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新型冷作模具钢9Cr5Mo2V不同热处理下的性能研究 被引量:1
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作者 魏金龙 吴红庆 吴晓春 《热加工工艺》 北大核心 2024年第2期30-34,40,共6页
以新型冷作模具钢9Cr5Mo2V为研究对象,通过淬回火温度对硬度的影响规律,选出5种不同的热处理工艺,对比不同工艺下模具钢的横、纵向冲击韧性、抗弯强度和耐磨性能,并与7Cr5Mo2V钢进行对照。结果表明,9Cr5Mo2V钢在1030℃淬火、520℃回火... 以新型冷作模具钢9Cr5Mo2V为研究对象,通过淬回火温度对硬度的影响规律,选出5种不同的热处理工艺,对比不同工艺下模具钢的横、纵向冲击韧性、抗弯强度和耐磨性能,并与7Cr5Mo2V钢进行对照。结果表明,9Cr5Mo2V钢在1030℃淬火、520℃回火热处理下具有极佳的硬度(63.6 HRC)和耐磨性能(260.16 min/mm3);970℃淬火、520℃回火热处理后,其纵向平均冲击功最高,达62.83 J;经970℃淬火、210℃回火热处理后,9Cr5Mo2V钢具有优良的抗弯性能,纵向抗弯强度为3621.9 MPa。9Cr5Mo2V钢的纵向冲击功和抗弯强度均高于横向的纵向冲击功和抗弯强度。 展开更多
关键词 9Cr5Mo2v 热处理 冲击韧性 抗弯强度 耐磨性
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Frequency of the C677T Polymorphism of MTHFR, G20210A of Prothrombin and R506Q of Factor V Leiden in Type 2 Diabetics in Abidjan
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作者 N’Guessan-Blao Rebecca Yapo Vincent +3 位作者 Yayo-Ayé Mireille Adjambri Eusèbe Koné-Koné Fatoumata Sawadogo Duni 《Open Journal of Genetics》 CAS 2024年第1期13-25,共13页
In Africa, the prevalence of diabetes is escalating and remains a concern due to the numerous complications it causes. Vascular damage associated with diabetes leads to a prothrombotic state observed in diabetic indiv... In Africa, the prevalence of diabetes is escalating and remains a concern due to the numerous complications it causes. Vascular damage associated with diabetes leads to a prothrombotic state observed in diabetic individuals. Diabetes is a complex and multifactorial disease involving genetic components. With the aim of preventing complications and contributing to an efficient management of diabetes, we investigated genes likely to lead to a risk of thrombosis, in particular the C677T of MTHFR, G20210A of prothrombin, and R506Q of factor V Leiden in type 2 diabetics in Abidjan receiving ambulatory care. A descriptive cross-sectional study was carried out on consenting type 2 diabetic patients. Mutation detection was carried out using the PCR-RFLP method employing restriction enzymes. Hemostasis tests (fibrinogen, D-dimers, fibrin monomers, and von Willebrand factor) were performed using citrate tubes on the Stage? Star Max automated system. Plasminogen activator inhibitor was assayed by ELISA method, and biochemical parameters were determined using the COBAS C311. The study population consisted of 45 diabetic patients, 51.1% of whom presented vascular complications, mainly neuropathy. Disturbances in hemostasis parameters were observed, with 15.5% of patients showing an increase in fibrin monomers. Mutation analysis revealed an absence of factor V mutation (factor V Leiden) and of G20210A mutation of the prothrombin gene. However, 15.6% of subjects had a heterozygous C677T mutation of MTHFR, with 57% of them being anemic. The exploration of biological and genetic factors associated with thrombotic risk is of significant interest in the optimal management of African type 2 diabetics. 展开更多
关键词 type 2 Diabetes C677t of MtHFR G20210A Factor v Leiden thrombosis
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乳腺癌患者不同病理特征中H3K9ac、PD-L1、VISTA表达及其联合检测对乳腺癌不良预后的预测价值
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作者 赵杰 陶璐 刘姗姗 《河南医学研究》 CAS 2024年第18期3357-3361,共5页
