The Asiatic hybrid lily(Lilium spp.)is a horticultural crop with high commercial value and diverse anthocyanin pigmentation patterns.However,the regulatory mechanism underlying lily flower color has been largely unexp...The Asiatic hybrid lily(Lilium spp.)is a horticultural crop with high commercial value and diverse anthocyanin pigmentation patterns.However,the regulatory mechanism underlying lily flower color has been largely unexplored.Here,we identified a WRKY transcription factor from lily tepals,LhWRKY44,whose expression was closely associated with anthocyanin accumulation.Functional verification indicated that LhWRKY44 positively regulated anthocyanin accumulation.LhWRKY44 physically interacted with LhMYBSPLATTER and directly bound to the LhMYBSPLATTER promoter,which enhanced the effect of the LhMYBSPLATTER-LhbHLH2 MBW complex activator on anthocyanin accumulation.Moreover,EMSA and dual-luciferase assays revealed that LhWRKY44 activated and bound to the promoters of gene LhF3H and the intracellular anthocyanin-related glutathione S-transferase gene LhGST.Interestingly,our further results showed that LhWRKY44 participated in light and drought-induced anthocyanin accumulation,and improved the drought tolerance in lily via activating stress-related genes.These results generated a multifaceted regulatory mechanism for the LhWRKY44-meditaed enhancement by the environmental signal pathway of anthocyanin accumulation and expanded our understanding of the WRKY-mediated transcriptional regulatory hierarchy modulating anthocyanin accumulation in Asiatic hybrid lilies.展开更多
By finger chromatogram of HPLC, 16 anthocyanins have been detected from the pigment of the fruit of Vaccinium ashei Reade which were introduced in Nanjing from the United States. Fourteen anthocyanins have been identi...By finger chromatogram of HPLC, 16 anthocyanins have been detected from the pigment of the fruit of Vaccinium ashei Reade which were introduced in Nanjing from the United States. Fourteen anthocyanins have been identified, among them 12 are -3-O-galactosides, glucosides, and arabinosides of delphinidin, cyanidin, petunidin, and malvidin, and 2 are -3-O-galactoside and glucoside of peonidin. Another 2 anthocyanins are under elucidation. It indicated that the components of the pigment extracted from V. ashei are similar to Myrtocyan extracted from V. myrtillus L., but the ratios are different and the contents are much lower.展开更多
基金supported by the National Natural Science Foundation of China(32172624,32172612,31672196)the Programs for National Key R&D Plan(2019YFD1001002).
文摘The Asiatic hybrid lily(Lilium spp.)is a horticultural crop with high commercial value and diverse anthocyanin pigmentation patterns.However,the regulatory mechanism underlying lily flower color has been largely unexplored.Here,we identified a WRKY transcription factor from lily tepals,LhWRKY44,whose expression was closely associated with anthocyanin accumulation.Functional verification indicated that LhWRKY44 positively regulated anthocyanin accumulation.LhWRKY44 physically interacted with LhMYBSPLATTER and directly bound to the LhMYBSPLATTER promoter,which enhanced the effect of the LhMYBSPLATTER-LhbHLH2 MBW complex activator on anthocyanin accumulation.Moreover,EMSA and dual-luciferase assays revealed that LhWRKY44 activated and bound to the promoters of gene LhF3H and the intracellular anthocyanin-related glutathione S-transferase gene LhGST.Interestingly,our further results showed that LhWRKY44 participated in light and drought-induced anthocyanin accumulation,and improved the drought tolerance in lily via activating stress-related genes.These results generated a multifaceted regulatory mechanism for the LhWRKY44-meditaed enhancement by the environmental signal pathway of anthocyanin accumulation and expanded our understanding of the WRKY-mediated transcriptional regulatory hierarchy modulating anthocyanin accumulation in Asiatic hybrid lilies.
文摘By finger chromatogram of HPLC, 16 anthocyanins have been detected from the pigment of the fruit of Vaccinium ashei Reade which were introduced in Nanjing from the United States. Fourteen anthocyanins have been identified, among them 12 are -3-O-galactosides, glucosides, and arabinosides of delphinidin, cyanidin, petunidin, and malvidin, and 2 are -3-O-galactoside and glucoside of peonidin. Another 2 anthocyanins are under elucidation. It indicated that the components of the pigment extracted from V. ashei are similar to Myrtocyan extracted from V. myrtillus L., but the ratios are different and the contents are much lower.
