Therapeutic Effect of Rhodopseudomonas palustris-Bioconverted Extract of Lycium barbarum on Rats with Lead Exposure

Lead is a ubiquitous pollutant and Pb pollution is a global public health problem.Lead has been reported to induce multiple adverse effects,including reproductive toxicity,neurotoxicity,carcinogenicity,nephrotoxicity,immunotoxicity,and hypertension[1].The traditional medical treatment available for Pb poisoning is chelation,which can save lives in individuals with very high blood Pb levels.The commonly used chelating agents include CaNa2EDTA and meso-2,3-dimercaptosuccinic acid.However,chelation therapy has strong short-term effects on the overall long-term management of Pb exposure.

Recent advancements in continuously scalable conversion-ratio switched-capacitor converter

Switched-capacitor(SC)DC-DC converter[1]is an impor-tant alternative to inductive DC-DC converter,in terms of removing the bulky power inductor.Hence,it is widely used in low-profile,low-power applications,such as the internet of things(IoT)sensor nodes and energy harvesting[2].Mean-while,considering that capacitor has a much higher energy density than inductor,high-power applications.

Energy-Efficient Implementation of BCD to Excess-3 Code Converter for Nano-Communication Using QCA Technology

Code converters are essential in digital nano communication;therefore,a low-complexity optimal QCA layout for a BCD to Excess-3 code converter has been proposed in this paper.A QCA clockphase-based design technique was adopted to investigate integration with other complicated circuits.Using a unique XOR gate,the recommended circuit’s cell complexity has been decreased.The findings produced using the QCADesigner-2.0.3,a reliable simulation tool,prove the effectiveness of the current structure over earlier designs by considering the number of cells deployed,the area occupied,and the latency as design metrics.In addition,the popular tool QCAPro was used to estimate the energy dissipation of the proposed design.The proposed technique reduces the occupied space by∼40%,improves cell complexity by∼20%,and reduces energy dissipation by∼1.8 times(atγ=1.5EK)compared to the current scalable designs.This paper also studied the suggested structure’s energy dissipation and compared it to existing works for a better performance evaluation.

Robust optical mode converter based on topological waveguide arrays

Optical mode converters are essential for enhancing the capacity of optical communication systems. However, fabrication errors restrict the further improvement of conventional mode converters. To address this challenge, we have designed an on-chip TE0–TE1mode converter based on topologically protected waveguide arrays. The simulation results demonstrate that the converter exhibits a mode coupling efficiency of 93.5% near 1550 nm and can tolerate a relative fabrication error of 30%. Our design approach can be extended to enhance the robustness for other integrated photonic devices, beneficial for future development of optical network systems.

Extracellular vesicles and angiotensin-converting enzyme 2 in COVID-19 disease

Extracellular vesicles(EVs)are membranous vesicular structures released from almost all eukaryotic cell types under different physiological or pathological conditions.Growing evidence demonstrates that EVs can serve as mediators of intercellular communication between donor and recipient cells or microorganism-infected and noninfected cells.Coronavirus disease 2019(COVID-19)disease is caused by infection of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)of host cells in the respiratory system and various extra-pulmonary tissue/organs,resulting in complications of multiple organ systems.As the cell surface receptor,angiotensin-converting enzyme 2(ACE2)mediates cellular entry of SARS-CoV-2 into the host cells in patients with COVID-19.Recent studies have found that ACE2 can be released with EVs,which have been shown to interfere with the entry of the virus into host cells and thus may be involved in COVID-19 pathophysiology.In addition,ACE2,neprilysin(NEP),and thimet oligopeptidase(TOP)are the key enzymes that regulate angiotensin metabolism by converting angiotensin II or angiotensin I to angiotensin 1-7,the latter of which has protective effects in counterbalancing the harmful effects of angiotensin II in COVID-19 disease.This review summarizes the recent research progress regarding EV-associated ACE2,NEP,and TOP and the perspectives of their potential involvement in the pathophysiology of COVID-19 disease.

