A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue...A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO2 enrichment, sucrose-reduced medium, and light intensity of 60 mM m-2·s-1. Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adaptability and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a photomixotrophic stage in the vitroplantlets cultured in TIBs.展开更多
AIM: To study isolation and chemical characterization of pectin derived from the common cranberry Vaccinium oxycoccos L. (oxycoccusan OP) and the testing of its preventive effect on experimental colitis.METHODS: Mice ...AIM: To study isolation and chemical characterization of pectin derived from the common cranberry Vaccinium oxycoccos L. (oxycoccusan OP) and the testing of its preventive effect on experimental colitis.METHODS: Mice were administrated orally with OP two days prior to a rectal injection of 5% acetic acid and examined for colonic damage 24 h later. Colonic inflammation was characterized by macroscopical injury and enhanced levels of myeloperoxidase activity measured spectrophotometrically with o-phenylene diamine as the substrate. The mucus contents of the colon were determined by the Alcian blue dye binding method. Vascular permeability was estimated using 4% Evans blue passage after i.p. injection of 0.05 mol/L acetic acid. RESULTS: In the mice treated with OP, colonic macroscopic scores (1.1 ± 0.4 vs 2.7, P < 0.01) and the total square area of damage (10 ± 2 vs 21 ± 7, P < 0.01) were significantly reduced when compared with the vehicle-treated colitis group. OP was shown to decrease the tissue myeloperoxidase activity in colons (42 ± 11 vs 112 ± 40, P < 0.01) and enhance the amount of mucus of colitis mice (0.9 ± 0.1 vs 0.4 ± 0.1, P < 0.01). The level of colonic malondialdehyde was noted to decrease in OP-pretreated mice (3.6 ± 0.7 vs 5.1 ± 0.8, P < 0.01). OP was found to decrease the inflammatory status of mice as was determined by reduction of vascular permeability (161 ± 34 vs 241 ± 21, P < 0.01). Adhesionof peritoneal neutrophils and macrophages was also shown to decrease after administration of OP (141 ± 50 vs 235 ± 37, P < 0.05).CONCLUSION: Thus, a preventive effect of pectin from the common cranberry, namely oxycoccusan OP, on acetic acid-induced colitis in mice was detected. A reduction of neutrophil infiltration and antioxidant action may be implicated in the protective effect of oxycoccusan.展开更多
The swinging type temporary immersion bioreactors system( S-TTBs) is a kind of new and advanced method of tissue culture. The efficient multiplication technology of blueberry( Vaccinium corymbosum) plantlets in S-TTBs...The swinging type temporary immersion bioreactors system( S-TTBs) is a kind of new and advanced method of tissue culture. The efficient multiplication technology of blueberry( Vaccinium corymbosum) plantlets in S-TTBs was systematically studied through L_9( 3~4) orthogonal experiment with three factors( culture medium volume,swing angle,and inoculation density) and completely randomized experiment with two factors( swing frequency and immersion time).The results showed that the optimal culture parameters were set as follows: the culture medium volume of 250 ml/bottle,the swing angle at 45°,and the inoculation density of 60 plantlets/bottle. The optimal swing frequency was 1 time/6 h,and the immersion time was set as 60 s.展开更多
Aims Ericaceous plant species can host diverse fungi in their roots,including ericoid mycorrhizal fungi(ERMF),endophytes,pathogens and some species with unknown functions.However,how this diversity of fungi responds t...Aims Ericaceous plant species can host diverse fungi in their roots,including ericoid mycorrhizal fungi(ERMF),endophytes,pathogens and some species with unknown functions.However,how this diversity of fungi responds to different human disturbances is not well understood.Methods In this study,we examined the effects of different human disturbance on fungal diversity in hair roots of Vaccinium mandarinorum,an ericaceous plant.Fungal DNA was extracted from hair roots of V.mandarinorum and high-throughput sequencing was applied to detect the diversity of root-associated fungi along a human disturbance gradient in subtropical forests in Gutianshan National Nature Reserve(GNNR)in East China.The four forest types with different disturbance regime were:old growth forest(OGF),secondary forest with once cut(SEC I),secondary forest with twice cut(SEC II)and Cunninghamia lanceolata plantation(PLF).Important Findings The results showed that:(i)diverse fungal operational units(OTUs)were detected in hair roots of V.mandarinorum in the four types of forests,covering fungal phyla of Ascomycota,Basidiomycota,Chytridiomycota,Glomeromycota and Zygomycota;(ii)Community composition of root-associated fungi of V.mandarinorum in PLF was distinct from those in the other three forest types,and two types of secondary forests had similar fungal community composition;(iii)Different fungal families respond differently to human disturbances:fungal families with significant preference to OGF were ectomycorrhizal or saprophytic fungi while fungal families with higher relative abundance in PLF were plant pathogenic or saprophytic fungi;(iv)The first principal component(PC1)of plant community had a significant effect on composition of root-associated fungal community,while edaphic parameters showed no significant effect on fungal community composition in roots of V.mandarinorum.Our results help to better understand the responses of both ericaceous plants and their fungal partners to human disturbances and forest managements.展开更多
