Tilia amurensis is an economically valuable broadleaf tree species in Northeast China.The production of highqualityT.amurensis varieties at commercial scales has been greatly limited by the low germination rates.There...Tilia amurensis is an economically valuable broadleaf tree species in Northeast China.The production of highqualityT.amurensis varieties at commercial scales has been greatly limited by the low germination rates.Thereis thus a pressing need to develop an organogenesis protocol for in vitro propagation of T.amurensis to alleviate ashortage of high-quality T.amurensis seedlings.Here,we established a rapid in vitro propagation system forT.amurensis from mature zygotic embryos and analyzed the effects of plant growth regulators and culture mediain different stages.We found that Woody plant medium(WPM)was the optimal primary culture medium formature zygotic embryos.The highest callus induction percentage(68.76%)and number of axillary buds induced(3.2)were obtained in WPM+0.89μmol/L 6-benzyladenine(6-BA)+0.46μmol/L kinetin(KT)+0.25μmol/Lindole-3-butryic acid(IBA)+1.44μmol/L gibberellin A_(3)(GA_(3)).The multiple shoot bud development achievedthe highest percentage(83.32%)in the Murashige and Skoog(MS)+2.22μmol/L 6-BA+0.25μmol/L IBA+1.44μmol/L GA_(3).The rooting percentage(96.70%)was highest in 1/2 MS medium+1.48μmol/L IBA.Thesurvival percentage of transplanting plantlets was 82.22%in soil:vermiculite:perlite(5:3:1).Our study is the firstto establish an effective organogenesis protocol for T.amurensis using mature zygotic embryos.展开更多
[ Objective] This study aimed to explore the genetic diversity of Vitis amurensis Rupr. （Vitaceae） germplasm resources. [ Method ] Out of total 245 pairs of primers, 18 were selected for SSR amplification of 360 V. ...[ Objective] This study aimed to explore the genetic diversity of Vitis amurensis Rupr. （Vitaceae） germplasm resources. [ Method ] Out of total 245 pairs of primers, 18 were selected for SSR amplification of 360 V. amurensis experimental materials. [Result] The number of bands amplified by each primer ranged from 4 to 13 with a mean of 9.44. The length of bands ranged from 150 to 1 000 bp, concentrated at 200 -750 bp. The 18 pairs of primers amplified 170 bands totally, of which 167 bands were polymorphic with a polymorphism ratio of 98.2%. The Shannon＇s diversity index （I） is 1. 778 051. With the SSR-PCR am- plification of 360 V. amurensis varieties （ strains）, 5 specific bands were amplified by certain primers in several varieties（strains） accounting for 2.95% of the total bands. [ Conclusion] SSR molecular marker technique was an efficient method to detect the genetic diversity of V. amurensis and thereby is an effective tool for pedigree analysis and variety identification of A. amurensis varieties（strains）.展开更多
The deciduous linden tree （Tilia amurensis Rupr.） is protected at National Level II in China as a species of ecological and economic importance. The objective of this study was to assess and compare the ectomycorrhi...The deciduous linden tree （Tilia amurensis Rupr.） is protected at National Level II in China as a species of ecological and economic importance. The objective of this study was to assess and compare the ectomycorrhizal communities associated with T. amurensis in natural versus urban forests of central Heilongjiang Province. The percentage of T. amurensis colonisation by ectomycorrhiza was more than 60 % in urban forests, compared to 34-49 % in natural forests. Use of a combi- nation of morphological and molecular methods docu- mented 18 ECM （ectomycorrhizal） types among three sites; 12-13 ECM species were identified in the natural sites versus 9 species in the urban site. Four ECM species （Boletus sp., Tuber sp., Inocybe sp.2, Leccinum sp.1） were the dominant mycorrhizal symbionts, and Cenococcum geophilum and Russula sp. were found only in the natural forests.展开更多
基金This work was supported by the Science and Technology Development Plan Project of Jilin Province,China(20200402115NC).
文摘Tilia amurensis is an economically valuable broadleaf tree species in Northeast China.The production of highqualityT.amurensis varieties at commercial scales has been greatly limited by the low germination rates.Thereis thus a pressing need to develop an organogenesis protocol for in vitro propagation of T.amurensis to alleviate ashortage of high-quality T.amurensis seedlings.Here,we established a rapid in vitro propagation system forT.amurensis from mature zygotic embryos and analyzed the effects of plant growth regulators and culture mediain different stages.We found that Woody plant medium(WPM)was the optimal primary culture medium formature zygotic embryos.The highest callus induction percentage(68.76%)and number of axillary buds induced(3.2)were obtained in WPM+0.89μmol/L 6-benzyladenine(6-BA)+0.46μmol/L kinetin(KT)+0.25μmol/Lindole-3-butryic acid(IBA)+1.44μmol/L gibberellin A_(3)(GA_(3)).The multiple shoot bud development achievedthe highest percentage(83.32%)in the Murashige and Skoog(MS)+2.22μmol/L 6-BA+0.25μmol/L IBA+1.44μmol/L GA_(3).The rooting percentage(96.70%)was highest in 1/2 MS medium+1.48μmol/L IBA.Thesurvival percentage of transplanting plantlets was 82.22%in soil:vermiculite:perlite(5:3:1).Our study is the firstto establish an effective organogenesis protocol for T.amurensis using mature zygotic embryos.
基金Supported by Special Fund for the Industrial Technology System Construction of Modern Agriculture(nycytx-30)
文摘[ Objective] This study aimed to explore the genetic diversity of Vitis amurensis Rupr. （Vitaceae） germplasm resources. [ Method ] Out of total 245 pairs of primers, 18 were selected for SSR amplification of 360 V. amurensis experimental materials. [Result] The number of bands amplified by each primer ranged from 4 to 13 with a mean of 9.44. The length of bands ranged from 150 to 1 000 bp, concentrated at 200 -750 bp. The 18 pairs of primers amplified 170 bands totally, of which 167 bands were polymorphic with a polymorphism ratio of 98.2%. The Shannon＇s diversity index （I） is 1. 778 051. With the SSR-PCR am- plification of 360 V. amurensis varieties （ strains）, 5 specific bands were amplified by certain primers in several varieties（strains） accounting for 2.95% of the total bands. [ Conclusion] SSR molecular marker technique was an efficient method to detect the genetic diversity of V. amurensis and thereby is an effective tool for pedigree analysis and variety identification of A. amurensis varieties（strains）.
基金financially supported by Wild Plants Protection Management Program of State Forestry Administration and Start Research Grants of Postdoctoral Researcher in Heilongjiang
文摘The deciduous linden tree （Tilia amurensis Rupr.） is protected at National Level II in China as a species of ecological and economic importance. The objective of this study was to assess and compare the ectomycorrhizal communities associated with T. amurensis in natural versus urban forests of central Heilongjiang Province. The percentage of T. amurensis colonisation by ectomycorrhiza was more than 60 % in urban forests, compared to 34-49 % in natural forests. Use of a combi- nation of morphological and molecular methods docu- mented 18 ECM （ectomycorrhizal） types among three sites; 12-13 ECM species were identified in the natural sites versus 9 species in the urban site. Four ECM species （Boletus sp., Tuber sp., Inocybe sp.2, Leccinum sp.1） were the dominant mycorrhizal symbionts, and Cenococcum geophilum and Russula sp. were found only in the natural forests.