目的探讨乳腺癌患者不同病理特征中组蛋白H3第9位赖氨酸残基乙酰化(H3K9ac)、程序性死亡因子配体1(PD-L1)、T细胞激活抑制物免疫球蛋白的可变区结构域(VISTA)及其联合检测对乳腺癌不良预后的预测价值。方法选取2018年5月至2020年7月平... 目的探讨乳腺癌患者不同病理特征中组蛋白H3第9位赖氨酸残基乙酰化(H3K9ac)、程序性死亡因子配体1(PD-L1)、T细胞激活抑制物免疫球蛋白的可变区结构域(VISTA)及其联合检测对乳腺癌不良预后的预测价值。方法选取2018年5月至2020年7月平顶山市第一人民医院收治的130例乳腺癌患者,免疫组化检测乳腺癌组织及癌旁组织中H3K9ac、PD-L1及VISTA表达情况。比较乳腺癌组织、癌旁组织中H3K9ac、PD-L1及VISTA表达情况;比较乳腺癌不同病理特征中H3K9ac、PD-L1及VISTA表达情况;随访3 a,根据是否发生转移、复发、死亡分为预后不良和预后良好;比较预后不良和预后良好乳腺癌患者H3K9ac、PD-L1及VISTA表达情况;分析H3K9ac、PD-L1、VISTA表达联合检测对乳腺癌不良预后的预测价值。结果乳腺癌组织中H3K9ac、PD-L1、VISTA高表达率均高于癌旁组织(P<0.05);不同组织分化程度、T分期、N分期、阳性淋巴细胞数目中H3K9ac、PD-L1、VISTA高表达率比较,差异有统计学意义(P<0.05);随访3 a,130例乳腺癌患者失访7例(5.38%),预后不良22例,预后良好101例。预后不良患者H3K9ac、PD-L1、VISTA高表达率高于预后良好患者(P<0.05);H3K9ac、PD-L1、VISTA联合检测预测乳腺癌患者不良预后的AUC为0.902,大于各指标单独预测的0.714、0.866、0.781(P<0.05)。结论乳腺癌患者癌组织中H3K9ac、PD-L1及VISTA均呈高表达,其高表达与分化程度、分期、淋巴细胞数目等病理特征相关,H3K9ac、PD-L1、VISTA联合检测对乳腺癌患者不良预后具有较高的预测价值。 展开更多
关键词 乳腺癌 组蛋白H3第9位赖氨酸残基乙酰化 程序性死亡因子配体1 t细胞激活抑制物免疫球蛋白的可变区结构域 预后
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Antigen-presenting effects of effector memory Vγ9Vδ2 T cells in rheumatoid arthritis 被引量:18
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作者 Chaoying Hu Liu Qian +11 位作者 Yi Miao Qiuyu Huang Ping Miao Ping Wang Qiwen Yu Hong Nie Jiying Zhang Dongyi He Rong Xu Xuehua Chen Bingya Liu Dongqing Zhang 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2012年第3期245-254,共10页
Rheumatoid arthritis is an autoimmune disease that primarily affects the limbs, but the pathogenic mechanism remains unclear. 78 T cells, a T-cell subpopulation, are characterized by multiple biological functions and ... Rheumatoid arthritis is an autoimmune disease that primarily affects the limbs, but the pathogenic mechanism remains unclear. 78 T cells, a T-cell subpopulation, are characterized by multiple biological functions and associated with a variety of diseases. This study investigated the antigen-presenting effects of γδ2 cells and their relationship with rheumatoid arthritis development. We found that Vγ9Vδ2 T cells (the predominant subtype of γδ T cells in peripheral blood) were activated by isopentenyl pyrophosphate to continuously proliferate and differentiate into effector memory cells. The effector memory Vγ9Vδ2 T cells exhibited phenotypic characteristics of specific antigen-presenting cells, including high HLA-DR and CD80/86 expression. These Vγ9Vδ2 T cells could present soluble antigens and synthetic peptides to CD4+ T cells. Vγ9Vδ2 T cells with different phenotypes showed different cytokine secretion patterns. Effector memoryVγ9Vδ2 T cells simultaneously secreted not only interferon (IFN)-γbut also IL-17. The peripheral blood and joint synovial fluid from RA patients contained numerous heterogeneous γδ T cells that were predominantly effector memory Vγ9Vδ2 T cells with the ability to secrete inflammatory factors. We also found that γδ T cells had a similar antigen-presenting capability to B cells. These results suggest that during the development of rheumatoid arthritis, 78 T cells can aggravate immune dysfunction and produce abnormal immune damage by secreting cytokines and inducing inflammatory cells to participate in synergistic inflammatory responses. Furthermore, γδ T cells can behave similarly to B cells to present viral peptides and autoantigen peptides to CD4+ T cells, thus sustaining CD4+ T-cell activation. 展开更多
关键词 antigen-presenting function rheumatoid arthritis γδt cell tEM 92 t cell
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Self-activation of Vγ9Vδ2 T cells by exogenous phosphoantigens involves TCR and butyrophilins
