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亚洲黑熊四川亚种Ursus thibetanus mupinensis线粒体细胞色素C氧化酶亚基Ⅲ基因的克隆与初步分析 被引量:5
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作者 杜玉杰 黎云祥 +2 位作者 吴夏 陈瑜 侯万儒 《四川大学学报(自然科学版)》 CAS CSCD 北大核心 2007年第6期1363-1368,共6页
根据已报道的部分哺乳动物线粒体细胞色素C氧化酶(Cytochrome C Oxidase)亚基Ⅲ基因(mtCOXⅢ)的相关信息设计引物,运用PCR技术,首次从亚洲黑熊四川亚种(以下简称四川黑熊,Ursus thibetanus mupinensis)的肌肉组织总DNA中成功克隆了mtCO... 根据已报道的部分哺乳动物线粒体细胞色素C氧化酶(Cytochrome C Oxidase)亚基Ⅲ基因(mtCOXⅢ)的相关信息设计引物,运用PCR技术,首次从亚洲黑熊四川亚种(以下简称四川黑熊,Ursus thibetanus mupinensis)的肌肉组织总DNA中成功克隆了mtCOXⅢ基因的编码序列,并对其进行了初步分析.结果表明:四川黑熊mtCOXⅢ基因序列全长784 bp,ORF是783 bp,编码261个氨基酸的蛋白质,该蛋白的等电点为6.47,分子量为29840.6 Da.四川黑熊的mtCOXⅢ与已报道的部分哺乳动物的mtCOXⅢ基因具有很高的同源性.进一步分析发现,四川黑熊线粒体细胞色素C氧化酶亚基Ⅲ功能位点的位置、种类和数量均与已报道的部分哺乳动物具有很高的相似性. 展开更多
关键词 亚洲黑熊四川亚种(ursus thibetanus mupinensis) 线粒体 细胞色素C氧化酶亚基Ⅲ(mtCOXⅢ) 克隆 序列分析
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Blood Typing of Asiatic Black Bear (Ursus thibetanus)
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作者 Li Qin Chen Yucun +3 位作者 Zhao Fengyong Zhang Jiamin Zhang Shuhui Zhu Ziyan 《Animal Husbandry and Feed Science》 CAS 2017年第5期322-325,共4页
[Objective]The paper was to analyze whether Asiatic black bear( Ursus thibetanus) had different blood group systems. [Method]Whole blood samples were collected from 40 Asiatic black bears in Fujian Province,China. Tub... [Objective]The paper was to analyze whether Asiatic black bear( Ursus thibetanus) had different blood group systems. [Method]Whole blood samples were collected from 40 Asiatic black bears in Fujian Province,China. Tube method was used to detect antibodies in plasma,and antibody isotype was determined with 2-mercaptoethanol. Plasma was further analyzed by mass spectrometry. [Result] The plasma from four black bears had antibodies,possibly Ig M isoform,which could agglutinate RBCs from 30 bears. Blood samples from 10 bears were tested by human blood typing reagents. The results showed that four black bears had blood type like human type O,while six bears had like human type B. Mass spectrometry results demonstrated that plasma protein had extensive homology to serum albumin-like isoform 1 found in giant panda( Ailuropoda melanoleuca). [Conclusion]Asiatic black bear might have at least one blood group system with two blood types. If the sick bear needs blood transfusion,a cross-matching test is necessary. Moreover,giant panda might receive blood from Asiatic black bear in case of emergency. 展开更多
关键词 Asiatic BLACK BEAR (ursus thibetanus) BLOOD group system Cross matching test TRANSFUSION MEDICINE
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Matrilinear hierarchy in the American black bear (Ursus americanus) 被引量:1
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作者 Benjamin KILHAM James R.SPOTILA 《Integrative Zoology》 SCIE CSCD 2022年第1期139-155,共17页
The American black bear(Ursus americanus)was long thought to be solitary and its social organization has not been well described.Here,we present new data on black bear social structure.The objectives of the study were... The American black bear(Ursus americanus)was long thought to be solitary and its social organization has not been well described.Here,we present new data on black bear social structure.The objectives of the study were to make detailed observations of the behavior of wild black bears to determine their social interactions and structure.We tested whether black bears interacted socially beyond mating and competing for resources,if