方法相比,本方法具有更好的预测性能。因此,所提出的方法在未来实际CFST结构的智能脱空识别中具有较大的应用潜力。

基于多重生物信息学数据库分析Mis18β在膀胱癌组织中的表达及其临床意义

目的:探讨Mis18β基因在膀胱癌组织中的表达、临床意义及在疾病过程中的作用。方法:通过癌症基因组图谱(The Cancer Genome Atlas, TCGA)数据库获得膀胱癌患者Mis18β mRNA测序数据及临床资料数据,通过基因表达综合(Gene Expression Omnibus, GEO)数据库获得膀胱癌Mis18β表达数据,分析Mis18β在膀胱癌组织和癌旁组织中mRNA表达差异及与患者临床病理参数的相关性并进行生存分析,利用基因本体论(Gene Ontology, GO)功能分析以及京都基因与基因组百科全书(Kyoto Encyclopedia of Gene and Genomes, KEGG)通路富集分析探索Mis18β在膀胱癌发生发展中可能的信号通路。收集膀胱癌患者及健康对照者外周血标本,RT-PCR方法验证Mis18β mRNA表达水平的差异。结果:TCGA数据库和GEO数据库分析显示膀胱癌组Mis18β的表达量明显高于对照组(P<0.05)。生存分析提示Mis18β高表达组膀胱癌患者的总生存期明显低于低表达组(P<0.05)。功能富集分析显示Mis18β主要参与细胞有丝分裂,调控细胞周期及DNA复制等过程。膀胱癌患者外周血有核细胞中Mis18β的含量明显高于对照组(P<0.05)。结论:Mis18β在膀胱癌中呈高表达,其表达水平与患者年龄、病理类型、总生存期等临床病理特征存在相关性,为揭示膀胱癌发生发展的分子机制及寻找新的诊断、预后标志物提供了理论基础。

Urogenital Trauma by Pelvic Impalement: A Case Reported at Bouake University Hospital

Urogenital impalement trauma is a particular form of penetrating trauma to the pelvis. They are rare, spectacular or dramatic because they can be responsible for serious vascular and/or visceral lesions. This study reports a case of hypogastric impalement in a 19-year-old young man that caused musculoaponeurotic and urogenital lesions. Emergency surgical exploration was carried out urgently by a multidisciplinary team.

Antibiotics Resistance of Urogenital Mycoplasma in Sexually Active Women Attending Gynecologic Consultation in Douala (Cameroon)

Introduction: Mycoplasmas are bacteria commonly found in the commensal flora of humans and can occasionally be pathogenic. The population and the geographical area determine their susceptibility to various antibiotics. Methodology: We carried out a cross-sectional study from January to June 2022 at the Douala General Hospital. All women who were sexually active and willing to participate were included. Endo-cervical swabbing was used to collect the samples. Culture and antibiotic susceptibility testing were performed in a liquid medium using Mycoplasma-SystemPlus Gallery (Liofilchem). All samples that degraded urea (threshold > 103 for Ureaplasma urealyticum) and arginine (threshold > 104 for Mycoplasma hominis) were deemed positive. Results: A total of 107 women with a median age of 33 ± 8.3 years and a predominance of 30-40 years (41.1%) were included. The frequency of mycoplasma infection was 73.8% with 34.1%, 11.4% and 54.4% for Ureaplasma urealyticum, Mycoplasma hominis and co-infection respectively. Resistance rates to Minocycline were low, 1.3% for Ureaplasma urealyticum and 3.7% for co-infection;for Pefloxacin the resistance rates were 3.7% (Ureaplasma urealyticum, Mycoplasma hominis) and 22.8% (co-infection). Ureaplasma urealyticum had a resistance rate of 3.7% for Erythromycin, 1.3% for Clarithromycin and 5% for Azithromycin. There was no significant correlation between risk factors and infection. Vaginal cleansing and the development of resistance in mycoplasma infection were found to be significantly correlated (OR = 6.915 [1.52 - 31.55];(p = 0.013)). Conclusion: Minocycline was the most active antibiotic and Ureaplasma urealyticum was the species with the lowest rate of resistance. Antibiotic resistance was more common in co-infected people than mono-infected. Antibiotic resistance was independently correlated with vaginal douching.

