Subgroups of peripheral immune effector cells in cervical cancer patients are more sensitive to radiation therapy than chemotherapy

Background:CD8 positive T lymphocytes and natural killer(NK)cells in the peripheral blood of cervical cancer patients exhibit varying sensitivities to radiotherapy and chemotherapy.Methods:A total of 50 healthy peoples and 60 cervical cancer patients were recruited.The patients with cervical cancer were separated into two groups:radiation and chemotherapy,and blood sample were collected before and after treatment.Data on the proportion of CD8 positive T lymphocytes and NK cells were gathered for analytical evaluation.Results:Compared to healthy individuals,patients with cervical cancer exhibit a reduced proportion of CD8 positive T cells within their peripheral blood.And for patients with cervical cancer,radiation therapy has been found to be more effective than chemotherapy in increasing the proportion of CD8 positive T lymphocytes and NK cells.Conclusions:These results suggest that radiation therapy increases the levels of CD8 positive T lymphocytes and NK cells within the peripheral blood of patients with cervical cancer.The study hypothesis that the changes in the percentage of CD8 positive T lymphocytes may serve as a potential indicator for predicting treatment efficacy.

Knockdown of circular RNA (CircRNA)_001896 inhibits cervical cancer proliferation and stemness in vivo and in vitro

Objective:Previous studies indicated that aberrant circular RNA(circRNA)expression affects gene expression regulatory networks,leading to the aberrant activation of tumor pathways and promoting tumor cell growth.However,the expression,clinical significance,and effects on cell propagation,invasion,and dissemination of circRNA_001896 in cervical cancer(CC)tissues remain unclear.Methods:The Gene Expression Omnibus(GEO)datasets(GSE113696 and GSE102686)were used to examine differential circRNA expression in CC and adjacent tissues.The expression of circRNA_001896 was detected in 72 CC patients usingfluorescence quantitative PCR.Correlation analysis with clinical pathological features was performed through COX multivariate and univariate analysis.The effect of circRNA_001896 downregulation on CC cell propagation was examined using the cell counting kit-8(CCK-8)test,clonogenic,3D sphere formation,and in vivo tumorigenesis assays.Results:Intersection of the GSE113696 and GSE102686 datasets revealed an increased expression of four circRNAs,including circRNA_001896,in CC tissues.Fluorescence quantitative PCR confirmed circRNA_001896 as a circular RNA.High expression of circRNA_001896 was considerably associated with lymph node metastasis,International Federation of Gynecologists and Obstetricians(FIGO)stage,tumor diameter,and survival period in CC patients.Proportional hazards model(COX)univariate and multivariate analyses revealed that circRNA_001896 expressions are a distinct risk factor affecting CC patients’prognosis.Cellular functional experiments showed that downregulating circRNA_001896 substantially suppressed CC cell growth,colony formation,and 3D sphere-forming ability.In vivo,tumorigenesis analysis in nude mice demonstrated that downregulating circRNA_001896 remarkably reduced the in vivo proliferation capacity of CC cells.Conclusion:CircRNA_001896 is highly expressed in CC tissues and is substantially related to lymph node metastasis,FIGO stage,tumor size,and survival period in patients.Moreover,downregulating circRNA_001896 significantly inhibits both in vivo and in vitro propagation of CC cells.Therefore,circRNA_001896 might be used as a biomarker for targeted therapy in cervical cancer.

Relationship between Circulating Plasma Galectin-3 Levels and T-Cell Activation during Cervical Cancer Chemotherapy

Objective: Despite the existence of several therapeutic strategies, the management of cervical cancer remains challenging. Our region has very little data on the interaction between the immune system and the clinical response to chemotherapy. This work examines plasma levels of galectin-3 (Gal-3) and percentages of activated T cells in patients with cervical cancer treated with chemotherapy and investigates if there is a relationship between the rates of these two elements. Methods: We compared data from 37 patients with cervical cancer undergoing chemotherapy and 42 controls with normal cervical cytology. Plasma Gal-3 concentrations were assessed by ELISA and expression of activation markers by T cells (CD69 and HLA-DR) was assessed by flow cytometry at three different time points during chemotherapy. Results: Our results showed that patients had a significantly higher concentration of Gal-3 compared to controls (4.025 vs. 1.340, p 0.001), similarly, they had a significantly high percentage of activated lymphocytes (2.610 vs. 0.731;p 0.0001). According to the response to treatment, patients with no response to treatment had a lower concentration of circulating Gal-3 but had approximately the same percentage of activated CD4 and CD8 lymphocytes as patients with a partial or total response. In addition, we found a positive correlation between the Gal-3 level and CD4 T cells expressing the activation marker CD69 (p 0.05;rho = 0.44). Conclusion: In conclusion, our results show that there would be a relationship between circulating galectin-3 and the percentage of peripheral CD4+CD69+ cells in cervical cancer.

