Abstract： Vanadium compounds are promising therapeutic agents for the treatment of diabetes and cancer. However, vanadium toxicity has been a great concern. Many works suggested that vanadium-induced oxidative stress...Abstract： Vanadium compounds are promising therapeutic agents for the treatment of diabetes and cancer. However, vanadium toxicity has been a great concern. Many works suggested that vanadium-induced oxidative stress is a major reason of vanadium toxicity. Quercetin and green tea polyphenols （GTP） are well-known antioxidants that play important roles in the prevention of many oxidative stress-related diseases. In this study, we investigated the protective effects of quercetin and GTP against damages caused by VO（acac）2 on isolated mitochondria and whole cells. The experimental results demonstrated that quercetin and GTP could significantly inhibit mitochondrial dysfimctions induced by VO（acac）2, such as mitochondrial swelling, depolarization of the inner mitochondrial membrane potential （△ψm）, and increased release of reactive oxygen species （ROS）. Moreover, they also reduce cytotoxicity in Hep G2 cells. These results indicated that use of natural antioxidants to control the metal toxicity of vanadium compounds may be a promising strategy for developing metal-based therapeutic agents.展开更多
The catalytic activity of VO（acac）2 for the cyclohexane（Cy） oxidation was studied. The effects of various parameters, such as the amounts of H202, HNO3, H20 and Cy were investigated. The highest total turnover num...The catalytic activity of VO（acac）2 for the cyclohexane（Cy） oxidation was studied. The effects of various parameters, such as the amounts of H202, HNO3, H20 and Cy were investigated. The highest total turnover num- ber（TON） is 234, which can be increased to 353, 342 and 403, respectively with the adding of o-phthalic, m-phthalic or p-phthalic acid. A reaction mechanism was also supposed primarily.展开更多
epi-Chrysotricine 6 was synthesized by a cascade stereoselective oxidative cyclization of 5-hydroxyalkene 3 using tert-butyl hydroperoxide(TBHP) catalyzed by VO(acac)2.
In this study, we aimed to clarify the source of the reactive oxygen species （ROS） generation induced by vanadium compounds. We used vanadyl acetylacetonate （VO（acac）2）, a highly effective agent in controlling h...In this study, we aimed to clarify the source of the reactive oxygen species （ROS） generation induced by vanadium compounds. We used vanadyl acetylacetonate （VO（acac）2）, a highly effective agent in controlling hyperglycemia, to determine the source of ROS generation in two renal cell lines LLC-PK1 and MDCK. Four commonly fluorescent dyes were used to assess VO（acac）2-induced H202 and ＂02 production and their location. It demonstrated that VO（acac）2 can induce significant ROS generation in both LLC-PKI and MDCK cells, which were primarily derived from mitochondria. The results obtained in this study raised the possibility to reduce ROS level induced by vanadium compounds locally and thus avoid affecting its activity.展开更多
基金National Natural Science Foundation of China (Grant No.20671008).
文摘Abstract： Vanadium compounds are promising therapeutic agents for the treatment of diabetes and cancer. However, vanadium toxicity has been a great concern. Many works suggested that vanadium-induced oxidative stress is a major reason of vanadium toxicity. Quercetin and green tea polyphenols （GTP） are well-known antioxidants that play important roles in the prevention of many oxidative stress-related diseases. In this study, we investigated the protective effects of quercetin and GTP against damages caused by VO（acac）2 on isolated mitochondria and whole cells. The experimental results demonstrated that quercetin and GTP could significantly inhibit mitochondrial dysfimctions induced by VO（acac）2, such as mitochondrial swelling, depolarization of the inner mitochondrial membrane potential （△ψm）, and increased release of reactive oxygen species （ROS）. Moreover, they also reduce cytotoxicity in Hep G2 cells. These results indicated that use of natural antioxidants to control the metal toxicity of vanadium compounds may be a promising strategy for developing metal-based therapeutic agents.
基金Supported by the National Natural Science Foundation of China（Nos.21306073, 21371086） and the Science and Technology Fund for Outstanding Young Talents in Dalian, China（No.2012J21DW007）.
文摘The catalytic activity of VO（acac）2 for the cyclohexane（Cy） oxidation was studied. The effects of various parameters, such as the amounts of H202, HNO3, H20 and Cy were investigated. The highest total turnover num- ber（TON） is 234, which can be increased to 353, 342 and 403, respectively with the adding of o-phthalic, m-phthalic or p-phthalic acid. A reaction mechanism was also supposed primarily.
基金the National Research Center for Analysis of Drugs and Metabolites for recording NMR and MS data. This project was partially fo
文摘epi-Chrysotricine 6 was synthesized by a cascade stereoselective oxidative cyclization of 5-hydroxyalkene 3 using tert-butyl hydroperoxide(TBHP) catalyzed by VO(acac)2.
基金National Natural Science Foundation of China(Grant No. 20871008 and 21171011)
文摘In this study, we aimed to clarify the source of the reactive oxygen species （ROS） generation induced by vanadium compounds. We used vanadyl acetylacetonate （VO（acac）2）, a highly effective agent in controlling hyperglycemia, to determine the source of ROS generation in two renal cell lines LLC-PK1 and MDCK. Four commonly fluorescent dyes were used to assess VO（acac）2-induced H202 and ＂02 production and their location. It demonstrated that VO（acac）2 can induce significant ROS generation in both LLC-PKI and MDCK cells, which were primarily derived from mitochondria. The results obtained in this study raised the possibility to reduce ROS level induced by vanadium compounds locally and thus avoid affecting its activity.