black bears tracked relationships and interacted regularly even when resources were not limited,and whether the social structure of a population of black bears was based on a matrilinear hierarchy.We collected data by direct observation of bears from 1993 to 2014.Observations of 1210 social interactions at a provisioning site indicated that females compete and form matrilinear hierarchies.Dominant bears established a hierarchy for food,control of space,and control of younger bears.Post interaction scent marking took place,which suggested that dominant females were conditioning subordinates to their scent marks.Affiliative behavior occurred between related and unrelated bears and helped to establish the social structure of the bear community.Based on our data,human–bear conflicts can be reduced by behavioral modifications by humans when they encounter bears.Knowledge of bear behavior and the matrilinear hierarchy provide a basis for non-lethal management of bears that find themselves in a bear–human conflict situation. 展开更多
关键词 BEAR matrilinear hierarchy New Hampshire RANK social behavior ursus
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四川黑熊线粒体12S和16S rRNA基因的克隆及序列分析 被引量:6
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作者 吴夏 陈瑜 +2 位作者 彭正松 杨军 侯万儒 《四川师范大学学报(自然科学版)》 CAS CSCD 北大核心 2007年第3期402-405,共4页
利用哺乳动物线粒体基因的保守序列设计引物,采用PCR方法,首次从亚洲黑熊四川亚种(Ursusthibetanus mupinensis)的肌肉组织总DNA中扩增出了线粒体12S rRNA和16S rRNA基因并进行了序列测定及分析.结果表明,四川黑熊12S rRNA基因长965 bp;... 利用哺乳动物线粒体基因的保守序列设计引物,采用PCR方法,首次从亚洲黑熊四川亚种(Ursusthibetanus mupinensis)的肌肉组织总DNA中扩增出了线粒体12S rRNA和16S rRNA基因并进行了序列测定及分析.结果表明,四川黑熊12S rRNA基因长965 bp;16S rRNA基因长1 580 bp.通过进一步的序列分析表明,四川黑熊的12S rRNA和16S rRNA基因有较高的进化速率,与美洲黑熊、棕熊、北极熊、眼镜熊及大熊猫的相应基因相比较有较大差异,其中与美洲黑熊的同源性相对较高. 展开更多
关键词 亚洲黑熊四川亚种(ursus thibetanus mupinensis) 线粒体 12S RRNA 16S RRNA 序列分析
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四川黑熊RPS16亚基基因的克隆与序列分析 被引量:3
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作者 王婷 侯怡铃 +4 位作者 丁祥 孙冰 苏秀兰 李俊 侯万儒 《西华师范大学学报(自然科学版)》 2012年第4期350-355,共6页
根据已报道的部分哺乳动物RPS16基因的相关信息设计引物,运用RT-PCR技术,从四川黑熊的肌肉组织总RNA中对核糖体蛋白亚基RPS16基因的表达序列进行克隆、测序和分析.结果表明:四川黑熊RPS16基因的表达序列全长为481bp,开放阅读框(ORF)为44... 根据已报道的部分哺乳动物RPS16基因的相关信息设计引物,运用RT-PCR技术,从四川黑熊的肌肉组织总RNA中对核糖体蛋白亚基RPS16基因的表达序列进行克隆、测序和分析.结果表明:四川黑熊RPS16基因的表达序列全长为481bp,开放阅读框(ORF)为441bp,编码146个氨基酸,相对分子质量为16.4KDa,pI为10.81.拓扑预测显示含有6个类型的功能位点:1个依赖cAMP和cGMP的蛋白激酶磷酸化位点,3个蛋白激酶C磷酸化位点,1个酪蛋白激酶II磷酸化位点,2个N-糖基化位点,1个乙酰化位点及1个核糖体蛋白S9信号位点.进一步分析发现,四川黑熊RPS16基因与已报道的大熊猫、牛、野猪、人、小家鼠和褐家鼠等6个哺乳动物物种的表达序列及其编码的氨基酸序列有很高的相似性,说明RPS16基因具有高度的遗传稳定性和功能一致性. 展开更多
关键词 四川黑熊(ursus thibetanus mupinensis) RT—PCR RPS16 克隆 序列分析
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SEPARATING BROWN AND BLACK BEARS IN NORTHEAST CHINA USING TOOTH MEASUREMENTS
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作者 姜兆文 郑红 +1 位作者 王玉玺 张淑云 《Journal of Northeast Forestry University》 SCIE CAS CSCD 1995年第2期58-60,共3页
An expeditious method for ascertaining species between black bear (Selenarctos thibetanus G. cuiver) and brown bear (Ursus arctos Linnaeus) was developed using tooth measurements from previously identified specimens. ... An expeditious method for ascertaining species between black bear (Selenarctos thibetanus G. cuiver) and brown bear (Ursus arctos Linnaeus) was developed using tooth measurements from previously identified specimens. The measurement and analysis on 18 tooth measurement indexes (i.e. T1. Length of Pm1-Pm4, T2. L. of M1-M3, T3. M1L., T4. M1 width, T5. M2L., T6. M2W., T7.M3L., T8. M3W., T9. L. of