<i>FAS</i>-670 Gene Promoter Region Polymorphism in Cervical Intraepithelial Neoplasia Grade 3 (CIN 3) and Invasive Cervical Cancer

The human papillomavirus (HPV) is the etiologic agent of genital cancer, but alone it is incapable of inducing oncogenesis. Rather, progression to invasive lesions is associated with host immunity and interference in the process of cellular apoptosis. Among the several genes involved in cell death, theFAS gene appears to be an important factor. The aim of this study was to evaluate whether there is an association between polymorphisms of the FAS-670 gene promoter region and preinvasive and invasive lesions of the cervix. The FAS gene was evaluated for the presence of polymorphisms using polymerase chain reaction and restriction fragment length polymorphism techniques in 225 blood samples for the control group, as well as cervical tissue from patients with cervical intraepithelial neoplasia grade 3 (CIN 3;75 cases) or invasive carcinoma (214 cases). The AG genotype of the FAS-670 gene promoter region was significantly more frequent in women with CIN 3, with an estimated risk of three times (OR = 3.0). No difference, however, was observed in the control group and women with cervical cancer. In women with cancer, the genotypes were similar in the different histological types and degree of tumor differentiation. Assessing allelic distribution (A or G), we observed no difference in frequency of genotypes in studied groups. These data suggest that polymorphism of the promoter region of the FAS-670 gene is associated with increased risk of CIN 3, but not for invasive cancer of the cervix.

Quantitative Detection of Screening for Cervical Lesions with ThinPrep Cytology Test

OBJECTIVE To investigate the available parameters in gynecological screening for cervical lesions by liquid-based cytology technology （ThinPrep Cytology Test, TCT） and The Bethesda System （TBS）, also with computer image analysis. METHODS With application of the image analysis system, all grades of cervical lesion cells were detected quantitatively and sorted in atypical squamous cells of undetermined significance （ASCUS）, atypical squamous cells-cannot exclude HSIL （ASC-H）, low-grade squamous intraepithelial lesion （LSIL）, high-grade squamous intraepithelial lesion （HSIL） and cervical squamous cell carcinoma （SCC） with the mean optical density （MOD）, average grey （AG）, positive units （PU）, and nucleus to cytoplasmic ratio （N： C）. Differences between each group of cells were compared and analyzed statistically. RESULTS Apart from four stereologic parameters in LSIL and HSIL groups there were no differences among them, in the other groups, there was statistically significant in differences between MOD, AG and PU values. Differences between them in the ratio of nucleus to cytoplasm were highly statistically significant. CONCLUSION Stereological indexes may serve as a screening tool for cervical lesions. The image analysis system is expected to become a new means of cytological assisted diagnosis.

宫颈癌中单核细胞对Th17细胞分化的影响

目的 检测宫颈癌病人外周血中Th17细胞的比例及相关细胞因子白细胞介素(IL)-1β、IL-6的表达水平,探讨宫颈癌外周血中单核细胞对Th17细胞分化的影响及机制。方法 2019年6月至2020年12月在潍坊市人民医院妇科收治的宫颈癌病人(宫颈癌组)39例,对照组(30例)为健康志愿者及因良性疾病在潍坊市人民医院妇科就诊的病人,流式细胞仪检测宫颈癌组、对照组外周血中Th17细胞的比例;实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测宫颈癌病人、对照组外周血中IL-1β、IL-6的表达水平;分离健康志愿者外周血中的初始CD4^(+)T细胞,分别与对照组、宫颈癌组外周血中分离出的单核细胞进行共培养,并分别加入anti-IL-1β、anti-IL-6中和抗体,流式细胞仪检测Th17细胞的比例。结果 宫颈癌组外周血中,Th17细胞比例(3.5±1.4)%、IL-1β 0.457(0.078,1.193)、IL-6 0.094(0.043,0.272)的表达水平明显高于对照组(2.3±1.6)%、0.279(0.017,0.686)、0.038(0.019,0.112)。初始CD4^(+)T细胞与宫颈癌组外周血中的单核细胞共培养,Th17细胞分化的比例(1.77±0.61)%显著高于对照组(0.90±0.39)%;与对照组相比(1.82±0.47)%,加入中和抗体后,Th17细胞的分化比例均下降,且加入anti-IL-1β组(0.76±0.23)%与加入anti-IL-6(1.06±0.34)%组比,前者Th17细胞分化比例下降更为明显。结论 宫颈癌病人外周血中的单核细胞更能促进Th17细胞的分化。在Th17细胞分化的过程中,IL-1β、IL6起到重要作用,且IL-1β在促进Th17细胞分化的过程中诱导作用更强。