Urogenital Trauma: Epidemiological and Diagnostic Aspects at the Borgou University and Departmental Hospital Center (Benin)

Objective: To study the epidemiological and diagnostic aspects of urogenital trauma at the Borgou University and Departmental Hospital Center. Material and Methods: This was a descriptive cross-sectional study that took place over a period of 4 years and 4 months (52 months) from January 1st 2017 to April 30th 2021. All cases of urogenital trauma treated in the General surgery department during the period were collected. Patient records, hospitalization registers and operating reports were used to collect information. Data entry was done using Epi data 3.1 software, French version. The analysis was performed using MedCalc software (version 19.4.1. Mariakerke, Belgium), and Epi info software version 7. Results: In 52 months, 75 cases of urogenital trauma were collected. The prevalence of urogenital trauma was 1.1% of admissions with an annual incidence of 17.3 cases. The modal age class was [20;40], i.e. 49.3%. Males accounted for 76.0% (n = 57) of cases. Urethral and kidney injuries were found in 33.3% and 21.3%, respectively. The external genitalia was involved in 24.0%. Road traffic accidents occupied the first place with 62.6%. Kidney lesions were revealed by lumbar pain in 62.5% (n = 10) and post traumatic hematuria in 25% (n = 4) of cases. Most of the patients (n = 8, i.e. 50%) were classified as grade I according to the AAST classification. Conclusion: Urogenital trauma is common in our environment and can be life-threatening. Knowledge of their epidemiology will allow for prevention and good management.

膀胱癌组织中MIS18BP1的表达及临床意义

目的探讨MIS18BP1在膀胱癌中的表达、与临床病理参数相关性及临床意义。方法TCGA、GEO数据库分析MIS18BP1在肿瘤和对照组中mRNA表达并经qRT-PCR验证;UALCAN在线数据库分析MIS18BP1表达及与临床病理参数、免疫细胞浸润程度相关性;免疫组织化学分析MIS18BP1在膀胱癌中的表达及与临床病理特征的关系;ROC曲线评价MIS18BP1 mRNA对膀胱癌的诊断价值。结果生物信息学分析及qRT-PCR结果显示,与对照组相比较,MIS18BP1 mRNA在膀胱癌中表达增加(P<0.05);免疫组织化学结果表明膀胱癌MIS18BP1蛋白阳性率显著增高(P<0.05),且与肿瘤临床分期、浸润深度及淋巴结转移相关(P<0.05);免疫浸润分析显示MIS18BP1与膀胱癌中免疫细胞浸润相关。结论MIS18BP1基因和蛋白在膀胱癌中表达水平增高,可能通过影响免疫细胞浸润调控膀胱癌的发生发展。

对MIS结构热弛豫时间的分析计算

对MIS(金属-绝缘层-半导体)结构从深耗尽过渡至强反型状态所需的热弛豫时间进行了研究。通过建立较教科书更精确的模型,对热弛豫时间τth与深耗尽时耗尽区宽度x d0、强反型时耗尽区最大宽度x dm、耗尽区内少子净产生率G和掺杂浓度N D等关键物理量之间的关联进行了推导,并基于MEDICI平台对推导结果完成了仿真验证,从而加强了学生对半导体表面效应的理解,也利于他们未来从事半导体方面的创新研究。