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作者 ChloéLaplagne Laetitia Ligat +5 位作者 Juliet Foote Frederic Lopez Jean-Jacques Fournié Camille Laurent Salvatore Valitutti Mary Poupot 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2021年第8期1861-1870,共10页
The high cytotoxic activity of Vγ9Vδ2 T lymphocytes against tumor cells makes them useful candidates in anticancer therapies.However,the molecular mechanism of their activation by phosphoantigens(PAgs)is not complet... The high cytotoxic activity of Vγ9Vδ2 T lymphocytes against tumor cells makes them useful candidates in anticancer therapies.However,the molecular mechanism of their activation by phosphoantigens(PAgs)is not completely known.Many studies have depicted the mechanism of Vγ9Vδ2 T-cell activation by PAg-sensed accessory cells,such as immune presenting cells or tumor cells.In this study,we demonstrated that pure resting Vγ9Vδ2 T lymphocytes can self-activate through exogenous PAgs,involving their TCR and the butyrophilins BTN3A1 and BTN2A1.This is the first time that these three molecules,concurrently expressed at the plasma membrane of Vγ9Vδ2 T cells,have been shown to be involved together on the same and unique T cell during PAg activation.Moreover,the use of probucol to stimulate the inhibition of this self-activation prompted us to propose that ABCA-1 could be implicated in the transfer of exogenous PAgs inside Vγ9Vδ2 T cells before activating them through membrane clusters formed byγ9TCR,BTN3A1 and BTN2A1.The self-activation of Vγ9Vδ2 T cells,which leads to self-killing,can therefore participate in the failure ofγδT cell-based therapies with exogenous PAgs and should be taken into account. 展开更多
关键词 92 t cells Phosphoantigen Butyrophilins t-cell receptor
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2型糖尿病合并骨质疏松患者血清miR-9-5p和NFAT5的表达及其与骨折的关系
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作者 温聪慧 杨营军 +5 位作者 殷璐 许玲玉 马伟民 黄婷 吕朝阳 徐在革 《临床与病理杂志》 CAS 2024年第3期345-353,共9页
目的:2型糖尿病(type 2 diabetes mellitus,T2DM)是一种多病因代谢性疾病,骨质疏松(osteoporosis,OP)和骨折是其常见并发症。本研究旨在探讨T2DM合并OP患者血清中微RNA(microRNA,miR)-9-5p和核转录因子5(nuclear factor of activated T-... 目的:2型糖尿病(type 2 diabetes mellitus,T2DM)是一种多病因代谢性疾病,骨质疏松(osteoporosis,OP)和骨折是其常见并发症。本研究旨在探讨T2DM合并OP患者血清中微RNA(microRNA,miR)-9-5p和核转录因子5(nuclear factor of activated T-cells 5,NFAT5)的表达水平,以及其与骨折的关系。方法:收集郑州市第七人民医院就诊的T2DM合并OP患者184例(OP组),另纳入同时间段单纯T2DM患者184例(T2DM组)。实时聚合酶链反应检测血清miR-9-5p、NFAT5表达水平。随访2年,根据新发骨折情况,将T2DM合并OP患者分为骨折组(43例)与无骨折组(141例)。Pearson法分析血清miR-9-5p、NFAT5分别与空腹血糖(fasting plasma glucose,FPG)、I型前胶原N末端前肽(procollagen I N-terminal propeptide,PINP)、空腹胰岛素(fasting insulin,FINS)、胰岛素抵抗指数(insulin resistance index,HOMA-IR)、骨密度T值、I型胶原羧基端β降解产物(type I collagen hydroxy terminal peptideβdegradation products,β-CTX)相关性,以及miR-9-5p与NFAT5的相关性;采用受试者操作特征(receiver operator characteristic,ROC)曲线评估血清miR-9-5p、NFAT5对T2DM合并OP患者骨折的预测价值,多因素logistic回归分析T2DM合并OP患者骨折的影响因素。结果:OP组血清miR-9-5p水平高于T2DM组,NFAT5水平低于T2DM组(均P<0.05)。与无骨折组相比,骨折组患者糖尿病病程、FPG、HOMA-IR、β-CTX、miR-9-5p水平均升高,而PINP、NFAT5水平均降低(均P<0.05)。骨折患者血清miR-9-5p与NFAT5水平呈负相关(r=−0.716,P<0.05);miR-9-5p水平与FPG、HOMA-IR、β-CTX均呈正相关,与PINP呈负相关(均P<0.05),而血清NFAT5水平与FPG、HOMA-IR、β-CTX均呈负相关,与PINP呈正相关(均P<0.05)。血清miR-9-5p、NFAT5单一预测T2DM合并OP患者骨折风险的曲线下面积(area under curve,AUC)分别为0.878和0.868,联合预测的AUC为0.933。β-CTX、miR-9-5p为T2DM合并OP患者骨折的危险因素,PINP、NFAT5为T2DM合并OP患者骨折的保护因素(均P<0.05)。结论:T2DM合并OP患者血清miR-9-5p表达水平升高,NFAT5表达水平降低,两者与骨折发生均有一定关系,miR-9-5p联合NFAT5对骨折预测价值更高。 展开更多
关键词 2型糖尿病 骨质疏松 微RNA-9-5p 核转录因子5 骨折
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体外扩增的Vγ9Vδ2T细胞抗H1N1流感病毒效应的研究 被引量:1