C1 alveolus, T10. W. of C1 alveolus, T11. L. of C1-M2, T12. L. of Pm4 M2,T13.M1L., T14. M1W., T15. M2L., T16. M2W., T17.L. of C1 alveolus, T18. W. of C1 alveolus) of 59 skulls (black bears 25, brown bears 34) indicates that there are significant or the most significant difference between two species in every indexes, however T1, T9, T10, T17 and T18 are not suitable for species discriminator because of their high percent of overlap. The efficient species discriminators and standards (mm) are as follows T2(63.0), T3(22.0), T4(10.2), T5(23.8), T6(13.6), T7(18.0), T8(13.5), T11(110.0),T12(66.0), T13(20. 1), T14(15.8), T15(33.0) and T16(17.3). Those who are less than or equal to standard are classified to black bear, the others are brown bear. The method is very convenient and useful with high reliability. Their lowest accuracy percent are 95.0%, most of them over 97%. Different indexes will be chosen refer to the degree of specimen destroyed. 展开更多
关键词 BLACK BEAR (Selenarctos thibetanus) BROWN BEAR (ursus arctos) TOOTH measurement SEPARATING
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Resident Perceptions of Black Bear Management in South Central Kentucky
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作者 Hung-Ling Stella Liu Michael J. Bradley +1 位作者 I-Chun Nicky Wu James N. Maples 《Natural Resources》 2019年第7期271-283,共13页
The growth and sprawl of the American black bear (Ursus americanus) population have been observed and monitored in the past two decades, especially in south-central Kentucky. To better manage human-black bear interact... The growth and sprawl of the American black bear (Ursus americanus) population have been observed and monitored in the past two decades, especially in south-central Kentucky. To better manage human-black bear interaction and develop informed policy and best practices, the current study sought to 1) understand south-central Kentucky residents’ current knowledge of and previous experience with black bears, 2) investigate residents’ attitudes toward black bears and regulated hunting in wildlife management;and 3) identify residents’ level of acceptance of various black bear related management actions in south-central Kentucky. From February to April 2017, residents of London and Stearns districts (southeastern regions) of the Daniel Boone National Forest, a black bear habitat, were invited to participate in this study. The results from 139 completed surveys showed that residents lacked sufficient knowledge about black bear populations in the region and received minimal information regarding black bear management efforts. In comparison to education efforts and relocation, regulated black bear hunting could be a cost-effective option to promote responsible use of wildlife resources and black bear population control. This study provided lessons and recommendations for black bear management in south-central Kentucky and may be of utilization for any other wildlife conservation areas. 展开更多
关键词 Black BEAR (ursus AMERICANUS ) WILDLIFE MANAGEMENT National Forest WILDLIFE Tourism Interpretation Environmental Education KENTUCKY Hunting WILDLIFE RESOURCES Natural RESOURCES
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Climate Change and Its Impact on Brown Bear Distribution in Iran
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作者 Sahar Roshan Ara Sohrab Ashrafi +3 位作者 Roghayeh Garmaeepour Mohammad Zarrintab Nariman Askaripour Sorour Esfandeh 《Journal of Zoological Research》 2022年第1期1-11,共11页