血清miR-590联合粪便钙卫蛋白对溃疡性结肠炎的诊断价值及其与内镜下疾病活动度的相关性

目的 探究血清miR-590联合粪便钙卫蛋白(FC)对溃疡性结肠炎(UC)的诊断价值及与内镜下疾病活动度的相关性。方法 选择2021年1月至2023年2月滨州市人民医院消化内科收治的84例UC患者设为研究组,另选择同期在该院体检的56名健康志愿者设为对照组。分别采用实时荧光定量PCR法和ELISA法检测2组的血清miR-590和FC水平。采用Pearson相关性分析法探究血清miR-590与溃疡性结肠炎内镜下严重程度指数(UCEIS)的相关性,采用Spearman秩相关性分析法探究FC与UCEIS的相关性。采用ROC曲线分析血清miR-590联合FC对UC的诊断价值及与内镜下疾病活动度的相关性。结果 研究组的血清miR-590表达水平较对照组显著降低,FC水平较对照组显著升高,差异均有统计学意义(P均<0.05)。血清miR-590联合FC诊断UC的敏感度、特异度、曲线下面积(AUC)分别为95.48%、76.85%、0.914,两者联合的诊断效能均高于单一指标(P均<0.05)。与缓解期UC患者相比,活动期UC患者的血清miR-590表达水平较低,且血清miR-590表达水平与内镜下疾病活动度呈负相关(P<0.05)。与缓解期UC患者相比,活动期UC患者的FC水平较高,且FC水平与内镜下疾病活动度呈正相关(P<0.05)。UC患者的血清miR-590表达水平与UCEIS评分呈显著负相关(r=-0.791,P=0.000),FC水平与UCEIS评分呈显著正相关(r=0.884,P=0.000)。血清miR-590联合FC评估UC内镜下疾病活动度的敏感度、特异度、AUC分别为97.31%、78.42%、0.946,两者联合的评估效能均高于单一指标(P均<0.05)。结论 血清miR-590联合FC有助于提高UC诊断的敏感度,并可作为辅助指标评估内镜下疾病活动度,具有临床推广价值。

具有低噪声及高线性度的高性能MOCVD-SiN_(x)/AlN/GaN毫米波MIS-HEMTs

文章在超薄势垒AlN/GaN异质结构上采用金属有机化学气相沉积(MOCVD)原位生长SiN_(x)栅介质,成功制备了高性能的SiN_(x)/AlN/GaN金属-绝缘体-半导体高电子迁移率晶体管(MIS-HEMTs)。深能级瞬态谱(DLTS)技术测试SiN_(x)/AlN的界面信息,显示其缺陷能级深度为0.236 eV,俘获截面为3.06×10^(-19)cm^(-2),提取的界面态密度为10^(10)~10^(12)cm^(-2)eV^(-1),表明MOCVD原位生长的SiN_(x)可以有效降低界面态。同时器件表现出优越的直流、小信号和噪声性能。栅长为0.15μm的器件在2 V的栅极电压(Vgs)下具有2.2 A/mm的最大饱和输出电流,峰值跨导为506 mS/mm,最大电流截止频率(fT)和最大功率截止频率(fMAX)分别达到了65 GHz和123 GHz,40 GHz下的最小噪声系数(NFmin)为1.07 dB,增益为9.93 dB。Vds=6 V时对器件进行双音测试,器件的三阶交调输出功率(OIP3)为32.6 dBm,OIP3/Pdc达到11.2 dB。得益于高质量的SiN_(x)/AlN界面,SiN_(x)/AlN/GaN MISHEMT显示出了卓越的低噪声及高线性度,在毫米波领域具有一定的应用潜力。

A Clinical Research about the Intermittent Medication Treatment on the Female Urogenital Tract Mycoplasma Infection