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作者 冯婷 巩天祥 +3 位作者 吴啟洪 朱婷婷 陶于洪 李虹 《免疫学杂志》 CAS CSCD 北大核心 2016年第7期553-558,共6页
目的探讨帕米磷酸钠(PAM)体外扩增培养的人外周血Vγ9Vδ2 T细胞对感染流感病毒H1N1的人肺上皮细胞A549的细胞毒效应。方法 PAM和IL-2联合刺激培养健康成人外周血单个核细胞(PBMC)10~14 d,免疫磁珠分选法纯化得到Vγ9Vδ2 T细胞,再... 目的探讨帕米磷酸钠(PAM)体外扩增培养的人外周血Vγ9Vδ2 T细胞对感染流感病毒H1N1的人肺上皮细胞A549的细胞毒效应。方法 PAM和IL-2联合刺激培养健康成人外周血单个核细胞(PBMC)10~14 d,免疫磁珠分选法纯化得到Vγ9Vδ2 T细胞,再将Vγ9Vδ2 T细胞与感染流感病毒的A549细胞按不同的效靶比(E∶T)接触共育或按E∶T=20∶1非接触共育6 h。流式细胞分析术检测A549的存活率及Vγ9Vδ2 T细胞穿孔素(perforin)的表达情况,收集培养上清检测流感病毒的滴度,提取培养上清及A549细胞的总RNA,实时荧光定量检测流感病毒基质蛋白M1基因的表达情况。结果 PAM在体外能有效活化并扩增Vγ9Vδ2 T细胞。Vγ9Vδ2 T细胞对感染流感病毒的A549细胞具有较强的杀伤作用,且随E∶T的升高而增大,在E∶T=20∶1时,Vγ9Vδ2 T细胞能杀伤60%的感染了流感病毒的A549细胞。接触共育组的A549细胞杀伤率明显高于非接触共育组(P〈0.05)。Vγ9Vδ2 T细胞经流感病毒感染后Perforin的表达明显上调。接触共育组的病毒复制也明显受到抑制(P〈0.05)。结论 PAM刺激扩增的Vγ9Vδ2 T细胞能有效杀伤感染流感病毒的人肺上皮细胞A549,其效应机制依赖于两者细胞间的接触。 展开更多
关键词 92t细胞 帕米磷酸钠 流感病毒 A549细胞
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嗜乳脂蛋白3A1(BTN3A1)促进结核杆菌耐热抗原诱导的人外周血Vγ9Vδ2 T细胞活化增殖 被引量:1
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作者 唐洁 孙静 +6 位作者 查成 常见荣 胡坤 方强 陈雨晴 陈静 李柏青 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2020年第8期680-686,共7页
目的初步探讨嗜乳脂蛋白3A1(BTN3A1)在结核杆菌耐热抗原(MTB-HAg)诱导的人外周血Vγ9Vδ2 T细胞活化增殖中的作用。方法人外周血单个核细胞(PBMC)先加BTN3A1阻断性抗体作用3 h,再分别用MTB-HAg和磷酸类抗原(PAg)刺激培养24 h后收集细胞... 目的初步探讨嗜乳脂蛋白3A1(BTN3A1)在结核杆菌耐热抗原(MTB-HAg)诱导的人外周血Vγ9Vδ2 T细胞活化增殖中的作用。方法人外周血单个核细胞(PBMC)先加BTN3A1阻断性抗体作用3 h,再分别用MTB-HAg和磷酸类抗原(PAg)刺激培养24 h后收集细胞,用流式细胞术检测Vγ9Vδ2 T细胞中CD69表达;或刺激20 h后收集细胞,检测Vγ9Vδ2 T细胞中1型辅助性T(Th1)细胞因子γ干扰素(IFN-γ)和肿瘤坏死因子α(TNF-α)产生细胞比例;或刺激后,在含白细胞介素2(IL-2)培养液中培养10 d后,收集细胞检测Vγ9Vδ2 T细胞扩增比例及增殖活性。结果MTB-HAg刺激后,Vγ9Vδ2 T细胞中CD69平均荧光强度和阳性细胞比例在BTN3A1阻断组均明显下降(为刺激组的13.84%和43.00%);而PAg阻断组的CD69平均荧光强度和阳性细胞比例显著被抑制(为刺激组的3.10%,4.47%和9.53%,10.91%)。MTB-HAg刺激后Vγ9Vδ2 T细胞中IFN-γ和TNF-α产生细胞比例在BTN3A1阻断组显著减少,Vγ9Vδ2 T细胞扩增数量和细胞增殖活性在BTN3A1阻断组也显著减少或降低。与PAg阻断组明显被抑制的结果相似。结论BTN3A1促进MTB-HAg诱导的外周血Vγ9Vδ2 T细胞活化增殖。 展开更多
关键词 嗜乳脂蛋白3A1(BtN3A1) 结核杆菌耐热抗原(MtB-HAg) 磷酸类抗原(PAg) 92 t细胞
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脂蛋白相关磷脂酶A2基因I198T和V279F多态性与冠状动脉粥样硬化病变程度的相关性 被引量:8
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作者 张梦遥 洪梅 鲁翔 《南京医科大学学报(自然科学版)》 CAS CSCD 北大核心 2015年第3期380-384,共5页
目的 :探讨脂蛋白相关磷脂酶A2(Lp-PLA2)基因I198T、V279F多态性与冠状动脉粥样硬化病变严重程度和稳定性的相关性。方法:纳入268例冠状动脉粥样硬化患者及113例冠脉造影完全正常者,应用DNA测序仪检测Lp-PLA2基因I198T和V279F多态性,并... 目的 :探讨脂蛋白相关磷脂酶A2(Lp-PLA2)基因I198T、V279F多态性与冠状动脉粥样硬化病变严重程度和稳定性的相关性。方法:纳入268例冠状动脉粥样硬化患者及113例冠脉造影完全正常者,应用DNA测序仪检测Lp-PLA2基因I198T和V279F多态性,并进行统计学分析。结果:按照Gensini积分及冠心病临床分型,所分亚组的I198T和V279F基因型频率及其等位基因频率与对照组无差异;单支冠脉病变者IT+TT基因型和VF+FF基因型及其等位基因F频率高于对照组,而多支病变者则与对照组无差异。亦未发现I198T和V279F不同基因型在血脂水平及高血压、糖尿病患者比例上有差别。结论:未能发现Lp-PLA2基因I198T和V279F变异与冠状动脉粥样硬化病变严重程度和稳定性的显著相关性。 展开更多
关键词 脂蛋白相关性磷脂酶A2 I198t v279F 冠状动脉粥样硬化 冠心病
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HSP70刺激人Vγ9Vδ2 T细胞CCL5/RANTES的分泌
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作者 陈礼文 张建军 +3 位作者 宇芙蓉 桂丽 张发苏 曹元应 《免疫学杂志》 CAS CSCD 北大核心 2008年第6期644-646,共3页
目的探讨热休克蛋白70(HSP70)体外诱导人外周血单个核细胞(PBMCs)后CC型趋化因子CCL5/RANTES在Vγ9Vδ2 T细胞表达。方法人PBMCs在体外用重组HSP70诱导活化,在第1、4、7、10、14天分别收集细胞,流式细胞术胞内细胞因子分析技术检测表达C... 目的探讨热休克蛋白70(HSP70)体外诱导人外周血单个核细胞(PBMCs)后CC型趋化因子CCL5/RANTES在Vγ9Vδ2 T细胞表达。方法人PBMCs在体外用重组HSP70诱导活化,在第1、4、7、10、14天分别收集细胞,流式细胞术胞内细胞因子分析技术检测表达CCL5/RANTES的Vγ9Vδ2 T细胞百分率,并设结核杆菌低相对分子质量抗原(Mtb-Ag)刺激组作为对照。结果HSP70诱导组分泌CCL5/RANTES的Vγ9Vδ2 T细胞百分率在第1、4、7、10、14天分别为5.9±1.7、16.2±2.5、22.8±3.7、32.4±3.6与57.8±7.2;Mtb-Ag诱导组分别为3.7±2.5、10.5±1.8、11.1±2.3、26.3±3.9与47±4.7;HSP诱导Vγ9Vδ2T分泌CCL5/RANTES的能力比Mtb-Ag强。结论HSP70特异性诱导Vγ9Vδ2 T细胞增殖并分泌CCL5/RANTES,表明二者协同作用,共同参与免疫应答并可能介导某些炎症反应。 展开更多
