This study was conducted to identify a cheap and suitable culture medium for the mycelial growth and sporulation of Sternphylium vesicarium and to determine the cultural and morphological variability of this pathogen....This study was conducted to identify a cheap and suitable culture medium for the mycelial growth and sporulation of Sternphylium vesicarium and to determine the cultural and morphological variability of this pathogen. A total of 24 isolates of S. vesicarium collected from eight different onion growing areas were characterized in terms of cultural and morphological aspects. Front colony colors were greenish brown to dirty white, deep grey to whitish, light grey to whitish, deep greenish white, light grey and dirty white to greenish. Reverse colony colors were brown, deep brown and light brown. Colony shapes were circular and irregular with umbonate, raised and flat type colony elevation. Colony textures were cottony, fluffy and velvety with entire, undulate and filiform type colony margin. Among the culture media, V-7 juice agar found to be the most suitable culture media for mycclial growth of S. vesicarium. The sporulation of the fungus was remarkably influenced by V-7 juice mixed with potato dextrose agar （PDA） media, this media exhibited the highest sporulation （87.76-169.0/mm^2） of S. vesicariumin comparison with other media. The minimum days （28 d to 31 d） for conidial production were observed on V-7 juice agar medium. The length of conidia varied from 14.6 μm to 30.6 μm. The maximum mean length of conidia was 29.97 μm found in isolate DSSA, while the minimum mean length 17.36 μm was found in isolate MSMM 02. The breadth of conidia ranged from 4.7 μm to 15.7μm. The maximum mean breadth of conidia was 12.55 μm found in the isolate DSSA, while the minimum mean breadth 9.760 μm was found in the isolate CCKH 02. The horizontal septation varied from l to 3 and the longitudinal septation varied from 0 to 4.展开更多
Objective. The goal of this paper is to investigate the relationship between the N-glycosylation of acetylglucosaminyltransferase V(Glc NAcT-V) and its activity and to know which site among the 6 N-glycosylation sites...Objective. The goal of this paper is to investigate the relationship between the N-glycosylation of acetylglucosaminyltransferase V(Glc NAcT-V) and its activity and to know which site among the 6 N-glycosylation sites in the GlcNAcT-V gene is the most important. Methods.Wild type of GlcNAcTV was transfected into COS7 cells and its activity was measured 48 h later. The first site (Asn 110) was mutated with sitedirected mutagenesis and transfected into COS7 cells. Results. It was found that after the cells were added tunicamycin(TM, 1 μ g/ml), the activity was 117% of the wild type. The activity of the cells with mutating GlcNAcTV was about 120% of the wild type RTPCR showed that there was no significant change in mRNA expression among the three groups. Conclusion.The Nglycosylation is important for its activity. Our results suggest that the Nlinked carbohydrates on GlcNAcTV are required for the posttranscriptional activity of the enzyme.展开更多
文摘This study was conducted to identify a cheap and suitable culture medium for the mycelial growth and sporulation of Sternphylium vesicarium and to determine the cultural and morphological variability of this pathogen. A total of 24 isolates of S. vesicarium collected from eight different onion growing areas were characterized in terms of cultural and morphological aspects. Front colony colors were greenish brown to dirty white, deep grey to whitish, light grey to whitish, deep greenish white, light grey and dirty white to greenish. Reverse colony colors were brown, deep brown and light brown. Colony shapes were circular and irregular with umbonate, raised and flat type colony elevation. Colony textures were cottony, fluffy and velvety with entire, undulate and filiform type colony margin. Among the culture media, V-7 juice agar found to be the most suitable culture media for mycclial growth of S. vesicarium. The sporulation of the fungus was remarkably influenced by V-7 juice mixed with potato dextrose agar （PDA） media, this media exhibited the highest sporulation （87.76-169.0/mm^2） of S. vesicariumin comparison with other media. The minimum days （28 d to 31 d） for conidial production were observed on V-7 juice agar medium. The length of conidia varied from 14.6 μm to 30.6 μm. The maximum mean length of conidia was 29.97 μm found in isolate DSSA, while the minimum mean length 17.36 μm was found in isolate MSMM 02. The breadth of conidia ranged from 4.7 μm to 15.7μm. The maximum mean breadth of conidia was 12.55 μm found in the isolate DSSA, while the minimum mean breadth 9.760 μm was found in the isolate CCKH 02. The horizontal septation varied from l to 3 and the longitudinal septation varied from 0 to 4.
文摘Objective. The goal of this paper is to investigate the relationship between the N-glycosylation of acetylglucosaminyltransferase V(Glc NAcT-V) and its activity and to know which site among the 6 N-glycosylation sites in the GlcNAcT-V gene is the most important. Methods.Wild type of GlcNAcTV was transfected into COS7 cells and its activity was measured 48 h later. The first site (Asn 110) was mutated with sitedirected mutagenesis and transfected into COS7 cells. Results. It was found that after the cells were added tunicamycin(TM, 1 μ g/ml), the activity was 117% of the wild type. The activity of the cells with mutating GlcNAcTV was about 120% of the wild type RTPCR showed that there was no significant change in mRNA expression among the three groups. Conclusion.The Nglycosylation is important for its activity. Our results suggest that the Nlinked carbohydrates on GlcNAcTV are required for the posttranscriptional activity of the enzyme.
