Recently,Baranek et al.performed single-cell RNA sequencing(scRNA-seq)analysis and confirmed some of the transcriptomic results by in vitro cell culture with IL-7/IL-15 and flow cytometric analysis.2 The authors defin...Recently,Baranek et al.performed single-cell RNA sequencing(scRNA-seq)analysis and confirmed some of the transcriptomic results by in vitro cell culture with IL-7/IL-15 and flow cytometric analysis.2 The authors defined several intermediate stages,including iNKT2a(CD24−NK1.1−CD138−FR4−),iNKT2b(CD24−NK1.1−CD138−FR4+),and iNKT1a(CD24−Sca-1hiCD138-NK1.1dim),with various degrees of potential to differentiate into iNKT1,iNKT2,and iNKT17 subsets(Fig.1).展开更多
基金This work was supported by grants from the National Key Research and Development Program of China(2017YFA0104500)the National Natural Science Foundation of China(31671244,31872734,Q.G.)+2 种基金the Foundation for Innovative Research Groups of the National Natural Science Foundation of China(81621001)the Beijing Natural Science Foundation(7202079)the Non-Profit Central Research Institute Fund of the Chinese Academy of Medical Sciences(2018PT31039).
文摘Recently,Baranek et al.performed single-cell RNA sequencing(scRNA-seq)analysis and confirmed some of the transcriptomic results by in vitro cell culture with IL-7/IL-15 and flow cytometric analysis.2 The authors defined several intermediate stages,including iNKT2a(CD24−NK1.1−CD138−FR4−),iNKT2b(CD24−NK1.1−CD138−FR4+),and iNKT1a(CD24−Sca-1hiCD138-NK1.1dim),with various degrees of potential to differentiate into iNKT1,iNKT2,and iNKT17 subsets(Fig.1).