关键词 热休克蛋白70 趋化因子 92 t细胞
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The Cytomegalovirus Enhancer Induces an Immediate Response to the Myosin Light Chain 2v Promoter during P19CL6 Cell Differentiation
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作者 Takanari Wakayama Kazuaki Ohashi +1 位作者 Yasuyuki Fujimoto Masatomo Maeda 《American Journal of Molecular Biology》 2017年第4期190-203,共14页
The P19CL6 mouse embryonic carcinoma cells efficiently differentiate into cardiac muscle cells in the presence of DMSO. A reporter plasmid for cardiac muscle differentiation was constructed by connecting the CMV enhan... The P19CL6 mouse embryonic carcinoma cells efficiently differentiate into cardiac muscle cells in the presence of DMSO. A reporter plasmid for cardiac muscle differentiation was constructed by connecting the CMV enhancer and a 250 bp MLC-2v promoter in front of the GFP gene to further evaluate the role of the CMV enhancer. This plasmid (pCBVenh/MLC-2vpro/EGFP) was stably introduced into P19CL6 cells, and the transfectant differentiated into cardiomyocytes with DMSO. Upon DMSO addition, GFP was immediately transcribed (within 2 days) and the amount of the transcript increased with cultivation. Concomitantly, GFP fluorescence was detected in the cells under a microscope. However, native MLC-2v was transcribed later on day 4. This expression time course is different from that of GFP. Clearly the CMV enhancer responded immediately to DMSO. Since GATA DNA-binding proteins play crucial roles in the initiation of cardiomyocyte differentiation, such a response could be ascribed to the presence of multiple GATA motifs in the enhancer sequence but not in the native MLC-2v promoter. Thus the CMV enhancer may be not only useful for gene therapy and monitoring cell differentiation but also the study of the role of GATA transcription factors expressed in P19CL6 cells. 展开更多
关键词 CYtOMEGALOvIRUS ENHANCER Differentiation GAtA tRANSCRIPtION Factor Gene Expression Heart MUSCLE MLC-2v P19CL6 cells PROMOtER
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肝癌患者与健康人外周血Vγ9Vδ2T细胞扩增前后比例及分化亚型对比
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作者 马俊 赵浩亮 +2 位作者 田伟 贺杰峰 李高鹏 《当代医学》 2016年第7期1-3,共3页
目的探讨肝细胞癌(HCC)患者外周血的Vγ9Vδ2T细胞比例,成熟分化亚型与健康人的差别,经体外扩增后上述指标的变化。方法选取健康捐献者(n=10)和HCC患者(n=20)的外周血共10 mL,采用不连续密度梯度离心法分离单核细胞层,借助流式细... 目的探讨肝细胞癌(HCC)患者外周血的Vγ9Vδ2T细胞比例,成熟分化亚型与健康人的差别,经体外扩增后上述指标的变化。方法选取健康捐献者(n=10)和HCC患者(n=20)的外周血共10 mL,采用不连续密度梯度离心法分离单核细胞层,借助流式细胞术检测Vγ9Vδ2T细胞占总T细胞比例,成熟分化亚型,并在体外加入唑来膦酸和IL-2进行扩增培养,12~14 d后收集细胞再次检测上述指标。结果培养前HCC组患者外周血中Vγ9Vδ2T细胞占总T细胞比例的平均值比健康人低(P〈0.05,P=0.009),在经过体外扩增培养后其比例明显升高(P〈0.0001),而在体外扩增后患者与健康人的Vγ9Vδ2T细胞比例差异无统计学意义(P〉0.05,P=0.3408)。患者Vγ9Vδ2T细胞的分化与成熟亚型作体外培养的前后对比发现,培养后Tn、Tcm、Temra的比例较培养前有明显下降(P〈0.05,P=0.0045,P=0.0236,P=0.0162),患者和健康人Tem比值均较培养前明显升高(P〈0.0001)。而在扩增前、扩增后患者和健康人的Vγ9Vδ2T细胞的成熟分化比例差异均无统计学意义。结论肝癌患者外周血Vγ9Vδ2T细胞比例虽然低于健康人,但其分化成熟亚型的分布比例与健康人相似,且经过体外扩增培养后Vγ9Vδ2T细胞和总效应细胞比例,均与健康人无差异。其增殖功能得到恢复,肝癌患者和健康人中应用这种体外扩增方法外周血Vγ9Vδ2T细胞的效果相似。 展开更多
关键词 肝细胞癌 92t细胞 过继免疫治疗 唑来膦酸
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V_2O_5添加对BaTi_4O_9微波介电陶瓷性能影响
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作者 蔡绍 严利 徐国跃 《中国陶瓷》 CAS CSCD 北大核心 2009年第12期25-27,共3页
研究V2O5添加对BaTi4O9微波介电陶瓷的烧结性能及介电特性的影响,试验结果表明由于V2O5作为低熔点氧化物的烧结助剂可以有效降低烧结温度,同时由于V5+离子的价位抑制作用可使BaTi4O9微波介电陶瓷保持良好的介电特性,当添加量为1.0wt%、1... 研究V2O5添加对BaTi4O9微波介电陶瓷的烧结性能及介电特性的影响,试验结果表明由于V2O5作为低熔点氧化物的烧结助剂可以有效降低烧结温度,同时由于V5+离子的价位抑制作用可使BaTi4O9微波介电陶瓷保持良好的介电特性,当添加量为1.0wt%、1200℃烧结时的介电特性为(2.5GHz):εr=41.4,tanδ=7×10-4,τf=50ppm/℃。 展开更多