宫颈癌患者血清Stathmin、Sox2及VEGF mRNA表达与淋巴结转移的相关性

目的探讨宫颈癌患者血清Stathmin、Sox2及血管内皮生长因子(VEGF)mRNA表达与淋巴结转移的相关性。方法选取2017年1月—2020年2月收治的宫颈癌80例作为A组,另选取同期宫颈上皮内瘤样病变60例作为B组以及行子宫全切术的其他子宫良性病变60例作为C组。采用PT-PCR检测3组血清VEGF、Sox2、Stathmin mRNA表达情况;采用Pearson相关系数分析血清VEGF、Sox2、Stathmin mRNA表达与临床特征的相关性;采用多因素Logistic回归分析探讨宫颈癌患者淋巴结转移危险因素。结果A组血清VEGF、Sox2、Stathmin mRNA表达水平均高于B组和C组,且B组高于C组(P<0.05)。淋巴结转移宫颈癌患者血清VEGF、Sox2、Stathmin mRNA表达水平均高于无淋巴结转移患者(P<0.05)。血清VEGF、Sox2、Stathmin mRNA表达均与淋巴结转移、分化程度、国际妇产科学联合会(FIGO)分期呈显著正相关(P<0.01)。FIGO分期、分化程度、肿瘤直径及血清VEGF、Sox2、Stathmin mRNA表达为宫颈癌患者淋巴结转移的独立危险因素(P<0.01)。结论宫颈癌患者血清VEGF、Sox2和Stathmin mRNA表达上调,其表达水平与淋巴结转移密切相关。

妊娠期宫颈癌临床误诊分析

目的探讨妊娠期宫颈癌临床误诊的原因及防范措施。方法回顾性分析2020年1月—2021年12月收治的曾有误诊经过的妊娠期宫颈癌3例临床资料。结果3例均因妊娠期阴道出血、腹痛或阴道分泌物异常就诊。1例考虑为先兆早产,剖宫产术后行MRI检查发现宫颈明显不规则增厚伴异常信号,经增厚组织病理检查确诊为宫颈低分化鳞状细胞癌(鳞癌);1例考虑为先兆流产,妇科检查发现宫颈有一赘生物,经宫颈活检确诊为宫颈中分化鳞癌;1例考虑为前置胎盘,剖宫产术后宫颈内口触及胎盘样组织,腹部超声示宫颈与宫体结合部占位性病变,病理活检示宫颈鳞癌。误诊时间2 d~4个月。3例确诊后予宫颈癌根治术联合术后放疗,随访半年情况良好。结论妊娠期宫颈癌临床少见,临床对以妊娠期出现不规则阴道出血、腹痛及分泌物异常增多就诊者提高警惕,以减少误诊。