Vaccinium darrowii is a subtropical wild blueberry species that has been used to breed economically important southern highbush cultivars.The adaptive traits of V.darrowii to subtropical climates can provide valuable ...Vaccinium darrowii is a subtropical wild blueberry species that has been used to breed economically important southern highbush cultivars.The adaptive traits of V.darrowii to subtropical climates can provide valuable information for breeding blueberry and perhaps other plants,especially against the background of global warming.Here,we assembled the V.darrowii genome into 12 pseudochromosomes using Oxford Nanopore long reads complemented with Hi-C scaffolding technologies,and we predicted 41815 genes using RNA-sequencing evidence.Syntenic analysis across three Vaccinium species revealed a highly conserved genome structure,with the highest collinearity between V.darrowii and Vaccinium corymbosum.This conserved genome structure may explain the high fertility observed during crossbreeding of V.darrowii with other blueberry cultivars.Analysis of gene expansion and tandem duplication indicated possible roles for defense-and flowering-associated genes in the adaptation of V.darrowii to the subtropics.Putative SOC1 genes in V.darrowii were identified based on phylogeny and expression analysis.Blueberries are covered in a thick cuticle layer and contain anthocyanins,which confer their powdery blue color.Using RNA sequencing,we delineated the cuticle biosynthesis pathways of Vaccinium species in V.darrowii.This result can serve as a reference for breeding berries whose colors are appealing to customers.The V.darrowii reference genome,together with the unique traits of this species,including its diploid genome,short vegetative phase,and high compatibility in hybridization with other blueberries,make V.darrowii a potential research model for blueberry species.展开更多
Objective: To study the protective effect of anthocyanins extracted from Vaccinium Uliginosum(VU)on retinal 661W cells against microwave radiation induced retinal injury. Methods: 661W cells were divided into 6 groups...Objective: To study the protective effect of anthocyanins extracted from Vaccinium Uliginosum(VU)on retinal 661W cells against microwave radiation induced retinal injury. Methods: 661W cells were divided into 6 groups, including control, model [661W cells radiated by microwave(30 mW/cm2, 1 h)] and VU groups [661W cells pretreated with anthocyanins extracted from VU(25, 50, 100 and 200 μg/mL, respectively) for 48 h, and radiated by microwave 30 mW/cm2, 1 h]. After treatment with different interventions, the cell apoptosis index(AI)was determined using Heochst staining;contents of malonaldehyde(MDA), glutataione(GSH), and activity of superoxide dismutase(SOD) were measured. mRNA expressions of nuclear factor erythroid 2-related factor 2(Nrf2) and heme oxygenase 1(HO-1) were detected by real time quantitative polymerase chain reaction, and the expression of HO-1 protein was examined by Western blot analysis. Nucleus and cytoplasm were separated and Nrf2 protein expression was further verified by Western blot analysis. Results: There was significant difference in AI among the groups(F=322.83, P<0.05). Compared with the control group, AI was significantly higher in the model group and was lower in 4 VU-pretreated groups(P<0.05). Linear regression analysis showed the decline of AI was in a dose-dependent manner with VU treatment(r=0.8419, P<0.05). The MDA and GSH contents of 661W cells in VU-treated groups were significantly lower than the model group(P<0.05). Compared with the model group, the SOD activity in the VU-treated groups(50, 100 and 200 μg/mL) was significantly higher(all P<0.05). The Nrf2 and HO-1 mRNA expressions were slightly increased after irradiation, and obviously increased in 100 μg/mL VU-treated group. After irradiation, the relative expressions of HO-1 and Nrf2 proteins in nucleus were slightly increased(P<0.05), and the changes in cytoplasm were not obvious,whereas it was significantly increased in both nucleus and cytoplasm in the VU treatment groups. Conclusions:Anthocyanins extracted from VU could reduce apoptosis, stabilize cell membrane, and alleviate oxidant injury of mouse retinal photoreceptor 661W cells. The mechanism might be through activating Nrf2/HO-1 signal pathway and inducing HO-1 transcription and translation.展开更多
文摘A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO2 enrichment, sucrose-reduced medium, and light intensity of 60 mM m-2·s-1. Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adaptability and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a photomixotrophic stage in the vitroplantlets cultured in TIBs.