关键词 Bati4O9 v2O5 微波介质陶瓷 低温烧结
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Container Based Nomadic Vehicular Cloud Using Cell Transmission Model
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作者 Devakirubai Navulkumar Menakadevi Thangavelu 《Computer Systems Science & Engineering》 SCIE EI 2023年第1期423-440,共18页
Nomadic Vehicular Cloud(NVC)is envisaged in this work.The predo-minant aspects of NVC is,it moves along with the vehicle that initiates it and functions only with the resources of moving vehicles on the heavy traffic ... Nomadic Vehicular Cloud(NVC)is envisaged in this work.The predo-minant aspects of NVC is,it moves along with the vehicle that initiates it and functions only with the resources of moving vehicles on the heavy traffic road without relying on any of the static infrastructure and NVC decides the initiation time of container migration using cell transmission model(CTM).Containers are used in the place of Virtual Machines(VM),as containers’features are very apt to NVC’s dynamic environment.The specifications of 5G NR V2X PC5 interface are applied to NVC,for the feature of not relying on the network coverage.Nowa-days,the peak traffic on the road and the bottlenecks due to it are inevitable,which are seen here as the benefits for VC in terms of resource availability and residual in-network time.The speed range of high-end vehicles poses the issue of dis-connectivity among VC participants,that results the container migration failure.As the entire VC participants are on the move,to maintain proximity of the containers hosted by them,estimating their movements plays a vital role.To infer the vehicle movements on the road stretch and initiate the container migration prior enough to avoid the migration failure due to vehicles dynamicity,this paper proposes to apply the CTM to the container based and 5G NR V2X enabled NVC.The simulation results show that there is a significant increase in the success rate of vehicular cloud in terms of successful container migrations. 展开更多
关键词 vehicular cloud container migration cell transmission model 5G NR v2X PC5 interface
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Effects of V2O5 on Proliferation and Differentiation of Limb Bud Cells of Rat
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作者 张天宝 孙棉龄 《Developmental and Reproductive Biology》 1997年第2期39-45,共7页
The micromass culture was used to determine the effects of vanadium pentoxide (V2O5 ) on the proliferation and differentiation of limb bud cells of rat. In the in vitro test, the results showed that V2O5 had obvious i... The micromass culture was used to determine the effects of vanadium pentoxide (V2O5 ) on the proliferation and differentiation of limb bud cells of rat. In the in vitro test, the results showed that V2O5 had obvious inhibiting effects on both proliferation and differentiation of limb bud cells with a dosedependent response, its proliferating and differentiating IC50 being 13.64 and 4.77μmol/L, respectively. In the in vivo/in vitro test, the results showed that V2O5 had no obvious effect on cell proliferation but had obvious inhibiting effect on cell differentiation. These results indicated that V2O5 might have a specific inhibiting effect on the differentiation of limb bud cells. 展开更多
关键词 micromass culture limb bud cells developmental toxicity v2O5 rat.