Climate change is one of the threats in the recent century,affecting biodiversity directly and indirectly.Modeling the patterns of species distribution is one of useful tools for predicting the impacts of climate chan... Climate change is one of the threats in the recent century,affecting biodiversity directly and indirectly.Modeling the patterns of species distribution is one of useful tools for predicting the impacts of climate change on endangered species.Brown bear(Ursus arctos)plays an important role as a focal species in mountainous ecosystems.This study was aims to investigate the effects of future climate changes on the distribution of this species using an ensemble modeling method in R-software.For this purpose five algorithms including MAXENT,RF,MARS,GAM,GLM and BRT were used to predict the distribution of the species in the present climatic conditions as well as in the 2050s and 2070s.The results showed that temperature and precipitation were two main factors in the distribution of brown bears in Iran.Investigating the distribution of the brown bear in the future showed that suitability of its habitat will decrease in the western and central parts and increase in the northern parts.So a shift toward higher altitude will be expected for brown bear in the future.Therefore,in this condition it is imperative to upgrade the extent of protected areas for better conservation of brown bear. 展开更多
关键词 Iran ursus arctos Species distribution modeling R software Predicting
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Genetic analysis for geographic isolation comparison of brown bears living in the periphery of the Western Carpathians Mountains with bears living in other areas
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作者 Jan Graban Jana Kiskova +1 位作者 Pavol Pepich Robin Rigg 《Open Journal of Genetics》 2013年第3期174-182,共9页
Populations of the European brown bear (Ursus arctos L.) differ substantially in size, degree of geographic isolation and level of genetic diversity. Present patterns result from phylogeographic processes and profound... Populations of the European brown bear (Ursus arctos L.) differ substantially in size, degree of geographic isolation and level of genetic diversity. Present patterns result from phylogeographic processes and profound human intervention. We assessed the genetic variability of a subpopulation of brown bears near the periphery of their range in the Western Carpathian Mountains and compared their genetic properties with those of bears in the core of the same population and elsewhere. Samples were collected non-invasively in 2007-2008 and2010 in Strázovské Vrchy Protected Landscape Area (PLA) in Slovakia (included in the NATURA 2000 networking programme). Seven polymorphic microsatellite loci (UaMU26, UaMU64, G10B, G1D, G10L, UaMU50 and UaMU51) were amplified using a nested PCR in order to assess the following parameters: variability, allelic combinations, heterozygosity, number of alleles and inbreeding coefficient. Sufficient brown bear DNA for analysis was obtained from 57 out of 140 samples (41%), among which 45 different genotypes were identified. Loci had a mean of 2.71 ± 0.76 alleles. Average observed heterozygosity was 0.59. The inbreeding coefficient was negative for all but one of the analysed loci (2007-2008). In the year 2010 was negative three of seven loci. These results imply that gene flow with other parts of the population has been maintained in the reduced level and the isolation level of bears in the study area was not so low. Nevertheless, the genetic variability of bears in Strázovské Vrchy PLA was lower than that reported from other localities in the Carpathian Mountains. The results are discussed in the context of behavioural ecology and conservation genetics. 展开更多