ANovel Non-Isolated Cubic DC-DC Converter with High Voltage Gain for Renewable Energy Power Generation System

In recent years,switched inductor(SL)technology,switched capacitor(SC)technology,and switched inductor-capacitor(SL-SC)technology have been widely applied to optimize and improve DC-DC boost converters,which can effectively enhance voltage gain and reduce device stress.To address the issue of low output voltage in current renewable energy power generation systems,this study proposes a novel non-isolated cubic high-gain DC-DC converter based on the traditional quadratic DC-DC boost converter by incorporating a SC and a SL-SC unit.Firstly,the proposed converter’s details are elaborated,including its topology structure,operating mode,voltage gain,device stress,and power loss.Subsequently,a comparative analysis is conducted on the voltage gain and device stress between the proposed converter and other high-gain converters.Then,a closed-loop simulation system is constructed to obtain simulation waveforms of various devices and explore the dynamic performance.Finally,an experimental prototype is built,experimental waveforms are obtained,and the experimental dynamic performance and conversion efficiency are analyzed.The theoretical analysis’s correctness is verified through simulation and experimental results.The proposed converter has advantages such as high voltage gain,low device stress,high conversion efficiency,simple control,and wide input voltage range,achieving a good balance between voltage gain,device stress,and power loss.The proposed converter is well-suited for renewable energy systems and holds theoretical significance and practical value in renewable energy applications.It provides an effective solution to the issue of low output voltage in renewable energy power generation systems.

An Adaptive Control Strategy for Energy Storage Interface Converter Based on Analogous Virtual Synchronous Generator

In the DC microgrid,the lack of inertia and damping in power electronic converters results in poor stability of DC bus voltage and low inertia of the DC microgrid during fluctuations in load and photovoltaic power.To address this issue,the application of a virtual synchronous generator(VSG)in grid-connected inverters control is referenced and proposes a control strategy called the analogous virtual synchronous generator(AVSG)control strategy for the interface DC/DC converter of the battery in the microgrid.Besides,a flexible parameter adaptive control method is introduced to further enhance the inertial behavior of the AVSG control.Firstly,a theoretical analysis is conducted on the various components of the DC microgrid,the structure of analogous virtual synchronous generator,and the control structure’s main parameters related to the DC microgrid’s inertial behavior.Secondly,the voltage change rate tracking coefficient is introduced to adjust the change of the virtual capacitance and damping coefficient flexibility,which further strengthens the inertia trend of the DC microgrid.Additionally,a small-signal modeling approach is used to analyze the approximate range of the AVSG’s main parameters ensuring system stability.Finally,conduct a simulation analysis by building the model of the DC microgrid system with photovoltaic(PV)and battery energy storage(BES)in MATLAB/Simulink.Simulation results from different scenarios have verified that the AVSG control introduces fixed inertia and damping into the droop control of the battery,resulting in a certain level of inertia enhancement.Furthermore,the additional adaptive control strategy built upon the AVSG control provides better and flexible inertial support for the DC microgrid,further enhances the stability of the DC bus voltage,and has a more positive impact on the battery performance.

Application of Power Electronics Converters in Renewable Energy

Against the backdrop of global energy shortages and increasingly severe environmental pollution,renewable energy is gradually becoming a significant direction for future energy development.Power electronics converters,as the core technology for energy conversion and control,play a crucial role in enhancing the efficiency and stability of renewable energy systems.This paper explores the basic principles and functions of power electronics converters and their specific applications in photovoltaic power generation,wind power generation,and energy storage systems.Additionally,it analyzes the current innovations in high-efficiency energy conversion,multilevel conversion technology,and the application of new materials and devices.By studying these technologies,the aim is to promote the widespread application of power electronics converters in renewable energy systems and provide theoretical and technical support for achieving sustainable energy development.

V型折叠颏下皮瓣修复口腔缺损的临床应用

目的:口腔中小型缺损修复一直是一个难题,游离皮瓣和远位带蒂组织瓣难以满足临床需求,传统颏下皮瓣存在供区损伤风险。本研究旨在探讨V型折叠颏下皮瓣修复口腔中小型缺损的疗效。方法:回顾性分析2019年3月至2022年12月中南大学湘雅三医院口腔科收治的28例口腔黏膜病变患者的临床资料。根据颏下皮瓣手术方式的不同将患者分为V型折叠组(17例)和传统组(11例),V型折叠组采用V型折叠颏下皮瓣进行术后软组织修复,传统组采用传统颏下皮瓣进行修复,患者术后随访6~48个月。比较2组皮瓣存活情况、皮瓣修复时间以及皮瓣修复效果。结果:2组皮瓣存活率、皮瓣大小、制瓣时间、修复手术时间和术后住院时间差异均无统计学意义(均P>0.05)。术后6个月,V型折叠组无抬头困难和下唇外翻,无皮肤“猫耳”畸形,疤痕隐藏于下颌骨下缘处,创面美观度、功能评分均明显高于传统组(均P<0.05)。结论:V型折叠颏下皮瓣具有设计灵活、制备简单、血供可靠、保护供区等优点,可以有效维持颏部美观和避免功能障碍。