基金the Program of Presidium of the Russian Acad.Sci. <>, the Russian Fund forBasic Research, No. 06-04-48079 the Program for LeadingScientific Schools, No. 5796.2006.4
文摘AIM: To study isolation and chemical characterization of pectin derived from the common cranberry Vaccinium oxycoccos L. (oxycoccusan OP) and the testing of its preventive effect on experimental colitis.METHODS: Mice were administrated orally with OP two days prior to a rectal injection of 5% acetic acid and examined for colonic damage 24 h later. Colonic inflammation was characterized by macroscopical injury and enhanced levels of myeloperoxidase activity measured spectrophotometrically with o-phenylene diamine as the substrate. The mucus contents of the colon were determined by the Alcian blue dye binding method. Vascular permeability was estimated using 4% Evans blue passage after i.p. injection of 0.05 mol/L acetic acid. RESULTS: In the mice treated with OP, colonic macroscopic scores (1.1 ± 0.4 vs 2.7, P < 0.01) and the total square area of damage (10 ± 2 vs 21 ± 7, P < 0.01) were significantly reduced when compared with the vehicle-treated colitis group. OP was shown to decrease the tissue myeloperoxidase activity in colons (42 ± 11 vs 112 ± 40, P < 0.01) and enhance the amount of mucus of colitis mice (0.9 ± 0.1 vs 0.4 ± 0.1, P < 0.01). The level of colonic malondialdehyde was noted to decrease in OP-pretreated mice (3.6 ± 0.7 vs 5.1 ± 0.8, P < 0.01). OP was found to decrease the inflammatory status of mice as was determined by reduction of vascular permeability (161 ± 34 vs 241 ± 21, P < 0.01). Adhesionof peritoneal neutrophils and macrophages was also shown to decrease after administration of OP (141 ± 50 vs 235 ± 37, P < 0.05).CONCLUSION: Thus, a preventive effect of pectin from the common cranberry, namely oxycoccusan OP, on acetic acid-induced colitis in mice was detected. A reduction of neutrophil infiltration and antioxidant action may be implicated in the protective effect of oxycoccusan.
基金Supported by the Three New Project of Forestry in Jiangsu Province(LYSX[2016]46)the"Three-updating"Project of Agricultural in Changzhou City
文摘The swinging type temporary immersion bioreactors system( S-TTBs) is a kind of new and advanced method of tissue culture. The efficient multiplication technology of blueberry( Vaccinium corymbosum) plantlets in S-TTBs was systematically studied through L_9( 3~4) orthogonal experiment with three factors( culture medium volume,swing angle,and inoculation density) and completely randomized experiment with two factors( swing frequency and immersion time).The results showed that the optimal culture parameters were set as follows: the culture medium volume of 250 ml/bottle,the swing angle at 45°,and the inoculation density of 60 plantlets/bottle. The optimal swing frequency was 1 time/6 h,and the immersion time was set as 60 s.
基金supported by the National Natural Science Foundation of China(31170469,31170495 and 31470565)Technology division of Shaoxing(2013B70040).
文摘Aims Ericaceous plant species can host diverse fungi in their roots,including ericoid mycorrhizal fungi(ERMF),endophytes,pathogens and some species with unknown functions.However,how this diversity of fungi responds to different human disturbances is not well understood.Methods In this study,we examined the effects of different human disturbance on fungal diversity in hair roots of Vaccinium mandarinorum,an ericaceous plant.Fungal DNA was extracted from hair roots of V.mandarinorum and high-throughput sequencing was applied to detect the diversity of root-associated fungi along a human disturbance gradient in subtropical forests in Gutianshan National Nature Reserve(GNNR)in East China.The four forest types with different disturbance regime were:old growth forest(OGF),secondary forest with once cut(SEC I),secondary forest with twice cut(SEC II)and Cunninghamia lanceolata plantation(PLF).Important Findings The results showed that:(i)diverse fungal operational units(OTUs)were detected in hair roots of V.mandarinorum in the four types of forests,covering fungal phyla of Ascomycota,Basidiomycota,Chytridiomycota,Glomeromycota and Zygomycota;(ii)Community composition of root-associated fungi of V.mandarinorum in PLF was distinct from those in the other three forest types,and two types of secondary forests had similar fungal community composition;(iii)Different fungal families respond differently to human disturbances:fungal families with significant preference to OGF were ectomycorrhizal or saprophytic fungi while fungal families with higher relative abundance in PLF were plant pathogenic or saprophytic fungi;(iv)The first principal component(PC1)of plant community had a significant effect on composition of root-associated fungal community,while edaphic parameters showed no significant effect on fungal community composition in roots of V.mandarinorum.Our results help to better understand the responses of both ericaceous plants and their fungal partners to human disturbances and forest managements.
