THE STRUCTURES OF YIYELIANGWANOSIDE Ⅶ AND Ⅷ FROM THE BARK OF NOTHOPANAX DA VIDII(FRANCH)HARMS

Twonew oleanane type saponins wereisolated from the alcoholic extract of the bark of Nothopanax davidii(Franch)Harms(Araliaceae).Their structures have been determined on th e basis of spectral and chemical data as 3-O-β-(2',4'-O-diacetyl)-D-xylopyranosyl-3β-hydroxyolean-12-ene- 28,29-dioic acid-28-O-[α-L-rhamnopyranosyl(1-4)-β-D-glucopyranosyl(1-6)-β-D-glucopyranosyl] ester(1),named yiyeliangwanoside Ⅷ;3-O-β-(3'-O-acetyl)-D-xylopyranosyl-3β-hydroxyolean-12- ene-28,29-dioic acid-28-O-[O-L-rhamnopyranosyl(1-4)-β-D-glucopyranosyl(1-6)-β-D- glucopyranosyl] ester(Ⅱ),named yiyeliangwanoside Ⅷ.

不同处理对刺葡萄愈伤组织花青素和原花青素生物合成的影响

以刺葡萄2个同质不同型的愈伤组织细胞系为材料,研究不同处理因子对其花青素和原花青素合成能力的影响。试验结果表明,刺葡萄DLR细胞系具有高产花青素和原花青素的能力,在培养至45 d时,其花青素与原花青素的含量均达到最高值,分别为113.85μg/g(FW)和3 562.95μg/g(FW);低温、高温、肉桂酸、壳聚糖、紫外线、黑暗和KT等因子,对DLR细胞系花青素和原花青素的累积表现出不同的效应;其中,4℃低温处理下,DLR细胞花青素的积累效果最佳,比对照提高了2.14倍;壳聚糖处理下,原花青素积累效果最佳,是对照的2.84倍。刺葡萄DLW细胞系不具有或极低的花青素和原花青素合成能力,处理因子调控对其作用效果不明显。本研究的结论为进一步进行刺葡萄细胞花青素和原花青素合成调控奠定了基础。