鼠疫耶尔森菌低钙应答V抗原人源单克隆抗体筛选与鉴定

目的筛选抗低钙应答V抗原(LcrV)的人源单克隆抗体,分析抗体基因特点,检测抗体与抗原结合的特异性、亲和力及功能。方法使用PCR方法从鼠疫疫苗临床试验志愿者外周血细胞cDNA扩增抗体轻、重链可变区基因片段,构建ScFv噬菌体抗体文库。用LcrV对文库进行富集筛选,将获得的抗体基因表达成人IgG1后,测定抗体与LcrV抗原结合的特异性、亲和力、免疫调节功能以及保护能力。结果成功构建了鼠疫耶尔森菌人源ScFv抗体文库,库容量为7.54×10^(8)。抗体文库经LcrV筛选后获得了3株抗LcrV抗体,命名为RV-B4、RV-D1和RV-E8。3株抗体重链为VH1-46和VH3-30,轻链分别为VL1-51、VK3-20和VK1-39。3株抗体经ELISA及Western blot验证均与LcrV特异性结合,其与V抗原结合的解离常数(KD)分别为2.1 nmol/L、1.24 nmol/L和42 nmol/L。RV-D1体外降低人THP-1分泌TNF-α,动物攻毒试验中未发现抗LcrV抗体的保护效果。结论从鼠疫疫苗免疫人群获得了靶向低钙应答V抗原的人源抗体,以结合抗体为主,不能有效阻断病菌感染。所获得的单抗为鼠疫免疫基础研究、鼠疫诊断应用提供了候选材料。

基于CRV-YOLO的苹果中心花和边花识别方法

苹果树疏花是果园生产管理中的重要环节。准确高效地识别苹果中心花和边花,是研发智能疏花机器人的前提。针对苹果疏花作业中的实际需求,提出了一种基于CRV-YOLO的苹果中心花和边花识别方法。本文基于YOLO v5s模型进行了如下改进:将C-CoTCSP结构融入Backbone,更好地学习上下文信息并提高了模型特征提取能力,提高了模型对外形相似和位置关系不明显的中心花和边花的检测性能。在Backbone中添加改进RFB结构,扩大特征提取感受野并对分支贡献度进行加权,更好地利用了不同尺度特征。采用VariFocal Loss损失函数,提高了模型对遮挡等场景下难识别样本检测能力。在3个品种1837幅图像数据集上进行了实验,结果表明,CRV-YOLO的精确率、召回率和平均精度均值分别为95.6%、92.9%和96.9%,与原模型相比,分别提高3.7、4.3、3.9个百分点,模型受光照变化和苹果品种影响较小。与Faster R-CNN、SSD、YOLOX、YOLO v7模型相比,CRV-YOLO的精确率、平均精度均值、模型内存占用量和复杂度性能最优,召回率接近最优。研究成果可为苹果智能疏花提供技术支持。

V区温度及螺杆转速对挤压膨化牛肉制品理化性质的影响

利用挤压膨化技术加工高粱-山药-牛肉混合物料,以水溶性、吸水性、色差值、粒度、质构、膨化度、糊化度及红外光谱各基团峰值为指标,分析V区温度和螺杆转速对挤压膨化产品理化性质的影响。结果表明,提高V区温度能够提高产品的红度值、黄度值、硬度、脆度和咀嚼度,吸水性、水溶性、粒度、膨化度、糊化度及红外光谱各基团峰值呈先上升后下降的趋势,亮度值显著下降(P<0.05);同时,随着螺杆转速的提升,产品的红度值、黄度值和糊化度显著下降(P<0.05),吸水性、水溶性、粒度和膨化度呈先上升后下降的趋势,硬度、脆度、咀嚼度及红外光谱各基团峰值先下降后上升,亮度值显著下降(P<0.05)。综合理化指标确定V区温度最佳值为150℃,螺杆转速最佳值为160 r/min。