宫颈癌保留神经手术后排尿功能评价

目的探讨宫颈癌保留神经的广泛性子宫切除术(nerve sparing radical hysterectomy,NSRH)治疗宫颈癌的效果及对排尿功能的影响。方法纳入2019年5月至2022年5月于首都医科大学附属北京妇产医院就诊并诊断为宫颈癌的患者55例,国际妇产科联盟分期分布在ⅠB至ⅡA2。其中28例患者接受了NSRH(NSRH组),27例患者接受了广泛性全子宫切除术(radical hysterectomy,RH)(RH组)。比较2组患者手术相关指标及术后排尿功能。结果NSRH组平均年龄为(51.0±7.9)岁,RH组平均年龄为(46.3±8.5)岁,年龄分布差异有统计学意义(P<0.05)。2组出血量差异无统计学意义(P>0.05)。2组患者手术切缘均为阴性。术后病理提示阴道长度、淋巴结转移、间质浸润深度、淋巴脉管浸润比较差异均无统计学意义(P>0.05)。在术后膀胱功能方面,NSRH组残余尿较RH组多(P<0.05),但NSRH组拔尿管的时间较RH组明显缩短(P<0.05)。结论NSRH能够在确保手术范围的同时,减轻对术后排尿功能的影响,缩短术后恢复时间。

宫颈癌肉瘤一例并文献复习

宫颈癌肉瘤(cervical carcinosarcoma)是一种由恶性的上皮组织和间质成分组成的恶性肿瘤,患者通常以不规则阴道出血、流液就诊,其病因及发病机制尚不明确,临床上十分罕见,因此容易造成误诊和漏诊。现报告1例42岁宫颈癌肉瘤的病例资料,患者因接触性出血伴下腹痛收入院,盆腔磁共振成像示宫颈部体积明显增大,初步诊断为宫颈恶性肿瘤,遂行根治性全子宫切除+盆腔淋巴结切除+大网膜切除术,术后病理学明确诊断为宫颈癌肉瘤Ⅱb期。截至2024年3月2日,患者已行3次化疗,暂未发现复发及转移。宫颈癌肉瘤恶性程度高,临床表现无特异性,确诊主要依靠病理学检查和免疫组织化学检查,因此早识别、早治疗对改善患者的预后至关重要。

循环肿瘤细胞上细胞程序性死亡配体1表达对宫颈癌同步放化疗预后的评估价值

目的探讨循环肿瘤细胞(CTC)上细胞程序性死亡配体1(PD-L1)表达对宫颈癌同步放化疗预后的评估价值。方法回顾性选取2021年6月至2023年12月入临汾市人民医院的60例宫颈癌患者,均经病理学确诊,CTC PD-L1经CytoSorter CTC系统测定。对宫颈癌病理特征与PD-L1^(+)CTC之间的相关性予以分析,并比较PD-L1^(+)CTC和PD-L1-CTC宫颈癌患者同步放化疗前后的无进展生存期(PFS),评估宫颈癌放化疗后PD-L1^(+)CTC数量对预后的评估价值。结果同步放化疗前后外周血CTC检出率及数量比较,差异无统计学意义(P>0.05)。较同步放化疗前,宫颈癌患者同步放化疗后PD-L1^(+)CTC检出率更低(38.33%vs.56.67%),PD-L1^(+)CTC检出数量更少[(0.77±1.20)个vs.(1.27±1.51)个],差异均有统计学意义(P<0.05)。同步放化疗前,宫颈癌患者PD-L1^(+)CTC与肿瘤直径、FIGO分期、分化程度及淋巴结转移联系紧密(P<0.05)。同步放化疗后,宫颈癌患者PD-L1^(+)CTC表达水平与肿瘤直径、FIGO分期、分化程度及淋巴结转移密切相关(P<0.05)。PD-L1^(+)CTC患者的PFS短于PD-L1-CTC患者,差异有统计学意义(P<0.05)。Cox回归分析显示,局部晚期宫颈癌患者同步放化疗前后的CTC PD-L1表达水平均可作为PFS预后因素。结论宫颈癌放同步放化疗前后测定的CTC PD-L1表达水平可作为预后评估的辅助指标。

