A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue...A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO2 enrichment, sucrose-reduced medium, and light intensity of 60 mM m-2·s-1. Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adaptability and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a photomixotrophic stage in the vitroplantlets cultured in TIBs.展开更多
The swinging type temporary immersion bioreactors system( S-TTBs) is a kind of new and advanced method of tissue culture. The efficient multiplication technology of blueberry( Vaccinium corymbosum) plantlets in S-TTBs...The swinging type temporary immersion bioreactors system( S-TTBs) is a kind of new and advanced method of tissue culture. The efficient multiplication technology of blueberry( Vaccinium corymbosum) plantlets in S-TTBs was systematically studied through L_9( 3~4) orthogonal experiment with three factors( culture medium volume,swing angle,and inoculation density) and completely randomized experiment with two factors( swing frequency and immersion time).The results showed that the optimal culture parameters were set as follows: the culture medium volume of 250 ml/bottle,the swing angle at 45°,and the inoculation density of 60 plantlets/bottle. The optimal swing frequency was 1 time/6 h,and the immersion time was set as 60 s.展开更多
The rapid propagation of plantlets of Vaccinium corymbosum in vitro was studied by using the stem-segment with buds as the explant.The optimal culture conditions were set as follows:1)for the initial culture,the expla...The rapid propagation of plantlets of Vaccinium corymbosum in vitro was studied by using the stem-segment with buds as the explant.The optimal culture conditions were set as follows:1)for the initial culture,the explants were sterilized with alcohol,sap nation of cresol and HgCl2,and then cultured on WPM medium containing ZT 3.0 mg·L-1 and 3% sucrose;2)the medium for clump bud induction was WPM + ZT 2.0 mg·L-1 + 3% sugar;3)the medium for robust shoot was WPM + ZT 0～1.0 mg·L-1 + 3% sugar;4)for adventitious root induction from shoots,the medium was 1/2 WPM liquid nutritions containing IBA 1.0～5.0 mg·L-1 and 2% sugar,in which either vermiculite,filter paper or sterilized cotton were added,and shoots were cultured under a photoperiod of 12 h illumination and 12 h darkness alternatively after 14 days in darkness;5)the medium for transplanting was turfy-soil and mosses（V∶V= 1∶1）.展开更多
文摘A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO2 enrichment, sucrose-reduced medium, and light intensity of 60 mM m-2·s-1. Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adaptability and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a photomixotrophic stage in the vitroplantlets cultured in TIBs.
基金Supported by the Three New Project of Forestry in Jiangsu Province(LYSX[2016]46)the"Three-updating"Project of Agricultural in Changzhou City
文摘The swinging type temporary immersion bioreactors system( S-TTBs) is a kind of new and advanced method of tissue culture. The efficient multiplication technology of blueberry( Vaccinium corymbosum) plantlets in S-TTBs was systematically studied through L_9( 3~4) orthogonal experiment with three factors( culture medium volume,swing angle,and inoculation density) and completely randomized experiment with two factors( swing frequency and immersion time).The results showed that the optimal culture parameters were set as follows: the culture medium volume of 250 ml/bottle,the swing angle at 45°,and the inoculation density of 60 plantlets/bottle. The optimal swing frequency was 1 time/6 h,and the immersion time was set as 60 s.
文摘The rapid propagation of plantlets of Vaccinium corymbosum in vitro was studied by using the stem-segment with buds as the explant.The optimal culture conditions were set as follows:1)for the initial culture,the explants were sterilized with alcohol,sap nation of cresol and HgCl2,and then cultured on WPM medium containing ZT 3.0 mg·L-1 and 3% sucrose;2)the medium for clump bud induction was WPM + ZT 2.0 mg·L-1 + 3% sugar;3)the medium for robust shoot was WPM + ZT 0～1.0 mg·L-1 + 3% sugar;4)for adventitious root induction from shoots,the medium was 1/2 WPM liquid nutritions containing IBA 1.0～5.0 mg·L-1 and 2% sugar,in which either vermiculite,filter paper or sterilized cotton were added,and shoots were cultured under a photoperiod of 12 h illumination and 12 h darkness alternatively after 14 days in darkness;5)the medium for transplanting was turfy-soil and mosses（V∶V= 1∶1）.