基于ⅢF A/V规范和Avalon系统的大学图书馆视听数据库建设研究

随着中国网络基础设施的不断改善,视听媒体在年轻一代中非常流行,给以文本资源为主的图书馆带来了挑战。本研究旨在探究国内外大学图书馆视听资源数据库建设的现状,借鉴ⅢF规范在图像资源管理方面的成功经验和各种视听保存社区的实践,提出基于ⅢF A/V规范与开源软件的中国大学图书馆视听资源管理方法。通过分析华东师范大学图书馆在视听资源保存、流媒体发布、时间轴气泡注释、转录、视听结构化和开放共享方面的实践,进行实证研究。

罗汉果皂苷V对RSL3诱导的SH-SY5Y细胞铁死亡的抑制作用及其机制

目的:探讨罗汉果皂苷V(MV)对铁死亡诱导剂RAS选择性致死分子3(RSL3)诱导的人神经母细胞瘤SH-SY5Y细胞铁死亡的抑制作用及可能机制。方法:用RSL3诱导SH-SY5Y细胞建立铁死亡模型。MTT法检测细胞活力;倒置显微镜观察细胞形态;亚铁离子荧光探针FerroFarRed检测细胞内亚铁离子含量;线粒体红色荧光探针MitoTracker Red CMXRos检测线粒体膜电位(MMP);超氧化物阴离子荧光探针二氢乙啶和线粒体超氧化物红色荧光探针MitoSoX Red分别检测细胞内和线粒体内活性氧(ROS)。微板法检测细胞谷胱甘肽(GSH)和丙二醛(MDA)水平。Western blot检测脂酰辅酶A合成酶长链家族成员4(ACSL4)、环加氧酶2(COX-2、)谷胱甘肽过氧化物酶4(GPX4)和溶质载体家族7成员11(SLC7A11)蛋白表达水平。分子对接技术预测MV与ACSL4、COX-2、GPX4和SLC7A11的靶向关系。结果:与control组相比,RSL3组SH-SY5Y细胞活力显著降低(P<0.01),细胞内亚铁离子含量、细胞内和线粒体内ROS水平及MDA水平显著升高(P<0.05或P<0.01),MMP和GSH水平显著降低(P<0.01),ACSL4和COX-2蛋白表达水平显著升高,而GPX4和SLC7A11蛋白表达水平显著降低(P<0.01),提示成功建立了细胞铁死亡模型。MV处理使细胞活力显著升高(P<0.05),细胞内亚铁离子含量、细胞内和线粒体内ROS水平及MDA水平显著降低(P<0.01),MMP和GSH水平显著升高(P<0.05或P<0.01);ACSL4和COX-2蛋白水平显著降低,而GPX4和SLC7A11蛋白水平显著升高(P<0.05或P<0.01)。分子对接结果显示,MV与铁死亡核心蛋白ACSL4、COX-2、GPX4和SLC7A11存在结合位点。结论:MV可抑制RSL3诱导的SH-SY5Y细胞铁死亡的发生,其机制可能与激活SLC7A11/GPX4和抑制ACSL4/COX-2有关。

不同V型辅助配体构筑的两个镉配位聚合物的合成、结构和荧光性质

在溶剂热条件下,合成了2个基于V型辅助配体(bipmo、bppmo)的镉配位聚合物{[Cd(bipmo)(NDC)]·1.75H_(2)O}_n (1)和{[Cd(bppmo)(NDC)(H_(2)O)]·H_(2)O}_(n) (2),其中H_(2)NDC=2,6-萘二羧酸,bipmo=双(4-(1H-咪唑-1-基)苯基)甲酮,bppmo=双(4-(吡啶-4-基)苯基)甲酮。利用单晶X射线衍射、键价和分析、红外光谱和元素分析对其结构进行了表征。研究发现,配合物1具有二重互穿的{6~3}拓扑结构。配合物2同样是3-连接的{6~3}拓扑,却存在三重穿插结构。分析表明,V型配体对最终结构的形成有很大影响。此外,对配合物1和2的发光性质也进行了详细研究。