关键词 Carpathian Mountains European Brown Bear ursus arctos L. Genetic Diversity Microsatellite Markers Non-Invasive Sampling
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微卫星复杂结构对分型的影响:以熊UamD116和UamB1为例 被引量:2
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作者 张小芳 杨淑慧 +2 位作者 马跃 徐艳春 宋伟杰 《现代生物医学进展》 CAS 2012年第20期3812-3816,共5页
目的:微卫星是基因组上的短串联重复序列,具有高度多态性,表现为核心序列中重复单位的重复次数的变化,这种变化造成不同等位基因核心序列的长度不同。因此,其基因型主要依靠PCR扩增片段长度来判定。在各类研究中,人们更倾向于使用4碱基... 目的:微卫星是基因组上的短串联重复序列,具有高度多态性,表现为核心序列中重复单位的重复次数的变化,这种变化造成不同等位基因核心序列的长度不同。因此,其基因型主要依靠PCR扩增片段长度来判定。在各类研究中,人们更倾向于使用4碱基重复的微卫星以减少2碱基微卫星的stutter等问题的影响。但是4碱基微卫星核心序列结构复杂时,就会对分型的正确性产生影响,从而影响到下游分析的正确性。在很多野生动物的研究中,这一问题常常被忽略。本文以亚洲黑熊(Ursus thibetanus)的2个四碱基微卫星位点UamD116和UamB1为例,揭示内部结构对分型的影响。方法:我们选用96份亚洲黑熊样品(包括血液、肌肉组织和毛发等样品)进行微卫星分型研究,通过荧光标记的PCR扩增和毛细管电泳分型,比较了基于扩增片段长度的分型和基于序列核心结构的分型效果的差异。结果:UamD116核心序列结构除了含有多种不同的重复单位外,还在重复单位之间有碱基插入,出现单碱基T、二碱基TC和三碱基AAG插入;并在一类等位基因下游侧翼序列有1个GA缺失。基于序列结构的分型中可以将不同的等位基因分开,而在基于片段长度的分型中,容易将不同的等位基因合并为1个等位基因。在位点UamB1共发现两种类型的等位基因,在一类等位基因中出现一个3bp的插入,使等位基因之间的差异不再是4bp,而是1bp。在仅依据片段长度分型时,相差1bp的等位基因被认定为1个。此外,还有不同等位基因核心序列不同,但是二者长度完全一致。依据片段长度分型共发现8个等位基因,而经过序列分型确定的等位基因数为12个,相应地基因频率及其他遗传学参数都发生相应的改变。结论:对于核心序列结构复杂的微卫星必须通过等位基因测序来矫正片段长度分型的结果,才能得到可靠的群体遗传学结论。 展开更多
关键词 微卫星 分型 等位基因结构 亚洲黑熊 ursus thibetanus
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气候变化对喜马拉雅中部甘达基河流域濒危物种的影响预测
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作者 Raju RAI 张镱锂 +4 位作者 刘林山 Paras Bikram SINGH Basanta PAUDEL Bipin Kumar ACHARYA Narendra Raj KHANAL 《Journal of Resources and Ecology》 CSCD 2022年第2期173-185,共13页
甘达基河流域位于喜马拉雅山脉中部,是众多濒危野生物种的重要栖息地。然而气候变化使该流域的生态环境变得愈发脆弱。本研究利用最大熵物种分布模型(Max Ent)评估气候变化对喜马拉雅黑熊(Ursus thibetanus laniger)和印度花豹(Panthera... 甘达基河流域位于喜马拉雅山脉中部,是众多濒危野生物种的重要栖息地。然而气候变化使该流域的生态环境变得愈发脆弱。本研究利用最大熵物种分布模型(Max Ent)评估气候变化对喜马拉雅黑熊(Ursus thibetanus laniger)和印度花豹(Panthera pardus fusca)等濒危物种空间分布变化的潜在影响。研究基于物种出没地点、生物气候和地形(海拔、坡度和坡向)等数据拟合模拟并预测物种在目前与未来的潜在分布情况。研究结果显示,目前喜马拉雅黑熊的高度适宜区面积约为1642 km^(2),占流域面积的5.01%,预计至2050年,其高度适宜区面积在甘达基河流域内将会增加约51 km^(2);印度花豹的高度适宜区面积约为3999 km^(2),占流域面积的12.19%,预计至2050年可能会增加到4806 km^(2)。喜马拉雅黑熊的栖息地面积可能会在研究区域的东部(伯塞里、塔托潘尼和班塞以北)增加,而在东部(颂当、切坎帕)、西部(布尔提邦和波邦)和北部(桑波切、玛南、切坎帕)减少;印度花豹的栖息地面积则将在研究区域东南部(班塞)、西部部分地区(赫里乔尔和桑迪哈克北部)和西北部(桑波切)增加,而在研究区域的东部、南部和其他西部地区减少。研究同时指出,海拔、Bio 15(季节性降水变化)和Bio 16(最湿润季度降水量)等环境因素对喜马拉雅黑熊的栖息地变化影响较大,而Bio 13(最湿润月降水量)和Bio 15(季节性降水变化)对印度花豹的栖息地变化影响较大。总之,这两个物种的栖息地在不同海拔下均会受到气候变化的影响,需要加强对该区域内濒危物种的保护力度。 展开更多
关键词 气候变化 物种栖息地变化 喜马拉雅黑熊(ursus thibetanus laniger) 印度花豹(Panthera pardus fusca) 甘达基河流域
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Using occupancy models to determine mammalian responses to landscape changes
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作者 Jeremy M.NICHOLSON Frank T.VAN MANEN 《Integrative Zoology》 SCIE CSCD 2009年第2期232-239,共8页