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寻常型银屑病和汗孔角化症患者外周血Vγ9Vδ2 T细胞的表型分析 被引量:1
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作者 周嘉青 陶璐 +5 位作者 颜克香 栾菁 张乔安 潘洁雯 张正华 沈蕾 《复旦学报(医学版)》 CAS CSCD 北大核心 2021年第3期337-342,共6页
目的比较斑块状寻常型银屑病(psoriasis vulgaris,PV)和汗孔角化症(porokeratosis,PK)患者的外周血Vγ9Vδ2 T细胞比例和表型的变化。方法采用流式细胞术来检测PV组(n=33)、PK组(n=19)及正常对照(normal control,NC)组(n=33)的外周血单... 目的比较斑块状寻常型银屑病(psoriasis vulgaris,PV)和汗孔角化症(porokeratosis,PK)患者的外周血Vγ9Vδ2 T细胞比例和表型的变化。方法采用流式细胞术来检测PV组(n=33)、PK组(n=19)及正常对照(normal control,NC)组(n=33)的外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中CD3、Vγ9、Vδ2、CLA、CD27、CD45RA的表达。结果与NC组相比较,Vγ9Vδ2 T细胞占总T细胞的比例和CLA+Vγ9Vδ2 T细胞占总Vγ9Vδ2 T细胞的比例分别在PV组(t=-2.349,P=0.0224;t=-3.277,P=0.0017)和PK组(t=-2.226,P=0.0306;t=-2.901,P=0.0055)出现显著减少;Vγ9Vδ2 T细胞分化成TCM的比例在PV组和PK组分别出现明显下降(t=-4.932,P<0.0001;t=-2.572,P=0.0131);同时,TEMRA的比例在PV组和PK组均出现明显升高(t=3.849,P=0.0003;t=2.236,P=0.0353);TEM比例的下降仅在PV组具有统计学意义(t=-3.303,P=0.0018)。结论PV和PK患者的外周血Vγ9Vδ2 T细胞的比例和表型具有相似性,该细胞可能迁移至皮肤,参与皮肤的自身炎症应答。 展开更多
关键词 寻常型银屑病(Pv) 汗孔角化症(PK) 92 t细胞
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Vγ9Vδ2 T细胞联合顺铂增强对鼻咽癌细胞的杀伤作用
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作者 苏凯 赵报 +5 位作者 马莹晔 李慧 周帅 王晓敏 张俊杰 马士崟 《蚌埠医学院学报》 CAS 2022年第11期1479-1483,共5页
目的:探讨Vγ9Vδ2 T免疫细胞联合顺铂(CDDP)对鼻咽癌细胞的杀伤作用。方法:采用CCK8法观察低浓度CDDP、唑来膦酸(ZOL)联合Vγ9Vδ2 T细胞对鼻咽癌细胞增殖活性的影响;流式细胞术检测Vγ9Vδ2 T细胞肿瘤坏死因子-α(TNF-α)、干扰素-γ(... 目的:探讨Vγ9Vδ2 T免疫细胞联合顺铂(CDDP)对鼻咽癌细胞的杀伤作用。方法:采用CCK8法观察低浓度CDDP、唑来膦酸(ZOL)联合Vγ9Vδ2 T细胞对鼻咽癌细胞增殖活性的影响;流式细胞术检测Vγ9Vδ2 T细胞肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)和CD107a的分泌及鼻咽癌细胞表面MICA和MICB的表达;Western blotting检测MICA和MICB的蛋白表达。结果:随着CDDP浓度的增加,鼻咽癌CNE2Z细胞存活率降低,CDDP联合Vγ9Vδ2 T细胞的杀伤作用优于单独使用CDDP对CNE2Z细胞的杀伤作用(P<0.05)。ZOL对Vγ9Vδ2 T细胞的增殖有促进作用,随着ZOL浓度提高,ZOL联合Vγ9Vδ2 T细胞对CNE2Z细胞的杀伤作用增强(P<0.05)。低浓度CDDP和ZOL联合Vγ9Vδ2 T细胞可增强对鼻咽癌细胞的杀伤作用,当效靶比为10∶1时,联合处理的杀伤效果最好,差异有统计学意义(P<0.05)。低浓度CDDP、ZOL处理的鼻咽癌细胞可增强Vγ9Vδ2 T细胞免疫因子CD107a、TNF-α和IFN-γ的分泌(P<0.05)。低浓度CDDP联合Vγ9Vδ2 T细胞明显上调鼻咽癌细胞表面MICA和MICB的表达(P<0.01)。结论:CDDP能增强Vγ9Vδ2 T细胞对鼻咽癌细胞的杀伤作用,其机制可能与促进鼻咽癌细胞表面MICA和MICB的表达及增加Vγ9Vδ2 T细胞TNF-α和IFN-γ的分泌有关。 展开更多
关键词 鼻咽肿瘤 92 t细胞 顺铂 免疫治疗
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免疫检查点T细胞激活抑制物免疫球蛋白可变区结构域、人内源性逆转录病毒-H长端重复相关蛋白2在子宫内膜癌中的表达及临床意义
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作者 柴静 张家弘 李梦雪 《中国性科学》 2024年第4期118-121,共4页
目的分析免疫检查点T细胞激活抑制物免疫球蛋白可变区结构域(VISTA)、人内源性逆转录病毒-H长端重复相关蛋白2(HHLA2)在子宫内膜癌(EC)中的表达及临床意义。方法选取2017年9月至2019年10月唐山市妇幼保健院收治的120例的EC患者作为研究... 目的分析免疫检查点T细胞激活抑制物免疫球蛋白可变区结构域(VISTA)、人内源性逆转录病毒-H长端重复相关蛋白2(HHLA2)在子宫内膜癌(EC)中的表达及临床意义。方法选取2017年9月至2019年10月唐山市妇幼保健院收治的120例的EC患者作为研究对象,收集其的EC组织和癌旁正常组织。检测EC组织和癌旁正常组织VISTA、HHLA2的表达;采用Spearman分析免疫检查点VISTA、HHLA2的相关性;Kaplan-Meier分析VISTA、HHLA2与预后的关系;Cox回归分析影响EC患者预后的危险因素。结果EC组织中VISTA、HHLA2阳性表达率显著高于正常癌旁组织(P<0.05)。Spearman相关性结果显示EC组织中VISTA与HHLA2表达呈正相关性(r=0.587,P<0.05)。EC组织中VISTA、HHLA2表达与临床病理特征有关(P<0.05)。Kaplan-Meier曲线结果显示VISTA阳性表达患者3年生存率低于阴性表达患者(χ^(2)=11.864,P<0.05),HHLA2阳性表达患者3年生存率低于阴性表达患者(χ^(2)=4.975,P<0.05)。Cox回归分析结果显示VISTA和HHLA2是影响EC患者预后的危险因素(P<0.05)。结论免疫检查点VISTA、HHLA2在EC中高表达,其是影响EC预后的危险因素,二者可能是有价值的预后标志物。 展开更多
关键词 t细胞激活抑制物免疫球蛋白可变区结构域 人内源性逆转录病毒-H长端重复相关蛋白2 子宫内膜癌 预后
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Cytotoxicity of Benzo(a)pyrene 7,8-dihydrodiol on Genetically Engineered V79 Chinese Hamster Cells Expressing Rat Liver Cytochrome P450 1A1
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作者 崔景荣 《Journal of Chinese Pharmaceutical Sciences》 CAS 1998年第3期35-39,共5页