PD⁃1抑制剂联合全身化疗治疗复发转移性宫颈癌的临床效果观察

目的探讨程序性细胞死亡受体-1(PD-1)抑制剂(卡瑞利珠单抗)免疫治疗联合全身化疗治疗复发转移性宫颈癌的临床效果。方法选择2019年2月—2021年6月定州市人民医院收治的复发转移性宫颈癌64例,依据随机数字表法分为研究组和对照组各32例。对照组给予紫杉醇联合顺铂全身化疗方案,研究组给予全身化疗方案+PD-1卡瑞利珠单抗免疫治疗。比较2组临床疗效,分析治疗前及治疗3个周期后鳞状细胞癌抗原(SCC)、外周血淋巴细胞/单核细胞(LMR)及血小板/淋巴细胞(PLR)指标水平及Kamofsky评分变化,并观察治疗期间毒性作用发生情况及随访期间患者总生存期。结果研究组总有效率、疾病控制率分别为93.75%(30/32)、96.88%(31/32),高于对照组的68.75%(22/32)、75.00%(24/32),差异有统计学意义(P<0.05)。治疗3个周期后,2组血清SCC、PLR水平较治疗前降低,LMR较治疗前升高,且研究组改善程度优于对照组(P<0.05)。治疗后,2组Kamofsky评分均较治疗前升高,且研究组高于对照组(P<0.05)。治疗后研究组1、2年生存率及总生存期高于或长于对照组(P<0.05)。2组毒性作用多数为1~2级。研究组血小板下降和转氨酶升高比例分别为37.50%(12/32)和28.12%(9/32),高于对照组的18.75%(6/32)和9.38%(3/32),差异有统计学意义(P<0.05);2组贫血、白细胞下降、恶心、腹泻、乏力等毒性作用发生率比较差异无统计学意义(P>0.05);研究组中发生反应性毛细血管增生症、甲状腺功能减退、皮疹、带状疱疹、过敏等经对症处理后症状消失。结论PD-1抑制剂联合全身化疗治疗复发转移性宫颈癌提高了临床效果、生存质量及生存率,延长生存期,改善了机体的炎症免疫反应状态,毒性作用较少,患者耐受性好。

m6A修饰与妇科恶性肿瘤相关性的研究进展

N6-甲基腺苷(m6A)修饰是一种常见的表观遗传学修饰,动态调控RNA代谢的整个过程。m6A包括甲基转移酶、去甲基酶及m6A结合蛋白。m6A的甲基化修饰通过调节RNA的衰变、运输、剪切、翻译来调节细胞的增殖和分化,m6A与妇科恶性肿瘤的发生发展有关。笔者就近年来m6A甲基化修饰在宫颈癌、子宫内膜癌和卵巢癌中的相关研究进展进行回顾,分析m6A通过作用于RNA的修饰调节妇科恶性肿瘤发生发展的机制,为妇科恶性肿瘤的治疗、改善预后提供新的方向。

铜死亡在妇科恶性肿瘤中的研究进展

铜离子作为重要的辅酶,参与了广泛的代谢过程,包括有氧呼吸、肽酰胺化、铁转运、超氧化物歧化和细胞外基质的生物合成。铜稳态是维持细胞活性的重要机制。铜死亡是由铜离子积累引发的细胞死亡形式,其特点是铜稳态失衡,细胞内铜积累,选择性地扰乱三羧酸循环,使脂酰化线粒体酶聚集和铁硫蛋白丢失,引起线粒体蛋白质毒性应激,细胞膜裂解,最终导致细胞死亡。妇科恶性肿瘤是引起女性癌症死亡的重要原因。研究发现,铜死亡与妇科恶性肿瘤的发生、发展、预后及耐药密切相关。一些铜死亡调节剂可通过抑制血管生成、调节癌细胞的耐药性,抑制肿瘤转移和复发,因此铜死亡是治疗妇科恶性肿瘤有价值的研究方向。

The delay, symptoms, and survival of Ivorian adolescent girls and young adults with uterine cervical cancer

Objective:To generate data on the nature and duration of cervical cancer symptoms,risk factors for delayed consultation,and diagnosis of adolescent and young adult groups in Cote d'Ivoire.Methods:This is a hospital-based cross-sectional study,conducted from July 2012 to May 2018,at the Department of Gynecology of the Yopougon teaching hospital in Cote d’Ivoire.The inclusion criteria were those who were under 40 years of age at the time of diagnosis with oral and written consent.Those whose information was more than 10%insufficient for the standards were excluded from the study.The survey files standardized anonymous pre-established data collected using medical records followed by phone calls.Results:The average age of the participants was 34±4.95 years with a minimum of 21 years.The mean parity per woman was 3.49±3.54.34.9%were HIV positive.The median patient delay was 122 days with a long delay in 84.62%of patients.The median time to total diagnosis was 209 days with a longer total delay in diagnosis of 87.18%.41%of patients honored their treatment,68.75%of which had received surgery,and 31.25%of which had received chemotherapy.The 5-year survival rate was 65%.Conclusion:The Ivorian health system must focus on primary and secondary prevention,the only guarantee to decrease the morbidity and mortality indicators linked to cervical cancer.