Object: To explore a better treatment on the female urogenital tract mycoplasma infection so as to ensure the health of female reproduction. Method: 115 female patients with urogenital tract?mycoplasma infection?are selected, including 63 patients acted as a group for intermittent medication treatment;that is to say, the ones that are confirmed to be sensitive drugs to those 63 patients are chosen, and let them take this medication for 15 days and stop taking it for 7 days and after that continue to take it for 10 days. The other 52 patients in the other group which acts as a contrast to the former group are cured with a course of conventional treatment for 15 - 21 days. Results: As to those 115 female patients with urogenital tract mycoplasma infection, 43 patients get relapsed, and 27 patients of them are in the contrast group and 16 patients are in the treatment group.?There is obvious difference on the contrast of relapse rate between the treatment group and contrast group, and they are P < 0.01. But as to the sensitive testing results of 10 common antibiotic medicines, there are cases of illness of relapse in those 2 groups, and the rate of drug sensitivity in the treatment group is higher than that of the contrast group. Between treatment group and contrast group, there is obvious difference in the sensitivity of the drugs, P < 0.05 average. Conclusion: When the female patients with urogenital tract mycoplasma infection are cured with a traditional treatment, because of the side effects of drugs, they are not likely to take medicines that are required to making the cure rate become lower and the relapse rate become higher. But intermittent medication is designed with the half-life period of medicine, which makes the medicine can be digested and absorbed easily, and the cure rate is high and the relapse rate is low. With this research we found a more effective method of the treatment on the female urogenital tract mycoplasma infection and showed the clinical significance in intermittent medication.

Urogenital Fistula in Cote d’Ivoire: Epidemiological, Clinical and Therapeutical Aspects

Goal: Contribute to improve treatment of patients suffering from urogenital fistula (UGF). Materials and methods: It was a descriptive and prospective study conducted by the United Nations Population Fund (UNFPA) and the Ivorian government, on treatment of fistulas. The caravan took place on 4 periods of 10 days each and has enabled us to recruit 95 patients in the gynecological emergency department of Bouake’s University Hospital Center (UHC) and from the surgical emergencies departments of the regional hospital centers (RHC) of Man and Bondoukou, in Man from 14th to 23rd of August 2013, then from 24th March to 02nd of April 2014;in Bouaké from 06th to 15th of November 2013 and in Bondoukou from 13th to 22nd of December 2013, respectively. Results: Average age of patients was of 30.8 years. 50 patients were single (52.6%). 44.2% of patients were farmers. In 95.8% of cases it was about obstetrical fistulas of which 52.6% were caused by caesareans. Average duration of delivery labor was of 2.3 days. Vesicovaginal fistulas represented 65.3% of UGF. Suture split were carried out on 90 patients (94.7%). Within 01 month, success rate was of 63.9% for 83 known patients. Conclusion: Incidence of UGF in our country is certainly underestimated. They are mainly of obstetrical origin and treatment relies on sutures split which give satisfactory results.

Enhanced Hoek-Brown(H-B)criterion for rocks exposed to chemical corrosion

Underground constructions often encounter water environments,where water–rock interaction can increase porosity,thereby weakening engineering rocks.Correspondingly,the failure criterion for chemically corroded rocks becomes essential in the stability analysis and design of such structures.This study enhances the applicability of the Hoek-Brown(H-B)criterion for engineering structures operating in chemically corrosive conditions by introducing a kinetic porosity-dependent instantaneous mi(KPIM).A multiscale experimental investigation,including nuclear magnetic resonance(NMR),X-ray diffraction(XRD),scanning electron microscopy(SEM),pH and ion chromatography analysis,and triaxial compression tests,is employed to quantify pore structural changes and their linkage with the strength responses of limestone under coupled chemical-mechanical(C-M)conditions.By employing ion chromatography and NMR analysis,along with incorporating the principles of free-face dissolution theory accounting for both congruent and incongruent dissolution,a kinetic chemical corrosion model is developed.This model aims to calculate the kinetic porosity alterations within rocks exposed to varying H+concentrations and durations.Subsequently,utilizing the generalized mixture rule(GMR),the kinetic porositydependent mi is formulated.Evaluation of the KPIM-enhanced H-B criterion using compression test data from 5 types of rocks demonstrated a high level of consistency between the criterion and the experimental results,with a coefficient of determination greater than 0.96,a mean absolute percentage error less than 4.84%,and a root-mean-square deviation less than 5.95 MPa.Finally,the physical significance of the porosity-dependent instantaneous mi is clarified:it serves as an indicator of a rock’s capacity to leverage the confining pressure effect.