基金supported by the Natural Science Foundation of Zhejiang Province(grant LY22C160005)the National Natural Science Foundation of China(grant 31700224)+2 种基金Innovative Scientific and Technological Talents in Henan Province(no.20HASTIT041)the Outstanding Youth Foundation of Henan Province(no.202300410041)as well as a Nanyang Technological University startup grant and the Academy of Finland(decisions 318288 and 319947)to J.S.
文摘Vaccinium darrowii is a subtropical wild blueberry species that has been used to breed economically important southern highbush cultivars.The adaptive traits of V.darrowii to subtropical climates can provide valuable information for breeding blueberry and perhaps other plants,especially against the background of global warming.Here,we assembled the V.darrowii genome into 12 pseudochromosomes using Oxford Nanopore long reads complemented with Hi-C scaffolding technologies,and we predicted 41815 genes using RNA-sequencing evidence.Syntenic analysis across three Vaccinium species revealed a highly conserved genome structure,with the highest collinearity between V.darrowii and Vaccinium corymbosum.This conserved genome structure may explain the high fertility observed during crossbreeding of V.darrowii with other blueberry cultivars.Analysis of gene expansion and tandem duplication indicated possible roles for defense-and flowering-associated genes in the adaptation of V.darrowii to the subtropics.Putative SOC1 genes in V.darrowii were identified based on phylogeny and expression analysis.Blueberries are covered in a thick cuticle layer and contain anthocyanins,which confer their powdery blue color.Using RNA sequencing,we delineated the cuticle biosynthesis pathways of Vaccinium species in V.darrowii.This result can serve as a reference for breeding berries whose colors are appealing to customers.The V.darrowii reference genome,together with the unique traits of this species,including its diploid genome,short vegetative phase,and high compatibility in hybridization with other blueberries,make V.darrowii a potential research model for blueberry species.
文摘Objective: To study the protective effect of anthocyanins extracted from Vaccinium Uliginosum(VU)on retinal 661W cells against microwave radiation induced retinal injury. Methods: 661W cells were divided into 6 groups, including control, model [661W cells radiated by microwave(30 mW/cm2, 1 h)] and VU groups [661W cells pretreated with anthocyanins extracted from VU(25, 50, 100 and 200 μg/mL, respectively) for 48 h, and radiated by microwave 30 mW/cm2, 1 h]. After treatment with different interventions, the cell apoptosis index(AI)was determined using Heochst staining;contents of malonaldehyde(MDA), glutataione(GSH), and activity of superoxide dismutase(SOD) were measured. mRNA expressions of nuclear factor erythroid 2-related factor 2(Nrf2) and heme oxygenase 1(HO-1) were detected by real time quantitative polymerase chain reaction, and the expression of HO-1 protein was examined by Western blot analysis. Nucleus and cytoplasm were separated and Nrf2 protein expression was further verified by Western blot analysis. Results: There was significant difference in AI among the groups(F=322.83, P<0.05). Compared with the control group, AI was significantly higher in the model group and was lower in 4 VU-pretreated groups(P<0.05). Linear regression analysis showed the decline of AI was in a dose-dependent manner with VU treatment(r=0.8419, P<0.05). The MDA and GSH contents of 661W cells in VU-treated groups were significantly lower than the model group(P<0.05). Compared with the model group, the SOD activity in the VU-treated groups(50, 100 and 200 μg/mL) was significantly higher(all P<0.05). The Nrf2 and HO-1 mRNA expressions were slightly increased after irradiation, and obviously increased in 100 μg/mL VU-treated group. After irradiation, the relative expressions of HO-1 and Nrf2 proteins in nucleus were slightly increased(P<0.05), and the changes in cytoplasm were not obvious,whereas it was significantly increased in both nucleus and cytoplasm in the VU treatment groups. Conclusions:Anthocyanins extracted from VU could reduce apoptosis, stabilize cell membrane, and alleviate oxidant injury of mouse retinal photoreceptor 661W cells. The mechanism might be through activating Nrf2/HO-1 signal pathway and inducing HO-1 transcription and translation.