T_(2)mapping成像纹理分析鉴别宫颈癌病理分型和鳞癌病理分级

目的:探讨T_(2)mapping成像纹理分析鉴别宫颈癌病理分型和鳞癌病理分级的价值。方法:回顾性分析54例宫颈鳞癌(高级别37例、中-低级别17例)、19例腺癌患者的资料,术前均行T_(2)mapping序列在内的3.0TMRI检查。两名观察者分别独立提取T_(2)mapping全肿瘤纹理参数。比较鳞癌与腺癌之间及高级别与中-低级别鳞癌之间参数的差异性,采用Logistic回归构建联合参数。采用受试者工作特征(Receiver operating characteristic,ROC)曲线评估单一纹理参数及联合参数鉴别宫颈癌病理分型及鳞癌病理分级的效能。采用DeLong检验对比分析单一纹理参数和联合参数曲线下面积(Area under the curve,AUC)的差异。结果:从每例患者肿瘤中提取88个纹理参数,最终筛选出3个参数用于鉴别病理分型,8个参数用于鉴别鳞癌病理分级(P均<0.05)。鉴别宫颈癌病理分型及鳞癌病理分级的联合参数的AUC分别为0.963、0.966。联合参数的AUC较单一参数的AUC显著提高。结论:基于T_(2)mapping成像的纹理分析对于宫颈癌病理分型和鳞癌病理分级的鉴别具有一定的价值。

妊娠合并宫颈大细胞神经内分泌癌和低钠血症一例

回顾分析1例妊娠合并宫颈大细胞神经内分泌癌ⅢC1(r)期剖宫产术后行静脉化疗发生低钠血症的患者,经放化疗治疗原发肿瘤,对症治疗纠正低钠血症达完全缓解后6个月,出现疾病复发,通过化疗联合靶向及免疫治疗,患者因出现分布性休克、电解质紊乱,患者家属放弃治疗,随访至2023年5月,患者死亡。宫颈大细胞神经内分泌癌是一种恶性程度高、预后差的恶性肿瘤,患者出现低钠血症可能与肿瘤的不良预后相关,临床上容易被化疗的不良反应所掩盖。临床医生应掌握低钠血症的临床表现,提高早期识别的能力,做到早诊断、早治疗,积极改善患者预后。

信迪利单抗联合常规化疗在复发转移宫颈癌治疗中疗效和安全性研究

目的探究程序性死亡受体1(PD-1)抑制剂信迪利单抗(达伯舒)联合常规化疗在复发转移宫颈癌治疗中短期疗效、长期疗效和安全性。方法筛选2019年3月至2020年8月河北北方学院附属第一医院符合纳入、排除标准的复发转移宫颈癌病人88例,采用中央随机系统分配法分为试验组(n=44)和对照组(n=44)。对照组执行常规化疗方案,试验组执行信迪利单抗联合常规化疗方案。参考实体肿瘤疗效评估标准(RECIST)1.1来评价并比较两组病人短期疗效。通过随访和记录无进展生存期(PFS)来评价并比较两组病人长期疗效。参考常见不良事件评价标准(CTCAE)5.0版来评价两组病人不良事件和安全性。结果短期疗效:试验组客观缓解率为47.73%,显著高于对照组18.18%(P=0.035);试验组持久临床受益率为86.36%,显著高于对照组的43.18%(P=0.048)。长期疗效:试验组中位PFS为7.22个月[95%CI:(4.38,14.04)],显著长于对照组的4.31个月[95%CI:(3.43,9.25)](P=0.032)。试验组中位总生存期(OS)为17.30个月[95%CI:(14.33,17.58)],死亡风险显著低于对照组的6.93个月[95%CI:(4.89,13.25)](P=0.039)。Cox回归分析结果显示肿瘤突变负荷(P=0.036)、化疗疗程(P=0.022)、组织学类型(P=0.018)和转移灶位点(P=0.035)是试验组治疗效果的独立预后因素。结论信迪利单抗联合常规化疗方案显著提高了复发转移宫颈癌病人的短、长期疗效,不良反应未明显增加,具有一定临床价值。