基于微服务的企业综合管理信息系统(MIS)设计与实现

当前企业管理离不开相关数据的准确性和实时性,建设基于微服务的企业综合管理信息系统可增强企业管理信息能力,实现各部门整体工作的协同优化。采用微服务架构在设计和开发的过程中可实现服务的快速部署和更改,,SpringCloud框架是微服务架构中的集成,它将一系列优秀的组件进行整合,企业综合管理信息系统采用前后端分离的架构模式,前端基于VUE框架技术搭建,后端采用SpringBoot框架,前端和后端可并行开发,以便于维护和升级。基于微服务的企业综合管理信息系统为企业提供一个便捷、高效的平台,提升管理效率,对企业的快速发展具有重要意义。

STEMI患者超敏C反应蛋白水平与TIMI血流分级及Gensini评分的关系

目的探讨急性ST段抬高型心肌梗死(STEMI)患者的超敏C反应蛋白水平与TIMI血流分级及Gensini评分的关系。方法收集2021年1一12月巴彦淖尔市医院行急诊PCI治疗的STEMI患者288例,根据TIMI血流分级及Gensini评分将研究对象分为A组(TIMI 0~1级186例)和B组(TIMI2~3级102例)、轻度组(Gensini评分≤81分145例)和重度组(Gensini评分>81分143例),比较临床资料,分析hs-CRP水平与STEMI患者PCI术前冠脉血流分级降低及冠状动脉病变程度相关性。结果TIMI 2~3级患者的hs-CRP水平明显低于TIMI 0~1级患者(P=0.01),且Spearman相关系数法分析结果显示血清hs-CRP与术前TIMI血流呈显著负相关(r=-0.174,P<0.01),多因素回归分析的结果表明,hs-CRP是TIMI血流降低的危险因素(OR=0.819,95%CI:0.683~0.982,P<0.05),提示hs-CRP是术前TIMI血流降低的预测因子。轻度组血清hs-CRP水平小于重度组(P<0.05),且Spearman相关系数法分析结果显示hs-CRP与STEMI患者Gensini评分呈正相关(r=0.140,P=0.017),表明患者hs-CRP水平越高,Gensini评分越高。结论本研究发现hs-CRP水平与STEMI患者术前TIMI血流降低及Gensini评分有显著相关性。

Therapeutic Effects and Potential Mechanisms of Glyasperin A against Myocardial Ischemia Based on Network Pharmacology and Molecular Docking

[Objectives]To explore the therapeutic effects and potential mechanisms of Glyasperin A(GAA)on myocardial ischemia(MI)based on network pharmacology and molecular docking.[Methods]The molecular structure of GAA was downloaded from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and all targets of GAA were predicted by converting 3D model molecules into SMILES online tool and Swiss target prediction.Genecards database and DisGeNET database were used to find the targets related to MI,and then Venny 2.1.0 was used to generate the corresponding Wayne diagram,and then Cytoscape 3.9.1 software was used to construct the protein-protein interaction(PPI)network.With the help of DAVID database and Microbiology,the selected core targets were enriched and analyzed by gene ontology(GO),biological process(BP),and Kyoto Encyclopedia of Genes and Genomes(KEGG),and then the molecular docking between GAA and core targets was verified by AutoDock and Pymol software.[Results]A total of 1883 MI targets were screened,and in the protein-protein interaction network,AKT1,PTGS2,PPARG,ESR1,GSK3B were the proteins with higher values.Gene ontology and KEEG enrichment analysis showed that the biological processes involved mainly included inflammatory response,negative regulation of gene expression,and response to exogenous stimuli.Signaling pathways mainly include IL-17 signaling pathway,HIF-1 signaling pathway,and so on.The results of molecular docking showed that the binding energy of GAA and core protein was less than-5 Kcal/mol in four groups.These indicated that GAA with good binding had a certain therapeutic effect on myocardial ischemia.[Conclusions]Based on the systematic network pharmacology method,this study predicts the basic pharmacological effects and potential mechanisms of GAA in the treatment of MI,and reveals that GAA may treat MI through multiple targets and signaling pathways.It is expected to provide a basis for further study of its pharmacological mechanisms.