基于V形记忆曲线的汽车轮毂造型衍生设计方法

为提高汽车轮毂造型设计的效率,增强品牌造型识别度,提出一种高效的轮毂造型衍生创新设计方法。通过分析轮毂V形记忆曲线的变化规律和特点,总结出两类V形记忆曲线,并根据控制点数量分别进行了定量化表达;以轮毂造型的5个设计要素为基础,提取轮毂骨骼的特征并建立运算法则,构建基于V形记忆曲线的轮毂造型衍生设计方法;通过参数化实现造型构建方案衍生,验证了该方法的可行性。研究结果表明:基于V形记忆曲线的轮毂造型衍生设计方法提高了轮毂设计效率,实现了轮毂造型创新及提升品牌特征延续的设计目标,为同类型产品的创新设计提供参考。

切向车铣18CrNiMo7-6钢V形缺口表面三维形貌研究

疲劳试样的缺口加工一直是一个亟需解决的难题。提出一种全新的切向车铣加工缺口工艺,通过理论与试验结合的方法探究了切向车铣对V形缺口表面形貌的影响规律。结果表明:在不同的工艺参数下,切向车铣V形缺口底部会呈现出不同的加工纹理方向,提出的理论公式计算出的纹理方向与工件轴线的夹角与实际结果误差很小;缺口底部三维表面粗糙度R_(a)随着铣刀转速n_(c)的升高而减小,随着工件转速n_(w)的升高而变大,随着每齿进给量f_(z)的增加先减小后增大;得到表面粗糙度最优参数组合为n_(c)=6000 r/min,n_(w)=100 r/min,f_(z)=0.0375μm,在此切削参数组合下得到的V形缺口表面粗糙度R_(a)可达到0.075μm。

甲状腺乳头状癌及其临床病理特征与Braf蛋白表达、BRAF V600E基因突变之间的关系

目的:观察甲状腺乳头状癌临床病理特征和BRAF V600E基因突变、BRAF蛋白表达的相关性。方法:临床选择2019年7月~2020年2月在深圳市龙岗中心医院就诊的原发性甲状腺乳头状癌患者100例,对其临床病理信息进行收集,PCR扩增后测序,分析甲状腺乳头状癌临床病理特征和BRAF V600E基因、Braf蛋白表达之间的关系。结果:BRAF V600E基因突变、Braf蛋白表达情况与肿瘤复发/远处转移、肿瘤分期、肿瘤大小、年龄等因素密切相关,差异有统计学意义(P<0.05);100例甲状腺乳头状癌出现钙化者为47例(47.0%),通常具有粗大片状钙化、砂砾样钙化,研究发现BRAF基因突变状态与钙化类型、是否出现钙化无明显相关性。结论:甲状腺乳头状癌的临床病理指标与BRAF V600E基因突变、Braf蛋白表达密切相关,是评估甲状腺乳头状癌的相关预后因子之一。

构式“越V越A”的准入条件探究

针对“越吃越胖”“越吃越瘦”可以成立,而“越吃越饱”不能成立的现象,需要探究“越V越A”中“V”“A”的准入条件,并考察“越……越……”如何与“V”“A”相互作用、共同形成“越V越A”构式义的问题。研究发现,“越V越A”中的“V”具有[+持续性]或[+重复性]特征,“A”具有[+离散性](扩展性或收缩性)、[-边界性]特征,这些语义特征需要结合语境去理解,在二者同时满足的情况下,言者使用“越V越A”来描写状态,而非叙述结果。

V槽的偏斜度及其分布对微通道流动沸腾特性的影响

运用VOF模型与用户自定义函数,数值模拟水在V槽微通道内的流动沸腾过程,分析V槽的偏斜度及其分布对通道内气泡的生长、脱离及气液两相流流型变化的影响.研究结果表明:V槽偏斜度会影响微气泡的脱离直径和脱离时间;偏斜度为1.0的V槽对应的气泡脱离直径较偏斜度为0和0.5的V槽分别减小0.063、0.025 mm,脱离时间分别缩短5.20、2.40 ms,气液两相流由泡状流向受限气泡流的转变沿流动方向往后推移2.7 mm,且片段环状流占据区间有所减小;对于偏斜度为1.0的V槽,当其沿流动方向的分布不同时,微通道内气液两相流动呈现不同特征,前疏后密的V槽分布能更好地抑制通道内气泡聚并,通道全程未见片段环状流发生,近壁区液膜可以避免受热面局部干涸,提升微通道流动沸腾换热的稳定性和可靠性.