B7-H6在宫颈上皮内瘤变、宫颈癌组织中表达差异及与肿瘤浸润T淋巴细胞、预后的相关性

目的探究宫颈上皮内瘤变、宫颈癌组织中B7同源体6(B7-H6)表达差异与肿瘤浸润T淋巴细胞、预后相关性。方法选取2019年6月—2021年3月60例经病理检查确诊为宫颈癌的手术患者作为研究组,另选同期同年龄段60例宫颈上皮内瘤变作为对照组。比较2组组织B7-H6表达情况(H-score评分)及血清B7-H6水平,并比较研究组不同肿瘤浸润T淋巴细胞浸润程度患者H-score评分、血清B7-H6水平,分析研究组H-score评分、血清B7-H6水平与肿瘤浸润T淋巴细胞浸润程度的相关性。研究组术后随访1年,比较不同预后患者一般资料、H-score评分、血清B7-H6水平,探讨预后影响因素,并分析H-score评分和血清B7-H6水平预测患者预后的价值。结果研究组H-score评分和血清B7-H6水平高于对照组(P<0.01)。研究组肿瘤浸润T淋巴细胞浸润程度0级患者H-score评分、血清B7-H6水平高于1级、2级患者,且1级患者高于2级患者(P<0.05)。研究组H-score评分、血清B7-H6水平与肿瘤浸润T淋巴细胞浸润程度呈负相关(P<0.01)。多因素Logistic回归分析显示,肿瘤直径、国际妇产科协会(FIGO)分期、淋巴结转移、间质浸润深度、H-score评分、血清B7-H6水平均为宫颈癌手术患者预后不良的独立危险因素(P<0.01)。肿瘤直径、FIGO分期、淋巴结转移、间质浸润深度、H-score评分和血清B7-H6水平预测宫颈癌手术患者预后不良的曲线下面积(AUC)分别为0.736、0.726、0.714、0.712、0.842和0.840,H-score评分和血清B7-H6水平预测的AUC分别大于肿瘤直径、FIGO分期、淋巴结转移、间质浸润深度预测的AUC(P<0.05)。H-score评分和血清B7-H6水平在预测宫颈癌手术患者预后不良方面拥有良好的临床效用。结论宫颈癌组织B7-H6表达高于宫颈上皮内瘤变,且宫颈癌组织B7-H6表达、血清B7-H6水平与肿瘤浸润T淋巴细胞浸润程度呈负相关,也是宫颈癌手术患者预后不良的独立危险因素,在预测预后方面具有一定临床价值。

预测宫颈癌合并糖尿病患者术后切口感染风险的Nomogram模型建立

目的旨在构建预测宫颈癌合并糖尿病患者根治性子宫切除术术后切口感染风险的列线图模型,并进行模型评估。方法选取在本院接受根治性子宫切除术治疗的宫颈癌合并糖尿病患者267例作为研究对象,其中发生术后切口感染(感染组)97例,未发生术后切口感染(未感染组)170例。采用单因素和多因素Logistic回归筛选与术后切口感染相关的独立影响因素。采用R软件和相关程序包绘制列线图模型。结果与未感染组相比,感染组年龄≥55岁、体重指数(body mass index,BMI)≥25、引流管留置时间≥7 d、住院时间≥14 d、血清白蛋白<30 g/L的患者比例明显增加(P<0.05)。与未感染组相比,感染组血清白细胞介素6(interleukin-6,IL-6)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、C反应蛋白(C-reactive protein,CRP)和降钙素原(procalcitonin,PCT)水平显著升高(P<0.05)。多变量Logistic回归分析表明,年龄≥55岁、BMI≥25、引流管留置时间≥7 d、住院时间≥14 d,以及血清IL-6、TNF-α、CRP和PCT水平升高均是术后切口感染发生的独立危险因素(P<0.05)。用于评估宫颈癌合并糖尿病患者术后切口感染发生风险的列线图具有良好的预测准确性(C指数为0.947)、区分度(受试者工作特征曲线下面积为0.947)、一致性(Hosmer-Lemeshow拟合优度检验平均绝对误差为0.011)和临床效能。结论本研究基于围手术期特征构建了预测宫颈癌合并糖尿病患者术后切口感染的列线图模型,该模型可能有助于加强感染控制意识,为根治性子宫切除术后高危患者的管理提供参考。