Varicella (chickenpox) is a generalized, self-limiting viral infection that is caused by varicella zoster virus (VZV). Chickenpox commonly infects children from 2 - 8 years without severe outcomes, but is particularly...Varicella (chickenpox) is a generalized, self-limiting viral infection that is caused by varicella zoster virus (VZV). Chickenpox commonly infects children from 2 - 8 years without severe outcomes, but is particularly severe when affecting adults.展开更多
Methylene blue injection has a stronger direct inactivation on VZV in vitro. When the injection was diluted from 116 to 1128, which was obvious (P<001 and P<005). The MIC was 1222, the IC50 was 1135 and IC90 was...Methylene blue injection has a stronger direct inactivation on VZV in vitro. When the injection was diluted from 116 to 1128, which was obvious (P<001 and P<005). The MIC was 1222, the IC50 was 1135 and IC90 was 177. The results of microcellculture method showed when the injection was diluted from 116 to 164, it also effectively inhibited the proliferation of VZV in WISH continuous celllines (P<001 and P<005). The MIC was 195, the IC50 was 145 and the IC90 was 121.展开更多
Latent varicella-zoster virus(VZV)may be reactivated to cause herpes zoster,which affects one in three people during their lifetime.The currently available subunit vaccine Shingrix^(TM) is superior to the attenuated v...Latent varicella-zoster virus(VZV)may be reactivated to cause herpes zoster,which affects one in three people during their lifetime.The currently available subunit vaccine Shingrix^(TM) is superior to the attenuated vaccine Zostavax®in terms of both safety and efficacy,but the supply of its key adjuvant component QS21 is limited.With ionizable lipid nanoparticles(LNPs)that were recently approved by the FDA for COVID-19 mRNA vaccines as carriers,and oligodeoxynucleotides containing CpG motifs(CpG ODNs)approved by the FDA for a subunit hepatitis B vaccine as immunostimulators,we developed a LNP vaccine encapsulating VZV-glycoprotein E(gE)and CpG ODN,and compared its immunogenicity with Shingrix^(TM) in C57BL/6J mice.The results showed that the LNP vaccine induced comparable levels of gE-specific IgG antibodies to Shingrix^(TM) as determined by enzymelinked immunosorbent assay(ELISA).Most importantly,the LNP vaccine induced comparable levels of cellmediated immunity(CMI)that plays decisive roles in the efficacy of zoster vaccines to Shingrix^(TM) in a VZVprimed mouse model that was adopted for preclinical studies of Shingrix^(TM) .Number of IL-2 and IFN-γsecreting splenocytes and proportion of T helper 1(Th1)cytokine-expressing CD4^(+)T cells in LNP-CpG-adjuvanted VZV-gE vaccinated mice were similar to that of Shingrix^(TM) boosted mice.All of the components in this LNP vaccine can be artificially and economically synthesized in large quantities,indicating the potential of LNP-CpGadjuvanted VZV-gE as a more cost-effective zoster vaccine.展开更多
文摘Varicella (chickenpox) is a generalized, self-limiting viral infection that is caused by varicella zoster virus (VZV). Chickenpox commonly infects children from 2 - 8 years without severe outcomes, but is particularly severe when affecting adults.
文摘Methylene blue injection has a stronger direct inactivation on VZV in vitro. When the injection was diluted from 116 to 1128, which was obvious (P<001 and P<005). The MIC was 1222, the IC50 was 1135 and IC90 was 177. The results of microcellculture method showed when the injection was diluted from 116 to 164, it also effectively inhibited the proliferation of VZV in WISH continuous celllines (P<001 and P<005). The MIC was 195, the IC50 was 145 and the IC90 was 121.
基金supported by the Major Science and Technology Special Projects of Yunnan Province,China (202002AA100009)the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences (2021-JKCS-012)+5 种基金the Special Biomedicine Projects of Yunnan Province (202102AA310035)National Natural Science Foundation of China (82104130)Fundamental Research Funds for the Central Universities (3332021072)the Basic Research Projects of Yunnan Province (202101AU070176, 202101AT070286)the Funds for the Training of High-level Health Technical Personnel in Yunnan Province(grant number H-2019063)the Funds for High-level Scientific and Technological Talents Selection Special Project of Yunnan Province(202205AC160015)
文摘Latent varicella-zoster virus(VZV)may be reactivated to cause herpes zoster,which affects one in three people during their lifetime.The currently available subunit vaccine Shingrix^(TM) is superior to the attenuated vaccine Zostavax®in terms of both safety and efficacy,but the supply of its key adjuvant component QS21 is limited.With ionizable lipid nanoparticles(LNPs)that were recently approved by the FDA for COVID-19 mRNA vaccines as carriers,and oligodeoxynucleotides containing CpG motifs(CpG ODNs)approved by the FDA for a subunit hepatitis B vaccine as immunostimulators,we developed a LNP vaccine encapsulating VZV-glycoprotein E(gE)and CpG ODN,and compared its immunogenicity with Shingrix^(TM) in C57BL/6J mice.The results showed that the LNP vaccine induced comparable levels of gE-specific IgG antibodies to Shingrix^(TM) as determined by enzymelinked immunosorbent assay(ELISA).Most importantly,the LNP vaccine induced comparable levels of cellmediated immunity(CMI)that plays decisive roles in the efficacy of zoster vaccines to Shingrix^(TM) in a VZVprimed mouse model that was adopted for preclinical studies of Shingrix^(TM) .Number of IL-2 and IFN-γsecreting splenocytes and proportion of T helper 1(Th1)cytokine-expressing CD4^(+)T cells in LNP-CpG-adjuvanted VZV-gE vaccinated mice were similar to that of Shingrix^(TM) boosted mice.All of the components in this LNP vaccine can be artificially and economically synthesized in large quantities,indicating the potential of LNP-CpGadjuvanted VZV-gE as a more cost-effective zoster vaccine.