Determining impacts of anthropogenic landscape changes on wildlife populations is difficult.Besides the challengesDetermining impacts of anthropogenic landscape changes on wildlife populations is difficult.Besides the... Determining impacts of anthropogenic landscape changes on wildlife populations is difficult.Besides the challengesDetermining impacts of anthropogenic landscape changes on wildlife populations is difficult.Besides the challenges of designing field studies to document conditions before and after landscape changes occur,assessment of popula-of designing field studies to document conditions before and after landscape changes occur,assessment of popula-tion responses(e.g.changes in population density)often provide poor inference because of sampling limitations.tion responses(e.g.changes in population density)often provide poor inference because of sampling limitations.Estimation of occupancy,however,only requires data on detection or non-detection of a species and might provideEstimation of occupancy,however,only requires data on detection or non-detection of a species and might provide better inference.To demonstrate the utility of occupancy models,we used data from an American black bear(Ursusbetter inference.To demonstrate the utility of occupancy models,we used data from an American black bear(Ursus americanus Pallas)population in North Carolina,USA to test our research hypothesis that documented declines inamericanus Pallas)population in North Carolina,USA to test our research hypothesis that documented declines in site occupancy of black bears would be greater near a new four-lane highway.We used multi-season occupancysite occupancy of black bears would be greater near a new four-lane highway.We used multi-season occupancy models to estimate site occupancy based on bear visitation to survey sites before and after completion of the newmodels to estimate site occupancy based on bear visitation to survey sites before and after completion of the new highway and as a function of distance to the highway.Site occupancy declined from 0.81 to 0.35 between the twohighway and as a function of distance to the highway.Site occupancy declined from 0.81 to 0.35 between the two study phases,but was not a function of distance to the highway.Therefore,the impact of the new highway onstudy phases,but was not a function of distance to the highway.Therefore,the impact of the new highway on occupancy extended to the entire study area.Our case study demonstrates that occupancy models can provideoccupancy extended to the entire study area.Our case study demonstrates that occupancy models can provide powerful inference regarding the potential impacts of landscape changes on species occupancy.As urban areas andpowerful inference regarding the potential impacts of landscape changes on species occupancy.As urban areas and transportation infrastructure are rapidly expanding in developing regions of the world,the need to determine howtransportation infrastructure are rapidly expanding in developing regions of the world,the need to determine how these changes affect mammal populations and how they might be mitigated increases accordingly.Because fieldthese changes affect mammal populations and how they might be mitigated increases accordingly.Because field sampling for occupancy models only requires detection data,surveys can be conducted for extensive geographicsampling for occupancy models only requires detection data,surveys can be conducted for extensive geographic areas,thus making these surveys particularly applicable to studies of large mammals.areas,thus making these surveys particularly applicable to studies of large mammals. 展开更多
关键词 landscape changes mammalian responses occupancy models transportation infrastructure ursus americanus.
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