To develop well defined in vitro cell system to test cytotoxicity of a number of model toxins, genetically engineered V79 Chinese hamster fibroblasts expressing isoenzymes of cytochrome P4501A1 XEM2 cells and V79 ce... To develop well defined in vitro cell system to test cytotoxicity of a number of model toxins, genetically engineered V79 Chinese hamster fibroblasts expressing isoenzymes of cytochrome P4501A1 XEM2 cells and V79 cells (parental), which lack cytochrome P450 enzyme activities, were used as controls. The cytotoxic effect of trans 7,8 dihydrbenzo(a)pyrene (BP 7,8 diol) on the parental cells V79 and V79 derived XEM2 cells were evaluated by two methods for cell viability. The data obtained expressed that BP 7,8 diol ranging from 1.0 μmol·L 1 to 5.0 μmol·L 1 in concentrations incubated for 24 h showed a strong cytotoxic effect in XEM2 cells (expressing rat cytochrome P4501A1) in a concentration dependent manner. Time dependent decrease for survival of XEM2 cells was also observed at 2.5 μmol·L 1 concentration. Likewise, BP 7,8 diol did not alter the survival of the parental cells V79 under the same condition. This study also showed that α naphthoflavone (αNF), a well known inhibitor of cytochrome P4501A1 might alter BP 7,8 diol induced cytotoxicity in the XEM2 cells. Our results suggested that cytochrome P4501A1 is responsible for BP 7, 8 diol induced cytotoxicity. 展开更多
关键词 v79 cell line XEM2 cell line BP 7 8 diol Cytotoxicity P4501A1
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Correlation of matrix metalloproteinase-2, -9, tissue inhibitor-1 of matrix metalloproteinase and CD44 variant 6 in head and neck cancer metastasis 被引量:8
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作者 徐娅苹 赵学群 +1 位作者 SOMMER,K. MOUBAYED,P. 《Journal of Zhejiang University Science》 CSCD 2003年第4期491-501,共11页
This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase 2, 9 (MMP 2, MMP 9), tissue inhibitor 1 of matrix metalloproteinase (TIMP 1), c... This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase 2, 9 (MMP 2, MMP 9), tissue inhibitor 1 of matrix metalloproteinase (TIMP 1), cell adhesion molecule 44 variant 6 (CD44v6), HER2/neu and p53 was investigated in 154 patients with head and neck squamous cell carcinoma (SCC) by ABC and ImmunoMax immunohistochemical method. Their clinical relevance and correlation were analysed. The expression of MMP 2, MMP 9, TIMP 1, CD44v6, HER2/neu and p53 was found in cancer cells in 87.01%, 85.71%, 68.18%, 98.05%, 55.19% and 50.65% cases respectively. Linear regression and correlation analysis revealed that there was close positive relationship ( P <0.05) between the expression of MMP 2 and MMP 9, TIMP 1 and CD44v6, HER2/neu and MMP 9, MMP 2 and p53. Up regulation of MMP 2 was accompanied by advanced T stage ( P <0.01) . There was also a trend of MMP 2 expression being related with tumor metastasis. Increased expression of HER2/neu was found in patients with tumor recurrence( P <0.05). The expression of TIMP 1 was higher in laryngeal cancer than that in pharyngeal cancer, and higher in keratinizing and non keratinizing SCC than that in basaloid SCC( P <0.05). These findings suggested that MMP 2 and MMP 9, HER2/neu and MMP 9, MMP 2 and p53 had a coordinate function in aggression of tumor; that MMP 2 had a more important function than MMP 9 in tumor invasion and metastasis; and that HER2/neu might serve as a biomarker for poor prognosis in HNSCC. 展开更多
关键词 Head and neck cancer Matrix metalloproteinase 2 9 (MMP 2 and MMP 9) tissue inhibitor 1 of matrix metalloproteinase (tIMP 1) cell adhesion molecule 44 variant 6 (CD44 v6) HER2/NEU p53
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